The Leica functions as a 3-colour, UV and visible confocal microscope in one system,
utilizing fiber coupled
UV, VIS and IR lasers (allowing rapid switching between laser-firing).
There is even a "Design-a-filter" application for ultimate
flexibility, or new dyes and difficult dye combinations, and allows
easy wavelength selection from pre-programmed spectra of all popular
dyes. The excitation wavelengths commonly used are:
Wavelenght (nm) | Dye |
543 | TRITC |
488 | FITC |
366 | Cy5 |
458 | GFP |
190-370 | DAPI |