Prof John Ward

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Personal Profile

Name: John Ward Email: j.ward@ucl.ac.uk
Title: Prof Tel: 020 7679 7969
Department: Dept of Biochemical Engineering Fax:
Position: Professor of Synthetic Biology for Bioprocessing Address: The Advanced Centre for Biochemical Engineeering, Department of Biochemical Engineering, Torrington Place, London, WC1E 7JE
Research Domain: Basic Life Sciences, Bioprocessing, Nanotechnology (Frontier Disciplines), Origins, Personalised Medicine, Synthetic Biology Web Page: Personal Web Page

Profile

Research Description

John Ward’s research interests are in the use of bacteria their enzymes and phages to develop new chemicals, pathways and active entities for applications in synthetic biology, biocatalysis and host cell engineering for bioprocessing.John has developed the molecular biology and biochemistry to access many enzymes for biocatalysis for the Bioconversion-Chemistry-Engineering (BiCE) programme, these enzymes include dioxygenases, transketolase, Baeyer-Villiger mono-oxygenases, cytochrome P450's, transaminases and synthetic plant enzymes such as norcoclaurine synthase. The aim, within the BiCE programme is to produce robust biocatalysts and to have many examples of each enzyme type available in our ‘toolbox’ to enable the rapid development of new biocatalytic steps and pathways. The programme brings together a group of researchers from UCL with a long history of collaborative research including Prof Helen Hailes (Chemistry, UCL) together with Prof Paul Dalby, Prof Gary Lye, Dr Frank Baganz, Dr Martina Micheletti, Dr. Darren Nesbeth and Dr Nicolas Szita.  It is supported by a group of 13 leading national and international companies who comprise the BiCE Industrial Steering Group.  In addition, with Prof Helen Hailes in the Department of Chemistry, John is developing the de novo construction of biosynthetic pathways for chiral molecules such as plant alkaloids and recently has discovered antimicrobial activities in novel compounds from this alkaloid project. John is using metagenomics which is a non culturing method of accessing novel enzymes and pathways from the microbiomes of various environments such as the human tongue with Prof Brian Henderson. John collaborates with Prof Christine Orengo (SMB, UCL) who provides the bioinformatics support for the metagenomics projects.The hunt for novel enzymes extends to collaborations in Astrobiology with Dr Ian Crawford and Prof Peter Muller, and the UCL Origin of Life Reactor a Leverhulme Trust funded project with Dr Nick Lane and Dr Julian Evans.   The future inputs for the chemical and pharmaceutical industry need to move from non-renewable petrochemicals to sustainable materials such as plant biomass. The whole bioprocess from the physical, enzymic and chemical treatment to separate the biopolymers such as cellulose, hemicelluolose, pectin and protein through to the depolymerisation of the key polymers and the use of synthetic biology to create engineered pathways to make high value products is being tackled. John is the lead in Synbion, one of the UK’s Networks in Synthetic Biology


Research Activities

Molecular Microbiology

Synthetic Biology

Education Description



I am degree programme tutor of the MRes in Synthetic Biology which I founded in 2010 and I teach on this course and undergraduate courses in the areas of synthetic biology, microbial molecular biology and biochemistry.

UCL Collaborators

Dr Darren Nesbeth; Dr Vitor Bernardes Pinheiro; Dr Morgana Vianna

External Collaborators

Publications

    2014

    • Chauviac FX, Robertson G, Quay DH, Bagnéris C, Dumas C, Henderson B, Ward J, Keep NH, Cohen-Gonsaud M (2014). The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain.. Acta Crystallogr F Struct Biol Commun, 70(Pt 8), 1022 - 1026. doi:10.1107/S2053230X1401317X
    • Richter N, Simon RC, Kroutil W, Ward JM, Hailes HC (2014). Synthesis of pharmaceutically relevant 17-α-amino steroids using an ω-transaminase.. Chem Commun (Camb), , - . doi:10.1039/c3cc49080g
    • Sayer C, Martinez-Torres RJ, Richter N, Isupov MN, Hailes HC, Littlechild JA, Ward JM (2014). The substrate specificity, enantioselectivity and structure of the (R)-selective amine : pyruvate transaminase from Nectria haematococca. FEBS JOURNAL, 281(9), 2240 - 2253. doi:10.1111/febs.12778
    • Sehl T, Hailes HC, Ward JM, Menyes U, Pohl M, Rother D (2014). Efficient 2-step biocatalytic strategies for the synthesis of all nor(pseudo) ephedrine isomers. GREEN CHEMISTRY, 16(6), 3341 - 3348. doi:10.1039/c4gc00100a
    • Grant C, Deszcz D, Wei YC, Martínez-Torres RJ, Morris P, Folliard T, Sreenivasan R, Ward J, Dalby P, Woodley JM, Baganz F (2014). Identification and use of an alkane transporter plug-in for applications in biocatalysis and whole-cell biosensing of alkanes.. Sci Rep, 4, 5844 - . doi:10.1038/srep05844

