Prof Tony Segal

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Personal Profile

Name: Tony Segal Email: t.segal@ucl.ac.uk
Title: Prof Tel: 0044 20 7679 6175
Department: Internal Medicine Fax: 0044 20 7679 0967
Position: Charles Dent Professor of Medicine Address: The Rayne Building, Centre for Molecular Medicine, 5 University Street, London, WC1E 6JF
Research Domain: Basic Life Sciences, Experimental Medicine, Infection, Immunology & Inflammation, Personalised Medicine Web Page: Personal Web Page

Profile

Research Description

The primary research purpose of this group is to understand the way in which the "professional" phagocytic cells, neutrophils, monocyte/macrophages and eosinophils, kill and digest invading microbes. 1. A central interest is the NADPH oxidase, a system responsible for the generation of superoxide and other oxygen radicals. This work involves investigation of: the flavocytochrome b electron transport chain; signal transduction mechanisms responsible for activation of superoxide production; interaction of components of the NADPH oxidase with the cytoskeleton. 2. The inflammatory bowel diseases, Crohn's disease and ulcerative colitis, are chronic inflammatory conditions of unknown cause which primarily affect the gastrointestinal tract. We have shown that, counter-intuitively, the acute inflammatory response and neutrophil accumulation are defective in Crohn's disease. Identification of this major phenotypic abnormality provides a significant breakthrough in understanding the pathological events that cause Crohn's disease. We are investigating how this might predispose to chronic bowel inflammation through delayed clearance of bowel bacteria penetrating the mucosa using the following approaches: injection of 32P-labelled, heat - killed E. coli into the subcutaneous tissues of the forearm, to measure clearance as the removal of radioactivity; microarray analysis of global gene transcription profiles of monocyte/macrophages; quantitation of secretion of pro-inflammatory cytokines from monocyte/macrophages. In line with the genetic evidence in the literature, preliminary studies in ulcerative colitis have identified a completely different abnormality in which the resolution of acute inflammation is delayed; these findings are currently under investigation.

Research Activities

Bioenergetics

Cell Biology of Phagocytes

DNA replication licensing and cell proliferation

Inflammatory Bowel Diseases

Molecular Basis of Inflammatory Bowel Diseases

Proteomics of Signal Transduction Pathways

Regulation and targeting of the hypoxia inducible factor (HIF) pathway

Understaning the cellular players and soluble medi

Education Description

Director of the UCL MBPhD programme

UCL Collaborators

Prof Barbara Pedley; Prof David Gems; Dr Geoffrey Bellingan; Prof Derek Gilroy; Prof David Selwood; Prof Gareth Hayden Williams; Prof Patrick Maxwell; Prof Michael Duchen; Prof Mervyn Singer; Prof Arne Akbar; Prof Margaret Ashcroft; Prof Peter Rich; Prof Steve Wilson; Prof Mike Ehrenstein; Prof Martin Widschwendter; Prof Jasminka Godovac-Zimmermann; Prof Chris Boshoff; Prof Stephen Caddick; Dr Stuart Bloom; Dr Andrei Okorokov

External Collaborators

Dr Rebeca Perez De Diego; Prof Jean-Laurent Casanova; Prof Marko Radulovic; Dr Gariella Pinto; Prof Laurent Abel; Dr Matthew Wb Trotter

Publications

    2013

    • Chen Y, Ogundare M, Williams CM, Wang Y, Wang Y, Sewell GW, Smith PJ, Rahman FZ, O'Shea N, Segal AW, Griffiths WJ (2013). Shotgun cholanomics of ileal fluid. BIOCHIMIE, 95(3), 461 - 463. doi:10.1016/j.biochi.2012.09.004

    2012

    • O'shea NR, Chew TS, Sewell G, Bloom S, Smith A, Segal A (2012). ADAMDEC1: A NOVEL MOLECULE IN INFLAMMATION AND BOWEL DISEASE. GUT, 61, A392 - A392. doi:10.1136/gutjnl-2012-302514d.232
    • Sewell GW, Hannun YA, Han X, Koster G, Bielawski J, Goss V, Smith PJ, Rahman FZ, Vega R, Bloom SL, Walker AP, Postle AD, Segal AW (2012). Lipidomic profiling in Crohn's disease: abnormalities in phosphatidylinositols, with preservation of ceramide, phosphatidylcholine and phosphatidylserine composition.. Int J Biochem Cell Biol, 44(11), 1839 - 1846. doi:10.1016/j.biocel.2012.06.016
    • Smith PJ, Levine AP, Sewell GW, O'Shea NR, Vega R, Bloom SL, Smith AM, Segal AW (2012). MUCOSAL MRNA EXPRESSION PROFILING FROM THE TERMINAL ILEUM AND COLON REVEALS UNDER EXPRESSION OF CLAUDIN 8, A TIGHT JUNCTION MOLECULE, AS POTENTIALLY CAUSAL IN ULCERATIVE COLITIS. GUT, 61, A238 - A239. doi:10.1136/gutjnl-2012-302514c.130
    • Sewell GW, Rahman FZ, Levine AP, Jostins L, Smith PJ, Walker AP, Bloom SL, Segal AW, Smith AM (2012). Defective tumor necrosis factor release from Crohn's disease macrophages in response to Toll-like receptor activation: relationship to phenotype and genome-wide association susceptibility loci.. Inflamm Bowel Dis, 18(11), 2120 - 2127. doi:10.1002/ibd.22952
    • Rodriguez-Gallego JC, Ouederni M, Boisson-Dupuis S, Puel A, Picard C, Sologuren I, Bustamante J, Samarina A, Herrera-Ramos E, Lopez-Rodriguez M, Dogu F, Tanir G, Ikinciogullari A, Sanal O, Pedraza S, Colino E, Keser M, Nieuwhof C, Kumararatne D, Segal A, Levy J, Kutukculer N, Karaca N, Barbouche R, Bejaoui M, Kachboura S, Lezana-Fernandez JL, Bobadilla-Del Valle M, Aytekin C, Abel L, Casanova JL (2012). CLINICAL FEATURES OF CANDIDIASIS IN PATIENTS WITH INTERLEUKIN-12 RECEPTOR B1 DEFICIENCY.
    • Smith BN, Evans C, Ali A, Ancliff PJ, Hayee B, Segal AW, Hall G, Kaya Z, Shakoori AR, Linch DC, Gale RE (2012). Phenotypic heterogeneity and evidence of a founder effect associated with G6PC3 mutations in patients with severe congenital neutropenia.. Br J Haematol, 158(1), 146 - 149. doi:10.1111/j.1365-2141.2012.09110.x

