Our facility uses state of the art instrumentation
from Biotage, in the form of the Pyromark MD system, to provide
high quality, affordable SNP/mutation detection and quantitative
DNA methylation analysis.
a simple and robust technique that sequences by synthesis. Using
a short PCR product as a template, bases are added systematically
and incorporated into a replicating DNA strand. Pyrosequencing utilises
a series of enzymatic reactions to detect pyrophosphate that is
released during base incorporation and uses the detection data to
establish the sequence of the template strand.
There are two main application areas that
our Pyromark MD system can be utilised for, SNP/mutation analysis
and DNA methylation studies.
Using the Pyromark MD system it is possible
to analyse single and multiple SNPs and insertion/deletion mutations
within 200bp stretches of the genome.
DNA Methylation Analysis
It is possible to quantitatively analyse
PCR products amplified from bisulphite treated genomic DNA for levels
of methylation at single or multiple CpG sites across the genome.
Further information about Pyrosequencing
and the capabilities of the instrumentation can be found here:
Pyrosequencing technology overview
PyroMark™MD instrument overview
Our service can be tailored to individual
requirements and may include all or a combination of the following:
• Assay design
• Oligonucleotide synthesis
• Bisulphite treatment of genomic DNA (methylation study only)
• Assay optimisation
• Sample assay
• Interpretation and troubleshooting of results
Please contact us to discuss your individual
020 7679 6719
and Oligo Synthesis