Dr Bambos Charalambous

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Personal Profile

Name: Bambos Charalambous Email: b.charalambous@ucl.ac.uk
Title: Dr Tel: 0044 207794 0500
Department: Research Department of Infection Fax:
Position: Senior Lecturer Address: University College Medical School, Royal Free Campus, London, NW3 2PF
Research Domain: Global Health, Infection, Immunology & Inflammation, Personalised Medicine, Populations & Lifelong Health, Reproduction & Development Web Page: Personal Web Page

Profile

Research Description

My work brings together basic and clinical scientists to undertake research on host-pathogen interactions, antibiotic resistance and vaccine development. My research includes biofilms and quorum sensing in colonisation of the nasopharynx in children by capsulated bacteria such as Streptococcus pneumoniae. During colonisation evolution of antibiotic resistance and transmission occur. Vaccine development is targeted towards a non-serotype vaccine against S. pneumoniae that will provide broad protection at a lower cost than existing vaccines. This would make the vaccine globally accessible to include resource limited developing countries. Host-pathogen studies also extend to the inflammatory role of endogenous sources of Gram-negative endotoxins (LPS) during chronic disease such as liver tranplantation and chronic heart disease are being investigated.

Research Activities

Host-pathogen interaction in infection

Education Description

Teach on various under- and post-graduate taught and laboratory based courses in Biomedical Sciences. Academic Director for a new MSc in "Frontiers in Infection & Immunity" planned to be introduced in September 2009. Organiser for a number student selected modules on vaccines, antibioic resistance, and a laboratory-based module on "Challenge of antibioic resistance". I run a laboratory based Graduate Skills course on Basic bacteriology and recombinant DNA. Supervise MSc/PhD students. Supervise summer research students on scholarships. Supervise Nuffield Bursary scholars. Invited speaker to local schools to promote higher education in the Biomedical Science in particular Microbiology.

UCL Collaborators

External Collaborators

Publications

    2013

    • Shorten RJ, McGregor AC, Platt S, Jenkins C, Lipman MC, Gillespie SH, Charalambous BM, McHugh TD (2013). When is an outbreak not an outbreak? Fit, divergent strains of Mycobacterium tuberculosis display independent evolution of drug resistance in a large London outbreak.. J Antimicrob Chemother, 68(3), 543 - 549. doi:10.1093/jac/dks430

    2012

    • Shiffman ME, Charalambous BM (2012). The search for archaeal pathogens. REVIEWS IN MEDICAL MICROBIOLOGY, 23(3), 45 - 51. doi:10.1097/MRM.0b013e328353c7c9
    • Vanstone GL, Yorgancioglu A, Wilkie L, Mouskos K, Charalambous BM, Balakrishnan I (2012). A real-time multiplex PCR assay for the rapid detection of CTX-M-type extended spectrum β-lactamases directly from blood cultures.. J Med Microbiol, 61(Pt 11), 1631 - 1632. doi:10.1099/jmm.0.045419-0
    • Charalambous BM (2012). Identifying divergent streptococcal strains using pherotype: implications for pathogen emergence and epidemiology.. Future Microbiol, 7(12), 1349 - 1354. doi:10.2217/fmb.12.114
    • Leung MH, Bryson K, Freystatter K, Pichon B, Edwards G, Charalambous BM, Gillespie SH (2012). Sequetyping: serotyping Streptococcus pneumoniae by a single PCR sequencing strategy.. J Clin Microbiol, 50(7), 2419 - 2427. doi:10.1128/JCM.06384-11
    • Charalambous BM, Leung MH (2012). Pneumococcal sepsis and nasopharyngeal carriage.. Curr Opin Pulm Med, 18(3), 222 - 227. doi:10.1097/MCP.0b013e328352103b
    • Leung MH, Ling CL, Ciesielczuk H, Lockwood J, Thurston S, Charalambous BM, Gillespie SH (2012). Streptococcus pseudopneumoniae identification by pherotype: a method to assist understanding of a potentially emerging or overlooked pathogen.. J Clin Microbiol, 50(5), 1684 - 1690. doi:10.1128/JCM.00131-12

