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Dr Marcus Fruttiger
Personal Profile
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Profile
Research Description
Abnormal blood vessel growth in the retina is a pathological feature in numerous blinding diseases. It is therefore important to understand the cellular and molecular mechanisms that control vascular growth in the retina. We combine human tissue analysis and murine model systems based on reverse genetics and transgenic approaches to study the role of specific genes in development and disease of the retinal vasculature. We are particularly interested in interactions between blood vessels and the non-vascular components of the retina such as glial cells, neurons and the retinal pigment epithelium which are known to play important roles in controlling vessel behaviour in the retina. Knowledge gained from such basic research will be an important foundation for the development of future therapeutic approaches aiming to cure vascular pathologies in the human retina.
Research Activities
Angiogenesis
Hugh Davson Professor of Biomedical Research
Signal Transduction in Vascular Endothelial Cells
eye development
eye pathology
retinal glia
retinopathies
vascular cells
Education Description
UCL Collaborators
Prof John Greenwood; Dr Virginia Calder; Dr Leonid Nikitenko; Dr Patric Turowski; Prof Arne Akbar; Prof Karl Matter; Prof Maria Balda; Prof Stephen Moss
External Collaborators
Publications
- Fantin A, Vieira JM, Plein A, Denti L, Fruttiger M, Pollard JW, Ruhrberg C (2013). NRP1 acts cell autonomously in endothelium to promote tip cell function during sprouting angiogenesis.. Blood, 121(12), 2352 - 2362. doi:10.1182/blood-2012-05-424713
- Sim DA, Keane PA, Zarranz-Ventura J, Fung S, Powner MB, Platteau E, Bunce CV, Fruttiger M, Patel PJ, Tufail A, Egan CA (2013). The effects of macular ischemia on visual acuity in diabetic retinopathy.. Invest Ophthalmol Vis Sci, 54(3), 2353 - 2360. doi:10.1167/iovs.12-11103
- Sim DA, Keane PA, Mehta H, Fung S, Zarranz-Ventura J, Fruttiger M, Patel PJ, Egan CA, Tufail A (2013). Repeatability and reproducibility of choroidal vessel layer measurements in diabetic retinopathy using enhanced depth optical coherence tomography.. Invest Ophthalmol Vis Sci, 54(4), 2893 - 2901. doi:10.1167/iovs.12-11085
- McKenzie JA, Fruttiger M, Abraham S, Lange CA, Stone J, Gandhi P, Wang X, Bainbridge J, Moss SE, Greenwood J (2012). Apelin is required for non-neovascular remodeling in the retina.. Am J Pathol, 180(1), 399 - 409. doi:10.1016/j.ajpath.2011.09.035
- Allinson KR, Lee HS, Fruttiger M, McCarty JH, Arthur HM (2012). Endothelial expression of TGFβ type II receptor is required to maintain vascular integrity during postnatal development of the central nervous system.. PLoS One, 7(6), e39336 - . doi:10.1371/journal.pone.0039336
- Len AC, Powner MB, Zhu L, Hageman GS, Song X, Fruttiger M, Gillies MC (2012). Pilot application of iTRAQ to the retinal disease Macular Telangiectasia.. J Proteome Res, 11(2), 537 - 553. doi:10.1021/pr200889t
- Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW (2012). Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.. Development, 139(13), 2340 - 2350. doi:10.1242/dev.070813
- Powner MB, Vevis K, McKenzie JAG, Gandhi P, Jadeja S, Fruttiger M (2012). Visualization of gene expression in whole mouse retina by in situ hybridization. NATURE PROTOCOLS, 7(6), 1086 - 1096. doi:10.1038/nprot.2012.050
