Dr Yasu Takeuchi

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Personal Profile

Name: Yasu Takeuchi Email: y.takeuchi@ucl.ac.uk
Title: Dr Tel: 2031082144
Department: Div of Infection & Immunity Fax: 2031082119
Position: Reader in Molecular Virology Address: Wohl Virion Centre, Cruciform Building, Gower Street, London, WC1E  6BT
Research Domain: Basic Life Sciences, Infection, Immunology & Inflammation, Personalised Medicine Web Page: Personal Web Page

Profile

Research Description

I studied Biochemistry at the University of Tokyo where I obtained an MSc on nucleotide physico-chemistry and a PhD on the molecular biology of small nuclear RNAs. I started his research on retroviruses upon appointment as a junior lecturer at Gunma University, Japan in 1986, and have been based in the UK since 1990, initially at the Institute of Cancer Research and then at UCL, where he is Reader in Molecular Virology.

I have worked on human viruses, HIV and HTLV and mammalian gammaretroviruses including porcine endogenous retroviruses (PERV). My research in Virology has concerned several aspects including early steps of infection and envelope-receptor interaction, evolution, oncogenesis and cell immortalisation and intrinsic antiviral immunity. The applied side of my research has been focused on the use of retroviruses as vectors for gene therapy and gene transfer and on zoonotic infections in xenotransplantation.

Vector packaging cells.
I have contributed to construction of stable packaging cell lines for MLV and HIV vectors. First I jointly with Francois-Loic Cosset developed packaging cell lines producing high-titre, complement resistant MLV vectors (patent applied and published in 1995). Complement resistance is important for in vivo use of the vector. These cell lines were deposited at ECACC and distributed internationally. Subsequently using the same expression system for MLV proteins, my lab developed suspension MLV packaging cell lines (2001). More recently as a joint project with Collins lab we developed stable HIV vector packaging lines (2003). Again these cells are deposited at ECACC and internationally circulated. We are currently working on further improvement of this system for production of clinical grade vectors with improved safety, funded by a MRC grant (PI: Mary Collins).

Safety in gene therapy
Safety problems in gene therapy concerning retroviral insertion and oncogenesis have been highlighted by recent incidents in clinical trials, where children developed leukemia after treatment for severe combined immunodeficiency with retroviral vectors (Hacein-Bey-Abina et al, Science 2003, 302: 415-419). In collaboration with Mary Collins, we have develped methods to measure retroviral insertion mutagenesis in cell culture and now are screening for safer vectors with lower mutagenic potential.

Potential risk of zoonoses in xenotransplantation.
Initially led by Robin Weiss we identified endogenous retroviruses in pigs that infect human cells as a major safety threat in xenotransplantation (1997). This seminal finding was followed by our and others' studies on PERV and called for cautious approaches to development of clinical xenotransplantation in the field. As for our PERV virology studies, we identified three subgroups of PERV with distinct receptor usage (1997-1998), an effective anti-viral drug (2001) and receptor genes for PERV-A (2003). These were followed by studies on complement sensitivity of viruses produced normal and genetically modified pig cells (2003) and PERV recombination (2004). These studies have contributed to the understanding of the nature of virological function and genome organisation of PERV and help assess the risk of potential zoonosis of PERV. Studies on structure-function of PERRV-A receptors and their physiological roles are currently ongoing.

I am currently involved in an EU FP6 consortium XENOME (Engineering of porcine genome for xenotransplantation studies in primates: step towards clinical application, www.xenome.eu), as a work package leader on safety. Projects include integration site analyses of PERV in pigs, PERV epigenetics, transgenic strategies for PERV inhibition, virus discovery by microarray (with Paul Kellam) and hepatitis E virus study (with Tim Harrison).

Research Activities

Humoral immunity to HIV, emerging infections

Retrovirus in gene therapy and xenotransplantation

Education Description

Co-organising Virl3001 course, responsible for the tutorials since 2007. Organising an MSc course on HIV starting in 2009.

UCL Collaborators

Dr Ariberto Fassati; Prof Robin Weiss; Prof Mary Collins; Prof Chris Boshoff; Prof Greg Towers

External Collaborators

Publications

    2014

    • Shimizu A, Nakatani Y, Nakamura T, Jinno-Oue A, Ishikawa O, Boeke JD, Takeuchi Y, Hoshino H (2014). Characterisation of cytoplasmic DNA complementary to non-retroviral RNA viruses in human cells.. Sci Rep, 4, 5074 - . doi:10.1038/srep05074
    • Busby SA, Robb A, Lang S, Takeuchi Y, Vesely P, Scobie L (2014). Antibiotic susceptibility and resistance of Staphylococcus aureus isolated from fresh porcine skin xenografts: Risk to recipients with thermal injury.. Burns, 40(2), 288 - 294. doi:10.1016/j.burns.2013.06.006

