Wolfson Institute for Biomedical Research
The p53 tumour suppressor is a transcriptional activator that controls cell fate in response to various stresses. p53 can initiate cell cycle arrest, senescence and/or apoptosis via transactivation of p53 target genes, thus preventing cancer onset. Mutations that impair p53 usually occur in the core domain and negate the p53 sequence-specific DNA binding. Moreover, these mutations exhibit a dominant negative effect on the remaining wild-type p53. We have recently solved the first cryo electron microscopy structure of the full-length p53 tetramer bound to a DNA encoding p53 response element (RE) at a resolution of 21Å. While two core domains from both dimers of the p53 tetramer interact with DNA within the complex, the other two core domains remain available for binding another DNA site. This finding helps to explain the dominant negative effect of p53 mutants based on the fact that p53 dimers are formed co-translationally before the whole tetramer assembles; therefore, a single mutant dimer would prevent the p53 tetramer from binding DNA. The structure indicates that the Achilles’ heel of p53 is in its dimer-of-dimers organization, thus the tetramer activity can be negated by mutation in only one allele followed by tumourigenesis.
Figure 2. 3D structure of p53-DNA complex. Atomic structures of p53 core domains are docked into the 3D EM map. Two p53 dimers shown in blue and red. DNA is in orange.
The high EM densities are shown in light orange at 4σ.
Aramayo R, Sherman MB, Brownless K, Lurz R, Okorokov AL, Orlova EV. (2011) Quaternary structure of the specific p53-DNA complex reveals the mechanism of p53 mutant dominance. Nucleic Acids Res. Jul 14. [Epub ahead of print]; free access