Prof Christopher Danpure

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Personal Profile

Name: Christopher Danpure Email: c.danpure@ucl.ac.uk
Title: Prof Tel: 020-7679-7936
Department: Div of Biosciences Fax:
Position: Emeritus Professor Molecular Cell Biology Address: Division of Biosciences, UCL, Gower St, London, WC1E 6BT
Research Domain: Basic Life Sciences Web Page: Personal Web Page

Profile

Research Description

For the past 20 years I have worked on various aspects of the variable peroxisomal and mitochondrial targeting of the intermediary metabolic enzyme alanine:glyoxylate aminotransferase (AGT) and its relationship to hereditary calcium oxalate kidney stone diseases. More specifically, my research has concentrated on two areas:- 1) the molecular basis for the episodic fluctuations in AGT targeting during mammalian evolution under the influence of dietary selection pressure; 2) the effect of mutations in AGT on its folding, dimerization, and intracellular compartmentalization, and the downstream effects of AGT dysfunction on oxalate synthesis in primary hyperoxaluria type 1 (PH1). My key achievements have been as follows:- 1986. Discovered basic enzyme defect in PH1 (i.e. AGT deficiency). 1986-7. Carried out the first enzyme prenatal and postnatal diagnoses of PH1. 1986. Provided rationale and support for first successful liver transplantation as a form of enzyme replacement therapy in PH1. 1989. Discovered AGT peroxisome-to-mitochondrion mistargeting in PH1. 1990. Cloned the human AGT gene. 1990. Identified first mutations in AGT in PH1, including the mutation-polymorphism combination responsible for AGT mistargeting. 1991. Mapped the chromosomal location and intron-exon boundaries of the human AGT gene (AGXT). 1992-4. Cloned and functionally characterized the marmoset, rabbit and cat AGT genes, and proposed the role of dietary selection pressure in determining the peroxisomal and/or mitochondrial compartmentalization of mammalian AGT. 1995. Functionally identified AGT as a PTS1 protein. 1996. Determined the mechanism of AGT mistargeting in PH1. 1998. Cloned and functionally characterized the guinea pig AGT gene. 2000. Demonstrated that the AGT gene in primates evolved under positive (dietary?) selection pressure. 2000. Demonstrated the functional synergism between the common Pro11Leu polymorphism and many PH1-specific mutations in AGT. 2000-5. Demonstrated the atypical nature of the AGT PTS1. 2003. Determined the crystal structure of human AGT. 2003. Demonstrated the normalization of mutant AGT targeting in vitro using protein stabilization agents. 2004. Demonstrated that the AGT gene in Carnivora evolved under positive (dietary?) selection pressure. 2004. Demonstrated that the human population distribution of the AGT Pro11Leu polymorphism is compatible with its frequency being influenced by ancestral dietary history. 2005. Provided the first statistical validation of the evolutionary relationship between diet and AGT compartmentalization in mammals. 2005. Identified the ancillary peroxisomal targeting information (PTS1A) in human AGT. 2006. Constructed a set of singly, doubly and triply-transformed CHO cells suitable for the high-throughput screening of chemical chaperones as potential pharmacological treatments for PH1. My current research concentrates on development cell-based model systems suitable for the high-throughput screening of protein stabilising agents with potential for the pharmacological treatment of primary hyperoxaluria.

Research Activities

Alanine:glyoxylate aminotransferase (AGT) trafficking in evolution and genetic kidney stone disease

Education Description

Course organizer for:- BIOL2014 Cell Biology (year 2), BIOL3006 Molecular Cell Biology (year 3/4). Lecturer on:- BIOL2014 Cell Biology (year 2), BIOL3006 Molecular Cell Biology (year 3/4), BIOL3010 Molecular Evolution (year 3), BIOL3013 Genes to Disease to Therapy (year 3), PHOL2007 Principles of Cellular Control (year 2).

UCL Collaborators

External Collaborators

Publications

    2014

    • Miyata N, Steffen J, Johnson ME, Fargue S, Danpure CJ, Koehler CM (2014). Pharmacologic rescue of an enzyme-trafficking defect in primary hyperoxaluria 1.. Proc Natl Acad Sci U S A, , - . doi:10.1073/pnas.1408401111

    2013

    • Fargue S, Lewin J, Rumsby G, Danpure CJ (2013). Four of the most common mutations in primary hyperoxaluria type 1 unmask the cryptic mitochondrial targeting sequence of alanine:glyoxylate aminotransferase encoded by the polymorphic minor allele.. J Biol Chem, 288(4), 2475 - 2484. doi:10.1074/jbc.M112.432617
    • Fargue S, Rumsby G, Danpure CJ (2013). Multiple mechanisms of action of pyridoxine in primary hyperoxaluria type 1.. Biochim Biophys Acta, 1832(10), 1776 - 1783. doi:10.1016/j.bbadis.2013.04.010

    2012

    • Montioli R, Fargue S, Lewin J, Zamparelli C, Danpure CJ, Borri Voltattorni C, Cellini B (2012). The N-terminal extension is essential for the formation of the active dimeric structure of liver peroxisomal alanine:glyoxylate aminotransferase.. Int J Biochem Cell Biol, 44(3), 536 - 546. doi:10.1016/j.biocel.2011.12.007
    • Cochat P, Hulton SA, Acquaviva C, Danpure CJ, Daudon M, De Marchi M, Fargue S, Groothoff J, Harambat J, Hoppe B, Jamieson NV, Kemper MJ, Mandrile G, Marangella M, Picca S, Rumsby G, Salido E, Straub M, van Woerden CS, OxalEurope (2012). Primary hyperoxaluria Type 1: indications for screening and guidance for diagnosis and treatment.. Nephrol Dial Transplant, 27(5), 1729 - 1736. doi:10.1093/ndt/gfs078