    2011

    • Hayee B, Antonopoulos A, Murphy EJ, Rahman FZ, Sewell G, Smith BN, McCartney S, Furman M, Hall G, Bloom SL, Haslam SM, Morris HR, Boztug K, Klein C, Winchester B, Pick E, Linch DC, Gale RE, Smith AM, Dell A, Segal AW (2011). G6PC3 mutations are associated with a major defect of glycosylation: a novel mechanism for neutrophil dysfunction.. Glycobiology, 21(7), 914 - 924. doi:10.1093/glycob/cwr023
    • Hayee B, Rahman FZ, Tempero J, McCartney S, Bloom SL, Segal AW, Smith AM (2011). The neutrophil respiratory burst and bacterial digestion in Crohn's disease.. Dig Dis Sci, 56(5), 1482 - 1488. doi:10.1007/s10620-010-1426-8
    • Thrasher AJ, Segal AW (2011). A phagocyte dilemma.... Nat Immunol, 12(3), 201 - 202. doi:10.1038/ni0311-201

    2010

    • Murphy EJ, Maréchal A, Segal AW, Rich PR (2010). CO binding and ligand discrimination in human myeloperoxidase.. Biochemistry, 49(10), 2150 - 2158. doi:10.1021/bi9021507
    • Glocker EO, Schaffer AA, Perro M, Allroth A, Sykora KW, Sauer M, Kreipe H, Nustede R, Woellner C, Baumann U, Salzer U, Segal AW, Klein C, Grimbacher B (2010). Severe early onset ileo-colitis can be caused by mutations in IL10RB and successfully treated by allogeneic hematopoietic stem cell transplantation.
    • Segal A, Rahman FZ, Smith AM, Hayee B, Marks DJ, Bloom SL (2010). Delayed resolution of acute inflammation in ulcerative colitis is associated with elevated cytokine release downstream of TLR4.. Expert Review of Clinical Immunology, 6(4), 585 - 596.
    • Hayee B, Sewell G, Rahman FZ, Bloom SL, Smith AM, Segal AW (2010). DIFFERENTIAL BACTERIAL CLEARANCE AND CYTOKINE SECRETION BY MACROPHAGES EXPLAINS LOCALISATION OF CROHN'S DISEASE TO THE GUT. doi:10.1136/gut.2009.209031r
    • Shao D, Segal AW, Dekker LV (2010). Subcellular localisation of the p40phox component of NADPH oxidase involves direct interactions between the Phox homology domain and F-actin.. Int J Biochem Cell Biol, 42(10), 1736 - 1743. doi:10.1016/j.biocel.2010.07.009
    • Rahman FZ, Smith AM, Hayee B, Marks DJ, Bloom SL, Segal AW (2010). Delayed resolution of acute inflammation in ulcerative colitis is associated with elevated cytokine release downstream of TLR4.. PLoS One, 5(3), e9891 - . doi:10.1371/journal.pone.0009891
    • Segal AW (2010). Crohn's is an immunodeficiency disease.
    • Segal A, Murphy EJ, Marechal A, Rich PR (2010). CO binding and ligand discrimination in human myeloperoxidase.. Biochemistry, 49(10), 2150 - 2158.
    • Segal AW (2010). Studies to identify the causal molecules in Crohn's disease.
    • Hayee B, Rahman FZ, Sewell G, Smith AM, Segal AW (2010). Crohn's disease as an immunodeficiency.. Expert Rev Clin Immunol, 6(4), 585 - 596. doi:10.1586/eci.10.32

