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Wolfson Institute for Biomedical Research

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The Leica functions as a 3-colour, UV and visible confocal microscope in one system,

utilizing fiber coupled UV, VIS and IR lasers (allowing rapid switching between laser-firing). There is even a "Design-a-filter" application for ultimate flexibility, or new dyes and difficult dye combinations, and allows easy wavelength selection from pre-programmed spectra of all popular dyes. The excitation wavelengths commonly used are:


Wavelenght (nm) Dye
543 TRITC
488 FITC
366 Cy5
458 GFP
190-370 DAPI
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What is confocal?