Dr Clare Jolly

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Personal Profile

Name: Clare Jolly Email: c.jolly@ucl.ac.uk
Title: Dr Tel: 0044 203 108 2145
Department: Div of Infection & Immunity Fax:
Position: Senior Research Fellow Address: Cruciform Building, Gower Street, London, WC1E 6BT
Research Domain: Infection Immunology & Inflammation Web Page: Personal Web Page

Profile

Research Description

UNAIDS estimates around 33 million people are living with HIV/AIDS and approximately 25 million have died. While the ultimate goal remains the development of an effective HIV-1 vaccine, there is also a need for new and improved anti-viral strategies and this requires a better understanding of the molecular cell biology and pathogenesis of HIV-1 infection.



The research interests of my laboratory are broadly aimed at identifying cellular factors (and pathways) that regulate HIV-1 assembly in, and dissemination between, CD4 T cells - the main targets for HIV-1 infection in vivo. We are particularly interested in molecules that are involved in trafficking the HIV-1 envelope glycoprotein (Env), and the capsid protein (Gag) to sites of viral assembly in order to temporally and spatially orchestrate new virus production. Previous work by us and others has identified direct cell-cell spread of HIV-1 between T cells as an efficient mode of viral dissemination that takes place across a multimolecular structure called the virological synapse (VS)(see publications). The VS is characterised by cytoskeleton-driven recruitment of viral proteins, adhesion molecules and entry receptors (CD4 and chemokine receptors) to the site of contact between an HIV-1-infected T cell and a susceptible target T cell. This promotes rapid infection of the target cell by focussing virus assembly and budding to the site of cell-cell contact and may allow HIV-1 to evade aspects of the humoral immune response. The VS shares many similarities with a well-described structure called the immunological synapse (IS) and we hypothesise that HIV-1 spread at the VS may involve related cellular machinery, and that HIV-1 hijacks elements of the regulated secretory pathway to transport viral proteins and coordinate assembly and egress. Our current research is focussed on delineating the contribution of secretory pathways and associated molecules to HIV-1 pathogenesis in T cells. We are also seeking to identify new cellular proteins that may be important in HIV-1 egress and T cell secretion using high-throughput RNAi screening.

Research Activities

The secretory pathway and HIV-1 egress

Education Description

I lecture on the Molecular Virology course and MSc programs. 

UCL Collaborators

External Collaborators

Publications

    2011

    • Zhou L, Sokolskaja E, Jolly C, James W, Cowley SA, Fassati A (2011). Transportin 3 promotes a nuclear maturation step required for efficient HIV-1 integration.. PLoS Pathog, 7(8), e1002194 - . doi:10.1371/journal.ppat.1002194
    • Jolly CL, Welsch S, Michor S, Sattentau QJ (2011). The Regulated Secretory Pathway in CD4+ T cells Contributes to Human Immunodeficiency Virus Type-1 Cell-to-Cell Spread at the Virological Synapse. PLoS Pathogens, 7, e10022226 - . doi:10.1371/journal.ppat.1002226
    • JOLLY C (2011). Cell-to-cell transmission of retroviruses: Innate immunity and interferon-induced restriction factors. Virology, 411(2), 251 - 259. doi:10.1016/j.virol.2010.12.031