    2013

    • Winkler S, Wheeler J, Werling N, Dolman C, Thorpe R, Collins M, Takeuchi Y, Zhao Y (2013). Comparative analysis of cellular proteins in stably and transiently produced lentiviral vectors.
    • Cuccurullo EC, Pistello M, Takeuchi Y, Miyazawa T, Massimo P (2013). Functional analyses of glycosylated gag molecules derived from different gammaretroviruses. RETROVIROLOGY, 10, S16 - S16.
    • Knight S, Sanber K, Stephen S, Bailey R, Escors D, Santilli G, Thrasher A, Collins M, Takeuchi Y (2013). A clinical-grade constitutive packaging cell line for the production of self-inactivating lentiviral vectors.
    • Scobie L, Padler-Karavani V, Bas-Bernardet S, Crossan C, Blaha J, Matouskova M, Hector R, Cozzi E, Vanhove B, Charreau B, Blancho G, Bourdais L, Tallacchini M, Ribes J, Yu H, Chen X, Kracikova J, Broz L, Hejnar J, Vesely P, Takeuchi Y, Varki A, Soulillou J-P (2013). Long-term IgG response to porcine Neu5Gc-antigens in burn patients treated with porcine skin xenografts.
    • Wali R, Crossan C, Takeuchi Y, Blanchard Y, Scobie L (2013). Restriction of PERV subtypes by ZFP809 and TRIM28.
    • Scobie L, Padler-Karavani V, Le Bas-Bernardet S, Crossan C, Blaha J, Matouskova M, Hector RD, Cozzi E, Banhove B, Charreau B, Blancho G, Bourdais L, Tallacchini M, Ribes JM, Yu H, Chen X, Kracikova J, Broz L, Hejnar J, Vesely P, Takeuchi Y, Varki A, Soulillou JP (2013). Long-term IgG response to porcine Neu5Gc-antigens without transmission of PERV in burn patients treated with porcine skin xenografts. Journal of Immunology, , - .
    • Busby S-A, Crossan C, Godwin J, Petersen B, Galli C, Cozzi E, Takeuchi Y, Scobie L (2013). Suggestions for the diagnosis and elimination of hepatitis E virus in pigs used for xenotransplantation. XENOTRANSPLANTATION, 20(3), 188 - 192. doi:10.1111/xen.12038
    • Knight S, Collins M, Takeuchi Y (2013). Insertional mutagenesis by retroviral vectors: current concepts and methods of analysis.. Curr Gene Ther, 13(3), 211 - 227.
    • Matouskova M, Vesely P, Daniel P, Mattiuzzo G, Hector RD, Scobie L, Takeuchi Y, Hejnar J (2013). Role of DNA Methylation in Expression and Transmission of Porcine Endogenous Retroviruses. JOURNAL OF VIROLOGY, 87(22), 12110 - 12120. doi:10.1128/JVI.03262-12
    • Scobie L, Crossan C, Busby S-A, Vadori M, Seveso M, Cozzi E, Bas-Bernadet S, Blancho G, Takeuchi Y (2013). No apparent transmission of HEV via kidney xenotransplantation in a pig to primate model, but potential reactivation of pre-existing HEV infection.