    2009

    • Smith N, Hayee B, Evans C, Ancliff P, Hall G, Segal A, Linch D, Gale R (2009). NOVEL G6PC3 MUTATIONS IDENTIFIED IN AUTOSOMAL RECESSIVE SCN PATIENTS WITH AND WITHOUT CONGENITAL ABNORMALITIES.
    • Marks DJ, Rahman FZ, Sewell GW, Segal AW (2009). Crohn's disease: an immune deficiency state.. Clinical Reviews in Allergy and Immunology, 38(1), 20 - 31. doi:10.1007/s12016-009-8133-2
    • Sewell GW, Marks DJ, Segal AW (2009). The immunopathogenesis of Crohn's disease: a three-stage model.. Curr Opin Immunol, 21(5), 506 - 513. doi:10.1016/j.coi.2009.06.003
    • Rahman FZ, Hayee B, Chee R, Segal AW, Smith AM (2009). Impaired macrophage function following bacterial stimulation in chronic granulomatous disease.. Immunology, 128(2), 253 - 259. doi:10.1111/j.1365-2567.2009.03112.x
    • Rahman FZ, Haye B, Chee R, Segal AW, Smith AM (2009). Impaired macrophage function following bacterial stimulation in chronic granulomatous disease.. Immunology, 128(2), 253 - 259. doi:10.1111/j.1365-2567.2009.03112.x
    • Palmer CD, Rahman FZ, Sewell GW, Ahmed A, Ashcroft M, Bloom SL, Segal AW, Smith AM (2009). Diminished macrophage apoptosis and reactive oxygen species generation after phorbol ester stimulation in Crohn's disease.. PLoS One, 4(11), e7787 - . doi:10.1371/journal.pone.0007787
    • Glocker EO, Kotlarz D, Boztug K, Gertz EM, Schaffer AA, Noyan F, Perro M, Diestelhorst J, Allroth A, Murugan D, Hatscher N, Pfeifer D, Sykora KW, Sauer M, Kreipe H, Lacher M, Nustede R, Woellner C, Baumann U, Salzer U, Koletzko S, Shah N, Segal AW, Sauerbrey A, Buderus S, Snapper SB, Grimbacher B, Klein C (2009). Inflammatory Bowel Disease and Mutations Affecting the Interleukin-10 Receptor. New England Journal of Medicine, 361(21), 2033 - 2045. doi:10.1056/NEJMe0909225
    • Smith AM, RahmanF Z H, B G, S J M, D J S, G W P, C D W, J F, B M G, I S S, T J M, M W, A P B, S L S, A W (2009). Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.. Journal of Experimental Medicine, 206(9), 1883 - 1897.
    • Smith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW (2009). Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.. J Exp Med, 206(9), 1883 - 1897. doi:10.1084/jem.20091233
    • Glocker EO, Kotlarz D, Boztug K, Gertz EM, Schäffer AA, Noyan F, Perro M, Diestelhorst J, Allroth A, Murugan D, Hätscher N, Pfeifer D, Sykora KW, Sauer M, Kreipe H, Lacher M, Nustede R, Woellner C, Baumann U, Salzer U, Koletzko S, Shah N, Segal AW, Sauerbrey A, Buderus S, Snapper SB, Grimbacher B, Klein C (2009). Inflammatory bowel disease and mutations affecting the interleukin-10 receptor.. N Engl J Med, 361(21), 2033 - 2045. doi:10.1056/NEJMoa0907206
    • Boztug K, Kotlarz D, Glocker E, Gertz ME, Schaffer AA, Noyan F, Perro M, Diestelhorst J, Allroth A, Murugan D, Hatscher N, Pfeifer D, Sykora KW, Sauer M, Kreipe H, Lacher M, Nustede R, Woellner C, Baumann U, Salzer U, Koletzko S, Shah N, Segal A, Sauerbrey A, Buderus S, Snapper S, Grimbacher B, Klein C (2009). Severe Early-Onset Inflammatory Bowel Disease Caused by IL10 Receptor Deficiency Can Be Cured by Allogeneic Hematopoietic Stem Cell Transplantation.
    • Glocker E, Kotlarz D, Boztug K, Gertz E, Schaffer A, Koletzko S, Shah N, Segal A, Snapper S, Grimbacher B, Klein C (2009). Early-onset inflammatory bowel disease caused by loss-of-function mutations in the IL10-receptor genes.
    • Xu P, Way M, Godovac-Zimmermann JK, O S, A W and Radulovic M (2009). Subproteome analysis of the neutrophil cytoskeleton. Proteomics, 9, 2037 - 2049.
    • Rahman FZ, Smith AM, Hayee B, Sewell G, Marks DJ, Bloom SL, Segal AW (2009). ABNORMAL TLR4-MEDIATED INTERFERON RESPONSE IN ULCERATIVE COLITIS.
    • Hayee B, Rahman FZ, Smith AM, Marks DJB, Sewell GW, Sweeting TJ, Bloom SL, Segal AW (2009). BACTERIAL CLEARANCE IS DELAYED IN PATIENTS WITH CROHN'S DISEASE.
    • Marks DJ, Miyagi K, Rahman FZ, Novelli M, Bloom SL, Segal AW (2009). Inflammatory bowel disease in CGD reproduces the clinicopathological features of Crohn's disease.. , 104(1), 117 - 124. doi:10.1038/ajg.2008.72

    2008

    • Hayee B, Rahman FZ, Tempero J, Marks DJB, Smith AM, Bloom SL, Segal AW (2008). The prevalence of undiagnosed disorders of neutrophil function mimicking crohn's disease.
    • Rahman FZ, Marks DJ, Hayee BH, Smith AM, Bloom SL, Segal AW (2008). Phagocyte dysfunction and inflammatory bowel disease.. Inflammatory Bowel Diseases, 14(10), 1443 - 1452. doi:10.1002/ibd.20449
    • Segal AW (2008). The function of the NADPH oxidase of phagocytes and its relationship to other NOXs in plants, invertebrates, and mammals.. Int J Biochem Cell Biol, 40(4), 604 - 618. doi:10.1016/j.biocel.2007.10.003
    • Marks DJ, Segal AW (2008). Innate immunity in inflammatory bowel disease: a disease hypothesis.. Journal of Pathology, 214(2), 260 - 266. doi:10.1002/path.2291
    • Rahman FZ, Marks DJ, Hayee BH, Smith AM, Bloom SL, Segal AW (2008). Phagocyte dysfunction and inflammatory bowel disease.. Inflamm Bowel Dis, 14(10), 1443 - 1452. doi:10.1002/ibd.20449

    2007

    • Marks DJ, Radulovic M, McCartney S, Bloom S, Segal AW (2007). Modified skin window technique for the extended characterisation of acute inflammation in humans.. Inflammation Research, 56(4), 168 - 174. doi:10.1007/s00011-006-6119-6
    • Behe P, Segal AW (2007). The function of the NADPH oxidase of phagocytes, and its relationship to other NOXs.. Biochem Soc Trans., 35(5), 1100 - 1103. doi:10.1042/BST0351100
    • Chua F, Dunsmore SE, Clingen PH, Mutsaers SE, Shapiro SD, Segal AW, Roes J, Laurent GJ (2007). Mice lacking neutrophil elastase are resistant to bleomycin-induced pulmonary fibrosis. American Journal of Pathology, 170(1), 65 - 74. doi:10.2353/ajpath.2007.060352

    2006

    • Xu P, Roes J, Segal AW, Radulovic M (2006). The role of grancalcin in adhesion of neutrophils.. Cellular Immunology, 240(2), 116 - 121. doi:10.1016/j.cellimm.2006.07.004
    • Marks DJ, Mitchison NA, Segal AW, Sieper J (2006). Can unresolved infection precipitate autoimmune disease?. Current Topics in Microbiology and Immunology, 305, 105 - 125.
    • Marks DJ, Harbord MW, MacAllister R, Rahman FZ, Young J, Al-Lazikani B, Lees W, Novelli MR, Bllom S, Segal AW (2006). Defective acute inflammation in Crohn's disease: a clinical investigation.. The Lancet, 367(9511), 668 - 678. doi:10.1016/S0140-6736(06)68265-2
    • Segal AW (2006). How superoxide production by neutrophil leukocytes kills microbes.. Novartis Found Symp., 279, 92 - 98.
    • Marks DJ, Rahman FZ, Novelli M, Yu RC, McCartney S, Bloom S, Segal AW (2006). An exuberant inflammatory response to E coli: implications for the pathogenesis of ulcerative colitis and pyoderma gangrenosum.. Gut, 55(11), 1662 - 1663. doi:10.1136/gut.2006.104943
    • Harbord MW, Marks DJ, Forbes A, Bloom SL, Day RM, Segal AW (2006). Impaired neutrophil chemotaxis in Crohn's disease relates to reduced production of chemokines and can be augmented by granulocyte-colony stimulating factor. Aliment.Pharmacol.Ther., 24(4), 651 - 660.
    • Singh S, Marks DJB, Rahman FZ, Bloom S, Yu R, Segal AW (2006). Experimentally-induced pyoderma gangrenosum.

