Prof Greg Towers
Our work aims to understand the molecular details of host virus interactions. We focus on human immunodeficiency virus type 1, the cause of AIDS in humans, but we also study other retroviruses as well as herpes viruses such as HSV-1, human cytomegalovirus and Kaposi’s sarcoma herpes virus and adenoviruses.We study host virus interactions because we believe that the new knowledge we find will be valuable in many ways. For example, we expect that a more detailed understanding of host virus interactions will help us to drug viral infection experimentally and therapeutically. We are developing novel inhibitors of viral infection that manipulate viruses’ ability to hide from innate immune pattern recognition receptors. We also aim to use our understanding of innate immune control of HIV-1 to develop novel gene therapy based approaches to treat HIV-1 infection.
We believe that viruses are very good cell biologists and by working out how they interact with their hosts we will discover new understanding of host cell processes. We also believe that one cannot truly appreciate the relationship between host and virus without a sound understanding of evolution. This is best illustrated by Lee Van Valen’s Red Queen hypothesis, which suggests that host and pathogen are locked in a genetic conflict in which both host and virus are obliged to continually evolve with each alternately gaining and losing the advantage.
We also study host virus interactions because it is a very competitive and well-funded area of research that is really good fun to work in.
+44 (0)203 108 2116
PostDocs and Senior Scientists
Dr Maria Teresa Rodriguez Plata
The innate immune system is the first line of defense against pathogens and HIV-1 has evolved strategies to evade it as a critical adaptation required for transmission and replication. I am interested in understanding the molecular details of the relationship between HIV-1 and the innate immune sensing in CD4+ T lymphocytes, the main target of HIV-1.
Dr Becky Sumner
I am interested in the interaction between viruses and the host innate immune system, and in particular how we can use these pathogens as tools to learn more about how innate immunity is triggered and how it controls virus spread. My previous work in the lab of Prof. Geoff Smith focused on innate immune evasion strategies of vaccinia virus, a member of the poxvirus family that was used to vaccinate against, and ultimately eradicate, smallpox. This large DNA virus dedicates a large proportion of its coding capacity to dampening host immunity and I was involved in characterising how a number of these proteins negatively regulate innate immunity and how this impacts virulence and the use of vaccinia virus as a vaccine vector. My current work in the Towers lab is focused on how HIV is detected by the innate immune system and the mechanisms and cofactors this virus employs to avoid recognition. Importantly, we hope that by understanding these viral evasion strategies in detail we may be able to design drugs that expose the virus to the host innate immune system, thus aiding viral clearance.
I am an industry trained chemist working in the lab of David Selwood and funded by an ERC Advanced Grant to Greg and David. I am designing and synthesising novel anti-viral inhibitors using a medical medicinal chemistry and structure based approach. We are aiming to develop novel small molecules and modified natural products which inhibit viral innate immune evasion strategies and reveal viruses to innate immune sensors. Importantly, many of our compounds target host, rather than viral proteins. This is a new paradigm for the treatment of viral infection, which we expect will be particularly effective for two reasons. Firstly, the virus will struggle to escape by mutation from a molecule that targets a host protein. Secondly, a large part of the antiviral effect comes from the activated innate immune system which is particularly effective at suppressing viral infection and again, difficult to avoid by mutation.
Dr Isobella Honeyborne
HIV-1 infected individuals can never completely clear their virus even when their immune system or antiviral drug therapies reduce the virus to undetectable levels. The nature of the "reservoir" of infected cells remains poorly understood although candidate cell populations such as memory CD4+ T cells have been suggested. I am interested in better understanding the nature of the antiviral reservoir and considering ways to target it for destruction. I am working with Jane Rasaiyaah to investigate the use of drugs that reveal HIV-1 to innate immune sensors and to consider what effect such drugs might have on viral reservoirs. We would like to know what would happen to the viral reservoir if we treat it with combinations of drugs that simultaneously cause cellular activation and reveal the virus to innate immune sensors. Such regimens may eventually have the capacity to eradicate the reservoir while preventing the virus from spreading to neighbouring cells.
Dr Katsiaryna Bichel
My interest is understanding the molecular details of the interactions between HIV-1 and its host and how these interactions control the processes of infection or lead to triggering of defensive innate immune responses. I take a biochemical approach to considering the details of these host and virus protein-protein interactions. I collaborate with the lab of Professor John Christodoulou at UCL and use NMR, as well as molecular biology, to consider my research questions.