    2011

    • Leung MH, Oriyo NM, Gillespie SH, Charalambous BM (2011). The adaptive potential during nasopharyngeal colonisation of Streptococcus pneumoniae.. Infect Genet Evol, 11(8), 1989 - 1995. doi:10.1016/j.meegid.2011.09.002
    • Chua I, Lagos M, CHARALAMBOUS B, Workman S, Chee R, Grimbacher B (2011). Pathogen-specific IgG antibody levels in immunodeficient patients receiving immunoglobulin replacement do not provide additional benefit to therapeutic management over total serum IgG. Journal of Allergy and Clinical Immunology, , - .

    2010

    • Charalambous BM, McHugh TD (2010). Immunological principles of vaccination (Theory). In Jong EC, Zuckerman JD (Ed.), Travelers' Vaccines. (pp. - ). : BC Decker Inc.
    • CHARALAMBOUS B, Balakrishnan I (2010). Traveler's Vaccines. In Zuckerman JN, Zuckerman , Jong EC (Ed.), Traveler's Vaccines (pp. 25 - 37). : Pmph USA Ltd.
    • CHARALAMBOUS B, McHugh TD (2010). Traveler's Vaccines. In Zuckerman JN, Zuckerman , Jong EC (Ed.), Traveler's Vaccines (pp. 3 - 23). : Pmph USA Ltd.
    • Mwenya DM, Charalambous BM, Phillips PP, Mwansa JC, Batt SL, Nunn AJ, Walker S, Gibb DM, Gillespie SH (2010). Impact of cotrimoxazole on carriage and antibiotic resistance of Streptococcus pneumoniae and Haemophilus influenzae in HIV-infected children in Zambia.. Antimicrob Agents Chemother, 54(9), 3756 - 3762. doi:10.1128/AAC.01409-09
    • Charalambous BC, Oriyo NM (2010). Unravelling the ecology of antibiotic resistant bacteria in the nasopharynx.. In Gillespie SH, McHugh TD (Ed.), Antibiotic Resistance Protocols (pp. 13 - 26). : Humana Pr Inc. doi:10.1007/978-1-60327-279-7

    2008

    • Charalambous BM, Oriyo NM, Gillespie SH (2008). How valid is single-colony isolation for surveillance of Streptococcus pneumoniae. Journal of Clinical Microbiology, 46(7), 2467 - 2468. doi:10.1128/JCM.00838-08
    • Chua I, Lagos M, Charalambous BM, Workman S, Tarzi M, Chee R, Grimbacher B (2008). Using pathogen-specific antibody levels to monitor the adequacy of immunoglobulin replacement in antibody deficient patients.

    2007

    • Charalambous BM, Stephens RCM, Feavers IM, Montgomery HE (2007). Role of bacterial endotoxin in chronic heart failure: the gut of the matter. SHOCK, 28(1), 15 - 23. doi:10.1097/shk.0b013e318033ebc5
    • Charalambous BM (2007). Streptococcus pneumoniae: pathogen or protector?. Reviews in Medical Microbiology, 18(4), 73 - 78.

    2005

    • Tiwana H, Clow KJ, Hall C, Feavers IM, Charalambous BM (2005). The immunogenicity of a conformationally restricted peptide mimetic of meningococcal lipooligosaccharide.. Scand J Immunol, 62(4), 385 - 392. doi:10.1111/j.1365-3083.2005.01671.x
    • Batt SL, Charalambous BM, McHugh TD, Martin S, Gillespie SH (2005). Novel PCR-restriction fragement length polymorphism method to determine serotypes or serogroups of Streptococcus pneumoniae isolates. Journal of Clinical Microbiology, 43(6), 2656 - 2661. doi:10.1128/JCM.43.6.2656-2661.2005

    2004

    • Charalambous BM, McHugh TD (2004). Immunological principles of vaccination (Theory). In Jong EC, Zuckerman JD (Ed.), Travelers' Vaccines. Section 1: Vaccine Immunology (pp. 1 - 19). : BC Decker Inc.

