Flow Cytometry - MoFlo
The MoFlo is an air-in-jet high-speed cell
sorter, equipped with three lasers that can measure up to 14 parameters
and sort 4 populations simultaneously. It is fitted with a CyClone,
the automated deposition unit, allowing single cell deposition for
cloning purposes. Sorting under sterile conditions can be done if
requested and sorted cells can be collected in several various vessels
(e.g. 1.5-50ml tubes, as well as 6-96-well and 10cm well plates).The
MoFlo temperature can be controlled over the sorting time from 4-37°C.
It can be set up with different nozzle sizes - from 50-150um - which
allows a nice range of cell sizes to be analyzed. The MoFlo typically
sorts at rates of 10,000 - 30,000 cells/second, with over 95% purity
and good recovery rates. The sorting mode can be altered to give
maximum purity, maximum recovery or maximum count accuracy - depending
on the request. Due to the generation of aerosols, a risk assessment
will be performed before accepting any samples.
The data can be analysed by the operator and given to you as a "ppt"
file, or you may request a FCS file so you can do further analysis
yourself, with the appropriate software (e.g. WinList, FlowJo, WinMdi).
If you don't have any of these: please ask.
Cell Cycle analysis
Access is at present allocated with equal
priority to WIBR users (3 days a week) and the Windeyer Institute
(2 days a week). Other users will be allocated time around these
prioritised users, if available. Please contact Tomas for further
020 7679 6895
- per hour
/ CI and MRC Centre Users
UCL and External Users
WIBR and MRC Request
External Request Form