Prof Jonathan Ashmore

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Personal Profile

Name: Jonathan Ashmore Email: j.ashmore@ucl.ac.uk
Title: Prof Tel: 020 7679 2141
Department: Neuroscience, Physiology & Pharmacology Fax: 020 7813 0530
Position: Bernard Katz Professor of Biophysics Address: Department of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT
Research Domain: Basic Life Sciences, Experimental Medicine, Neuroscience, Personalised Medicine Web Page: Personal Web Page

Profile

Research Description

My work addresses a variety of cellular mechanisms of hearing. Using a variety of biophysical techniques I work on the mechanisms of electromotility in cochlear outer hair cells. Using confocal imaging I have been working on the mechanisms of synaptic release at the ribbon synapse of the inner hair cells, combining electrophysiology and multiphoton imaging. Using large scale computational models I work on the patterning of electrical current flow in the cochlea during stimulation by sound. More recently I have become interested in potassium channel function in early auditory processing and the nature and origin of tinnitus.

Research Activities

Cellular Mechanisms of Hearing

Potassium homeostasis in the cochlea and deafness

The active role of outer hair cells

Education Description

UCL Collaborators

Prof Tony Gardner-Medwin; Dr Sally Dawson

External Collaborators

Publications

    2012

    • Quick K, Zhao J, Eijkelkamp N, Linley JE, Rugiero F, Cox JJ, Raouf R, Gringhuis M, Sexton J, Abramowitz J, Taylor R, Forge A, Ashmore J, Kirkwood N, Kros CJ, Richardson G, Freichel M, Flockrzi V, Birnbaumer L, Wood JN (2012). TRPC3 and TRPC6 are essential for normal mechanotransduction in subsets of sensory neurons and cochlear hair cells. Open Biology, 2, - .
    • Mistrík P, Daudet N, Morandell K, Ashmore JF (2012). Mammalian prestin is a weak Cl⁻/HCO₃⁻ electrogenic antiporter.. J Physiol, 590(Pt 22), 5597 - 5610. doi:10.1113/jphysiol.2012.241448

    2011

    • Ashmore JF (2011). Pushing the envelope of sound.. Neuron, 70 Neuron.(6), 1021 - 1022.

    2010

    • Ashmore JF , Boutet de Monvel J , Petit C , Saffieddine S (2010). Synaptic release evoked by depolarization in mouse cochlear inner hair cells imaged in situ in the isolated temporal bone.
    • Mistrík P, Ashmore JF (2010). Reduced electromotility of outer hair cells associated with connexin-related forms of deafness: an in silico study of a cochlear network mechanism.. J Assoc Res Otolaryngol, 11(4), 559 - 571. doi:10.1007/s10162-010-0226-3
    • Ashmore JF (2010). The afferent synapse. In Fuchs PF (Ed.), The Ear (pp. 259 - 282). : Oxford University Press.
    • Culley S , Ashmore JF (2010). Identification of simultaneous calcium entry sites in cochlear inner hair cells of the adult mouse.
    • Ashmore J (2010). Cochlear amplification - Somatic or stereocilial forces? A first-person response. Hear.Res., , - .

    2009

    • Mistrik P, Ashmore J (2009). A Prediction for the Role of Gap Junction Mutations from a 3D Model of Ionic Circulation in the Cochlea.
    • Mistrik P, Ashmore J (2009). The role of potassium recirculation in cochlear amplification.. Current Opinion in Otolaryngology and Head and Neck Surgery, 17(5), 394 - 399. doi:10.1097/MOO.0b013e328330366f
    • Mistrik P, Ashmore J (2009). Using A Large Scale Computational Model To Study The Effect Of Longitudinal And Radial Electrical Coupling In The Cochlea.
    • Navarro-López J, Jiménez-Díaz L, Géranton SM, Ashmore JF (2009). Electrophysiological and molecular analysis of Kv7/KCNQ potassium channels in the inferior colliculus of adult guinea pig. Journal of Molecular Neuroscience, 37(3), 263 - 268. doi:10.1007/s12031-008-9130-2

    2008

    • Ashmore J (2008). Cochlear outer hair cell motility. Physiological Reviews, 88(1), 173 - 210. doi:10.1152/physrev.00044.2006
    • Mistrik P, Mullaley C, Mammano F, Ashmore J (2008). Three-dimensional current flow in a large-scale model of the cochlea and the mechanism of amplification of sound.. Journal of the Royal Society Interface, , - .

