DNA Sequencing

General

We run a DNA sequencing service using two Beckman Coulter CEQ 8000 genetic analysis systems. Our service has been expanding year on year since it began in 2000, and continues to grow. We believe that this is due to our high quality results, fast turn around, comprehensive result analysis and feedback.

We offer:

• Turn around time of 2-3 working days
• Variety of protocols to successfully sequence 'difficult' templates
• Minimum of two control samples run with every plate
• Full explanation of results, with advice on how to improve results
• Free repeat if there is a possibility of error on our part (very rare!)
• Heterozygote detection for PCR products
• Sequence comparison
• Please follow the instructions and use the booking form below.

In addition to this we now offer:

Premium Service

Same day service! Samples submitted by 9.30am will be analysed and results sent by 5.30pm. For more details, click here.

Budget Service

We now run diluted Mastermix reactions for customers wishing to keep costs down, whilst maintaining good quality sequencing. For more details, click here.

Technical

Template

The quantity and quality of template DNA should be determined by agarose gel electrophoresis, or you may use the NanoDrop spectrophotometer which we have in our lab. This is very important to ensure good results. It is also very important that the template is re-suspended in dH2O.

We recommend -

QIAGEN Qiawell and QIAprep (Mini-Prep) DNA isolation protocols (dsDNA and ssDNA).

QIAGEN Qiaquick PCR purification protocol (PCR products).

We require 12µl of template per reaction provided in 1.5ml eppendorf tubes at a concentration of:

9-16fmoles/µl if cloned in vector

4-8fmoles/µl if purified PCR product

Please refer to DNA Concentration Table.

Primers

We have 7 standard primers in stock. Please check the sequences to be sure they are correct for your requirements. If using your own primers Please provide them at a concentration of 2-5pmoles/µl, allowing 6µl per reaction. Please follow these primer design rules to ensure successful reactions.

Request form

Results

The results are sent out as either .scf or text files, or both. These are emailed to users as soon they have been analysed, along with comprehensive feedback. For added confidence in our service, we will happily send users the results from the control reactions associated with their samples. Users wishing to discuss results can call us on one of the numbers below, or email us. We will respond on the same day. Please refer to our troubleshooting guide.

Contact Us

Prices

Downloads

WIBR Request form.

UCL Request form. Please send an IDT with the form.

External Request form. Please send a Purchase Order with the form.

Feedback form.

DNA concentration table.

Primer design rule.

How does sequencing work?

Links

Beckman homepage

Primer Calculator