    2010

    • Djordjevic S, Zhang X, Bartlam M, Ye S, Rao Z, Danpure CJ (2010). Structural implications of a G170R mutation of alanine:glyoxylate aminotransferase that is associated with peroxisome-to-mitochondrion mistargeting.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 66(Pt 3), 233 - 236. doi:10.1107/S1744309109054645

    2007

    • Danpure CJ, Rumsby G (2007). Proceedings of the 8th International Primary Hyperoxaluria Workshop, UCL-Institute of Child Health, 29-30 June 2007. UROL RES, 35(5), 253 - 254. doi:10.1007/s00240-007-0111-7

    2006

    • Danpure CJ (2006). Primary hyperoxaluria type 1: AGT mistargeting highlights the fundamental differences between the peroxisomal and mitochondrial protein import pathways.. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1763(12), 1776 - 1784. doi:10.1016/j.bbamcr.2006.08.021
    • Behnam JT, Williams E, Brink S, Rumsby G, Danpure CJ (2006). Reconstruction of human hepatocyte glyoxylate metabolic pathways in stably transformed Chinese hamster ovary cells.. Biochemical Journal, 394, 409 - 416. doi:10.1042/BJ20051397

    2005

    • Huber PAJ, Birdsey GM, Lumb MJ, Perkins TJ, Prowse DTR, Knight DR, Danpure CJ (2005). Peroxisomal import of human alanine:glyoxylate aminotransferase requires ancillary targeting information remote from it C-terminus.. Journal of Biological Chemistry, 280, 27111 - 27120. doi:10.1074/jbc.M502719200
    • Danpure CJ (2005). Molecular etiology of primary hyperoxaluria type 1: new directions for treatment.. American Journal of Nephrology, 25, 303 - 310. doi:10.1159/000086362
    • Birdsey GM, Lewin J, Holbrook JD, Simpson VR, Cunningham AA, Danpure CJ (2005). A comparative analysis of the evolutionary relationship between diet and enzyme targeting in bats, marsupials and other mammals.. Proceedings of the Royal Society B: Biological Sciences, 272(1565), 833 - 840. doi:10.1098/rspb.2004.3011
    • Danpure CJ (2005). The importance of basic research. Newsletter of the Oxalosis and Hyperoxaluria Foundation, , 4 - 5.
    • Danpure C (2005). Primary hyperoxaluria: from gene defects to designer drugs?. Nephrology Dialysis Transplantation, 20, 1525 - 1529. doi:10.1093/ndt/gfh923

    2004

    • Danpure CJ (2004). Twists and turns in the identification of the structural biochemistry of PH1.
    • Danpure CJ (2004). Molecular etiology and clinical management of primary hyperoxaluria type 1: new insights provided by knowledge of its cell and structural biology.. In Gohel MD, Au DW (Ed.), Kidney Stones: Inside & Out (pp. 216 - 219). : .
    • Danpure CJ, Rumsby G (2004). Molecular aetiology of primary hyperoxaluria and its implications for clinical management. Expert Reviews in Molecular Medicine, 6, 1 - 16. doi:10.1017/S1462399404007203
    • Birdsey GM, Lewin J, Cunningham AA, Bruford MW, Danpure CJ (2004). Differential enzyme targeting as an evolutionary adaptation to herbivory in Carnivora.. Molecular Biology and Evolution, 21(4), 632 - 646. doi:10.1093/molbev/msh054
    • Caldwell EF, Mayor L, Thomas MG, Danpure CJ (2004). Diet and the frequency of the alanine:glyoxylate aminotransferase Pro11Leu polymorphism in different human populations. Human Genetics, 115, 504 - 509. doi:10.1007/s00439-004-1191-x
    • Danpure CJ (2004). Variable peroxisomal and mitochondrial targeting of alanine:glyoxylate aminotransferase in mammalian evolution and human disease..
    • Danpure CJ (2004). Molecular aetiology of primary hyperoxaluria type 1. Nephron Experimental Nephrology, 98, 39 - 44. doi:10.1159/000080254
    • Danpure CJ (2004). Peroxisome-to-mitochondrion mistargeting of alanine:glyoxylate aminotransferase in primary hyperoxaluria type 1.

    2003

    • Zhang X, Roe SM, Hou Y, Bartlam M, Rao Z, Pearl LH, Danpure CJ (2003). Crystal structure of alanine:glyoxylate aminotransferase and the relationship between genotype and enzymatic phenotype in primary hyperoxaluria type 1. Journal of Molecular Biology, 331(3), 643 - 652. doi:10.1016/S0022-2836(03)00791-5
    • Van der Giezen M, Birdsey GM, Horner DS, Lucocq J, Dyal PL, Benchimol M, Danpure CJ, Embley TM (2003). Fungal hydrogenosomes contain mitochondrial heat shock proteins. Molecular Biology and Evolution, 20, 1051 - 1061. doi:10.1093/molbev/msg103
    • Danpure CJ, Lumb MJ, Birdsey GM, Zhang X (2003). Alanine:glyoxylate aminotransferase peroxisome-to-mitochondrion mistargeting in human hereditary kidney stone disease. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1647(1-2), 70 - 75. doi:10.1016/S1570-9639(03)00055-4
    • Watts RWE, Danpure CJ (2003). Disorders of oxalate metabolism. In Warrell DA, Cox TMF, J D (Ed.), Oxford Textbook of Medicine (pp. 134 - 138). : Oxford University Press.
    • Lumb MJ, Birdsey GM, Danpure CJ (2003). Correction of an enzyme trafficking defect in hereditary kidney stone disease in vitro. Biochemical Journal, 374, 79 - 87.