    2013

    • Sehl T, Hailes HC, Ward JM, Wardenga R, von Lieres E, Offermann H, Westphal R, Pohl M, Rother D (2013). Two Steps in One Pot: Enzyme Cascade for the Synthesis of Nor(pseudo)ephedrine from Inexpensive Starting Materials. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 52(26), 6772 - 6775. doi:10.1002/anie.201300718
    • Halim M, Rios-Solis L, Micheletti M, Ward JM, Lye GJ (2013). Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines.. Bioprocess Biosyst Eng, , - . doi:10.1007/s00449-013-1065-5
    • Branston SD, Ward JM, Keshavarz-Moore E, Stanley EC (2013). Determination of the survival of bacteriophage M13 from chemical and physical challenges to assist in its sustainable bioprocessing. Biotechnology and Bioprocess Engineering, 18(3), 560 - 566. doi:10.1007/s12257-012-0776-9
    • Bommer M, Ward JM (2013). A 1-step microplate method for assessing the substrate range of l-α-amino acid aminotransferase.. Enzyme Microb Technol, 52(4-5), 218 - 225. doi:10.1016/j.enzmictec.2013.02.007
    • Schumacher FF, Sanchania VA, Tolner B, Wright ZV, Ryan CP, Smith ME, Ward JM, Caddick S, Kay CW, Aeppli G, Chester KA, Baker JR (2013). Homogeneous antibody fragment conjugation by disulfide bridging introduces 'spinostics'.. Sci Rep, 3, 1525 - . doi:10.1038/srep01525
    • Rios-Solis L, Bayir N, Halim M, Du C, Ward JM, Baganz F, Lye GJ (2013). Non-linear kinetic modelling of reversible bioconversions: Application to the transaminase catalyzed synthesis of chiral amino-alcohols. BIOCHEMICAL ENGINEERING JOURNAL, 73, 38 - 48. doi:10.1016/j.bej.2013.01.010
    • Hailes HC, Rother D, Müller M, Westphal R, Ward JM, Pleiss J, Vogel C, Pohl M (2013). Engineering Stereoselectivity of ThDP-dependent Enzymes.. FEBS J, , - . doi:10.1111/febs.12496
    • Dartnell LR, Roberts TA, Moore G, Ward JM, Muller JP (2013). Fluorescence characterization of clinically-important bacteria.. PLoS One, 8(9), e75270 - . doi:10.1371/journal.pone.0075270