    2012

    • Montiel-Equihua CA, Zhang L, Knight S, Saadeh H, Scholz S, Carmo M, Alonso-Ferrero ME, Blundell MP, Monkeviciute A, Schulz R, Collins M, Takeuchi Y, Schmidt M, Fairbanks L, Antoniou M, Thrasher AJ, Gaspar HB (2012). Development of a gene expression system for enhanced erythroid expression.
    • Zhang F, Knight S, Collins M, Takeuchi Y, Antoniou M, Thrasher AJ (2012). Improved safety of Lentiviral vectors containing silencing resistant A2UCOE regulatory elements.
    • Groenen MAM, Archibald AL, Uenishi H, Tuggle CK, Takeuchi Y, Rothschild MF, Rogel-Gaillard C, Park C, Milan D, Megens H-J, Li S, Larkin DM, Kim H, Frantz LAF, Caccamo M, Ahn H, Aken BL, Anselmo A, Anthon C, Auvil L, Badaoui B, Beattie CW, Bendixen C, Berman D, Blecha F, Blomberg J, Bolund L, Bosse M, Botti S, Bujie Z, Bystrom M, Capitanu B, Carvalho-Silva D, Chardon P, Chen C, Cheng R, Choi S-H, Chow W, Clark RC, Clee C, Crooijmans RPMA, Dawson HD, Dehais P, De Sapio F, Dibbits B, Drou N, Du Z-Q, Eversole K, Fadista J, Fairley S, Faraut T, Faulkner GJ, Fowler KE, Fredholm M, Fritz E, Gilbert JGR, Giuffra E, Gorodkin J, Griffin DK, Harrow JL, Hayward A, Howe K, Hu Z-L, Humphray SJ, Hunt T, Hornshoj H, Jeon J-T, Jern P, Jones M, Jurka J, Kanamori H, Kapetanovic R, Kim J, Kim J-H, Kim K-W, Kim T-H, Larson G, Lee K, Lee K-T, Leggett R, Lewin HA, Li Y, Liu W, Loveland JE, Lu Y, Lunney JK, Ma J, Madsen O, Mann K, Matthews L, McLaren S, Morozumi T, Murtaugh MP, Narayan J, Nguyen DT, Ni P, Oh S-J, Onteru S, Panitz F, Park E-W, Park H-S, Pascal G, Paudel Y, Perez-Enciso M, Ramirez-Gonzalez R, Reecy JM, Rodriguez-Zas S, Rohrer GA, Rund L, Sang Y, Schachtschneider K, Schraiber JG, Schwartz J, Scobie L, Scott C, Searle S, Servin B, Southey BR, Sperber G, Stadler P, Sweedler JV, Tafer H, Thomsen B, Wali R, Wang J, Wang J, White S, Xu X, Yerle M, Zhang G, Zhang J, Zhang J, Zhao S, Rogers J, Churcher C, Schook LB (2012). Analyses of pig genomes provide insight into porcine demography and evolution. NATURE, 491(7424), 393 - 398. doi:10.1038/nature11622
    • Crossan C, Baker PJ, Craft J, Takeuchi Y, Dalton HR, Scobie L (2012). Hepatitis E Virus Genotype 3 in Shellfish, United Kingdom. EMERGING INFECTIOUS DISEASES, 18(12), 2085 - 2087. doi:10.3201/eid1812.120924
    • Knight S, Zhang F, Mueller-Kuller U, Bokhoven M, Gupta A, Broughton T, Sha S, Antoniou MN, Brendel C, Grez M, Thrasher AJ, Collins M, Takeuchi Y (2012). Safer, silencing-resistant lentiviral vectors: optimization of the ubiquitous chromatin-opening element through elimination of aberrant splicing.. J Virol, 86(17), 9088 - 9095. doi:10.1128/JVI.00485-12
    • Montiel-Equihua CA, Zhang L, Knight S, Saadeh H, Scholz S, Carmo M, Alonso-Ferrero ME, Blundell MP, Monkeviciute A, Schulz R, Collins M, Takeuchi Y, Schmidt M, Fairbanks L, Antoniou M, Thrasher AJ, Gaspar HB (2012). The β-globin locus control region in combination with the EF1α short promoter allows enhanced lentiviral vector-mediated erythroid gene expression with conserved multilineage activity.. Mol Ther, 20(7), 1400 - 1409. doi:10.1038/mt.2012.50
    • Fishman JA, Scobie L, Takeuchi Y (2012). Xenotransplantation-associated infectious risk: a WHO consultation.. Xenotransplantation, 19(2), 72 - 81. doi:10.1111/j.1399-3089.2012.00693.x
    • Mattiuzzo G, Takeuchi Y, Scobie L (2012). Potential zoonotic infection of porcine endogenous retrovirus in xenotransplantation.. Methods Mol Biol, 885, 263 - 279. doi:10.1007/978-1-61779-845-0_17

    2011

    • Winkler S, Takeuchi Y, Young N, Thorpe R, Collins M, Zhao Y (2011). SEC purification of lentiviral vectors: comparison of RD114 pseudotypes produced by stable packaging cells and transiently produced VSV-G pseudotypes. HUM GENE THER, 22(10), A109 - A110.
    • Scobie L, Wali R, Bauser C, Rogel-Galliard C, Blomberg J, Sperber G, Takeuchi Y (2011). PORCINE ENDOGENOUS RETROVIRUS (PERV) INTEGRATION IN THE PIG GENOME. TRANSPL INT, 24, 351 - 352.
    • Busby S, Lang S, Takeuchi Y, Vesely P, Petersen B, Galli C, Scobie L (2011). MRSA: A problem for xenotransplantation?. XENOTRANSPLANTATION, 18(5), 305 - 305.
    • Scobie L, Crossan C, Matouskova M, Hector R, Cozzi E, Tallachini MC, Soulillou JP, Ribes J, Takeuchi Y, Hejnar J, Blaha J, Vesely P (2011). No long term transmission of PERV in fifty five patients receiving porcine skin xenografts. XENOTRANSPLANTATION, 18(5), 280 - 280.
    • Scobie L, Hector R, Matouskova M, Ribes J, Mattiuzzo G, Crossan C, Hejnar J, Takeuchi Y (2011). PERV-C integration, expression and transmission of PERV to human cells. XENOTRANSPLANTATION, 18(5), 279 - 280.
    • Busby S, Sharp C, Simmonds P, Takeuchi Y, Scobie L (2011). Emerging pathogens of concern in xenotransplantation. XENOTRANSPLANTATION, 18(5), 278 - 278.
    • Lopes L, Dewannieux M, Takeuchi Y, Collins MK (2011). A lentiviral vector pseudotype suitable for vaccine development.. J Gene Med, 13(3), 181 - 187. doi:10.1002/jgm.1553
    • Scobie L, Wali R, Bauser C, Rogel-Gaillard C, Blomberg J, Sperber G, Takeuchi Y (2011). Porcine endogenous retrovirus (PERV) Integration in the pig genome. XENOTRANSPLANTATION, 18(5), 280 - 280.
    • Matouskova M, Vesely P, Scobie L, Takeuchi Y, Hejnar J (2011). CpG hypomethylation as a marker of transcriptionally active porcine endogenous retroviruses. XENOTRANSPLANTATION, 18(5), 280 - 280.
    • Mueller NJ, Takeuchi Y, Mattiuzzo G, Scobie L (2011). Microbial safety in xenotransplantation. CURR OPIN ORGAN TRAN, 16(2), 201 - 206. doi:10.1097/MOT.0b013e32834486f6
    • Shimizu A, Baratchian M, Takeuchi Y, Escors D, Macdonald D, Barrett T, Bagneris C, Collins M, Noursadeghi M (2011). Kaposi's sarcoma-associated herpesvirus vFLIP and human T cell lymphotropic virus type 1 Tax oncogenic proteins activate IkappaB kinase subunit gamma by different mechanisms independent of the physiological cytokine-induced pathways.. J Virol, 85(14), 7444 - 7448. doi:10.1128/JVI.02337-10
    • Scobie L, Crossan C, Busby S, Matouskova M, Hejnar J, Blaha J, Vesely P, Petersen B, Takeuchi Y (2011). The hepatitis E virus does not appear to be transmitted via xenotransplantation. XENOTRANSPLANTATION, 18(5), 278 - 279.
    • Scobie L, Crossan C, Matouskova M, Hector R, Cozzi E, Tallachini MC, Soulillou JP, Ribes J, Takeuchi Y, Hejnar J, Blaha J, Vesely P (2011). NO LONG TERM TRANSMISSION OF PERV IN FIFTY FIVE PATIENTS RECEIVING PORCINE SKIN XENOGRAFTS. TRANSPL INT, 24, 213 - 213.

