Farfield dual polarisation interferometry Facility
Macromolecules can change shape when immobilised on a sensor surface and then subsequently treated with different buffers, small ligands or other macromolecules. These are measurable using DPI. This approach can often provide vital clues on bimolecular mechanisms, especially when considering interactions involving proteins immobilised on cell surfaces. Information on dissociation constants is also obtained. Apart from the need to immobilise the partner on a chip surface, the method is artefact-free and does not require the use of any labelling.

The Farfield dual polarisation interferometer instrument (purchased in April 2009) was funded by a CIF bid made jointly between Prof Steve Perkins and Prof John Ward in Structural and Molecular Biology.

It should be noted that we possess analytical ultracentrifuge equipment which makes complementary measurements in solution (see “AUC”) and surface Plasmon resonance which also makes measurements on a sensor chip (see “SPR”).

Contacts
Please email Jayesh Gor in the first instance:
Academic (who should often be the first point of call, to advise on feasibility and experimental plan) – Prof Steve Perkins (Structural and Molecular Biology):
020 7679 7048 (via Hearing Assistant) s.perkins@ucl.ac.uk
Technician (day-to-day and practical issues) – Jayesh Gor: 020 7679 2989
j.gor@ucl.ac.uk

Location
Room SB16 in the Darwin Subbasement.

List of Equipment
Farfield dual polarisation interferometer

Linux server with 4 Tb disk capacity for data archive.

Techniques and capabilities
 Real-time data following structural events during molecular function and interaction:

•    Mechanisms of disease action
•    Protein and biomolecule interactions
–    With other macromolecules (proteins, DNA, lipids, sugars)
–    With small molecules (inhibitors, metal ions)
•    Aggregation and inhibition
•    Protein folding and conformational changes
•    Polymer, surfactant, protein interactions
•    Lipids and membrane proteins

Costs/Charges
Negotiable. We usually charge £60 per day (+ VAT if required) for maintenance to those with grant support. Users should purchase their own sensor chips. A typical project carried out to completion may cost about £500-£1000. Please contact us if you need costs for a grant application.

Training
At present (March 2013), the instrument is in routine use by members of our group.  We anticipate that either we will run the samples for you, or users will have to arrange for proper training. Data processing is the users’ responsibility and we can usually provide users with either access multiuser PC’s or with their data and the necessary software. The manuals are available as PDF files.

Publications
At present, we would suggest a search for reviews using Google Scholar

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Page last modified on 05 jun 13 15:45