    2007

    • Mistrik P, Saffrey P, Mullaley P, Nobili R, Mammano F, Ashmore J (2007). A computationally based large scale model of potassium flow in the cochlea..
    • Hetherington J, Bogle IDL, Saffrey P, Margoninski O, Li L, Varela Rey M, Yamaji S, Baigent S, Ashmore J, Page K, Seymour RM, Finkelstein A, Warner A (2007). Addressing the challenges of multiscale model management in systems biology. doi:10.1016/j.compchemeng.2006.10.004
    • Sun Z, Finkelstein ACF, Ashmore J (2007). Using Ontology with Semantic Web Services to Support Modeling in Systems Biology. doi:10.1007/978-3-540-77010-7_5

    2006

    • Muallem DR, Ashmore JF (2006). An anion antiporter model of prestin, the outer hair cell motor protein. Biophysical Journal, 90(11), 4035 - 4045.
    • Navarro-Lopez J, Ashmore JF (2006). Whole-cell recording of neuronal populations in the inferior colliculus of the adult guinea pig.

    2005

    • Chambard JM, Ashmore JF (2005). Regulation of the voltage-gated potassium channel KCNQ4 in the auditory pathway.. Pflügers Archiv European Journal of Physiology, 450(1), 34 - 44. doi:10.1007/s00424-004-1366-2
    • Griesinger CB, Richards CD, Ashmore JF (2005). Fast vesicle replenishment allows indefatigable signalling at the first auditory synapse.. Nature, 435(7039), 212 - 215. doi:10.1038/nature03567

    2004

    • Griesinger CB, Richards CD, Ashmore JF (2004). Apical endocytosis in outer hair cells of the mammalian cochlea.. European Journal of Neuroscience, 20(1), 41 - 50.
    • Ashmore J, Gale J (2004). The cochlear amplifier.. Current Biology, 14(11), R403 - R404.
    • Griesinger CB, Richards CD, Ashmore JF (2004). Apical endocytosis in outer hair cells of the mammalian cochlea. EUR J NEUROSCI, 20(1), 41 - 50. doi:10.1111/j.1460-9568.2004.03452.x
    • Ashmore J (2004). Hearing: channel at the hair's end.. Nature, 432(7018), 685 - 686.

    2003

    • Chambard JM, Ashmore JF (2003). Sugar transport by mammalian members of the SLC26 superfamily of anion-bicarbonate exchangers.. The Journal of Physiology, 550(3), 667 - 677. doi:10.1113/jphysiol.2003.039321
    • Griesinger CB, Richards CD, Ashmore JF (2003). Two photon imaging of synaptic release in inner hair cells.

    2002

    • Griesinger CB, Richards CD, Ashmore JF (2002). FM1-43 reveals membrane recycling in adult inner hair cells of the mammalian cochlea. Journal of Neuroscience, 22(10), 3939 - 3952.
    • Ashmore JF, Chambard JM, Richmond S (2002). Cochlear transduction: from models to molecules and back again. Audiology and Neuro-Otology, 7(1), 6 - 8.
    • Ashmore J (2002). Biophysics of the cochlea - biomechanics and ion channelopathies. British Medical Bulletin, 63, 59 - 72.

    2001

    • Griesinger CB, Richards CD, Ashmore JF (2001). Two photon laser scanning confocal microscopy of FM1-43 uptake in the intact cochlea. BIOPHYS J, 80(1), 159A - 159A.
    • Ashmore JF, Mammano F (2001). Can you still see the cochlea for the molecules?. Current Opinion in Neurobiology, 11(4), 449 - 454.
    • Griesinger CB, Richards CD, Ashmore JF (2001). Two photon laser scanning confocal microscopy of FM1-43 uptake in the intact cochlea.
    • Ashmore JF, Charras G, Horton MA (2001). Combining atomic force microscopy and patch clamp in cochlear outer hair cells. BIOPHYS J, 80(1), 306A - 306A.
    • Lagostena L, Ashmore JF, Kachar B, Mammano F (2001). Purinergic control of intercellular communication between Hensen's cells of the guinea-pig cochlea. The Journal of Physiology, 531, 693 - 706.