- Reis M, Czupalla CJ, Ziegler N, Devraj K, Zinke J, Seidel S, Heck R, Thom S, Macas J, Bockamp E, Fruttiger M, Taketo MM, Dimmeler S, Plate KH, Liebner S (2012). Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression.. J Exp Med, 209(9), 1611 - 1627. doi:10.1084/jem.20111580
- Shen W, Fruttiger M, Zhu L, Chung SH, Barnett NL, Kirk JK, Lee S, Coorey NJ, Killingsworth M, Sherman LS, Gillies MC (2012). Conditional Müllercell ablation causes independent neuronal and vascular pathologies in a novel transgenic model.. J Neurosci, 32(45), 15715 - 15727. doi:10.1523/JNEUROSCI.2841-12.2012
- Fruttiger MA, Jadeja S (2012). Notch signaling in vascular development. In Abraham D, Handler C, Dashwood M, Coghlan G (Ed.), Translational vascular medicine (pp. 45 - 57). : Springer London. doi:10.1007/978-0-85729-920-8_4
- Powner MB, Scott A, Zhu M, Munro PM, Foss AJ, Hageman GS, Gillies MC, Fruttiger M (2011). Basement membrane changes in capillaries of the ageing human retina.. Br J Ophthalmol, 95(9), 1316 - 1322. doi:10.1136/bjo.2011.204222
- Tammela T, Zarkada G, Nurmi H, Jakobsson L, Heinolainen K, Tvorogov D, Zheng W, Franco CA, Murtomäki A, Aranda E, Miura N, Ylä-Herttuala S, Fruttiger M, Mäkinen T, Eichmann A, Pollard JW, Gerhardt H, Alitalo K (2011). VEGFR-3 controls tip to stalk conversion at vessel fusion sites by reinforcing Notch signalling.. Nat Cell Biol, 13(10), 1202 - 1213. doi:10.1038/ncb2331
- D'Amico G, Robinson SD, Germain M, Reynolds LE, Thomas GJ, Elia G, Saunders G, Fruttiger M, Tybulewicz V, Mavria G, Hodivala-Dilke KM (2010). Endothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.. PLoS One, 5(3), e9766 - . doi:10.1371/journal.pone.0009766
- Kuhnert F, Mancuso MR, Shamloo A, Wang HT, Choksi V, Florek M, Su H, Fruttiger M, Young WL, Heilshorn SC, Kuo CJ (2010). Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124.. Science, 330(6006), 985 - 989. doi:10.1126/science.1196554
- Powner MB, Gillies MC, Tretiach M, Scott A, Guymer RH, Hageman GS, Fruttiger M (2010). Perifoveal müller cell depletion in a case of macular telangiectasia type 2.. Ophthalmology, 117(12), 2407 - 2416. doi:10.1016/j.ophtha.2010.04.001
- Parmalee NL, Schubert C, Merriam JE, Allikmets K, Bird AC, Gillies MC, Peto T, Figueroa M, Friedlander M, Fruttiger M, Greenwood J, Moss SE, Smith LEH, Toomes C, Inglehearn CF, Allikmets R (2010). Analysis of candidate genes for macular telangiectasia type 2. MOL VIS, 16(289-90), 2718 - 2726.
- Tavora B, Batista S, Reynolds LE, Jadeja S, Robinson S, Kostourou V, Hart I, Fruttiger M, Parsons M, Hodivala-Dilke KM (2010). Endothelial FAK is required for tumour angiogenesis. EMBO MOL MED, 2(12), 516 - 528. doi:10.1002/emmm.201000106
- Scott A, Powner MB, Gandhi P, Clarkin C, Gutmann DH, Johnson RS, Ferrara N, Fruttiger M (2010). Astrocyte-derived vascular endothelial growth factor stabilizes vessels in the developing retinal vasculature.. PLoS One, 5(7), e11863 - . doi:10.1371/journal.pone.0011863
- Scott A, Fruttiger M (2010). Oxygen-induced retinopathy: a model for vascular pathology in the retina.. Eye (Lond), 24(3), 416 - 421. doi:10.1038/eye.2009.306
- Mahmoud M, Allinson KR, Zhai Z, Oakenfull R, Ghandi P, Adams RH, Fruttiger M, Arthur HM (2010). Pathogenesis of arteriovenous malformations in the absence of endoglin.. Circ Res, 106(8), 1425 - 1433. doi:10.1161/CIRCRESAHA.109.211037
- Benedito R, Roca C, Sorensen I, Adams S, Gossler A, Fruttiger M, Adams RH (2009). The notch ligands Dll4 and Jagged1 have opposing effects on angiogenesis. Cell, 137(6), 1124 - 1135.