    2005

    • Segal AW (2005). How neutrophils kill microbes.. Annual Review of Immunology, 23, 197 - 223.
    • Marks D, Oukrif D, Novelli M, Forbes A, Segal AW, Harbord M (2005). Impaired acute inflammation in the terminal ileum of Crohn's patients.

    2004

    • Marks DJB, Harbord M, Al-Lazikani B, Joshi M, Bloom S, Segal AW (2004). Characterisation of the defect in acute inflammation in Crohn's disease and the influence of CARD15.
    • Ahluwalia J, Tinker A, Clapp LH, Duchen MR, Abramov AY, Pope S, Nobles M, Segal AW (2004). The large-conductance Ca2+-activated K channel is essential for innate immunity.. Nature, 427(6977), 853 - 858. doi:10.1038/nature02356
    • Paclet MH, Davis C, Kotsonis P, Godovac-Zimmermann J, Segal AW, Dekker LV (2004). N-Formyl peptide receptor subtypes in human neutrophils activate L-plastin phosphorylation through different signal transduction intermediates.. Biochemical Journal, 377(2), 469 - 477.
    • Tinker A, Clapp L, Segal A (2004). An essential role for BKCa channels in innate immunity.
    • Cross AR, Segal AW (2004). The NADPH oxidase of professional phagocytes--prototype of the NOX electron transport chain systems.. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1657(1), 1 - 22.
    • Harbord M, Marks DJB, Oukrif D, Novelli M, Segal AW, Bloom S (2004). A novel method to investigate the inflammatory response in the bowel, and confirmation of its impairment in Crohn's disease.

    2003

    • Bird MM, Lopez-Lluch G, Ridley AJ, Segal AW (2003). Effects of microinjected small GTPases on the actin cytoskeleton of human neutrophils. Journal of Anatomy, 203(4), 379 - 389.
    • Shao D, Segal AW, Dekker LV (2003). Lipid rafts determine efficiency of NADPH oxidase activation in neutrophils. FEBS Letters, 550(1-3), 101 - 106.
    • Reeves EP, Nagl M, Godovac-Zimmermann J, Segal AW (2003). Reassessment of the microbicidal activity of reactive oxygen species and hypochlorous acid with reference to the phagocytic vacuole of the neutrophil granulocyte. Journal of Medical Microbiology, 52(Pt 8), 643 - 651.
    • Roes J, Choi BK, Power D, Xu P, Segal AW (2003). Granulocyte function in grancalcin-deficient mice. Molecular and Cellular Biology, 23(3), 826 - 830. doi:10.1128/MCB.23.3.826-830.2003
    • Wientjes FB, Segal AW (2003). PX domain takes shape. Current Opinion in Hematology, 10(1), 2 - 7.

    2002

    • Harbord M, Novelli M, Canas B, Power D, Davis C, Godovac-Zimmermann J, Roes J, Segal AW (2002). Ym1 is a neutrophil granule protein that crystallizes in p47phox-deficient mice. Journal of Biological Chemistry, 277(7), 5468 - 5475. doi:10.1074/jbc.M110635200
    • Bird M, Segal A (2002). Microinjected neutrophils retain the ability to take up bacteria.. Journal of Anatomy, 200(5), 534 - 534.
    • Reeves EP, Lu H, Jacobs HL, Messina CGM, Bolsover S, Gabella G, Potma EO, Warley A, Roes J, Segal AW (2002). Killing activity of neutrophils is mediated through activation of proteases by K+ flux. Nature, 416(6878), 291 - 297. doi:10.1038/416291a
    • Harbord M, Day R, Hankin A, Bloom S, Forbes A, Segal AW (2002). Neutrophil respiratory burst and tissue penetration are normal in CD during G-CSF administration. GUT, 50, A67 - A67.
    • Messina CG, Reeves EP, Roes J, Segal AW (2002). Catalase negative Staphylococcus aureus retain virulence in mouse model of chronic granulomatous disease.. FEBS Lett, 518(1-3), 107 - 110.
    • Davidson-Moncada JK, Lopez-Lluch G, Segal AW, Dekker LV (2002). Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochemical Journal, 363(Pt 1), 95 - 103.
    • Chua F, Dunsmore SE, Segal AW, Roes J, Laurent GJ (2002). TGF-beta activation is diminished following bleomycin-induced lung injury in mice lacking neutrophil elastase.
    • Day R, Harbord M, Segal A, Forbes A (2002). Differences in micro-vascular blood flow at sites of skin blistering may account for reduced leukocyte trafficking in Crohn's disease. GUT, 50, A77 - A77.