    2000

    • Griesinger CB, Richards CD, Ashmore J (2000). Tracing of membrane turnover in hair cells of the guinea pig organ of Corti.
    • Lagostena L, Ashmore JF, Mammano F (2000). Two Ca2+-dependent currents in Hensen's cells of the organ of Corti.. BIOPHYS J, 78(1), 12A - 12A.
    • Jagger DJ, Griesinger CB, Rivolta MN, Holley MC, Ashmore JF (2000). Calcium signalling mediated by the ?9 acetylcholine receptor in a cochlear cell line from the Immortomouse . The Journal of Physiology, 527, 49 - 54.
    • de Boer J, Ashmore JF (2000). In situ measurement of electrical coupling in Deiters' cells in the organ of Corti.
    • Ashmore JF, Geleoc GSG, Harbott L (2000). Properties of a sugar uptake system in isolated cochlear outer hair cells.. BIOPHYS J, 78(1), 51A - 51A.
    • Ashmore J, Gale J (2000). The cochlea. Current Biology, 10(9), R325 - R327.
    • Jagger DJ, Griesinger CB, Rivolta MN, Holley MC, Ashmore JF (2000). Calcium signalling mediated by the 9 acetylcholine receptor in a cochlear cell line from the immortomouse.. J Physiol, 527 Pt 1, 49 - 54.
    • Lagostena L, Ashmore JF, Mammano F (2000). Effects of ATP on intracellular Ca2+ dynamics and membrane currents in Hensen's cells of the isolated guinea-pig cochlea. J PHYSIOL-LONDON, 523, 193P - 193P.
    • Ashmore JF (2000). Hearing and the molecular mechanisms of sound amplification in the inner ear. J PHYSIOL-LONDON, 523, 15S - 15S.
    • de Boer J, Ashmore JF (2000). In situ measurement of electrical coupling of deiters' cells in the organ of corti. EUR J NEUROSCI, 12, 493 - 493.
    • Ashmore JF, Geleoc GS, Harbott L (2000). Molecular mechanisms of sound amplification in the mammalian cochlea. Proceedings of the National Academy of Sciences of the United States of America, 97(22), 11759 - 11764.
    • Ashmore JF (2000). The Physiology of Hearing. In Kruth P, Stobart HF (Ed.), Sound: The Darwin College Lectures (pp. 65 - 88). : Cambridge University Press.

    1999

    • Jagger DJ, Ashmore JF (1999). The fast activating potassium current, I(K,f), in guinea-pig inner hair cells is regulated by protein kinase A.. Neurosci Lett, 263(2-3), 145 - 148.
    • Geleoc GS, Forge A, Casalotti S, Ashmore JF (1999). A sugar transporter as a candidate for the outer hair cell motor. Nature Neuroscience, 2(8), 713 - 719.
    • de Boer J, Ashmore JF (1999). An interferometer insert for an upright microscope.
    • Richmond S, Ashmore JF (1999). Calcium buffering in turn 4 outer hair cells of the in situ cochlea.
    • Kimitsuki T, Ashmore JF (1999). Evidence for vesicle recycling in isolated inner hair cells of guinea-pig cochlea.
    • Jagger DJ, Ashmore JF (1999). Regulation of ionic currents by protein kinase A and intracellular calcium ion outer hair cells isolated from the guinea pig cochlea. Pflügers Archiv European Journal of Physiology, 437, 409 - 416.
    • Ashmore JF, Bolsover SR, Cockcroft S, Duchen MR, Koffer A, Moss SE, Swann K (1999). Imaging as applied to cell signalling: work from the Physiology Imaging Consortium (UCL.PIC).
    • Ashmore JF, de Boer J (1999). Spreading the fluid word. Nature, 402, 476 - 477.
    • Harbott LK, Geleoc GS, Ashmore JF (1999). Selectivity of sugar transport in isolated outer hair cells of the guinea-pig.
    • Jagger DJ, Holley MC, Ashmore JF (1999). Ionic currents expressed in a cell line derived from the organ of Corti of the Immortomouse. Pflügers Archiv European Journal of Physiology, 438, 8 - 14.
    • Ashmore JF, Geleoc GS (1999). Hearing in the fast lane. Current Biology, 9, 572 - 574.