    2002

    • Minatogawa Y, Tone S, Allsop J, Purdue PE, Takada Y, Danpure CJ, Kido R (2002). A serine-to-phenylalanine substitution leads to loss of alanine:glyoxylate aminotransferase catalytic activity and immunoreactivity in a patient with primary hyperoxaluria type 1.. Human Molecular Genetics, 1(8), 643 - 644.
    • Holbrook JD, Danpure CJ (2002). Molecular basis for the dual mitochondrial and cytosolic localization of alanine:glyoxylate aminotransferase in amphibian liver cells. Journal of Biological Chemistry, 277, 2336 - 2344. doi:10.1074/jbc.M107047200
    • Sato M, Toné S, Ishikawa T, Purdue PE, Danpure CJ, Minatogawa Y (2002). Functional analysis of the 5¢-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1574(2), 205 - 209. doi:10.1016/S0167-4781(01)00285-8

    2001

    • Zhang X, Roe SM, Pearl LH, Danpure CJ (2001). Crystallization and preliminary crystallographic analysis of human alanine:glyoxylate aminotransferase and its polymorphic variants. Acta Crystallographica Section D, 57(12), 1936 - 1937. doi:10.1107/S0907444901017334
    • Danpure CJ (2001). Primary hyperoxaluria. In Scriver CR, Beaudet AL, Sly WS, Valle D, Childs B, Kinzler KW, Vogelstein B (Ed.), The Metabolic and Molecular Bases of Inherited Disease (pp. 3323 - 3367). : McGraw Hill.

    2000

    • Knott TG, Birdsey GM, Sinclair KE, Gallagher IM, Purdue PE, Danpure CJ (2000). The PTS1 receptor Pex5p and the peroxisomal import efficiency of alanine: glyoxylate aminotransferase. Biochemical Journal, 352(2), 409 - 418.
    • Knott TG, Birdsey GM, Sinclair KE, Gallagher IM, Purdue PE, Danpure CJ (2000). The peroxisomal targeting sequence type 1 receptor, Pex5p, and the peroxisomal import efficiency of alanine:glyoxylate aminotransferase.. Biochem J, 352 Pt 2, 409 - 418.
    • Holbrook JD, Birdsey GM, Yang Z, Bruford MF, Danpure CJ (2000). Molecular adaptation of alanine: glyoxylate aminotransferase targeting in primates. Molecular Biology and Evolution, 17, 387 - 400.
    • Danpure CJ (2000). Diet and enzyme trafficking. UCL Science, 14, 12 - 13.
    • Danpure CJ (2000). Genetic disorders and urolithiasis. In (Ed.), Urological Clinics of North America (pp. 287 - 299). : W.B. Saunders.
    • Holbrook JD, Danpure CJ (2000). Molecular evolution of alanine: glyoxylate aminotransferase intracellular targeting. In Iriarte A, Kagan HM, Martinez-Carrion M (Ed.), Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent proteins (pp. 117 - 122). : Birkhauser Verlag AG.
    • Lumb MJ, Danpure CJ (2000). Functional synergism between the most common polymorphism in human alanine: glyoxylate aminotransferase and four of the most common disease-causing mutations. Journal of Biological Chemistry, 275, 36415 - 36422. doi:10.1074/jbc.M006693200

    1999

    • Barratt TM, Danpure CJ (1999). Hyperoxaluria. In Barratt TM, Avner ED, Harmon WE (Ed.), Pediatric Nephrology (pp. 609 - 624). : Williams & Wilkins.
    • Danpure CJ (1999). Molecular basis of PH1: Implications for gene therapy. Nephrology Dialysis Transplantation, 14(11), 2784 - 2784.
    • Lumb MJ, Drake AF, Danpure CJ (1999). Effect of N-terminal à-helix formation on the dimerization and intracellular targeting of alanine:glyoxylate aminotransferase. Journal of Biological Chemistry, 274, 20587 - 20596.
    • Brink SC, Danpure CJ (1999). Protein import into peroxisomes: methods.. In (Ed.), Encyclopedia of Life Sciences (pp. - ). : John Wiley & Sons, Ltd. doi:10.1038/npg.els.0006101

    1998

    • Birdsey GM, Danpure CJ (1998). Evolution of alanine:glyoxylate aminotransferase intracellular targeting. Structural and functional analysis of the guinea pig gene. Biochemical Journal, 331, 49 - 60.
    • Danpure CJ (1998). The molecular basis of alanine:glyoxylate aminotransferase mistargeting, the most common single cause of primary hyperoxaluria type 1. Journal of Nephrology, 11, 8 - 12.

