This tutorial is intended to strengthen your understanding of gene cloning, PCR and Southern and northern (and even western) blotting. Before the tutorial put some thought into and attempt to answer the questions below. You will need to do some reading either from the web or possibly even from a book! Print out this page or use the sheet handed out in the lectures and bring your answers to the tutorial. Be prepared to discuss them.
(Not sure what any of these terms are? For a first clue look them up in the b241 glossary)
In days gone by, you might have made your reputation as a hot human geneticist by identifying the gene in which mutations cause a particular genetic disease 'a disease gene' e.g. the gene "for" cystic fibrosis.
Now you are more likely to investigate such questions as "What gene products interact with the gene product of the disease gene?".
A very clear description of the principles of Southern, northern and western blotting and gene cloning is to be found in the MIT Biology Hypertextbook at this page which may tell you all you need to know to answer the first questions.