    2010

    • Sakuma T, De Ravin SS, Tonne JM, Thatava T, Ohmine S, Takeuchi Y, Malech HL, Ikeda Y (2010). Characterization of Retroviral and Lentiviral Vectors Pseudotyped with Xenotropic Murine Leukemia Virus-Related Virus Envelope Glycoprotein. HUM GENE THER, 21(12), 1665 - 1673. doi:10.1089/hum.2010.063
    • Takeuchi Y, Fishman J (2010). Long life with or without PERV.. Xenotransplantation, 17(6), 429 - 430. doi:10.1111/j.1399-3089.2010.00614.x
    • Shimizu A, Nakatani Y, Nakamura T, Ishikawa O, Takeuchi Y, Hoshino H (2010). Generation of cytoplasmic DNA of an RNA virus by human LINE-1.
    • Mattiuzzo G, Takeuchi Y (2010). Suboptimal porcine endogenous retrovirus infection in non-human primate cells: implication for preclinical xenotransplantation.. PLoS One, 5(10), e13203 - . doi:10.1371/journal.pone.0013203
    • Knight SB, Bokhoven M, Gubta A, Zhang F, Antoniou M, Thrasher A, Collins M, Takeuchi Y (2010). Insertional Mutagenesis by Lentiviral Vectors.
    • Knight S, Bokhoven M, Collins M, Takeuchi Y (2010). Effect of the Internal Promoter on Insertional Gene Activation by Lentiviral Vectors with an Intact HIV Long Terminal Repeat (vol 84, pg 4856, 2010). J VIROL, 84(12), 6263 - 6263. doi:10.1128/JVI.00774-10
    • Knight S, Bokhoven M, Collins M, Takeuchi Y (2010). Effect of the internal promoter on insertional gene activation by lentiviral vectors with an intact HIV long terminal repeat.. J Virol, 84(9), 4856 - 4859. doi:10.1128/JVI.02476-09
    • Mattiuzzo G, Ivol S, Takeuchi Y (2010). Regulation of porcine endogenous retrovirus release by porcine and human tetherins.. J Virol, 84(5), 2618 - 2622. doi:10.1128/JVI.01928-09

    2009

    • Mattiuzzo G, Takeuchi Y (2009). PERV-A receptors in non-human primates: implication for their employment as an animal model for xenotransplantation.
    • Wood A, Webb BL, Bartosch B, Schaller T, Takeuchi Y, Towers GJ (2009). Porcine endogenous retroviruses PERV A and A/C recombinant are insensitive to a range of divergent mammalian TRIM5alpha proteins including human TRIM5alpha.. Journal of General Virology, 90, 702 - 709.
    • Crossan CL, Busby S, Wang HR, Takeuchi Y, Banks M, Scobie L (2009). Hepatitis E virus: a potential risk for xenotransplantation?.
    • Bokhoven M, Stephen SL, Knight S, Gevers EF, Robinson IC, Takeuchi Y, Collins MK (2009). Insertional gene activation by lentiviral and gammaretroviral vectors.. J Virol, 83(1), 283 - 294. doi:10.1128/JVI.01865-08
    • Gupta RK, Mlcochova P, Pelchen-Matthews A, Petit SJ, Mattiuzzo G, Pillay D, Takeuchi Y, Marsh M, Towers GJ (2009). Simian immunodeficiency virus envelope glycoprotein counteracts tetherin/BST-2/CD317 by intracellular sequestration.. Proc Natl Acad Sci U S A, 106(49), 20889 - 20894. doi:10.1073/pnas.0907075106
    • Scobie L, Takeuchi Y (2009). Porcine endogenous retrovirus and other viruses in xenotransplantation. CURR OPIN ORGAN TRAN, 14(2), 175 - 179. doi:10.1097/MOT.0b013e328327984d
    • Moalic Y, Felix H, Takeuchi Y, Jestin A, Blanchard Y (2009). Genome Areas with High Gene Density and CpG Island Neighborhood Strongly Attract Porcine Endogenous Retrovirus for Integration and Favor the Formation of Hot Spots. J VIROL, 83(4), 1920 - 1929. doi:10.1128/JVI.00856-08
    • Takeuchi Y (2009). Microbial Safety in Xenotransplantation.
    • Hector R, Matouskova M, Hejnar J, Crossan C, Fishman J, Takeuchi Y, Scobie L (2009). PERV-C integration, expression and transmission of perv to human cells: is there a link?.
    • Mattiuzzo G, Ivol S, Takeuchi Y (2009). Tetherin overexpression reduces PERV release from pig cells.
    • Buzdin AA, Matouskova M, Daniel P, Trejbalova K, Vesely P, Takeuchi Y, Hejnar J (2009). Epigenetic and transcriptome-wide approach to the identification of active porcine endogenous Retroviruses.