    1998

    • Jagger DJ, Ashmore JF (1998). A potassium current in guinea pig outer hair cells activated by ion channel blocker DCDPC. NeuroReport, 9, 3887 - 3891.
    • Ashmore JF (1998). Swimming round in circles. Current Biology, 8, 425 - 427.
    • Geleoc GSG, Ashmore JF (1998). Sugar transport in isolated outer hair cells of the guinea-pig cochlea.
    • Nobili R, Mammano F, Ashmore JF (1998). How well do we understand the cochlea?. Trends in Neurosciences, 21, 159 - 167.
    • Geleoc GSG, Ashmore JF (1998). Interaction between sugar transport and the mechanism of fast motility in guinea-pig outer hair cells.
    • Ashmore JF (1998). Hearing. In Bock GR, Goode JA (Ed.), The Limits of Reductionism in Biology (pp. 133 - 141). : John Wiley & Sons.
    • Richmond S, Ashmore J (1998). Comparison of turn 4 and turn 1 in situ outer hair cells of the guinea-pig cochlea.
    • Jagger DJ, Grix N, Holley MC, Rivolta MN, Ashmore JF (1998). Electrophysiological evidence for the alpha-9 acetylcholine receptor in the cochlear cell line UB-OC1.
    • Jagger DJ, Rivolta MN, Holley MC, Ashmore JF (1998). Cholinergic and voltage-activated currents expressed in a cochlear cell line derived from the immortomouse.
    • Jagger DJ, Rivolta MN, Holley MC, Ashmore JF (1998). Cholinergic and voltage-activated currents expressed in a cochlear cell line derived from the Immortomouse (TM). J PHYSIOL-LONDON, 509P, 189P - 190P.
    • Geleoc GSG, Ashmore JF (1998). Sugar transport in isolated outer hair cells of the guinea-pig cochlea. J PHYSIOL-LONDON, 506P, 131P - 131P.
    • Rivolta MN, Grix N, Lawlor P, Ashmore JF, Jagger DJ, Holley MC (1998). Auditory hair cell precursors immortalised from the mammalian inner ear. Proceedings of the Royal Society B: Biological Sciences, 265(1406), 1595 - 1603.

    1997

    • Gale JE, Ashmore JF (1997). An intrinsic limit to the cochlear amplifier. Nature, 369, 63 - 66.
    • Gale JE, Ashmore JF (1997). An intrinsic frequency limit to the cochlear amplifier. NATURE, 389(6646), 63 - 66.
    • Gale JE, Ashmore JF (1997). The outer hair cell motor in membrane patches. European Journal of Physiology, 434, 267 - .
    • Rennie KJ, Ashmore JF, Correia MJ (1997). Evidence for a Na-K-Cl cotransporter in mammalian Type I hair cells. American Journal of Physiology, 273, C1972 - C1980.

    1977

    • ASHMORE JF, FALK G (1977). FLUCTUATIONS IN RETINAL BIPOLAR CELL RESPONSES TO DIM LIGHT-FLASHES. J PHYSIOL-LONDON, 269(1), P27 - P28.
    • ASHMORE JF, FALK G (1977). DARK NOISE IN RETINAL BIPOLAR CELLS AND STABILITY OF RHODOPSIN IN RODS. NATURE, 270(5632), 69 - 71.

    1976

    • ASHMORE JF, FALK G (1976). ABSOLUTE SENSITIVITY OF ROD BIPOLAR CELLS IN A DARK-ADAPTED RETINA. NATURE, 263(5574), 248 - 249.
    • ASHMORE JF, FALK G (1976). SOME PROPERTIES OF BIPOLAR CELLS IN RETINA OF DOGFISH AND RAYS. J PHYSIOL-LONDON, 258(2), P39 - P40.

    • Mistrik P, Ashmore J (). Using A Large Scale Computational Model To Study The Effect Of Longitudinal And Radial Electrical Coupling In The Cochlea.. In Cooper NP, Kemp DT (Ed.), Mechanics Of Hearing 2008: Proceedings of the 10th International Workshop (pp. - ). : World Scientific Publishing Company.