back to   Richardson home page       Kessaris home page                last updated September 2010

 

Request transgenic mice from the Bill Richardson/ Nicoletta Kessaris labs

 

We thank the UK Medical Research Council, The Wellcome Trust, the European Research Council, the Biotechnology and Biological Sciences Research Council, the US National Institutes of Health and The Royal Society for past and present support.                 

 

We have generated a number of transgenic strains, mainly BAC or PAC transgenics by pronuclear injection.   If you wish to obtain these please follow the steps below.

 

  1. email Bill Richardson  and/or Nicoletta Kessaris (see table below) outlining your proposed project in a few sentences.

 

  1. Please note that our animal facility is currently testing positive for Mouse Hepatitis Virus (MHV) which could create a problem for your animal facility.  Please first check with your animal facility that they can receive the mice before we initiate the export process. The latest Health Reports for our animals can be found here: Health report Mar 08, Health report Oct 08, MHV screen Jan 09, Health report May 09 room 1, Health report May 09 room 2, Health report June 10 room 1.  Further queries about mouse health status or husbandry should be directed to Nick Davies.   Here are answers to some Frequently Asked Questions.

 

  1. Complete the mouse request form and email it to our transgenic facility manager Ulla Dennehy

 

  1. Download the relevant Material Transfer Agreement (MTA) from the table below, sign and return direct to UCL Business (address is on the MTA).

 

  1. For shipments outside the UK please download the Home Office Transfer Form and complete/amend the highlighted sections.  Then email it to Ulla Dennehy for signature (you do not need to sign). We try to ship mice as soon as they are ready (usually within 3-4 months) but please allow a time window of 1 year for the shipment on the HO transfer form.

 

  1. For shipments within the UK, please download the UK Home Office Transfer Form, complete/amend the highlighted sections and return by post to Ulla Dennehy, Wolfson Institute for Biomedical Research, UCL, Gower Street, London WC1E 6BT.   Alternatively, you might already have permission written into your Project Licence to import/export animals within the UK, in which case you do not need to complete the transfer form – but please let Ulla Dennehy know.

 

We normally ship three (3) males of breeding age. We regret that we cannot routinely ship embryos.

 

Please note that we depend on you to pay costs.  Our facility routinely uses Harlan UK for the shipment– unfortunately, we cannot use other couriers.  Shipment is organized by Nick Davies

 

The following mice are available: 

 

Strain

Animal code

Contact

Vector

Made by

References

MTA

Genotyping protocol

Comments/

expression pattern

Dbx1-iCre

 H142

Kessaris cc Richardson

 PAC

Fogarty/Kessaris

1, 4, 5

Dbx1-iCre

Dbx1-iCre or iCre

Cortico-striatal boundary, spinal cord p1, p2, dP6, dP5

Dlx1-lox-Venus-lox

A666

Kessaris

BAC

Rubin/Alfonsi/

Kessaris

16 (suppl info)

Dlx1-Venus

Dlx-Venus

Forebrain

Emx1-iCre

 H151

Kessaris cc Richardson

PAC

Fogarty/Kessaris

3, (suppl info),  4, 5

Emx1-iCre

Emx1-iCre or iCre or use UV lamp at P0-P1*

Cortical neuroepithelium

Emx1-CreERT2

 G199

Kessaris cc Richardson

PAC

Fogarty/Kessaris

3, 5

Emx1-CreERT2

Emx1-CreERT2

Cortical neuroepithelium

Fgfr3-iCreERT2

 K121

Kessaris cc Richardson

PAC

Mitsumori/Kessaris

7 (suppl info),

13 (suppl info 1, 2)

Fgfr3-iCreERT2

iCre

Neuroepithelial cells, SVZ cells, protoplasmic and fibrous astrocytes

Gsh2-iCre

 H153

Kessaris cc Richardson

PAC

Kessaris

3, (suppl info),  4, 5, 10, 12

Gsh2-iCre

Gsh2-iCre or iCre

LGE/MGE/CGE

Lhx6-iCre

 N104

Kessaris

BAC

Kessaris

4, 6, 16 (suppl info), 17

Lhx6-iCre

iCre or use UV lamp at early stages*

MGE

Msx3-iCre

 K133

Kessaris cc Richardson

PAC

Fogarty/Kessaris

Unpublished

Msx3-iCre

Msx3-iCre or iCre or use UV lamp at early stages*

Dorsal spinal cord dP1, dP2, dP3, dP4 and neural crest

Nkx2.2-CreERT2

 H101

Kessaris cc Richardson

PAC

Taveira-Marques/Kessaris

Unpublished

Nkx2.2-CreERT2

Nkx2.2-CreERT2

Ventral spinal cord p3

Nkx2.1-iCre

 G178

Kessaris cc Richardson

PAC

Kessaris

3, (suppl. info),  4, 10, 12, 16 (suppl info), 17

Nkx2.1-iCre

Nkx2.1-iCre or iCre

MGE

Nkx2.1-iCre-ERT2

 K185

Kessaris cc Richardson

PAC

Kessaris

Unpublished

Nkx2.1-iCre-ERT2

Nkx2.1-iCre or iCre

MGE

Nkx6.2-iCre

 L129

Kessaris

BAC

Kessaris

4

Nkx6.2-iCre

iCre

LGE/MGE boundary, spinal cord

Olig2-Cre

 E193

Richardson

Knock-in

Rowitch/Kessaris

3, (suppl. info), 

14

Olig2-Cre

Olig2-Cre

Spinal cord pMN, oligodendrocyte lineage

Olig2-NLS-eGFP

 H152

Richardson

PAC

Callard/Kessaris

Unpublished

Olig2-NLS-eGFP

Olig2-GFP

Spinal cord pMN, oligodendrocyte lineage

Pdgfra-CreERT2 

 H126

Richardson

PAC

Jamen/Kessaris

7 (suppl info),

15

Pdgfra-CreERT2

 

Pdgfra-CreERT2

Oligodendrocyte progenitors postnatally (ectopic dorsal expression in embryo)

Sox1-iCreERT2

 

 K184

Kessaris cc Richardson

BAC

Kessaris

Unpublished

Sox1-iCreERT2

 

iCre

Embryonic neuroepithelium and adult neural stem cells

Sox10-Cre

 F155

Kessaris cc Richardson

PAC

Iannarelli/Kessaris

2, 8, 9, 11

Sox10-Cre

Sox10-Cre or use UV lamp at early stages*

Oligodendrocyte lineage, neural crest, Schwann cells

Sox10-lox-GFP-STOP-lox-DTA

 K152

Kessaris cc Richardson

PAC

Iannarelli/Kessaris

3, (suppl. info

Sox10- GFP-DTA

Sox10-DTA or use UV lamp at early stages

Oligodendrocyte lineage, neural crest, Schwann cells

Zic4-iCre

A611

Kessaris

BAC

Kessaris/Rubin

16 (suppl info)

Zic4-iCre

iCre

Septal telencephalic and diencephalic regions

 

* A UV lamp can be used to genotype these mice when a Cre reporter allele expressing a fluorescent protein is also present