    2008

    • Mattiuzzo G, Scobie L, Takeuchi Y (2008). Strategies to enhance the safety profile of xenotransplantation: minimizing the risk of viral zoonoses.. Current Opinion in Organ Transplantation, 13(2), 184 - 188.
    • Temperton NJ, Capecchi B, Rappuoli R, de Jong MD, Takeuchi Y, Weiss RA (2008). Measurement of Neutralizing Antibody Responses to Influenza H5N1 Clade 1 A/Viet Nam/1194/04 and Clade 2 A/ Indonesia/5/05 Hemagglutinin Using a Sensitive High Throughput Luciferase- Based Retroviral Pseudotype Assay. In Katz JM (Ed.), Options for the Control of Influenza VI (pp. 94 - 96). : International Medical Press.
    • Knight S, Bokhoven M, Gupta A, Ceylan M, Zhang F, Antoniou M, Thrasher A, Takeuchi Y, Collins M (2008). Insertional mutagenesis by retroviral and lentiviral vectors.
    • Takeuchi Y, Myers R, Danos O (2008). Recombination and Population Mosaic of a Multifunctional Viral Gene, Adeno-Associated Virus cap. PLoS ONE, 3((2): e1634), e1634 - .
    • Takeuchi Y, McClure MO, Pizzato M (2008). Identification of gammaretroviruses constitutively released from cell lines used for human immunodeficiency virus research.. J Virol, 82(24), 12585 - 12588. doi:10.1128/JVI.01726-08

    2007

    • Vart RJ, Nikitenko LL, Leonid L, Lagos D, Trotter MWB, Cannon M, Bourboulia D, Gratrix F, Takeuchi B, C (2007). Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoletin-2 expression in lymphatic endothelial cells. Cancer Research, 67(9), 4042 - 4051.
    • Reuss S, Biese P, Cosset FL, Takeuchi Y, Uckert W (2007). Suspension packaging cell lines for the simplified generation of T-cell receptor encoding retrovirus vector particles. GENE THER, 14(7), 595 - 603. doi:10.1038/sj.gt.3302906
    • Bokhoven M, Stephen S, Takeuchi Y, Collins MK (2007). Insertional mutagenesis by retroviral and lentiviral vectors.
    • Pichlmair A, Diebold SS, Gschmeissner S, Takeuchi Y, Ikeda Y, Collins MK, Reis e Sousa C (2007). Tubulovesicular structures within vesicular stomatitis virus G protein-pseudotyped lentiviral vector preparations carry DNA and stimulate antiviral responses via Toll-like receptor 9. Journal of Virology, 81(2), 539 - 547.
    • Pichlmair A, Diebold SS, Gschmeissner S, Takeuchi Y, Ikeda Y, Collins MK, Reis E Sousa C (2007). Tubulovesicular structures within VSV-G pseudotyped lentiviral vector preparations carry DNA and stimulate anti-viral responses via toll-like receptor 9. Journal of Virology, 81(2), 539 - 547.
    • Mattiuzzo G, Matouskova M, Takeuchi Y (2007). Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species.. Retrovirology, 4, 93 - . doi:10.1186/1742-4690-4-93
    • Temperton NJ, Hoschler K, Major D, Nicolson C, Manvell R, Hien VM, Ha DQ, de Jong MD, Zambon M, Takeuchi Y, Weiss RA (2007). A sensitive retroviral pseudotype assay for Influenza H5N1 neutralizing antibodies. Influenza and other respiratory viruses, 1(3), 105 - 112. doi:10.1111/j.1750-2659.2007.00016.x

    2006

    • Takeuchi Y (2006). PERV evolution: recombination and receptor.
    • Clements MO, Godfrey A, Crossley J, Wilson SJ, Takeuchi Y, Boshoff C (2006). Lentiviral manipulation of gene expression in human adult and embryonic stem cells. TISSUE ENGINEERING, 12(7), 1741 - 1751.

