Doing without antibiotics: the pMB1 origin of replication as a novel selectable marker in enteric bacteria
Plasmids are commonly used for the production of recombinant proteins or DNA for gene therapy and often an antibiotic-resistance gene is used as a selectable marker. There is now a move in the licensing bodies such as the FDA, to eliminate antibiotics from the growth media and resistance genes from plasmids in processes that make therapeutic products. It is important to try to develop novel, effective methods to select for plasmids without using antibiotics. The overall aim of the project is to construct an Escherichia coli host strain that will enable plasmid selection without the use of antibiotics and their resistance genes. This will be done using the antisense RNAs of the pMB1 origin of replication.