    1997

    • Danpure CJ (1997). Advances in the molecular biology of primary hyperoxaluria type 1 and their consequences for clinical management. Renal Stone Disease, Crystallization Process, Pathophysiology, metabolic Disorders and Prevention, , 35 - 40.
    • Hoppe B, Danpure CJ, Rumsby G, Fryer P, Jennings PR, Blau N, Schubiger G, Neuhaus T, Leumann E (1997). A vertical (pseudodominant) pattern of inheritance in the autosomal recessive disease primary hyperoxaluria type 1. Lack of relationship between genotype, enzymic phenotype and disease severity. AM J KID DIS, 29, 36 - 44.
    • Danpure CJ (1997). Variable peroxisomal and mitochondrial targeting of alanine: glyoxylate aminotransferase in mammalian evolution and disease. Bioessays, 19, 317 - 326.
    • Leiper JM, Danpure CJ (1997). A unique molecular basis for enzyme mistargeting in primary hyperoxaluria type 1. CLIN CHIM ACTA, 266, 39 - 50.
    • Danpure CJ, Purdue PE (1997). Primary hyperoxaluria. In Scriver CR, Beaudet AL, Sly WS, Valle D (Ed.), (pp. - ). : McGraw-Hill.

    1996

    • Leiper JM, Danpure CJ (1996). The role of dimerization of alanine:glyoxylate aminotransferase 1 in its peroxisomal and mitochondrial import.. Ann N Y Acad Sci, 804, 765 - 767.
    • Oatey PB, Lumb MJ, Jennings PR, Danpure CJ (1996). Context dependency of the PTS1 motif in human alanine: glyoxylate aminotransferase 1.. Ann N Y Acad Sci, 804, 652 - 653.
    • Danpure CJ, Jennings PR, Leiper JM, Lumb MJ, Oatey PB (1996). Targeting of alanine: glyoxylate aminotransferase in normal individuals and its mistargeting in patients with primary hyperoxaluria type 1. Annals of the New York Academy of Sciences, 804, 477 - 490. doi:10.1111/j.1749-6632.1996.tb18638.x
    • Danpure CJ, Smith LH (1996). The primary hyperoxalurias. In Coe FL, Favus MJ, Pak CY, Parks JH, Preminger GM (Ed.), Kidney Stones: Medical and Surgical Management (pp. 859 - 881). : Lippincott-Raven.
    • Danpure CJ (1996). Targeting and import of peroxisomal proteins. In Hurtley SM (Ed.), Protein Targeting (pp. 63 - 100). : Oxford University Press.
    • Danpure CJ (1996). Ask the expert. PEDIATR NEPHROL, 10(2), 190 - 190.
    • Danpure CJ (1996). What are the present possibilities for the prenatal diagnosis of primary hyperoxaluria?. Pediatric Nephrology, 10, 190 - .
    • Oatey PB, Lumb MJ, Danpure CJ (1996). Molecular basis of the variable peroxisomal and mitochondrial targeting of alanine:glyoxylate aminotransferase.. MOL BIOL CELL, 7, 2878 - 2878.
    • Leiper JM, Oatey PB, Danpure CJ (1996). Inhibition of alanine:glyoxylate aminotransferase 1 dimerization is a prerequisite for its peroxisome-to-mitochondrion mistargeting in primary hyperoxaluria type 1.. J Cell Biol, 135(4), 939 - 951.
    • Oatey P, B L, M J D, C J (1996). Molecular basis of the variable mitochondrial and peroxisomal localisation of alanine-glyoxylate aminotransferase. European Journal of Biochemistry, 241(2), 374 - 385. doi:10.1111/j.1432-1033.1996.00374.x
    • Hoppe B, Danpure CJ, Rumsby G, Fryer P, Jennings PR, Blau N, Neuhaus T, Leumann E (1996). Vertical (pseudodominant) inheritance in primary hyperoxaluria type I (PH I): Discrepancy between disease phenotype and genotype.. J AM SOC NEPHROL, 7(9), A1827 - A1827.
    • Danpure CJ, Rumsby G (1996). Strategies for the prenatal diagnosis of primary hyperoxaluria type 1.. Prenatal Diagnosis, 16(7), 587 - 598.

    1995

    • Danpure CJ (1995). Advances in the enzymology and molecular genetics of primary hyperoxaluria type 1. Prospects for gene therapy. Nephrology Dialysis Transplantation, 10 Suppl(8), 24 - 29.
    • Danpure CJ (1995). How can the products of a single gene be localized to more than one intracellular compartment?. Trends in Cell Biology, 5(6), 230 - 238. doi:10.1016/S0962-8924(00)89016-9
    • Motley A, Lumb MJ, Oatey PB, Jennings PR, De Zoysa PA, Wanders RJ, Tabak HF, Danpure CJ (1995). Mammalian alanine/glyoxylate aminotransferase 1 is imported into peroxisomes via the PTS1 translocation pathway. Increased degeneracy and context specificity of the mammalian PTS1 motif and implications for the peroxisome-to-mitochondrion mistargeting of AGT in primary hyperoxaluria type 1.. The Journal of Cell Biology, 131(1), 95 - 109.
    • Danpure CJ, Rumsby G (1995). Enzymology and molecular genetics of primary hyperoxaluria type 1. Consequences for clinical management. In Khan SR (Ed.), Calcium Oxalate in Biological Systems (pp. 189 - 205). : CRC Press Inc..
    • Danpure CJ, Purdue PE (1995). Primary hyperoxaluria. In (Ed.), The Metabolic and Molecular Bases of Inherited Disease (pp. 2385 - 2424). : .

