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UCL Department of Biochemical Engineering

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Dr Alexandros Kiparissides

Dr Alexandros Kiparissides

Lecturer

Dept of Biochemical Engineering

Faculty of Engineering Science

Joined UCL
11th Mar 2015

Research summary

Alexandros (Alex) Kiparissides is a Lecturer in Biochemical Engineering at University College London, having joined UCL in 2015. His current research focuses on the development of novel computational approaches able to integrate, organize and guide experimental (and modeling) information across multiple scales, i.e., all the way from metabolic regulation to bioprocess monitoring and control. Thus, his research explores the boundaries between traditional Process Systems Engineering, Systems Biology and Applied Biotechnology

Alex obtained a Diploma in Chemical Engineering from the Aristotle University of Thessaloniki and got his PhD in Bioprocess Systems Engineering from Imperial College London. In summary, the primary contribution of his thesis was the integration of wet-lab experiments, mathematical modeling, model analysis techniques, design of experiments and model based optimization methods into a formalized approach to bio-systems research and development (Kiparissides et al, 2011b). The versatility and applicability of the proposed methodology have been demonstrated in a number of publications, spanning a relatively broad range of applications, including: (a) the production of recombinant proteins in hybridoma cell lines (Ho et al., 2012, Kiparissides et al., 2015) (b) the bioremediation of aromatic pollutants by P.Putida (Koutinas et al, 2010, 2011a&b), (c) the regulation of stem cell differentiation (Kiparissides et al, 2011a; Phan et al., 2013) and (d) the effect of process conditions on the expansion of murine embryonic stem cells (Yeo et al. 2013).

Subsequently Alex was awarded with a post-doctoral fellowship at the École Polytechnique Fédérale de Lausanne (EPFL) in the lab of Vassily Hatzimanikatis. There he investigated the allocation of uncertainty within large scale metabolic networks and how it can affect the identification and evaluation of potential targets for Metabolic Engineering. His work combined Markov Chain based sampling algorithms, Global Sensitivity Analysis, Design of Experiments and thermodynamics-based Metabolic Flux Analysis in order to enumerate and evaluate intracellular flux states consistent with the studied physiology. He worked in collaboration with BP on the development of Metabolic Engineering strategies towards the increase of ethanol production by the “in-house” industrial yeast strain.

He is currently leading research projects on recombinant protein production in mammalian cell cultures and on the effects of the bioprocessing environment on algal cell culture efficiency. The general theme of his research is the development of an integrated methodology combining Multi-scale Modelling, Metabolic Engineering and wet-lab experiments in order to:

(i)             Better understand cell physiology and metabolism under industrial bioprocessing conditions

(ii)            Identify key parameters affecting culture efficiency at the metabolic, bioprocess and reactor design level

(iii)           Enable the model based design and optimisation of industrial scale bioreactors

Biography

Award Year 

Qualification

Institution

2012

Doctor of Philosophy (PhD)

Imperial College London

2006

Diploma of Chemical Engineering

Aristotle University of Thessaloniki

Publications