    2005

    • Strang BL, Takeuchi Y, Relander T, Richter J, Bailey R, Sanders DA, Collins MK, Ikeda Y (2005). HIV-1 vectors with alphavirus envelope glycoproteins produced from stable packaging cells. Journal of Virology, 79, 1765 - 1671.
    • Godfrey A, Anderson WJ, Papanastasiou A, Takeuchi Y, Boshoff C (2005). Inhibiting primary effusion lymphoma, by lentiviral vectors encoding short hairpin RNA. Blood, 105(6), 2510 - 2518. doi:10.1182/blood-2004-08-3052
    • Temperton NJ, Chan PK, Simmons G, Zambon MC, Tedder RS, Takeuchi Y, Weiss RA (2005). Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes.. Emerging Infectious Diseases, 11(3), 411 - 416.
    • Relander T, Johansson M, Olsson K, Ikeda Y, Takeuchi Y, Collins MK, Richter J (2005). Gene transfer to repopulating CD34+ cells using amphotropic, GALV or RD114 pseudotyped HIV-1 based vectors from stable producer cells. Molecular Therapy, 11, 425 - 429.
    • Strang BL, Takeuchi Y, Relander T, Richter J, Bailey R, Sanders DA, Collins MK, Ikeda Y (2005). Human immunodeficiency virus type 1 vectors with alphavirus envelope glycoproteins produced from stable packaging cells.. J Virol, 79(3), 1765 - 1771. doi:10.1128/JVI.79.3.1765-1771.2005
    • Relander T, Johansson M, Olsson K, Ikeda Y, Takeuchi Y, Collins M, Richter J (2005). Gene transfer to repopulating human CD34(+) cells using amphotropic-, GALV-, or RD114-pseudotyped HIV-1-based vectors from stable producer cells. MOL THER, 11(3), 452 - 459. doi:10.1016/j.ymthe.2004.10.014
    • Takeuchi Y (2005). PERV genetics: is recombination a threat?.
    • Myers RE, Gale CV, Harrison A, Takeuchi Y, Kellam P (2005). A statistical model for HIV-1 sequence, classification using the subtype analyser (STAR). Bioinformatics, 21, 3535 - 3540.
    • Takeuchi Y, Magre S, Patience C (2005). The potential hazards of xenotransplantation: an overview. Review of Science Technology, 24, 323 - 334.

    2004

    • Richter J, Johansson M, Relander T, Olsson K, Ikeda Y, Takeuchi Y, Collins M (2004). Gene transfer to repopulating human CD34+cells using amphotropic, GALV or RD114 pseudotyped HIV-1 based vectors from stable producer cells..
    • Bartosch B, Stefanidis D, Myers R, Weiss RA, Patience C, Takeuchi Y (2004). Evidence and consequence of porcine endogenous retrovirus recombination. Journal of Virology, 78(24), 13880 - 13890. doi:10.1128/JVI.78.24.13880-13890.2004
    • Strang BL, Ikeda Y, Cosset FL, Collins MK, Takeuchi Y (2004). Characterization of HIV-1 vectors with gammaretrovirus envelope glycoproteins produced from stable packaging cells. Gene Therapy, 11(7), 591 - 598.
    • Harrison I, Takeuchi Y, Bartosch B, Stoye JP (2004). Determinants of high titer in recombinant porcine endogenous retroviruses. Journal of Virology, 78(24), 13871 - 13879.
    • Shimojima M, Miyazawa T, Ikeda Y, McMonagle EL, Haining H, Akashi H, Takeuchi Y, Hosie MJ, Willett BJ (2004). Use of CD134 as a primary receptor by the feline immunodeficiency virus. Science, 303(S661), 1192 - 1195. doi:10.1126/science.1092124
    • Godfrey A, Anderson J, Papanastasiou A, Takeuchi Y, Boshoff C (2004). Related ArInhibiting primary effusion lymphoma by lentiviral vectors encoding short hairpin RNA.. Blood, , - .
    • Magre S, Takeuchi Y, Langford G, Richards A, Patience C, Weiss RA (2004). Reduced sensitivity to human serum inactivation of enveloped viruses produced by pig cells transgenic for human CD55 or deficient for the galactosyl-alpha(1-3) galactosyl epitope. Journal of Virology, 78(11), 5812 - 5819.

