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Publications

Weighted references (according to importance *, **, ***)

**This review reports the ‘Elongation first hypothesis’ of transcription regulation

Evolution of the RNA polymerases in the three domains of life

Werner F. and Grohmann D

Nature Reviews Microbiology. 2011 Feb

This is a review focussing on the role of the Rpo4/7 complex for RNAP function

Cycling through transcription with the RNA polymerase F/E (RPB4/7) complex: structure, function and evolution of archaeal RNA polymerase.

Grohmann D, Werner F.

Res Microbiol. 2010 Sep 21. [Epub ahead of print]PMID: 20863887 [PubMed - as supplied by publisher] Related citations


*This point of view paper describes how both RNAP subunits and transcription elongation factors target the RNAP clamp motif to regulate transcription

Hold on!: RNA polymerase interactions with the nascent RNA modulate transcription elongation and termination.

Grohmann D, Werner F.

RNA Biol. 2010 May-Jun;7(3):310-5. Epub 2010 May 26.PMID: 20473037 [PubMed - in process] Free PMC Article Free text Related citations


**This paper describes the use of two biophysical methods, fluorescence and electron paramagnetic resonance to characterise intermolecular proximity and dynamic conformational changes

RNA-binding to archaeal RNA polymerase subunits F/E: a DEER and FRET study.

Grohmann D, Klose D, Klare JP, Kay CW, Steinhoff HJ, Werner F.

J Am Chem Soc. 2010 May 5;132(17):5954-5.PMID: 20384325 [PubMed - indexed for MEDLINE] Related citations


***This article reports the first functional and structural characterisation of the universally conserved elongation factor Spt4/5 (called NusG in Bacteria)

Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif.

Hirtreiter A, Damsma GE, Cheung AC, Klose D, Grohmann D, Vojnic E, Martin AC, Cramer P, Werner F.

Nucleic Acids Res. 2010 Jul 1;38(12):4040-51. Epub 2010 Mar 2.PMID: 20197319 [PubMed - indexed for MEDLINE] Free PMC Article Free text Related citations


***This article describes the mechanism of the Rpo4/7 RNAP subcomplex during the elongation and termination phase of transcription

RNAP subunits F/E (RPB4/7) are stably associated with archaeal RNA polymerase: using fluorescence anisotropy to monitor RNAP assembly in vitro.

Grohmann D, Hirtreiter A, Werner F.

Biochem J. 2009 Jul 15;421(3):339-43.PMID: 19492989 [PubMed - indexed for MEDLINE]Free Article Related citations


This review describes the molecular mechanisms of archaeal RNA polymerase

Molecular mechanisms of archaeal RNA polymerase.

Grohmann D, Hirtreiter A, Werner F.

Biochem Soc Trans. 2009 Feb;37(Pt 1):12-7.PMID: 19143594 [PubMed - indexed for MEDLINE] Related citations


This news and views review describes the recently solved X-ray and cryoEM structure of archaeal RNA polymerase

Structural evolution of multisubunit RNA polymerases.

Werner F.

Trends Microbiol. 2008 Jun;16(6):247-50. Epub 2008 May 28.PMID: 18468900 [PubMed - indexed for MEDLINE] Related citations


This review describes the molecular anatomy of archaeal RNA polymerase

Structure and function of archaeal RNA polymerases.

Werner F.

Mol Microbiol. 2007 Sep;65(6):1395-404. Epub 2007 Aug 14. Review.PMID: 17697097 [PubMed - indexed for MEDLINE] Related citations


This article describes the function of the basal factor TF(II)B

Modulation of RNA polymerase core functions by basal transcription factor TFB/TFIIB.

Werner F, Wiesler S, Nottebaum S, Weinzierl RO.

Biochem Soc Symp. 2006;(73):49-58.PMID: 16626286 [PubMed - indexed for MEDLINE] Related citations


This article describes how basal transcription factors TF(II)B and TF(II)E directly modulate RNAP function during transcription initiation

Direct modulation of RNA polymerase core functions by basal transcription factors.

Werner F, Weinzierl RO.

Mol Cell Biol. 2005 Sep;25(18):8344-55.PMID: 16135821 [PubMed - indexed for MEDLINE]Free PMC Article Free text Related citations


**This article describes the application of a recombinant RNAP to dissect Ptr2-activated transcription

A fully recombinant system for activator-dependent archaeal transcription.

Ouhammouch M, Werner F, Weinzierl RO, Geiduschek EP.

J Biol Chem. 2004 Dec 10;279(50):51719-21. Epub 2004 Oct 14.PMID: 15485836 [PubMed - indexed for MEDLINE] Free Article Related citations


This article sheds light on the structural and functional conservation of RNAP stalk complexes across the classes of eukaryotic RNAPs

Structural and functional homology between the RNAP(I) subunits A14/A43 and the archaeal RNAP subunits E/F.

Meka H, Daoust G, Arnvig KB, Werner F, Brick P, Onesti S.

Nucleic Acids Res. 2003 Aug 1;31(15):4391-400.PMID: 12888498 [PubMed - indexed for MEDLINE] Free PMC Article Free textRelated citations


***This article describes the first successful in vitro reconstitution of a complex RNAP from recombinant subunits

A recombinant RNA polymerase II-like enzyme capable of promoter-specific transcription.

Werner F, Weinzierl RO.

Mol Cell. 2002 Sep;10(3):635-46.PMID: 12408830 [PubMed - indexed for MEDLINE] Related citations


***This article describes the first X-ray structure of the RNAP stalk

Structure of an archaeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex.

Todone F, Brick P, Werner F, Weinzierl RO, Onesti S.

Mol Cell. 2001 Nov;8(5):1137-43.PMID: 11741548 [PubMed - indexed for MEDLINE] Related citations


This article reports the identification and characterisation of two RNAP subunits in the archaeal domain

Archaeal RNA polymerase subunits F and P are bona fide homologs of eukaryotic RPB4 and RPB12.

Werner F, Eloranta JJ, Weinzierl RO.

Nucleic Acids Res. 2000 Nov 1;28(21):4299-305.PMID: 11058130 [PubMed - indexed for MEDLINE]Free PMC Article Free text Related citations