    2001

    • Lopez-Luch G, Bird MM, Canas B, Godovac-Zimmermann J, Ridley A, Segal AW, Dekker LV (2001). The PKC-d C2-like domain is a binding site for actin and enables PKC-d-induced actin remodelling in neutrophils.. Biochemical Journal, 357, 39 - 47.
    • Lopez-Lluch G, Bird MM, Canas B, Godovac-Zimmerman J, Ridley A, Segal AW, Dekker LV (2001). Protein kinase C-delta C2-like domain is a binding site for actin and enables actin redistribution in neutrophils.. Biochemical Journal, , - .
    • Lopez-Lluch G, Bird WM, Canas B, Godovac-Zimmerman J, Ridley A, Segal AW, Dekker LV (2001). Protein kinase C-delta C2-like domain is a binding site for actin and enables actin redistribution in neutrophils. BIOCHEM J, 357, 39 - 47.
    • Dunsmore SE, Roes J, Chua FJ, Segal AW, Mutsaers SE (2001). Evidence that neutrophil elastase-deficient mice are resistant to bleomycin-induced fibrosis.. Chest, , - .
    • Day RM, Harbord M, Forbes A, Segal AW (2001). Cantharidin blisters: a technique for investigating leukocyte trafficking and cytokine production at sites of inflammation in humans. Journal of Immunological Methods, 257(1-2), 213 - 220. doi:10.1016/S0022-1759(01)00467-7
    • Wientjes FB, Reeves EP, Soskic V, Furthmayr H, Segal AW (2001). The NADPH oxidase components p47(phox) and p40(phox) bind to moesin through their PX domain. Biochemical and Biophysical Research Communications, 289(2), 382 - 388.
    • Dunsmore SE, Roes J, Chua FJ, Segal AW, Mutsaers SE, Laurent GJ (2001). Evidence that neutrophil elastase-deficient mice are resistant to bleomycin-induced fibrosis.. Chest, 120(1 Suppl), 35S - 36S.
    • Harbord M, Day R, Bloom S, Knight S, Forbes A, Segal AW (2001). Neutrophil deficiency during acute inflammation in inflammatory bowel disease. GUT, 48, A20 - A20.
    • Chua F, Dunsmore S, Segal AW, Roes J, Laurent GJ (2001). Alveolar-capillary leak is attenuated in neutrophil elastase 'knockout' mice with bleomycin-induced lung injury. THORAX, 56, 5 - 6.

    2000

    • Tkalcevic J, Novelli M, Phylactides M, Iredale JP, Segal AW, Roes J (2000). Impaired immunity and enhanced resistance to endotoxin in the absence of neutrophil elastase and cathepsin G.. Immunity, 12(2), 201 - 210.
    • Didichenko SA, Segal AW, Thelen M (2000). Evidence for a pool of coronin in mammalian cells that is sensitive to PI 3-kinase. FEBS Letters, 485(2-3), 147 - 152.
    • Chua F, Dunsmore SE, Segal AW, Roes J, Laurent GJ (2000). Bleomycin-induced pulmonary fibrosis is attenuated in neutrophil elastase-deficient mice. THORAX, 55, A14 - A14.
    • Dekker LV, Segal AW (2000). Signal transduction - Signals to move cells. SCIENCE, 287(5455), 982 - +.
    • Dekker LV, Segal AW (2000). Perspectives: signal transduction. Signals to move cells. Science, 287(5455), 982 - 3, 985.
    • Dekker LV, Leitges M, Altschuler G, Mistry N, McDermott A, Roes J, Segal AW (2000). Protein kinase C-beta contributes to NADPH oxidase activation in neutrophils. Biochemical Journal, 347 Pt, 285 - 289.

    1999

    • Reeves EP, Dekker LV, Forbes LV, Wientjes FB, Grogan A, Pappin DJ, Segal AW (1999). Direct interaction between p47phox and protein kinase C: evidence for targeting of protein kinase C by p47phox in neutrophils.. Biochem J, 344 Pt 3, 859 - 866.
    • Leino L, Forbes L, Segal A, Cockcroft S (1999). Reconstitution of GTPgammaS-induced NADPH oxidase activity in streptolysin-O permeabilised neutrophils by specific cytosol fractions. Biochemical and Biophysical Research Communications, 265, 29 - 37.
    • Forbes LV, Moss SJ, Segal AW (1999). Phosphorylation of p67phox in the neutrophil occurs in the cytosol and is independent of p47phox. Federation of European Biochemical Societies Letters, 449, 225 - 229.
    • Reeves EP, Dekker LV, Forbes LV, Wientjes FB, Grogan A, Pappin DJ, Segal AW (1999). Direct interaction between p47phox and protein kinase C: evidence for targeting of protein kinase C by p47phox in neutrophils. Biochemical Journal, 344 Pt, 859 - 866.
    • Forbes LV, Truong O, Wientjes FB, Moss SJ, Segal AW (1999). The major phosphorylation site of the NADPH oxidase component p67phox is Thr233.. Biochem J, 338 ( Pt 1), 99 - 105.
    • Lal AS, Clifton AD, Rouse J, Segal AW, Cohen P (1999). Activation of the neutrophil NADPH oxidase is inhibited by SB 203580, a specific inhibitor of SAPK2/p38. Biochemical and Biophysical Research Communications, 259(2), 465 - 470.
    • Lal AS, Parker PJ, Segal AW (1999). Characterization and partial purification of a novel neutrophil membrane-associated kinase capable of phosphorylating the respiratory burst component p47phox. Biochemical Journal, 338(Pt 2), 359 - 366.

    1998

    • M'rabet L, Coffer P, Zwartkruis F, Franke B, Segal A, Koenderman L, Bos J (1998). Activation of the small GTPase Rap1 in human neutrophils. J LEUKOCYTE BIOL, , 17 - 17.
    • M'Rabet L, Coffer P, Zwartkruis F, Franke B, Segal AW, Koenderman L, Bos JL (1998). Activation of the small GTPase rap1 in human neutrophils. BLOOD, 92(6), 2133 - 2140.
    • Lammas DA, Altare F, Revy P, Darbyshire P, Segal A, Casanova JL, Kumararatne DS (1998). Novel Human Immunodeficiencies resulting in increased susceptibility to mycobacterial and salmonella infections.. MOL IMMUNOL, 35(11-12), 729 - 729.
    • David V, Gouin E, Van Troys M, Grogan A, Segal AW, Ampe C, Cossart P (1998). Identification of cofilin, coronin, Rac and capZ in actin tails using a Listeria affinity approach. J CELL SCI, 111, 2877 - 2884.
    • Altare F, Durandy A, Lammas D, Emile JF, Lamhamedi S, Le Deist F, Drysdale P, Jouanguy E, Doffinger R, Bernaudin F, Jeppsson O, Gollob JA, Meinl E, Segal AW, Fischer A, Kumararatne D, Casanova JL (1998). Impairment of mycobacterial immunity in human interleukin-12 receptor deficiency. SCIENCE, 280(5368), 1432 - 1435.
    • Kumararatne DS, Drysdale P, Lammas DA, Quibell K, Schoel-Tollner D, Girdlestone J, Wadhwa M, Dilger P, Dockrell H, Britten K, Segal A (1998). Studies on two patients with disseminated intracellular mycobacterial infections, one with defective IL-12 p40 production and the other with defective IL-12 receptor signalling.. J ALLERGY CLIN IMMUN, 101(1), S55 - S55.