    2003

    • Besnier C, Ylinen L, Strange B, Lister A, Takeuchi Y, Goff SP, Towers G (2003). Characterization of murine leukaemia virus restriction in mammals. Journal of Virology, 77, 13403 - 13406.
    • Relander T, Johansson M, Olsson K, Ikeda Y, Takeuchi Y, Collins M, Richter J (2003). Gene transfer to repopulating human CD34+cells using amphotropic or RD114 pseudotyped HIV-1 based vectors from permanent producer cell lines (STAR).
    • Ericksson TA, Takeuchi Y, Templin C, Quinn G, Farhadian SF, Wood JC, Oldmixon BA, Suling KM, Ishii JK, Kitagawa Y, Miyazawa Y, Salomon DR, Weiss RA, Patience C (2003). Identification of receptors for pig endogenous retrovirus. Proceedings of the National Academy of Sciences of the United States of America, 100(11), 6759 - 6764. doi:10.1073/pnas.1138025100
    • Ikeda Y, Takeuchi Y, Martin F, Cosset FL, Mitrophanous K, Collins MK (2003). Continuous high-titre HIV-1 vector production. Nature Biotechnology, 21(5), 569 - 572. doi:10.1038/nbt815
    • Andrawiss M, Takeuchi Y, Hewlett L, Collins MK (2003). Murine leukemia virus particle assembly quantitated by fluorescence microscopy: role of gag-gag interactions and membrane association. Journal of Virology, 77, 11651 - 11660. doi:10.1128/JVI.77.21.11651-11660.2003
    • Magre SM, Takeuchi Y, Bartosch B (2003). Xenotransplantation and pig endogenous retroviruses. Review of Molecular Virology, 13, 311 - 329.
    • Raisch KP, Pizzato M, Sun HY, Takeuchi Y, Cashdollar LW, Grossberg SE (2003). Molecular cloning, complete sequence and biological characterization of a xenotropic murine leukaemia virus constitutively released from the human B-lymphoblastoid cell. VIROLOGY, 308, 83 - 91.

    2002

    • Walker SJ, Pizzato M, Takeuchi Y, Devereux S (2002). Heparin binds to murine leukaemia virus and inhibits Env-independent attachment and infection. Journal of Virology, 76, 6909 - 6918.
    • Towers G, Collins MK, Takeuchi Y (2002). Abrogation of Ref1 restriction in human cells. Journal of Virology, 76(5), 2548 - 2550. doi:10.1128/jvi.76.5.2548-2550.2002
    • Bartosch B, Weiss RA, Takeuchi Y (2002). PCR-based cloning and immuncytological titration of infectious porcine endogenous retrovirus subgroup A and B. Journal of General Virology, 83(pt 9), 2231 - 2240.
    • Ikeda Y, Collins MK, Radcliffe PA, Mitrophanous KA, Takeuchi Y (2002). Gene transduction efficiency in cells of different species by HIV and EIAV vectors. Gene Therapy, 9(14), 932 - 938.
    • Besnier C, Takeuchi Y, Towers GJ (2002). Restriction of lentivirus in monkeys. Proceedings of the National Academy of Sciences of the United States of America, 99(18), 11920 - 11925. doi:10.1073/pnas.172384599

    2001

    • Pizzato M, Merten OW, Blair ED, Takeuchi Y (2001). Development of a suspension packaging cell line for production of high titre, serum-resistant murine leukemia virus vectors.. Gene Ther, 8(10), 737 - 745. doi:10.1038/sj.gt.3301457
    • Devereux S, Walker SJ, Pizzato M, Takeuchi Y (2001). Heparin binds to murine leukaemia virus and inhibits both envelope independent attachment and infection.. BLOOD, 98(11), 209A - 210A.
    • Qari SH, Magre S, Garcia-Lerma JG, Hussain AI, Takeuchi Y, Patience C, Weiss RA, Heneine W (2001). Susceptibility of the porcine endogenous retrovirus to reverse transcriptase and protease inhibitors. Journal of Virology, 75(2), 1048 - 1053.
    • Patience C, Switzer WM, Takeuchi Y, Griffiths DJ, Goward ME, Heneine W, Stoye JP, Weiss RA (2001). Multiple groups of novel retroviral genomes in pigs and related species. Journal of Virology, 75(6), 2771 - 2775. doi:10.1128/jvi.75.6.2771-2775.2001
    • Pizzato M, Blair ED, Fling M, Kopf J, Tomassetti A, Weiss RA, Takeuchi Y (2001). Evidence for nonspecific adsorption of targeted retrovirus vector particles to cells. Gene Therapy, 8(14), 1088 - 1096.
    • Patience C, Takeuchi Y, Cosset FL, Weiss RA (2001). MuLV packaging systems as models for estimating/measuring retrovirus recombination frequency. Developmental Biology, 106, 169 - 179.
    • O'Hare M, Bond J, Clarke C, Takeuchi Y, Atherton AJ, Berry C, Moody J, Silver ARJ, Davies DC, Alsop AE, Neville AM, Jat PS (2001). Conditional immortalization of freshly isolated human mammary fibroblasts and endothelial cells.. Proceedings of the National Academy of Sciences of the United States of America, 98(2), 646 - 651. doi:10.1073/pnas.98.2.646

    2000

    • Liu HY, Soda Y, Shimizu N, Haraguchi Y, Jinno A, Takeuchi Y, Hoshino H (2000). CD4-Dependent and CD4-independent utilization of coreceptors by human immunodeficiency viruses type 2 and simian immunodeficiency viruses. VIROLOGY, 278(1), 276 - 288.
    • Takeuchi Y, Weiss RA (2000). Xenotransplantation: reappraising the risk of retroviral zoonosis. CURR OPIN IMMUNOL, 12(5), 504 - 507.
    • Takeuchi Y (2000). Risk of zoonosis in xenotransplantation.
    • Palu G, Parolin C, Takeuchi Y, Pizzato M (2000). Progress with retroviral gene vectors. REV MED VIROL, 10(3), 185 - 202.
    • Weiss RA, Magre S, Takeuchi Y (2000). Infection hazards of xenotransplantation.. J Infect, 40(1), 21 - 25. doi:10.1053/jinf.1999.0604
    • Towers G, Bock M, Martin S, Takeuchi Y, Stoye JP, Danos O (2000). A conserved mechanism of retrovirus restriction in mammals. Proceedings of the National Academy of Sciences of the United States of America, 97(22), 12295 - 12299.