    2012

    • Branston S, Stanley E, Keshavarz-Moore E, Ward J (2012). Precipitation of filamentous bacteriophages for their selective recovery in primary purification.. Biotechnol Prog, 28(1), 129 - 136. doi:10.1002/btpr.705
    • Baboo JZ, Galman JL, Lye GJ, Ward JM, Hailes HC, Micheletti M (2012). An automated microscale platform for evaluation and optimization of oxidative bioconversion processes.. Biotechnol Prog, 28(2), 392 - 405. doi:10.1002/btpr.1500
    • Pacheco-Gomez R, Kraemer J, Stokoe S, England HJ, Penn CW, Stanley E, Rodger A, Ward J, Hicks MR, Dafforn TR (2012). Detection of Pathogenic Bacteria Using a Homogeneous Immunoassay Based on Shear Alignment of Virus Particles and Linear Dichroism. ANALYTICAL CHEMISTRY, 84(1), 91 - 97. doi:10.1021/ac201544h
    • Dartnell LR, Patel MR, Storrie-Lombardi MC, Ward JM, Muller J-P (2012). Experimental determination of photostability and fluorescence-based detection of PAHs on the Martian surface. METEORITICS & PLANETARY SCIENCE, 47(5), 806 - 819. doi:10.1111/j.1945-5100.2012.01351.x
    • Lan T, Gerontas S, Smith GR, Langdon J, Ward JM, Titchener-Hooker NJ (2012). Investigating the use of column inserts to achieve better chromatographic bed support.. Biotechnol Prog, 28(5), 1285 - 1291. doi:10.1002/btpr.1597
    • Sayer C, Bommer M, Isupov M, Ward J, Littlechild J (2012). Crystal structure and substrate specificity of the thermophilic serine: pyruvate aminotransferase from Sulfolobus solfataricus. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 68, 763 - 772. doi:10.1107/S0907444912011274
    • Nesbeth DN, Perez-Pardo MA, Ali S, Ward J, Keshavarz-Moore E (2012). Growth and productivity impacts of periplasmic nuclease expression in an Escherichia coli Fab' fragment production strain.. Biotechnol Bioeng, 109(2), 517 - 527. doi:10.1002/bit.23316
    • Strafford J, Payongsri P, Hibbert EG, Morris P, Batth SS, Steadman D, Smith ME, Ward JM, Hailes HC, Dalby PA (2012). Directed evolution to re-adapt a co-evolved network within an enzyme.. J Biotechnol, 157(1), 237 - 245. doi:10.1016/j.jbiotec.2011.11.017
    • Dartnell LR, Page K, Jorge-Villar SE, Wright G, Munshi T, Scowen IJ, Ward JM, Edwards HG (2012). Destruction of Raman biosignatures by ionising radiation and the implications for life detection on Mars.. Anal Bioanal Chem, 403(1), 131 - 144. doi:10.1007/s00216-012-5829-6
    • Sehl T, Simon RC, Hailes HC, Ward JM, Schell U, Pohl M, Rother D (2012). TTC-based screening assay for ω-transaminases: a rapid method to detect reduction of 2-hydroxy ketones.. J Biotechnol, 159(3), 188 - 194. doi:10.1016/j.jbiotec.2011.12.023
    • Virk B, Correia G, Dixon DP, Feyst I, Jia J, Oberleitner N, Briggs Z, Hodge E, Edwards R, Ward J, Gems D, Weinkove D (2012). Excessive folate synthesis limits lifespan in the C. elegans: E. coli aging model. BMC BIOLOGY, 10, - . doi:10.1186/1741-7007-10-67
    • Pesnot T, Gershater MC, Ward JM, Hailes HC (2012). The Catalytic Potential of Coptis japonica NCS2 Revealed - Development and Utilisation of a Fluorescamine-Based Assay. ADVANCED SYNTHESIS & CATALYSIS, 354(16), 2997 - 3008. doi:10.1002/adsc.201200641

    2011

    • Parker BM, Taylor IN, Woodley JM, Ward JM, Dalby PA (2011). Directed evolution of a thermostable l-aminoacylase biocatalyst.. J Biotechnol, 155(4), 396 - 405. doi:10.1016/j.jbiotec.2011.07.029
    • Rios-Solis L, Halim M, Cazares A, Morris P, Ward JM, Hailes HC, Dalby PA, Baganz F, Lye GJ (2011). A toolbox approach for the rapid evaluation of multi-step enzymatic syntheses comprising a 'mix and match' E. coli expression system with microscale experimentation. BIOCATAL BIOTRANSFOR, 29(5), 192 - 203. doi:10.3109/10242422.2011.609589
    • Dartnell LR, Storrie-Lombardi MC, Mullineaux CW, Ruban AV, Wright G, Griffiths AD, Muller JP, Ward JM (2011). Degradation of cyanobacterial biosignatures by ionizing radiation.. Astrobiology, 11(10), 997 - 1016. doi:10.1089/ast.2011.0663
    • Pesnot T, Gershater MC, Ward JM, Hailes HC (2011). Phosphate mediated biomimetic synthesis of tetrahydroisoquinoline alkaloids.. Chem Commun (Camb), 47(11), 3242 - 3244. doi:10.1039/c0cc05282e
    • Mukhopadhyay TK, Allison N, Charlton S, Ward J, Lye GJ (2011). Use of microwells to investigate the effect of quorum sensing on growth and antigen production in Bacillus anthracis Sterne 34F2.. J Appl Microbiol, 111(5), 1224 - 1234. doi:10.1111/j.1365-2672.2011.05143.x
    • Warburton PJ, Allan E, Hunter S, Ward J, Booth V, Wade WG, Mullany P (2011). Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA).. FEMS Microbiol Ecol, 78(2), 349 - 354. doi:10.1111/j.1574-6941.2011.01166.x
    • Hunter SJ, Easton S, Booth V, Henderson B, Wade WG, Ward JM (2011). Selective removal of human DNA from metagenomic DNA samples extracted from dental plaque.. J Basic Microbiol, 51(4), 442 - 446. doi:10.1002/jobm.201000372
    • Branston S, Stanley E, Ward J, Keshavarz-Moore E (2011). Study of robustness of filamentous bacteriophages for industrial applications.. Biotechnol Bioeng, 108(6), 1468 - 1472. doi:10.1002/bit.23066