    2003

    • Batt SL, Charalambous BM, Solomon AW, Knirsch C, Massae PA, Safari S, Sam NE, Everett D, Mabey DC, Gillespie SH (2003). Impact of azithromycin administration for trachoma control on the carriage of antibiotic-resistant Streptococcus pneumoniae. Antimicrobial Agents and Chemotherapy, 47(9), 2765 - 2769. doi:10.1128/AAC.47.9.2765-2769.2003
    • Doré CJ, Nunn AJ, Gillespie SH, Gosling RD, Charalambous BM (2003). Bactericidal activity of antituberculosis drugs. American Journal of Respiratory and Critical Care Medicine, 167(4), 663 - 663.
    • Gillespie SH, Charalambous BM, Gosling RD (2003). A reiterative method for calculating bactericidal activity. AM J RESP CRIT CARE, 167(4), 664 - 664.
    • Gillespie SH, Charalambous BM (2003). A novel method for evaluating the antimicrobial activity of tuberculosis treatment regimens. International Journal of Tuberculosis and Lung Disease, 7(7), 684 - 689.
    • Charalambous BM, Batt SL, Peek AC, Mwerinde H, Sam N, Gillespie SH (2003). Quantitative validation of media for the transportation and storage of Streptococcus pneumoniae. Journal of Clinical Microbiology, 41(12), 5551 - 5556.

    2002

    • Gillespie SH, Gosling RD, Charalambous BM (2002). A reiterative method for calculating the early bactericidal activity of antituberculosis drugs. American Journal of Respiratory and Critical Care Medicine, 166(1), 31 - 35.
    • Brett PJ, Tiwana H, Feavers IM, Charalambous BM (2002). Characterization of oligopeptides that cross-react with carbohydrate- specific antibodies by real time kinetics, in-solution competition enzyme-linked immunosorbent assay, and immunological analyses. Journal of Biological Chemistry, 277(23), 20468 - 20476. doi:10.1074/jbc.M200387200
    • Charalambous BM (2002). Meningitis B vaccine.

    2001

    • Charalambous BM, Feavers IM (2001). Mimotope vaccines. Journal of Medical Microbiology, 50(11), 937 - 939.
    • Brett P, Feavers IM, Charalambous BM (2001). Identification of peptides that mimic Neisseria meningitidis LOS epitopes via the use of combinatorial phage display libraries. In Pollard AJ, Maiden MCJ (Ed.), Methods in molecular medicine: Meningococcal vaccines methods and protocols (pp. 181 - 197). : Humana Press.
    • Suker J, Charalambous BM (2001). Application of optical biosensor techniques to the characterisation of porA antibody binding kinetics. In Pollard AJ, Maiden MCJ (Ed.), Methods in molecular medicine: Meningococcal vaccines methods and protocols (pp. 129 - 143). : Humana Press.

    2000

    • Charalambous BM, Feavers IM (2000). Peptide mimics elicit antibody responses against the outer membrane lipooligosaccharide of group B Neisseria meningitidis. FEMS Microbiology Letters, 191(1), 45 - 50.

    1999

    • Charalambous BM, Evans J, Feavers IM, Maiden MCJ (1999). Comparative analysis of two meningococcal immunotyping monoclonal antibodies by resonant mirror biosensor and antibody gene sequencing.. Clinical and Diagnostic Laboratory Immunology, 6(6), 838 - 843.

    1996

    • Yallop CA, Charalambous BM (1996). Nutrient utilization and transport in the thermoacidophilic archaeon Sulfolobus shibatae. MICROBIOL-UK, 142, 3373 - 3380.