    1994

    • Danpure CJ (1994). Molecular and cell biology of primary hyperoxaluria type 1.. Clin Investig, 72(9), 725 - 727.
    • Danpure CJ, Fryer P, Jennings PR, Allsop J, Griffiths S, Cunningham A (1994). Evolution of alanine:glyoxylate aminotransferase 1 peroxisomal and mitochondrial targeting. A survey of its subcellular distribution in the livers of various representatives of the classes Mammalia, Aves and Amphibia. European Journal of Cell Biology, 64(2), 295 - 313.
    • Danpure CJ, Birdsey GM, Rumsby G, Lumb MJ, Purdue PE, Allsop J (1994). Molecular characterization and clinical use of a polymorphic tandem repeat in an intron of the human alanine:glyoxylate aminotransferase gene. Human Genetics, 94, 55 - 64.
    • HOPPE B, DANPURE CJ, BRAEGGER C, COLOMBI A, SCHUBIGER G, LEUMANN E (1994). APPARENT AUTOSOMAL-DOMINANT INHERITANCE IN PRIMARY HYPEROXALURIA TYPE-I. KIDNEY INT, 45(6), 1783 - 1783.
    • Lumb MJ, Purdue PE, Danpure CJ (1994). Molecular evolution of alanine/glyoxylate aminotransferase 1 intracellular targeting. Analysis of the feline gene.. European Journal of Biochemistry, 221(1), 53 - 62. doi:10.1111/j.1432-1033.1994.tb18714.x
    • Danpure CJ, Jennings PR, Fryer P, Purdue PE, Allsop J (1994). Primary hyperoxaluria type 1: genotypic and phenotypic heterogeneity. Journal of Inherited Metabolic Disease, 17, 487 - 499.
    • Danpure CJ, Fryer P, Griffiths S, Guttridge KM, Jennings PR, Allsop J, Moser AB, Naidu S, Moser HW, MacCollin M, DeVivo DC (1994). Cytosolic compartmentalization of hepatic alanine:glyoxylate aminotransferase in patients with aberrant peroxisomal biogenesis and its effect on oxalate metabolism. Journal of Inherited Metabolic Disease, 17(1), 27 - 40.
    • Barratt TM, Danpure CJ (1994). Hyperoxaluria.. In Holliday MA, Barratt TM, Avner ED (Ed.), Pediatric Nephrology (pp. 557 - 572). : Williams & Wilkins.

    1993

    • de Zoysa PA, Danpure CJ (1993). Possible evolutionary relationship between mammalian alanine:glyoxylate aminotransferase 1 and the 42-kD subunit of cyanobacterial soluble hydrogenase. Molecular Biology and Evolution, 10(3), 704 - 706.
    • Danpure CJ, Purdue PE, Fryer P, Griffiths S, Allsop J, Lumb MJ, Guttridge KM, Jennings PR, Scheinman JI, Mauer SM, Davidson NO (1993). Enzymological and mutational analysis of a complex primary hyperoxaluria type 1 phenotype involving alanine:glyoxylate aminotransferase peroxisome-to-mitochondrion mistargeting and intraperoxisomal aggregation. , 53(2), 417 - 432.
    • Danpure CJ (1993). Primary hyperoxaluria type 1 and peroxisome-to-mitochondrion mistargeting of alanine:glyoxylate aminotransferase.. Biochimie, 75(3-4), 309 - 315. doi:10.1016/0300-9084(93)90091-6

    1992

    • Illum N, Lavard L, Danpure CJ, Horn T, Aerenlund Jensen H, Skovby F (1992). Primary hyperoxaluria type 1: clinical manifestations in infancy and prenatal diagnosis. Child Nephrology and Urology, 12(4), 225 - 227.
    • Purdue PE, Lumb MJ, Allsop J, Minatogawa Y, Danpure CJ (1992). A glycine-to-glutamate substitution abolishes alanine:glyoxylate aminotransferase catalytic activity in a subset of patients with primary hyperoxaluria type 1. Genomics, 13(1), 215 - 218. doi:10.1016/0888-7543(92)90225-H
    • Katz A, Freese D, Danpure CJ, Scheinman JI, Mauer SM (1992). Success of kidney transplantation in oxalosis is unrelated to residual hepatic enzyme activity.. Kidney International, 42(6), 1408 - 1411.
    • Rumsby G, Jones R, Danpure CJ, Samuell CT (1992). TaqI polymorphism at the alanine:glyoxylate aminotransferase (AGXT) gene locus. Human Molecular Genetics, 1(5), 350 - .
    • Purdue PE, Lumb MJ, Danpure CJ (1992). Molecular evolution of alanine/glyoxylate aminotransferase 1 intracellular targeting. Analysis of the marmoset and rabbit genes. European Journal of Biochemistry, 207(2), 757 - 766. doi:10.1111/j.1432-1033.1992.tb17106.x
    • Danpure CJ (1992). Molecular aetiology and pathogenesis of primary hyperoxaluria: implications for clinical management. In Raine AEG (Ed.), Advanced Renal Medicine (pp. 175 - 182). : Oxford University Press.