    2010

    • HAILES HC, DALBY PA, LYE GJ, BAGANZ F, MICHELETTI M, SZITA N, WARD JM (2010). “α,α’-Dihydroxy Ketones and 2-Amino-1,3-diols: Synthetic and Process Strategies using Biocatalysts. Current Organic Chemistry, 14, 1883 - 1893.
    • SMITH MEB, CHEN BH, HIBBERT EG, KAULMANN U, SMITHIES K, GALMAN JL, BAGANZ F, DALBY PA, HAILES HC, LYE GJ, WARD JM, WOODLEY JM, MICHELETTI M (2010). A multi-disciplinary approach toward the rapid and preparative scale biocatalytic synthesis of chiral amino alcohols: a concise transketolase/ω-transaminase-mediated synthesis of (2S,3S)-2-aminopentane-1,3-diol. ORGANIC PROCESS RESEARCH AND DEVELOPMENT, 14, 99 - 107. doi:10.1021/op900190y
    • Dartnell L, HUNTER S, Lovell KV, Coates AJ, Ward JM (2010). Low-temperature ionising radiation resistance of Deinococcus radiodurans and Antarctic Dry Valley bacteria. Astrobiology, 10(7), 717 - 732.
    • Cousins CR, Griffiths AD, Crawford IA, Prosser BJ, Storrie-Lombardi MC, Davis LE, Gunn M, Coates AJ, Jones AP, Ward JM (2010). Astrobiological considerations for the selection of the geological filters on the ExoMars PanCam instrument.. Astrobiology, 10(9), 933 - 951. doi:10.1089/ast.2010.0517
    • Galman JL, Steadman D, Bacon S, Morris P, Smith ME, Ward JM, Dalby PA, Hailes HC (2010). α,α'-Dihydroxyketone formation using aromatic and heteroaromatic aldehydes with evolved transketolase enzymes.. Chem Commun (Camb), 46(40), 7608 - 7610. doi:10.1039/c0cc02911d
    • Mukhopadhyay TK, Allison N, Charlton S, Hudson MJ, Hallis B, King A, Baker R, Noonan S, McGlashan J, West K, Levy MS, Ward JM, Lye GJ (2010). Evaluation of anthrax vaccine production by Bacillus anthracis Sterne 34F(2) in stirred suspension culture using a miniature bioreactor: A useful scale-down tool for studies on fermentations at high containment. BIOCHEM ENG J, 50(3), 139 - 144. doi:10.1016/j.bej.2010.03.011
    • Hailes HC, Dalby PA, Lye GJ, Baganz F, Micheletti M, Szita N, Ward JM (2010). α α'-Dihydroxy Ketones and 2-Amino-1,3-diols: Synthetic and Process Strategies using Biocatalysts.. Current Organic Chemistry, , 1883 - 1893.
    • Smith MEB, Chen BH, Hibbert EG, Kaulmann U, Smithies K, Galman JL, Baganz F, Dalby PA, Hailes HC, Lye GJ, Ward JM, Woodley JM, Micheletti M (2010). A Multi-Disciplinary Approach Toward the Rapid and Preparative Scale Biocatalytic Synthesis of Chiral Amino Alcohols: A Concise Transketolase /ω -Transaminase-Mediated Synthesis of (2S,3S)-2-Aminopentane-1,3-diol. Organic Process Research & Development, 14, 99 - 107.
    • Ward J, Wohlgemuth R (2010). High-Yield Biocatalytic Amination Reactions in Organic Synthesis. CURR ORG CHEM, 14(17), 1914 - 1927.
    • Dartnell LR, Storrie-Lombardi MC, Ward JM (2010). Complete fluorescent fingerprints of extremophilic and photosynthetic microbes. doi:10.1017/S1473550410000224
    • Dartnell LR, Fallaize DR, Whiting SJ, Ward JM (2010). Desiccation resistance of Antarctic Dry Valley bacteria isolated from contrasting locations. ANTARCT SCI, 22(2), 171 - 172. doi:10.1017/S0954102009990745