    2010

    • Jolly C, Booth NJ, Neil SJ (2010). Cell-Cell spread of Human Immunudeficiency Virus Type 1 overcomes tetherin/BST-2-mediated restriction in T cells. Journal of Virology, 84(23), 12185 - 12199. doi:10.1128/JVI.01447-10
    • Gonzales N, Bermejo M, Calonge E, Jolly C, Arenzena-Seisdedos F, Pablos JL, Sattentau QJ, Alcami J (2010). SDF-1/CXCL12 production by mature dendritic cells inhibits the propagation of X4-tropic HIV-1 isolates at the dendritic cell-T-cell infectious synapse. Journal of Virology, 84(9), 4341 - 4351.
    • Jolly C (2010). T Cell Polarization at the Virological Synapse. Viruses, 2(6), 1261 - 1278. doi:10.3390/v2061261
    • Martin N, Welsch S, Jolly C, Briggs JA, Vaux D, Sattentau QJ (2010). Virological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition. Journal of Virology, 84(7), 3516 - 3527. doi:10.1128/JVI.02651-09.
    • Sowinski S, Alakoskela JM, Jolly C, Davis DM (2010). Optimized methods for imaging membrane nanotubes between T cells and trafficking of HIV-1.. Methods, , - .
    • Jolly C, Sattentau QJ (2010). Attachment factors. In Pohlmann S, Simmons G (Ed.), Virus Entry into Host Cells (pp. - ). : Landes Bioscience.

    2008

    • Sowinski S, Jolly C, Berninghausen O, Purboo MA, Chauveau A, Kohler K, Oddos S, Eissmann P, Brodsky FM, Hopkins C, Onfelt B, Sattentau Q, Davis DM (2008). Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. Nature Cell Biology, 10(2), 211 - 219. doi:10.1038/ncb1682

    2007

    • Jolly C, Sattentau QJ (2007). Human immunodeficiency virus type 1 assembly, budding, and cell-cell spread in T cells take place in tetraspanin-enriched plasma membrane domains. Journal of Virology, 81(15), 7873-7884 - . doi:10.1128/JVI.01845-06
    • Jolly C, Sattentau QJ (2007). Regulated secretion from CD4+ T cells. Trends in Immunology, 28(11), 474 - 481. doi:10.1016/j.it.2007.08.008
    • Jolly C, Mitar I, Sattentau QJ (2007). Adhesion molecule interactions facilitate human immunodeficiency virus type 1-induced virological synapse formation between T cells. Journal of Virology, 81(24), 13916-21 - . doi:10.1128/JVI.01585-07
    • Jolly C, Mitar I, Sattentau QJ (2007). Requirement for an intact T-cell actin and tubulin cytoskeleton for efficient assembly and spread of human immunodeficiency virus type 1. Journal of Virology, 81(11), 5547-5560 - . doi:10.1128/JVI.01469-06.

    2005

    • Jolly C, Sattentau QJ (2005). Human immunodeficiency virus type 1 virological synapse formation in T cells requires lipid raft integrity. Journal of Virology, 79(18), 12088 - 12094. doi:10.1128/JVI.79.18.12088-12094.2005.

    2004

    • Jolly C, Kashefi K, Hollinshead M, Sattentau QJ (2004). HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse. Journal of Experimental Medicine, 199(2), 283 - 293. doi:10.1084/jem.20030648.
    • Jolly C, Sattentau QJ (2004). Retroviral spread by induction of virological synapses. Traffic, 5(9), 643 - 650. doi:10.1111/j.1600-0854.2004.00209.x

    2001

    • Jolly C, Beisner BM, Ozser E, Holmes IH (2001). Non-lytic extraction and characterisation of receptors for multiple strains of rotavirus. Archives of Virology, 146(7), 1307 - 1323. doi:10.1007/s007050170093
    • Jolly C, Huang JA, Holmes IH (2001). Selection of rotavirus VP4 cell receptor binding domains for MA104 cells using a phage display library. Journal of Virological Methods, 98(1), 41 - 51. doi:10.1016/S0166-0934(01)00357-3
    • Fazli A, Bradley SJ, Kiefel MJ, Jolly C, Holmes IH, von Itzstein M (2001). Synthesis and biological evaluation of sialylmimetics as rotavirus inhibitors. Journal of Medicinal Chemistry, 44(20), 3292 - 3301. doi:10.1021/jm0100887

    2000

    • Jolly C, Beisner BM, Holmes IH (2000). Rotavirus infection of MA104 cells is inhibited by Ricinus lectin and separately expressed single binding domains. Virology, 275(1), 89 - 97. doi:10.1006/viro.2000.0470