    1991

    • DANPURE CJ, PURDUE PE (1991). MOLECULAR-GENETICS AND ENZYMOLOGY OF PRIMARY HYPEROXALURIA TYPE-I. AM J HUM GENET, 49(4), 27 - 27.
    • WATTS RWE, DANPURE CJ, DEPAUW L, TOUSSAINT C (1991). COMBINED LIVER KIDNEY AND ISOLATED LIVER TRANSPLANTATIONS FOR PRIMARY HYPEROXALURIA TYPE-1 - THE EUROPEAN EXPERIENCE. NEPHROL DIAL TRANSPL, 6(7), 502 - 511.
    • De Pauw L, Watts RW, Danpure CJ, Toussaint C (1991). [Which transplantation strategies in primary hyperoxaluria type 1?].. Nephrologie, 12(3), 147 - 149.
    • Danpure CJ (1991). Scientific rationale for hepatorenal transplantation in primary hyperoxaluria type 1.. In Touraine JL (Ed.), Transplantation and Clinical Immunology (pp. 91 - 98). : Excerpta Medica.
    • Watts RW, Danpure CJ, De Pauw L, Toussaint C (1991). Combined liver-kidney and isolated liver transplantations for primary hyperoxaluria type 1: the European experience. The European Study Group on Transplantation in Hyperoxaluria Type 1. Nephrology Dialysis Transplantation, 6(7), 502 - 511.
    • Purdue PE, Allsop J, Isaya G, Rosenberg LE, Danpure CJ (1991). Mistargeting of peroxisomal L-alanine:glyoxylate aminotransferase to mitochondria in primary hyperoxaluria patients depends upon activation of a cryptic mitochondrial targeting sequence by a point mutation. Proceedings of the National Academy of Sciences of the United States of America, 88(23), 10900 - 10904.
    • Purdue PE, Lumb MJ, Allsop J, Danpure CJ (1991). An intronic duplication in the alanine: glyoxylate aminotransferase gene facilitates identification of mutations in compound heterozygote patients with primary hyperoxaluria type 1. Human Genetics, 87(4), 394 - 396.
    • Purdue PE, Lumb MJ, Fox M, Griffo G, Hamon-Benais C, Povey S, Danpure CJ (1991). Characterization and chromosomal mapping of a genomic clone encoding human alanine:glyoxylate aminotransferase.. Genomics, 10(1), 34 - 42. doi:10.1016/0888-7543(91)90481-S
    • Danpure CJ (1991). Molecular and clinical heterogeneity in primary hyperoxaluria type 1. American Journal of Kidney Diseases, 17(4), 366 - 369.
    • Watts RW, Morgan SH, Danpure CJ, Purkiss P, Calne RY, Rolles K, Baker LR, Mansell MA, Smith LH, Merion RM (1991). Combined hepatic and renal transplantation in primary hyperoxaluria type I: clinical report of nine cases. The American Journal of Medicine, 90(2), 179 - 188.
    • Danpure CJ, Jennings PR, Jansen JH (1991). Enzymological characterization of a putative canine analogue of primary hyperoxaluria type 1.. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1096(2), 134 - 138.
    • De Pauw L, Watts RW, Danpure CJ, Toussaint C (1991). Quelles strategies de transplantation dans l'yperoxalurie primitive de type 1?. Néphrologie, 12(3), 147 - 149.

    1990

    • Purdue PE, Takada Y, Danpure CJ (1990). Identification of mutations associated with peroxisome-to-mitochondrion mistargeting of alanine/glyoxylate aminotransferase in primary hyperoxaluria type 1.. J Cell Biol, 111(6 Pt 1), 2341 - 2351.
    • Danpure CJ, Guttridge KM, Fryer P, Jennings PR, Allsop J, Purdue PE (1990). Subcellular distribution of hepatic alanine:glyoxylate aminotransferase in various mammalian species.. J Cell Sci, 97 ( Pt 4), 669 - 678.

    1989

    • Cooper PJ, Danpure CJ, Simpson KJ (1989). Peroxisomal and mitochondrial proliferation and increased alanine: glyoxylate aminotransferase activity in human liver after chlorpromazine-induced cholestasis.. Biochem Soc Trans, 17(6), 1071 - 1072.
    • Danpure CJ, Cooper PJ, Jennings PR, Wise PJ, Penketh RJ, Rodeck CH (1989). Enzymatic prenatal diagnosis of primary hyperoxaluria type 1: potential and limitations.. J Inherit Metab Dis, 12 Suppl 2, 286 - 288.
    • McKerrell RE, Blakemore WF, Heath MF, Plumb J, Bennett MJ, Pollitt RJ, Danpure CJ (1989). Primary hyperoxaluria (L-glyceric aciduria) in the cat: a newly recognised inherited disease.. Vet Rec, 125(2), 31 - 34.

    1988

    • COOPER PJ, DANPURE CJ, WISE PJ, GUTTRIDGE KM (1988). IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF ALANINE GLYOXYLATE AMINOTRANSFERASE IN NORMAL HUMAN-LIVER AND TYPE-1 HYPEROXALURIC LIVER. BIOCHEM SOC T, 16(4), 627 - 628.
    • MISTRY J, DANPURE CJ, CHALMERS RA (1988). HEPATIC D-GLYCERATE DEHYDROGENASE AND GLYOXYLATE REDUCTASE DEFICIENCY IN PRIMARY HYPEROXALURIA TYPE-2. BIOCHEM SOC T, 16(4), 626 - 627.
    • Danpure CJ, Jennings PR, Penketh RJ, Wise PJ, Rodeck CH (1988). Prenatal exclusion of primary hyperoxaluria type 1.. Lancet, 1(8581), 367 - .
    • Danpure CJ, Jennings PR (1988). Enzymatic heterogeneity in primary hyperoxaluria type 1 (hepatic peroxisomal alanine: glyoxylate aminotransferase deficiency).. J Inherit Metab Dis, 11 Suppl 2, 205 - 207.
    • Thompson GN, Purkiss P, Danpure CJ (1988). The subcellular metabolism of glyoxylate in primary hyperoxaluria type 1: the relationship between glycine production and oxalate overproduction.. J Inherit Metab Dis, 11 Suppl 2, 212 - 214.