    2009

    • Hailes HC, Dalby P A L, G J W, J M (2009). Biocatalytic approaches to ketodiols and aminodiols.. Chemistry Today, 27(4), 28 - 31.
    • Smithies K, Smith MEB, Kaulmann U, Galman JL, Ward JM, Hailes HC (2009). Stereoselectivity of an ω-transaminase-mediated amination of 1,3-dihydroxy-1-phenylpropane-2-one. Tetrahedron: Asymmetry, 20, 570 - 574. doi:10.1016/j.tetasy.2009.03.012
    • Dalby PA, Baganz , F B, Hailes , H C, Lye , G J, Ward , J M (2009). Protein and pathway engineering in biocatalysis. Chemistry Today, 27(4), 18 - 22.
    • Bryant WA, Krabben P, Baganz F, Zhou Y, Ward JM (2009). The Analysis of Multiple Genome Comparisons in Genus Escherichia and Its Application to the Discovery of Uncharacterised Metabolic Genes in Uropathogenic Escherichia coli CFT073.. Comp Funct Genomics, , 782924 - . doi:10.1155/2009/782924
    • Balasundaram B, Nesbeth D, Ward JM, Keshavarz-Moore E, Bracewell DG (2009). Step change in the efficiency of centrifugation through cell engineering: co-expression of Staphylococcal nuclease to reduce the viscosity of the bioprocess feedstock.. Biotechnol Bioeng, 104(1), 134 - 142. doi:10.1002/bit.22369
    • Cousins CR, Ward JD, Crawford IA, Towner MC, Jones AP (2009). Life in subglacial lavas as an analogue for life on Mars.
    • Arulmuthu ER, Versteeg HK, Williams DJ, Tait AS, Ward J, Hoare M (2009). Aerosol Delivery of Plasmid DNA: A Design-of-Experiments Study.
    • Schell U, Wohlgemuth R, Ward JM (2009). Synthesis of pyridoxamine 5’-phosphate using an MBA:pyruvate transaminase as biocatalyst. Journal of Molecular Catalysis B: Enzymatic, 59(4), 279 - 285. doi:10.1016/j.molcatb.2008.10.005
    • Hobday CE, Crawford IA, Jones AP, Shields GA, Ward JD (2009). Microbial life in alkaline environments on Earth and the potential for life on other planets.