    1997

    • Segal AW, Shatwell KP (1997). The NADPH oxidase of phagocytic leukocytes.. Ann N Y Acad Sci, 832, 215 - 222.
    • Forbes LV, Truong O, Moss SJ, Segal AW (1997). Identification of the major phosphorylation site of the NADPH oxidase component p67phox.. BLOOD, 90(10), 1825 - 1825.
    • Roberts GCK, Keep NH, Barnes M, Barsukov I, Badii R, Lian LY, Segal AW, Moody PCE (1997). The structure of rho-GDI and its interaction with the small GTPase RAC. FASEB J, 11(9), A1442 - A1442.
    • Wallach TM, Segal AW (1997). Analysis of glycosylation sites on gp91phox, the flavocytochrome of the NADPH oxidase, by site-directed mutagenesis and translation in vitro. BIOCHEM J, 321(Pt 3), 583 - 585.
    • (1997). The mammalian actin-related protein 2/3 complex localises to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins. Biochemical Journal, 328, 105 - 112.
    • Grogan A, Reeves E, Keep N, Wientjes F, Totty NF, Burlingame AL, Hsuan JJ, Segal AW (1997). Cytosolic phox proteins interact with and regulate the assembly of coronin in neutrophils.. Journal of Cell Science, 110, 3071 - 3081.
    • Zicha D, Dunn GA, Segal AW (1997). Deficiency of p67phox, p47phox or gp91phox in chronic granulomatous disease does not impair leucocyte chemotaxis or motility. BR J HAEMATOL, 96(3), 543 - 550.
    • Wientjes FB, Segal AW, Hartwig JH (1997). Immunoelectron microscopy shows a clustered distribution of NADPH oxidase components in the human neutrophil plasma membrane. J LEUKOC BIOL, 61(3), 303 - 312.
    • Machesky LM, Reeves E, Wientjes F, Mattheyse FJ, Grogan A, Totty NF, Burlingame AL, Hsuan JJ, Segal AW (1997). Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins. BIOCHEM J, 328(Pt 1), 105 - 112.
    • Keep NH, Barnes M, Barsukov I, Badii R, Lian LY, Segal AW, Moody PC, Roberts GC (1997). A modulator of rho family G proteins, rhoGDI, binds these G proteins via an immunoglobulin-like domain and a flexible N-terminal arm. STRUCTURE, 5(5), 623 - 633.

    1996

    • Segal AW (1996). The NADPH oxidase and chronic granulomatous disease.. Mol Med Today, 2(3), 129 - 135.
    • Roos D, deBoer M, Kuribayashi F, Meischl C, Weening RS, Segal AW, Ahlin A, Nemet K, Hossle JP, BernatowskaMatuszkiewicz E, MiddletonPrice H (1996). Mutations in the X-linked and autosomal recessive forms of chronic granulomatous disease. BLOOD, 87(5), 1663 - 1681.
    • Wallach TM, Segal AW (1996). Stoichiometry of the subunits of flavocytochrome b558 of the NADPH oxidase of phagocytes.. Biochem J, 320 ( Pt 1), 33 - 38.
    • Shatwell KP, Segal AW (1996). NADPH oxidase.. Int J Biochem Cell Biol, 28(11), 1191 - 1195.
    • Wientjes FB, Panayotou G, Reeves E, Segal AW (1996). Interactions between cytosolic components of the NADPH oxidase: p40phox interacts with both p67phox and p47phox.. Biochem J, 317 ( Pt 3), 919 - 924.
    • Shatwell KP, Dancis A, Cross AR, Klausner RD, Segal AW (1996). The FRE1 ferric reductase of Saccharomyces cerevisiae is a cytochrome b similar to that of NADPH oxidase.. J Biol Chem, 271(24), 14240 - 14244.

    1995

    • Thrasher AJ, de Alwis M, Casimir CM, Kinnon C, Page K, Lebkowski J, Segal AW, Levinsky RJ (1995). Generation of recombinant adeno-associated virus (rAAV) from an adenoviral vector and functional reconstitution of the NADPH-oxidase.. Gene Ther, 2(7), 481 - 485.
    • Wientjes FB, Segal AW (1995). NADPH oxidase and the respiratory burst.. Semin Cell Biol, 6(6), 357 - 365.
    • THRASHER AJ, DEALWIS M, CASIMIR CM, KINNON C, PAGE K, LEBKOWSKI J, SEGAL AW, LEVINSKY RJ (1995). FUNCTIONAL RECONSTITUTION OF THE NADPH-OXIDASE BY ADENOASSOCIATED VIRUS GENE-TRANSFER. BLOOD, 86(2), 761 - 765.
    • THRASHER AJ, CASIMIR CM, KINNON C, MORGAN G, SEGAL AW, LEVINSKY RJ (1995). GENE-TRANSFER TO PRIMARY CHRONIC GRANULOMATOUS-DISEASE MONOCYTES. LANCET, 346(8967), 92 - 93.
    • LOLLIKE K, SORENSEN O, BUNDGAARD JR, SEGAL AW, BOYHAN A, BORREGAARD N (1995). AN ELISA FOR GRANCALCIN, A NOVEL CYTOSOLIC CALCIUM-BINDING PROTEIN PRESENT IN LEUKOCYTES. J IMMUNOL METHODS, 185(1), 1 - 8.
    • Bu-Ghanim HN, Segal AW, Keep NH, Casimir CM (1995). Molecular analysis in three cases of X91- variant chronic granulomatous disease.. Blood, 86(9), 3575 - 3582.

