Research Facilities

Imaging

The Imaging Facilities comprise a suite of confocal, 2-photon luminescence, wide field and conventional imaging systems. These systems will cover most imaging requirements for cells and tissues. There is a range of upright and inverted systems with various stage configurations. Environmental chambers are available on some systems making it possible to perform experiments over long time courses. More specialised imaging requirements including FRET, FLIM and FRAP can also be performed on some of the systems. A new FLIM system has no been installed on one of the 2-photon systems and a new TIRF-Confocal system is due to installed after the Easter break. The main Facilities are currently located on the 5th floor of the Rockefeller Building and a smaller unit is located on the 1st floor of the Medical Sciences Building that houses 3 confocal microscopes.

The Facility includes the Digital Imaging Unit, covering photography, illustration, page layout, scanning and the use of the Adobe Illustrator and Photoshop programmes. 

View the Imaging Facilities website here.


Contacts

Academic Leads:

Professor David Becker: d.becker@ucl.ac.uk

Professor Michael Duchen: m.duchen@ucl.ac.uk

Confocal Unit Support Staff:  
Imaging Facilites Manager: Dr Christopher Thrasivoulou: c.thrasivoulou@ucl.ac.uk
Daniel Ciantar:  d.ciantar@ucl.ac.uk
Tim Robson:  ucgabrd@ucl.ac.uk

Digital Imaging:

Jane Pendjiky:  a.pendjiky@ucl.ac.uk
External Phone: 0207 679 0904
Internal Phone: x40904

Confocal Support Staff Contact in Medical Sciences Building

Sam Ranasinghe sam.ranasinghe@ucl.ac.uk

phone: 020 7679 3206
internal-phone: 33206


Location

Centre for Cell & Molecular Dynamics
5th Floor
Rockefeller Building
21, University Street

and

1st Floor Medical Sciences Building


List of Equipment
Name
 Microscope Type
Excitation Multi-photon
Stage Incubator Software
Notes
MPuv
Upright; SP2  351; 364; 458; 476; 514; 543; 633  Y  Software-
controlled

 Y  LCS  
MP2 Upright; SP2 458; 476; 488; 514; 543; 633
Y
Fixed
Y
LCS
Water-immersion
SPuv Upright; SP1 476; 488; 568; 633
N
  N
LCS
 
SP2 Upright; SP2 458; 476; 488; 514; 543; 633
N
  N
LCS
 
Pspyglass
(SPf)


Upright; SP1 458; 488; 568; 633
N
Fixed
N
LCS
Water-immersion
 Pseemore
(SPinv)

Upright/
Inverted; SP1
458; 488; 568; 633
N
  N
LCS
 
 SPE1 Upright; SPE 405; 488; 532; 635
N
  N
  Single detection channel. Seq. Scan only for multiple fluorophores
 SPE2 Upright; SPE 405; 488; 532; 635
N
  N
  Single detection channel. Seq. Scan only for multiple fluorophores
 SPEinv Upright; SPE 405; 488; 532; 635
N
  N
  Single detection channel. Seq. Scan only for multiple fluorophores
 2P  Zeiss Upright
 488; 543; 633; 2-P Infra-red tunable
 Y    N  Zeiss
 Medical Sciences Building
 UV  Zeiss Inverted
 351; 364; 488; 543; 633
 N    Y   Zeiss  Medical Sciences Building
 BD Zeiss Inverted
405; 488; 543; 633
 N    N   Zeiss  Medical Sciences Building

Techniques and Capabilities

All the Confocal microscope systems are capable of:

Timelapse
3D Projections (Max; Av; Transparent)
Quantification (e.g. histogram, line profiles)
FRAP
FRET

Other Facilities:
FLIM
TIRF
Imaris
ImageJ plugins
Zeiss inverted

In addition we can offer other Imaging services: 
Image analysis service – ImageJ, Imaris (3D Rendering package) and KS400 image analysis software.

For information about any of these services please contact: Chris Thrasivoulou: c.thrasivoulou@ucl.ac.uk or Daniel Ciantar:  d.ciantar@ucl.ac.uk or extension 40904

Flatbed scanning of prints, negatives and transparencies; page layout; original artwork and design and photography. 

For information about any of these services please contact Jane Pendjiky on External Phone: 0207 679 0904, Internal Phone: x40904, E-mail:  a.pendjiky@ucl.ac.uk


Costs/Charges

Confocal Microscopes:

  • Biosciences ~ All confocal microscopes: £20.00 per hour.
  • Non-Biosciences ~ All confocal microscopes: £28.00 per hour.
  • Overnight: £10.00 per hour (must be a minimum of eight hours' usage).
  • ALL new users will be required to undergo a training session at a cost of £150.00.
  • Wide-field fluorescence/brightfield with environmental chamber time-lapse: £10.00 per half day.

    Please phone 020 7679 0904 (external) or x40904 (internal) to book this microscope.

Photographic/Illustration work:  £5.00 per hour.


Training
Training is given in use of the Confocal microscopes as and when required by individual users.  In addition the facility runs Post Graduate courses in basic and advanced Confocal microscopy.

Tuition and assistance can also be given in Adobe Illustrator and Adobe Photoshop.  We also run Post Graduate one-day workshops in Photoshop via the Robert’s Skills programme in conjunction with the Graduate School.

CCMD Confocal Imaging Web-Site


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Page last modified on 07 apr 11 16:19