- Longbottom R, Fruttiger M, Douglas RH, Martinez-Barbera JP, Greenwood J, Moss SE (2009). Genetic ablation of retinal pigment epithelial cells reveals the adaptive response of the epithelium and impact on photoreceptors.. Proc Natl Acad Sci U S A, 106(44), 18728 - 18733. doi:10.1073/pnas.0902593106
- Arthur H, Allinson K, Mahmoud M, Zhai Z, Oakenfull R, Fruttiger M (2009). Endoglin deficiency leads to arteriovenous malformations during angiogenesis. doi:10.1016/j.mod.2009.06.508
- Liebner S, Corada M, Bangsow T, Babbage J, Taddei A, Czupalla CJ, Reis M, Felici A, Wolburg H, Fruttiger M, von Melchner H, Plate KH, Gerhardt H, Dejana E (2008). W(n)over-tildet/beta-Catenin signalling controls development and maintenance of the blood-brain barrier.
- Liebner S, Corada M, Bangsow T, Babbage J, Taddei A, Czupalla CJ, Reis M, Felici A, Wolburg H, Fruttiger M, Taketo MM, von Melchner H, Plate KH, Gerhardt H, Dejana E (2008). Wnt/beta-catenin signaling controls development of the blood-brain barrier. J.Cell Biol., 183(3), 409 - 417.
- Fruttiger MA (2008). VEGF gene regulation. In Ruhrberg C (Ed.), VEGF in development (pp. 30 - 39). : Springer New York. doi:10.1007/978-0-387-78632-2_3
- Claxton S, Kostourou V, Jadeja S, Chambon P, Hodivala-Dilke K, Fruttiger M (2008). Efficient, inducible Cre-recombinase activation in vascular endothelium. Genesis., 46(2), 74 - 80.
- Fruttiger M (2007). Development of the retinal vasculature. Angiogenesis, 10(2), 77 - 88. doi:10.1007/s10456-007-9065-1
- Vana AC, Flint NC, Harwood NE, Le TQ, Fruttiger M, Armstrong RC (2007). Platelet-derived growth factor promotes repair of chronically demyelinated white matter. J.Neuropathol.Exp.Neurol., 66(11), 975 - 988.
- Fruttiger M (2007). Mechanisms of ocular angiogenesis.
- Fruttiger M (2006). Development of the retinal vasculature.
- Claxton S, Fruttiger MA (2005). Oxygen modifies artery differentiation and network morphogenesis in the retinal vasculature. Developmental dynamics, 233(3), 822 - 828. doi:10.1002/dvdy.20407
- Hurskainen M, Eklund L, Hagg PO, Fruttiger M, Sormunen R, Ilves M, Pihlajaniemi T (2005). Abnormal maturation of the retinal vasculature in type XVIII collagen/endostatin deficient mice and changes in retinal glial cells due to lack of collagen types XV and XVIII. The FASEB journal, 19(11), 1564 - 1566. doi:10.1096/fj.04-3101fje
- West H, Richardson WD, Fruttiger MA (2005). Stabilization of the retinal vascular network by reciprocal feedback between blood vessels and astrocytes.. Development, 132, 1855 - 1862. doi:10.1242/dev.01732
- Fruttiger M (2005). Role of VEGF in diabetic retinopathy.
- Fruttiger M (2005). Developmental versus Pathologic Retinal Neovascularization.
- Claxton S, Fruttiger MA (2004). Periodic Delta-like 4 expression in developing retinal arteries. Gene expression patterns, 5(1), 123 - 127. doi:10.1016/j.modgep.2004.05.004
- Woodruff RF, Fruttiger M, Richardson WD, Franklin RJM (2004). Platelet-derived growth factor regulates oligodendrocyte progenitor numbers in adult CNS and their response following CNS demyelination. Molecular and Cellular Neuroscience, 25, 252 - 262.