    1994

    • Diekmann D, Abo A, Johnston C, Segal AW, Hall A (1994). Interaction of Rac with p67phox and regulation of phagocytic NADPH oxidase activity.. Science, 265(5171), 531 - 533.
    • Abo A, Webb MR, Grogan A, Segal AW (1994). Activation of NADPH oxidase involves the dissociation of p21rac from its inhibitory GDP/GTP exchange protein (rhoGDI) followed by its translocation to the plasma membrane.. Biochem J, 298 Pt 3, 585 - 591.
    • Thrasher AJ, Keep NH, Wientjes F, Segal AW (1994). Chronic granulomatous disease.. Biochim Biophys Acta, 1227(1-2), 1 - 24.

    1993

    • Thrasher A, Segal A, Casimir C (1993). Chronic granulomatous disease: towards gene therapy.. Immunodeficiency, 4(1-4), 327 - 333.
    • Moodie FD, Leaker B, Cambridge G, Totty NF, Segal AW (1993). Alpha-enolase: a novel cytosolic autoantigen in ANCA positive vasculitis.. Kidney Int, 43(3), 675 - 681.
    • Segal AW, Abo A (1993). The biochemical basis of the NADPH oxidase of phagocytes.. Trends Biochem Sci, 18(2), 43 - 47.
    • Segal AW (1993). Structure of the NADPH-oxidase: membrane components.. Immunodeficiency, 4(1-4), 167 - 179.

    1992

    • Thrasher A, Chetty M, Casimir C, Segal AW (1992). Restoration of superoxide generation to a chronic granulomatous disease-derived B-cell line by retrovirus mediated gene transfer.. Blood, 80(5), 1125 - 1129.
    • Boyhan A, Casimir CM, French JK, Teahan CG, Segal AW (1992). Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes.. J Biol Chem, 267(5), 2928 - 2933.
    • Segal AW (1992). Biochemistry and molecular biology of chronic granulomatous disease.. J Inherit Metab Dis, 15(4), 683 - 686.
    • Abo A, Boyhan A, West I, Thrasher AJ, Segal AW (1992). Reconstitution of neutrophil NADPH oxidase activity in the cell-free system by four components: p67-phox, p47-phox, p21rac1, and cytochrome b-245.. J Biol Chem, 267(24), 16767 - 16770.
    • Garcia RC, Whitaker M, Heyworth PG, Segal AW (1992). Okadaic acid produces changes in phosphorylation and translocation of proteins and in intracellular calcium in human neutrophils. Relationship with the activation of the NADPH oxidase by different stimuli.. Biochem J, 286 ( Pt 3), 687 - 692.
    • Teahan CG, Totty NF, Segal AW (1992). Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils.. Biochem J, 286 ( Pt 2), 549 - 554.
    • Segal AW, West I, Wientjes F, Nugent JH, Chavan AJ, Haley B, Garcia RC, Rosen H, Scrace G (1992). Cytochrome b-245 is a flavocytochrome containing FAD and the NADPH-binding site of the microbicidal oxidase of phagocytes.. Biochem J, 284 ( Pt 3), 781 - 788.

    1991

    • Segal AW (1991). Components of the microbicidal oxidase of phagocytes.. Biochem Soc Trans, 19(1), 49 - 50.
    • Casimir CM, Bu-Ghanim HN, Rodaway AR, Bentley DL, Rowe P, Segal AW (1991). Autosomal recessive chronic granulomatous disease caused by deletion at a dinucleotide repeat.. Proc Natl Acad Sci U S A, 88(7), 2753 - 2757.

    1990

    • Leoni P, Heyworth P, Segal AW (1990). Separation of phosphoproteins by fast protein liquid chromatography.. J Chromatogr, 527(1), 152 - 157.
    • Teahan CG, Totty N, Casimir CM, Segal AW (1990). Purification of the 47 kDa phosphoprotein associated with the NADPH oxidase of human neutrophils.. Biochem J, 267(2), 485 - 489.
    • Bu-Ghanim HN, Casimir CM, Povey S, Segal AW (1990). The alpha subunit of cytochrome b-245 mapped to chromosome 16.. Genomics, 8(3), 568 - 570.

    1989

    • Segal AW (1989). The electron transport chain of the microbicidal oxidase of phagocytic cells and its involvement in the molecular pathology of chronic granulomatous disease.. J Clin Invest, 83(6), 1785 - 1793. doi:10.1172/JCI114083
    • Segal AW (1989). The electron transport chain of the microbicidal oxidase of phagocytic cells and its involvement in the molecular pathology of chronic granulomatous disease.. Biochem Soc Trans, 17(3), 427 - 434.
    • Heyworth PG, Shrimpton CF, Segal AW (1989). Localization of the 47 kDa phosphoprotein involved in the respiratory-burst NADPH oxidase of phagocytic cells.. Biochem J, 260(1), 243 - 248.
    • Nugent JH, Gratzer W, Segal AW (1989). Identification of the haem-binding subunit of cytochrome b-245.. Biochem J, 264(3), 921 - 924.

    1988

    • Garcia RC, Segal AW (1988). Phosphorylation of the subunits of cytochrome b-245 upon triggering of the respiratory burst of human neutrophils and macrophages.. Biochem J, 252(3), 901 - 904.
    • Segal AW (1988). Cytochrome b-245 and its involvement in the molecular pathology of chronic granulomatous disease.. Hematol Oncol Clin North Am, 2(2), 213 - 223.
    • Segal AW (1988). The microbicidal oxidase of phagocytic cells and its involvement in the molecular pathology of chronic granulomatous disease.. Prog Clin Biol Res, 282, 225 - 234.
    • Segal AW (1988). The molecular and cellular pathology of chronic granulomatous disease.. Eur J Clin Invest, 18(5), 433 - 443.

    1987

    • ROBERTS PJ, SEGAL AW (1987). THE DIGESTION OF BACTERIAL MACROMOLECULES BY PHAGOCYTIC-CELLS - THE EFFECT OF MEPACRINE AND ETHANOL. IMMUNOLOGY, 62(4), 581 - 586.
    • Roberts PJ, Isenberg DA, Segal AW (1987). Defective degradation of bacterial DNA by phagocytes from patients with systemic and discoid lupus erythematosus.. Clin Exp Immunol, 69(1), 68 - 78.