    1987

    • DANPURE CJ, JENNINGS PR, WATTS RWE (1987). PRIMARY HYPEROXALURIA TYPE-1 AND HEPATIC ALANINE - GLYOXYLATE AMINOTRANSFERASE, A STUDY OF 5 CASES. CLIN SCI, 72, P23 - P23.
    • Watts RWE, Calne RY, Rolles K, Danpure CJ, MorganS H M, M A W, R P, P (1987). Successful treatment of primary hyperoxaluria type 1 by combined hepatic and renal transplantation. Lancet 2, 474-475 (1987).. The Lancet, 2(8557), 474 - 475. doi:10.1016/S0140-6736(87)91791-0
    • Watts RW, Calne RY, Rolles K, Danpure CJ, Morgan SH, Mansell MA, Williams R, Purkiss P (1987). Successful treatment of primary hyperoxaluria type I by combined hepatic and renal transplantation.. Lancet, 2(8557), 474 - 475.
    • Wise PJ, Danpure CJ, Jennings PR (1987). Immunological heterogeneity of hepatic alanine:glyoxylate aminotransferase in primary hyperoxaluria type 1.. FEBS Lett, 222(1), 17 - 20.
    • Allsop J, Jennings PR, Danpure CJ (1987). A new micro-assay for human liver alanine: glyoxylate aminotransferase.. Clin Chim Acta, 170(2-3), 187 - 193.

    1986

    • DANPURE CJ, JENNINGS PR (1986). ALANINE - GLYOXYLATE AND SERINE - PYRUVATE AMINOTRANSFERASES IN PRIMARY HYPEROXALURIA TYPE-1. BIOCHEM SOC T, 14(6), 1059 - 1060.
    • DANPURE CJ, JENNINGS PR (1986). DEFICIENCY OF PEROXISOMAL ALANINE - GLYOXYLATE AMINOTRANSFERASE IN PRIMARY HYPEROXALURIA TYPE-I. EUR J CELL BIOL, 41, 11 - 11.
    • Danpure CJ, Jennings PR, Fyfe DA (1986). Further studies on the effect of chloroquine on the uptake, metabolism and intracellular translocation of [35S]cystine in cystinotic fibroblasts.. Biochim Biophys Acta, 885(3), 256 - 265.

    1985

    • DANPURE CJ, FYFE DA, JENNINGS PR (1985). EXTRACELLULAR PH AND THE UPTAKE OF CYSTINE BY NORMAL AND CYSTINOTIC FIBROBLASTS. BIOCHEM SOC T, 13(4), 687 - 688.
    • DANPURE CJ, PURKISS P, JENNINGS PR, WATTS RWE (1985). THE SUBCELLULAR-DISTRIBUTION OF 2-OXO-GLUTARATE - GLYOXYLATE CARBOLIGASE IN PRIMARY HYPEROXALURIA TYPE-I. CLIN SCI, 69, P45 - P45.

    1984

    • Michelakakis H, Danpure CJ (1984). The effect of cytochalasin B on the release of lysosomal enzymes and intra-lysosomally-stored polyvinylpyrrolidone in the isolated perfused rat liver.. Biochem Pharmacol, 33(13), 2047 - 2053.
    • DANPURE CJ, JENNINGS PR, FYFE DA, HALSALL S, PETERS TJ (1984). MECHANISM OF CHLOROQUINE-INDUCED INHIBITION AND STIMULATION OF THE LABELING OF THE INTRALYSOSOMAL CYSTINE POOL IN CYSTINOTIC FIBROBLASTS. BIOCHEM SOC T, 12(6), 1037 - 1039.
    • GAVRIAS V, DANPURE CJ (1984). THE EFFECT OF MICROTUBULE AND MICROFILAMENT DISRUPTION ON THE SECRETION OF FUCOSE-CONTAINING AND MANNOSE-CONTAINING GLYCOPROTEINS FROM NORMAL AND CYSTIC-FIBROSIS FIBROBLASTS. BIOCHEM SOC T, 12(2), 287 - 288.
    • DANPURE CJ, JENNINGS PR, HALSALL S, PETERS TJ (1984). MECHANISM OF UPTAKE AND INTRACELLULAR TRANSLOCATION OF [S-35] CYSTINE IN CYSTINOTIC FIBROBLASTS. BIOCHEM SOC T, 12(5), 857 - 857.

    1982

    • Danpure CJ (1982). Induced exocytosis of macromolecular storage product as a potential treatment for lysosomal-storage-diseases. In Crawfurd MA, Gibbs DA, Watts RWE (Ed.), Advances in the Treatment of Inborn Errors of Metabolism (pp. 259 - 271). : Wiley.