- Claxton S, Fruttiger M (2003). Role of arteries in oxygen induced vaso-obliteration. Experimental eye research, 77, 305 - 311. doi:10.1016/S0014-4835(03)00153-2
- Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A, Jeltsch M, Mitchell C, Alitalo K, Shima D, Betsholtz C (2003). VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. The Journal of Cell Biology, , - . doi:10.1083/jcb.200302047
- Stalmans I, Ng YS, Rohan R, Fruttiger M, Bouche A, Yuce A, Fujisawa H, Hermans B, Shani M, Jansen S, Hicklin D, Anderson DJ, Gardiner T, Hammes HP, Moons L, Dewerchin M, Collen D, Carmeliet P, D 'Amore PA (2002). Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms. Journal of Clinical Investigation, 109(3), 327 - 336. doi:10.1172/JCI0214362
- Fruttiger MA (2002). Development of the mouse retinal vasculature: angiogenesis versus vasculogenesis. Investigative Ophthalmology and Visual Science, 43, 522 - 527.
- Fruttiger M, Calver AR, Richardson WD (2000). Platelet-derived growth factor is constitutively secreted from neuronal cell bodies but not from axons.. Curr Biol, 10(20), 1283 - 1286.
- Biscette O, Vongsvivut P, Fruttiger M, Fox M, Richardson W, Hitchcock P (2000). A murine model of retinal coloboma..
- Fruttiger M, Calver AR, Richardson WD (2000). Platelet-derived growth factor is constitutively secreted from neuronal cell bodies but not from axons. Current Biology, 10(20), 1283 - 1286.
- Fruttiger M, Karlsson L, Hall AC, Abramsson A, Calver AR, Bostrom H, Willetts K, Bertold C, Heath JK, Betsholtz C, Richardson WD (1999). Defective oligodendrocyte development and severe hypomyelination in PDGF-A knockout mice. Development, 126, 457 - 467.
- Richardson W, Calver A, Fruttiger M, Hall A, Pringle N, Sun T, van Heyningen P (1998). Specification and growth of oligodendrocyte lineage cells.
- Calver AR, Hall AC, Fruttiger M, Yu WP, Richardson WD (1997). Control of cell number in the oligodendrocyte lineage.
- Fruttiger M, Calver AR, Krüger WH, Mudhar HS, Michalovich D, Takakura N, Nishikawa S, Richardson WD (1996). PDGF mediates a neuron-astrocyte interaction in the developing retina.. Neuron, 17(6), 1117 - 1131.
- Friedlander M, Theesfeld CL, Sugita M, Fruttiger M, Thomas MA, Chang S, Cheresh DA (1996). Involvement of integrins alpha v beta 3 and alpha v beta 5 in ocular neovascular diseases.. Proc Natl Acad Sci U S A, 93(18), 9764 - 9769.
- Schäfer M, Fruttiger M, Montag D, Schachner M, Martini R (1996). Disruption of the gene for the myelin-associated glycoprotein improves axonal regrowth along myelin in C57BL/Wlds mice.. Neuron, 16(6), 1107 - 1113.
- Friedlander M, Theesfeld CL, Sugita M, Thomas MA, Chang S, Coli G, Fruttiger MA, Richardson WD, Brooks PC, Cheresh DA (1996). Integrins alpha(v)beta(3) and alpha(v)beta(5) are selectively expressed in active human retinal and choroidal neovascular membranes.
- Fruttiger M, Montag D, Schachner M, Martini R (1995). Crucial role for the myelin-associated glycoprotein in the maintenance of axon-myelin integrity.. Eur J Neurosci, 7(3), 511 - 515.
- Fruttiger M, Schachner M, Martini R (1995). Tenascin-C expression during wallerian degeneration in C57BL/Wlds mice: possible implications for axonal regeneration.. J Neurocytol, 24(1), 1 - 14.
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