    1995

    • Huddleston S, Yallop CA, Charalambous BM (1995). The identification and partial characterisation of a novel inducible extracellular thermostable esterase from the archaeon Sulfolobus shibatae.. Biochemical and Biophysical Research Communications, 216(2), 495 - 500.
    • Shatwell KP, Charalambous BM, McDonald TP, Henderson PJF (1995). Cloning, sequencing, and expression of the araE gene of Klebsiella oxytoca 8017, which encodes arabinose-H+ symport activity.. Journal of Bacteriology, 177, 5379 - 5380.
    • Huddleston S, Robertson S, Dobson C, Kwong YP, Charalambous BM (1995). Structural and functional stability of horseradish peroxidase.. Biochem Soc Trans, 23(1), 108S - .
    • HUDDLESTON S, ROBERTSON S, DOBSON C, KWONG FYP, CHARALAMBOUS BM (1995). STRUCTURAL AND FUNCTIONAL STABILITY OF HORSERADISH-PEROXIDASE.

    1994

    • PRESTON RAJ, SAMI AJ, CHARALAMBOUS BM, BALDWIN SA (1994). PRODUCTION OF FUNCTIONAL GLUT1 BY COEXPRESSION OF N-TERMINAL AND C-TERMINAL HALF MOLECULES IN XENOPUS OOCYTES.
    • Preston RA, Sami AJ, Charalambous BM, Baldwin SA (1994). Production of functional GLUT1 by co-expression of N- and C-terminal half molecules in Xenopus oocytes.. Biochem Soc Trans, 22(3), 276S - .

    1993

    • MITROPHANOUS K, CHARALAMBOUS BM (1993). MOLECULAR STUDIES OF THE FORMYLATED PEPTIDE RECEPTOR. PROTEIN ENG, 6, 82 - 82.
    • Mitrophanous K, Charalambous BM (1993). Molecular studies on chemotactic receptors.. Biochem Soc Trans, 21(2), 297 - 299.

    1992

    • Henderson PJ, Baldwin SA, Cairns MT, Charalambous BM, Dent HC, Gunn F, Liang WJ, Lucas VA, Martin GE, McDonald TP (1992). Sugar-cation symport systems in bacteria.. International Review of Cytology, 137, 149 - 208.
    • Yi C, Charalambous BM, Emery VC, Baldwin SA (1992). Characterization of functional human erythrocyte-type glucose transporter (GLUT1) expressed in insect cells using a recombinant baculovirus.. Biochemical Journal, 283, 643 - 646.

    1989

    • Charalambous BM, Maiden MC, McDonald TP, Cunningham IJ, Henderson PJ (1989). Detection of proton-linked sugar transport proteins in Enterobacteriaceae.. Biochem Soc Trans, 17(3), 441 - 444.

    1988

    • MCPHERSON MJ, CHARALAMBOUS BM (1988). FUNCTIONAL-ANALYSIS OF THE STARCH DEBRANCHING ENZYME PULLULANASE. BIOCHEM SOC T, 16(5), 723 - 724.
    • CHARALAMBOUS BM, MCPHERSON MJ (1988). MULTIMOLECULAR ORGANIZATION OF THE BACTERIAL ENZYME PULLULANASE. BIOCHEM SOC T, 16(5), 722 - 723.
    • Charalambous BM, Keen JN, McPherson MJ (1988). Collagen-like sequences stabilize homotrimers of a bacterial hydrolase.. EMBO J, 7(9), 2903 - 2909.

    1987

    • Charalambous BM, Wheeler KP (1987). Sodium ions protect a membrane transport protein from proteolysis.. Biochim Biophys Acta, 903(3), 441 - 448.

    1986

    • CHARALAMBOUS BM, WHEELER KP (1986). SELECTIVE DIGESTION OF A MEMBRANE-TRANSPORT PROTEIN. BIOCHEM SOC T, 14(4), 754 - 754.

    1985

    • WHEELER KP, CHARALAMBOUS BM, NICKLIN SJ (1985). APPARENT RECONSTITUTION OF A MEMBRANE-TRANSPORT PROTEIN. BIOCHEM SOC T, 13(1), 264 - 265.
    • Charalambous BM, Wheeler KP (1985). Sodium-induced conformation changes in membrane transport proteins.. FEBS Lett, 189(2), 163 - 166.

