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Flow
Cytometry - MoFlo
General
The MoFlo
is an air-in-jet high-speed cell sorter, equipped with three lasers
that can measure up to 14 parameters and sort 4 populations simultaneously.
It is fitted with a CyClone, the automated deposition unit, allowing
single cell deposition for cloning purposes. Sorting under sterile
conditions can be done if requested and sorted cells can be collected
in several various vessels (e.g. 1.5-50ml tubes, as well as 6-96-well
and 10cm well plates).
The MoFlo
temperature can be controlled over the sorting time from 4-37°C.
It can be set up with different nozzle sizes - from 50-150um -
which allows a nice range of cell sizes to be analyzed. The MoFlo
typically sorts at rates of 10,000 - 20,000 cells/second, with
over 95% purity and good recovery rates. The sorting mode can
be altered to give maximum purity, maximum recovery or maximum
count accuracy - depending on the request. Due to the generation
of aerosols, a risk assessment will be performed before accepting
any samples.
.
The data can be analysed by the operator and given to you as a
"ppt" file, or you may request a FCS file so you can
do further analysis yourself, with the appropriate software (e.g.
WinList, FlowJo, Cyclher or WinMdi). If you don't have any of
these: please ask.
Applications
Cell Cycle
analysis
Apoptosis
analysis
Multicolour
analysis
Functional
analysis
Cell Sorting
Booking
Access is
at present allocated with equal priority to WIBR users (3 days
a week) and the Windeyer Institute (2 days a week). Other users
will be allocated time around these prioritised users, if available.
Please contact Lola for further information.
Contact
Us
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Lola
Martinez
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020
7679 6827
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d.martinez@ucl.ac.uk
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Prices
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WIBR
and MRC Centre Users
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Other
UCL and External Users
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Use
of MoFlo
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40.83
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75.00
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per
Hour
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Downloads
Cell
Cycle Basics
Links
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