    1986

    • Segal AW, Isenberg DA, Hajirousou V, Tolfree S, Clark J, Snaith ML (1986). Preliminary evidence for gut involvement in the pathogenesis of rheumatoid arthritis?. Br J Rheumatol, 25(2), 162 - 166.
    • GARCIA RC, CROSS AR, SEGAL AW (1986). THE DEVELOPMENT OF CYTOCHROME-B-245 IN MATURING HUMAN MACROPHAGES. BIOCHEM J, 239(3), 647 - 651.
    • HEYWORTH PG, SEGAL AW (1986). FURTHER EVIDENCE FOR THE INVOLVEMENT OF A PHOSPHOPROTEIN IN THE RESPIRATORY BURST OXIDASE OF HUMAN-NEUTROPHILS. BIOCHEM J, 239(3), 723 - 731.

    1985

    • Segal AW, Heyworth PG, Cockcroft S, Barrowman MM (1985). Stimulated neutrophils from patients with autosomal recessive chronic granulomatous disease fail to phosphorylate a Mr-44,000 protein.. Nature, 316(6028), 547 - 549.
    • GARCIA RC, PETERSON CGB, SEGAL AW, VENGE P (1985). ELASTASE IN THE DIFFERENT PRIMARY GRANULES OF THE HUMAN NEUTROPHIL. BIOCHEM BIOPH RES CO, 132(3), 1130 - 1136.
    • HARPER AM, CHAPLIN MF, SEGAL AW (1985). CYTOCHROME-B-245 FROM HUMAN-NEUTROPHILS IS A GLYCOPROTEIN. BIOCHEM J, 227(3), 783 - 788.
    • SEGAL AW (1985). VARIATIONS ON THE THEME OF CHRONIC GRANULOMATOUS-DISEASE. LANCET, 1(8442), 1378 - 1383.
    • WINTERBOURN CC, GARCIA RC, SEGAL AW (1985). PRODUCTION OF THE SUPEROXIDE ADDUCT OF MYELOPEROXIDASE (COMPOUND III) BY STIMULATED HUMAN-NEUTROPHILS AND ITS REACTIVITY WITH HYDROGEN-PEROXIDE AND CHLORIDE. BIOCHEM J, 228(3), 583 - 592.

    1984

    • SEGAL AW (1984). HOW DO PHAGOCYTIC-CELLS KILL BACTERIA. MED BIOL, 62(2), 81 - 84.
    • HARPER AM, DUNNE MJ, SEGAL AW (1984). PURIFICATION OF CYTOCHROME-B-245 FROM HUMAN-NEUTROPHILS. BIOCHEM J, 219(2), 519 - 527.
    • Boghossian SH, Isenberg DA, Wright G, Snaith ML, Segal AW (1984). Effect of high-dose methylprednisolone therapy on phagocyte function in systemic lupus erythematosus.. Ann Rheum Dis, 43(4), 541 - 550.
    • GARCIA RC, SEGAL AW (1984). CHANGES IN THE SUBCELLULAR-DISTRIBUTION OF THE CYTOCHROME-B-245 ON STIMULATION OF HUMAN-NEUTROPHILS. BIOCHEM J, 219(1), 233 - 242.
    • OMORAIN C, SEGAL AW, LEVI AJ (1984). ELEMENTAL DIET AS PRIMARY-TREATMENT OF ACUTE CROHNS-DISEASE - A CONTROLLED TRIAL. BRIT MED J, 288(6434), 1859 - 1862.

    1983

    • SEGAL AW, GARCIA RC, HARPER AM, BANGA JP (1983). IODINATION BY STIMULATED HUMAN-NEUTROPHILS - STUDIES ON ITS STOICHIOMETRY, SUBCELLULAR-LOCALIZATION AND RELEVANCE TO MICROBIAL KILLING. BIOCHEM J, 210(1), 215 - 225.
    • CROSS AR, JONES OTG, GARCIA R, SEGAL AW (1983). THE SUBCELLULAR-LOCALIZATION OF UBIQUINONE IN HUMAN-NEUTROPHILS. BIOCHEM J, 216(3), 765 - 768.

    1982

    • CROSS AR, JONES OTG, GARCIA R, SEGAL AW (1982). THE ASSOCIATION OF FAD WITH THE CYTOCHROME-B-245 OF HUMAN-NEUTROPHILS. BIOCHEM J, 208(3), 759 - 763.

    1981

    • SEGAL AW (1981). AN ELECTRON-TRANSPORT SYSTEM IN THE PLASMA-MEMBRANE OF PHAGOCYTIC-CELLS - A MECHANISM FOR REGULATING VACUOLAR PH. J CLIN CHEM CLIN BIO, 19(8), 551 - 551.
    • HARPER AM, SEGAL AW, GARCIA RC (1981). CATALASE POSITIVE STAPHYLOCOCCUS-AUREUS IS KILLED BY NEUTROPHILS OF PATIENTS WITH CHRONIC GRANULOMATOUS-DISEASE. EUR J CLIN INVEST, 11(2), 14 - 14.
    • SEGAL AW, HARPER A, GARCIA R, JONES OTG, CROSS AR (1981). THE NATURE AND FUNCTION OF THE MICROBICIDAL OXIDASE SYSTEM OF NEUTROPHILS. CLIN RES PROC, 17, 187 - 189.
    • SEGAL AW, GEISOW M, GARCIA R, HARPER A, MILLER R (1981). THE RESPIRATORY BURST OF PHAGOCYTIC-CELLS IS ASSOCIATED WITH A RISE IN VACUOLAR PH. NATURE, 290(5805), 406 - 409.
    • CROSS AR, JONES OTG, HARPER AM, SEGAL AW (1981). OXIDATION-REDUCTION PROPERTIES OF THE CYTOCHROME-B FOUND IN THE PLASMA-MEMBRANE FRACTION OF HUMAN-NEUTROPHILS - A POSSIBLE OXIDASE IN THE RESPIRATORY BURST. BIOCHEM J, 194(2), 599 - 606.