    1984

    • Morgan K, Foord SM, Spurlock G, Charalambous BM, Dieguez C, Scanlon MF, Mir MA (1984). Release of an active sodium transport inhibitor (ASTI) from rat hypothalamic cells in culture.. Endocrinology, 115(4), 1642 - 1644. doi:10.1210/endo-115-4-1642
    • MORGAN K, SPURLOCK G, CHARALAMBOUS BM, MIR MA (1984). DOES OXYTOCIN COUNTERACT THE INHIBITORY ACTIVITY OF THE HYPOTHALAMIC SODIUM-TRANSPORT INHIBITOR. CLIN SCI, 66(2), P17 - P18.
    • Mir MA, Morgan K, Charalambous BM, Spurlock G, Newcombe RG (1984). Sodium-potassium-ATPase and obesity.. N Engl J Med, 310(8), 528 - 529. doi:10.1056/NEJM198402233100816
    • Charalambous BM, Morgan K, Spurlock G, Mir MA (1984). Erythrocyte sodium pump activity in human obesity.. Clin Chim Acta, 141(2-3), 179 - 187.
    • Charalambous BM, Webster DJ, Mir MA (1984). Elevated skeletal muscle sodium-potassium-ATPase in human obesity.. Clin Chim Acta, 141(2-3), 189 - 195.

    1983

    • Tirlapur VG, Gicheru K, Charalambous BM, Evans PJ, Mir MA (1983). Packed cell volume, haemoglobin, and oxygen saturation changes in healthy smokers and non-smokers.. Thorax, 38(10), 785 - 787.
    • MORGAN K, FOORD SM, SPURLOCK G, CHARALAMBOUS BM, DIEGUEZ C, SCANLON MF, MIR MA (1983). FETAL HYPOTHALAMIC CELLS IN CULTURE SECRETE - A SODIUM-TRANSPORT INHIBITOR. CLIN SCI, 65(3), P67 - P67.
    • CHARALAMBOUS BM, MORGAN K, SPURLOCK G, WAN S, WEBSTER D, MIR MA (1983). NA+/K+ ATPASE IN HUMAN-LIVER AND SKELETAL-MUSCLE. CLIN SCI, 65(3), P67 - P67.

    1982

    • MIR MA, CHARALAMBOUS BM, MORGAN K (1982). ERYTHROCYTE SODIUM-POTASSIUM-ATPASE AND OBESITY - REPLY. NEW ENGL J MED, 306(13), 809 - 810.
    • Charalambous BM, Mir MA (1982). An improved procedure for the preparation and measurement of (Na+ + K+)-ATPase in human erythrocytes.. Biochim Biophys Acta, 691(1), 71 - 82.

    1981

    • MIR MA, EVANS PJ, CHARALAMBOUS BM, MORGAN K (1981). STUDY OF ERYTHROCYTE SODIUM-POTASSIUM ATPASE AND SODIUM-TRANSPORT IN DIABETES-MELLITUS. DIABETOLOGIA, 21(5), 512 - 512.
    • MIR MA, CHARALAMBOUS BM, NICHOLAS N (1981). AN EXPERIMENTAL APPROACH TO LEUKEMIC CARDIOMYOPATHY - EFFECTS OF LEUKEMIC BLAST CELL EXTRACT ON ULTRASTRUCTURAL-CHANGES AND MITOCHONDRIAL RESPIRATION OF RAT-HEART. CLIN SCI, 60(3), P28 - P28.
    • Mir MA, Charalambous BM, Morgan K, Evans PJ (1981). Erythrocyte sodium-potassium-ATPase and sodium transport in obesity.. N Engl J Med, 305(21), 1264 - 1268. doi:10.1056/NEJM198111193052107
    • MIR MA, EVANS PJ, MAHLER RF, CHARALAMBOUS BM, MORGAN K (1981). A COMPARATIVE-STUDY OF ERYTHROCYTE SODIUM-POTASSIUM ATPASE AND SODIUM-TRANSPORT IN OBESE AND THIN NORMAL SUBJECTS. CLIN SCI, 61(3), P18 - P18.