    1980

    • MICHELAKAKIS H, DANPURE CJ (1980). EFFECT OF CYTOCHALASIN-B ON THE RELEASE OF LYSOSOMAL-ENZYMES AND PRELOADED I-125-PVP FROM ISOLATED PERFUSED-RAT-LIVER. EUR J CELL BIOL, 22(1), 200 - 200.
    • Danpure CJ, Fyfe DA (1980). The effect of chloroquine on the intralysosomal accumulation of cystine in cystinotic and normal fibroblasts.. Biochem Soc Trans, 8(5), 571 - .
    • Michelakakis H, Danpure CJ (1980). The uptake and release of lysosomal enzymes by isolated perfused rat liver.. Biochem Soc Trans, 8(5), 569 - 570.
    • DANPURE CJ, ENGLISH NR (1980). THE EFFECT OF CYTOCHALASIN-B AND CHLOROQUINE ON THE SECRETION OF LYSOSOMAL-ENZYMES AND MUCOPOLYSACCHARIDES FROM NORMAL AND HURLERS DISEASE FIBROBLASTS. EUR J CELL BIOL, 22(1), 345 - 345.
    • MICHELAKAKIS H, DANPURE CJ (1980). EFFECT OF CYTOCHALASIN-B ON THE RELEASE OF LYSOSOMAL-ENZYMES FROM ISOLATED PERFUSED-RAT-LIVER. CELL BIOL INT REP, 4(8), 773 - 773.

    1979

    • Michelakakis H, Danpure CJ (1979). The effect of cytochalasin B on the release of lysosomal enzymes from isolated rat hepatocytes [proceedings].. Biochem Soc Trans, 7(6), 1283 - 1285.

    1977

    • Danpure CJ (1977). 'Free' plasma gold in rheumatoid patients undergoing chrysotherapy [proceedings].. J Physiol, 267(1), 25P - 26P.
    • Ward RJ, Danpure CJ, Fyfe DA (1977). Comparison between electrothermal-atomic-absorption-spectrometry and neutron-activation-analysis in the determination of gold in plasma and plasma fractions of rheumatoid patients receiving chrysotherapy. In Brown SS (Ed.), Clinical Chemistry and Chemical Toxicology of Metals (pp. 301 - 304). : Elsevier/North-Holland.
    • Lawson KJ, Danpure CJ, Fye DA (1977). The uptake and subcellular distribution of gold in rat liver cells after in vivo administration of sodium aurothiomalate.. Biochem Pharmacol, 26(24), 2417 - 2426.
    • Danpure CJ, Lawson KJ (1977). Interaction of aurothiomalate and cystine [proceedings].. Biochem Soc Trans, 5(5), 1366 - 1368.
    • DANPURE CJ (1977). FREE PLASMA GOLD IN RHEUMATOID PATIENTS UNDERGOING CHRYSOTHERAPY. J PHYSIOL-LONDON, 267(1), P25 - P26.
    • DANPURE CJ, LAWSON KJ (1977). INTERACTION OF AUROTHIOMALATE AND CYSTINE. BIOCHEM SOC T, 5(5), 1366 - 1368.

    1976

    • Danpure CJ (1976). Interaction of aurothiomalate with human serum albumin in vitro.. Biochem Soc Trans, 4(1), 161 - 163.
    • Howell A, Cooksley WG, Danpure CJ (1976). Release of colony-stimulating activity from the isolated perfused rat liver.. Clin Sci Mol Med, 50(6), 539 - 544.
    • Danpure CJ, Fyfe DA (1976). Inability of organomercurials to inhibit the reaction between aurothiomalate and thiols.. Biochem Soc Trans, 4(4), 631 - 633.

    1974

    • Danpure CJ, Taylor DM (1974). The effect of internally deposited plutonium-239 on the lysosomes of rat liver.. Radiat Res, 59(3), 679 - 692.
    • Howell A, Cooksley WG, Danpure CJ (1974). Proceedings: Bone marrow colony stimulating activity from the isolated perfused rat liver.. Clin Sci Mol Med, 46(2), 17P - .
    • HOWELL A, COOKSLEY WG, DANPURE CJ (1974). BONE-MARROW COLONY STIMULATING ACTIVITY FROM ISOLATED PERFUSED RAT-LIVER. CLIN SCI MOL MED, 46(2), P17 - P17.
    • LAWSON KJ, DANPURE CJ, FYFE DA, WATTS RWE (1974). SUBCELLULAR-DISTRIBUTION OF AUROTHIOMALATE IN RAT-LIVER INVIVO. BIOCHEM SOC T, 2(5), 896 - 899.
    • DANPURE CJ (1974). BINDING OF AUROTHIOMALATE TO SERUM-PROTEINS INVITRO. BIOCHEM SOC T, 2(5), 899 - 901.

    1971

    • Popplewell DS, Boocock G, Taylor DM, Danpure CJ (1971). The subcellular distribution of americium and curium in rat liver. In (Ed.), Radiation Protection Problems Relating to Transuranium Elements (pp. 205 - 222). : CID.

    1970

    • TAYLOR DM, DANPURE CJ (1970). MECHANISM OF DEPOSITION OF PLUTONIUM AND AMERICIUM IN TISSUES. HEALTH PHYS, 19(1), 123 - &.

    1969

    • Taylor DM, Danpure CJ (1969). Lysosomal uptake of actinide elements.. Biochem J, 115(5), 53P - .
    • TAYLOR DM, DANPURE CJ (1969). LYSOSOMAL UPTAKE OF ACTINIDE ELEMENTS. BIOCHEM J, 115(5), P53 - &.

    • Danpure CJ, Milliner DS (). Hereditary disorders of oxalate metabolism - the primary hyperoxalurias. In Warrell DA, Cox TM, Firth JD (Ed.), Oxford Textbook of Medicine, 5th edition (pp. - ). : Oxford University Press.
    • Danpure C (). Oxalate metabolism and the primary hyperoxalurias. In Rao NP, Preminger GMK, J (Ed.), Textbook of Urinary Stone Disease (pp. - ). : .