    1999

    • Weiss RA, Griffiths D, Takeuchi Y, Patience C, Venables PJW (1999). Retroviruses: ancient and modern. ARCH VIROL, , 171 - 177.
    • Takeuchi Y (1999). [Retroviral pseudotypes].. Uirusu, 49(1), 11 - 18.

    1998

    • Takeuchi Y, Patience C, Magre S, Weiss RA, Banerjee PT, Le Tissier P, Stoye JP (1998). Host range and interference studies of three classes of pig endogenous retrovirus. Journal of Virology, 72(12), 9986 - 9991.
    • Porter C, Lukacs K, Box G, Takeuchi Y, Collins MKL (1998). Cationic liposomes enhance the rate of transduction by a recombinant retroviral vector in vitro and in vivo. Journal of Virology, 72, 4832 - 4840.
    • Patience C, Takeuchi Y, Cosset FL, Weiss RA (1998). Packaging of endogenous retroviral sequences in retroviral vectors produced by murine and human packaging cells. Journal of Virology, 72(4), 2671 - 2676.
    • Patience C, Patton GS, Takeuchi Y, Weiss RA, McClure MO, Rydberg L, Breimer ME (1998). No evidence of pig DNA or retroviral infection in patients with short-term extracorporeal connection to pig kidneys. The Lancet, 352(9129), 699 - 701.

    1997

    • Le Tissier P, Stoye JP, Takeuchi Y, Patience C, Weiss RA (1997). Two sets of human-tropic pig retrovirus. Nature, 389(6652), 681 - 682.
    • Boshoff C, Endo Y, Collins PD, Takeuchi Y, Reeves JD, Schweickart VL, Siani MA, Sasaki T, Williams TJ, Gray PW, Moore PS, Chang Y, Weiss RA (1997). Angiogenic and HIV-inhibitory functions of KSHV-encoded chemokines. Science, 278(5336), 290 - 294.
    • Patience C, Takeuchi Y, Weiss RA (1997). Infection of human cells by an endogenous retrovirus of pigs. Nature Medicine, 3(3), 282 - 286.
    • Takeuchi Y, Liong SH, Bieniasz PD, Jager U, Porter CD, Friedman T, McClure MO, Weiss RA (1997). Sensitization of rhabdo-, lenti-, and spumaviruses to human serum by galactosyl(alpha1-3)galactosylation. Journal of Virology, 71(8), 6174 - 6178.

    1996

    • Porter CD, Collins MKJ, Tailor CS, Parkar MH, Cossett F-L, Weiss RA, Takeuchi Y (1996). Comparison of efficiency of infection of human gene therapy target cells via four different retroviral receptors. Human Gene Therapy, 7, 913 - 919.
    • Takeuchi Y, Porter CD, Strahan KM, Preece AF, Gustafsson K, Cosset FL, Weiss RA, Collins MK (1996). Sensitization of cells and retroviruses to human serum by (alpha 1-3) galactosyltransferase. Nature, 379(6560), 85 - 88.

    1995

    • Cosset FL, Takeuchi Y, Battini JL, Weiss RA, Collins MK (1995). High-titer packaging cells producing recombinant retroviruses resistant to human serum. Journal of Virology, 69(12), 7430 - 7436.
    • Cosset FL, Morling FJ, Takeuchi Y, Weiss RA, Collins MK, Russell SJ (1995). Retroviral retargeting by envelopes expressing an N-terminal binding domain. Journal of Virology, 69(10), 6314 - 6322.
    • McKnight A, Weiss RA, Shotton C, Takeuchi Y, Hoshino H, Clapham PR (1995). Change in tropism upon immune escape by human immunodeficiency virus. Journal of Virology, 69, 3167 - 3170.

    1994

    • Takeuchi Y, Cosset FL, Lachmann PJ, Okada H, Weiss RA, Collins MK (1994). Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell. Journal of Virology, 68(12), 8001 - 8007.

    1993

    • Tailor CS, Takeuchi Y, O'Hara B, Johann SV, Weiss RA, Collins MK (1993). Mutation of amino acids within the gibbon ape leukemia virus (GALV) receptor differentially affects feline leukemia virus subgroup B, simian sarcoma-associated virus, and GALV infections. Journal of Virology, 67(11), 6737 - 6741.

    1992

    • Takeuchi Y, Vile RG, Simpson G, O'Hara B, Collins MK, Weiss RA (1992). Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus. Journal of Virology, 66(2), 1219 - 1222.