Dr Carolina Ferreira
Despite significant progress in preventing mother-to-child transmission of HIV/AIDS, the number of newly infected children remains unacceptably high. I am working with Professor Waseem Qasim (UCL Institute of Child Health) and Professor Greg Towers on a gene therapy to treat HIV infection in children. We have developed gene therapy vectors that express a potent anti-HIV restriction factor based on TRIM-Cyclophilin proteins. These humanised proteins have been designed to mimic the TRIMCyp anti-HIV proteins that have evolved on at least two independent occasions in non-human primates. Selective pressure from pathogenic viruses has favoured the evolution of these fusion proteins and they act as powerful inhibitors of HIV-1 infection and potent activators of innate immune responses. Such human TRIM5CypA variants mediate robust inhibition of HIV-1 in vitro and in human-murine chimeric models of in vivo T cell engraftment, without evidence of mutagenic escape by the virus. Successful gene therapy could mean a single treatment cure for HIV infection in children.
Dr Christoffer Van Tulleken
The non-homologous end joining pathway (NHEJ) is known to circularize linear HIV DNA after reverse transcription. Prevention of this process by reduction of any of the components leads to apoptosis presumably due to a pro apoptotic signal activated by the DNA free ends. Because the circles formed are not productive, circularization may be a defensive act for the host but may also help the virus evade pattern recognition.
Previous experiments considering a role for the NHEJ pathway in HIV infection have been performed in cells which are unlikely to be competent for effective innate pattern recognition and so I am seeking to investigate the effect on HIV replication and innate immune stimulation of the NHEJ in T-Cell lines and human primary cells using siRNA knock downs and microarrays.
am studying the ability of Interferon to inhibit HIV-1 replication. I
am considering which restriction factors may play a role in the ability
of interferon treatment to suppress HIV-1 replication in primary human
monocyte derived macrophages.
mammalian genome is generally divided into coding (2%) and noncoding
DNA sequences. Around 40% of the noncoding DNA is derived from
retrotransposons, which are genetic elements that can amplify
themselves and are capable of integrating into new locations in the
genome. These elements have coevolved with their hosts and have been
linked to cell development and gene regulation, as well to congenital
defects or diseases such as cancer. I am interested
in exploring the regulation of endogenous retroelements in humans and
the pathogenesis of diseases linked to the their misregulation.
I am studying for a PhD between Public Health England and UCL with Tamyo Mbisa at PHE and Greg Towers at UCL. I am interested characterizing the biology of the earliest HIV-1 sequences that can be detected after infection. These viruses are called founder viruses and it has been shown that as few as a single founder clone can be responsible for HIV-1 transmission. Founder viruses appear to have different properties from our favorite HIV-1 clones such as NL4.3. We would like to better understand the unique features of founder viruses and to understand how these features contribute to transmission. With a better view of determinants of transmission we hope to better understand how to prevent transmission either through prophylaxis or vaccination.
Because HIV can transmit with a single founder sequence, I hypothesise that I will be able to determine when an individual became infected by measuring the genetic diversity of circulating virus. I propose to test this hypothesis by phylogenetic analysis of virus sequences derived from samples with known transmission times.
All viruses are obliged to evade or antagonise the intracellular innate immune system in order to replicate. Indeed, HIV-1 encodes accessory genes that antagonise the innate restriction factors that otherwise suppress infection. Vif antagonise APOBECs, Vpu antagonise tetherin and recently Nef has been shown to antagonise SERINC3/5. However, the role of the accessory protein Vpr has been obscure. By taking a multidisciplinary approach that combines molecular virology with structural biology, I am to elucidate the function of Vpr during HIV-1 infection and to understand how Vpr makes cells more permissive to HIV-1 replication.
Dr Doug Fink
I am an academic clinical fellow in the Towers lab. This means that I am a medical doctor gaining research experience with a view to undertaking a PhD. In particular I am interested in how the human body defends itself against viral and other infections. I am currently investigating these complex processes using retroviral accessory proteins as a tool for interrogating the innate immune system.
Page last modified on 20 may 16 14:14 by Jane Lorna Elizabeth Turner