    2008

    • Lye GJ, Baganz F, Chen BH, Dlaby PA, Hailes HC, Hibbert E, Kaulmann U, Micheletti M, Smith MEB, Smithies K, Ward JM, Woodley JM (2008). Accelerating biocatalytic process design: Integrating new tools from biology, chemistry and engineering..
    • Branston SD, Stanley EC, Keshavarz-Moore E, Ward JM (2008). Filamentous Bacteriophage Characterisation for their Large Scale Production.
    • Costelloe SJ, Ward JM, Dalby PA (2008). Evolution of the TPP-dependent enzyme family.. Journal of Molecular Evolution, 66, 36 - 49. doi:10.1007/s00239-007-9056-2
    • Hibbert EG, Senussi T, Smith MEB, Costelloe SJ, Ward JM, Hailes HC, Dalby PA (2008). Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde.. Journal of Biotechnology, 134, 240 - 245. doi:10.1016/j.jbiotec.2008.01.018
    • Stanley EC, Branston SD, Keshavarz-Moore E, Ward JM (2008). Bacteriophages: Nature and Exploitation meeting.
    • Dartnell LR, Desorgher L, Ward JM, Coates AJ (2008). Modelling the surface and subsurface Martian radiation environment: implications for astrobiology. INT J ASTROBIOL, 7(1), 64 - 64.
    • Mullen LM, Bossé JT, Nair SP, Ward JM, Rycroft AN, Robertson G, Langford PR, Henderson B (2008). Pasteurellaceae comE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins. PLoS One, 3(12), 1 - 11. doi:10.1371/journal.pone.0003991
    • Mullen LM, Nair SP, Ward JM, Rycroft AN, Williams RJ, Robertson G, Mordan NJ, Henderson B (2008). Novel adhesin from Pasteurella multiocida that binds to the integrin-binding fibronectin FNIII 9-10 repeats. Infection and Immunity, 76(3), 1093 - 1104.
    • Kong S, Rock CF, Booth A, Willoughby N, O'Kennedy RD, Relton J, Ward JM, Hoare M, Levy MS (2008). Large-scale plasmid DNA processing: evidence that cell harvesting and storage methods affect yield of supercoiled plasmid DNA.. Biotechnology and Applied Biochemistry, , - .
    • Branston SD, Stanley EC, Keshavarz-Moore E, Ward JM (2008). Aspects of large scale filamentous bacteriophage production: particle characterisation and contained fermentation..
    • Branston SD, Stanley EC, Keshavarz-Moore E, Ward JM (2008). Aspects of large scale filamentous bacteriophage production: particle.
    • Branston SD, Stanley EC, Keshavarz-Moore E, Ward JM (2008). Filamentous bacteriophage versus plasmids: aspects of their large scale bioprocessing..
    • Bryant WA, Krabben P, Baganz F, Zhou Y, Ward JM (2008). Stoichiometric models and their application to growth in defined conditions..
    • Bryant WA, Krabben P, Baganz F, Zhou Y, Ward JM (2008). Multiple genome synteny comparisons: application to inferring metabolic models and insight into the behaviour of cells in defined conditions..
    • Yau SY, Keshavarz-Moore E, Ward J (2008). Host strain influences onsupercoiled plasmid DNA production in E.coli; implications for efficient design of large scale processes.. Biotechnology and Bioengineering, 101(3), 529 - 544. doi:10.1002/bit.21915
    • Gill NK, Mukhopadhyay T, Baganz F, Ward JM, Levy MS, Lye GJ (2008). Design and evaluation of a parallel minature bioreactor system for high throughput bioprocess development.
    • Yau SY, Ward J, Keshavarz-Moore E (2008). Host strain influences on supercoiled plasmid DNA production in E.coli; implications for efficient design of large scale processes.
    • Hilker I, Gutiérrez MC, Furstos R, Ward J, Wohlgemuth R AV (2008). Preparative scale Baeyer-Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process.. Nature Protocols, 3, 546 - 554.
    • Yau SY, Ward J, Keshavarz-Moore E (2008). Host Strain Influences on Supercoiled Plasmid DNA Production in E.coli; implications for efficient design of large scale processes..

    2007

    • Dartnell LR, Desorgher R, Ward JM, Coates AJ (2007). Modelling the surface and subsurface martian radiation environment: implications for astrobiology. Geophysical Review Letters, 34, - . doi:10.1029/2006GL027494
    • Baganz F, Chen BH, Dalby PA, Hailes HC, Hibbert E, Kaulmann U, Lye GJ, Micheletti M, Smith MEB, Smithies K, Ward JM, and Woodley JM (2007). Accelerating biocatalytic process design: Integrating new tools from biology, chemistry and engineering. Journal of Biotechnology, 131, S78 - S78.
    • Chen BH, Sayar A, Kaulmann U, Dalby PA, Ward JM, Woodley JM (2007). Reaction modelling and simulation to assess the inergrated use of transketolase and ω-transaminase for the synthesis of an aminotriol.. Biocatalysis and Biotransformations, 24, 449 - 457.
    • Akinrinsola I, Krabben P, Baganz F, Sheridan RAW, J (2007). Physiological characterization of Saccharopolyspora erythraea deletion strains: Global and pathway specific regulators.
    • Kaulmann U, Smithies K, Mark E B S, M E B H, H C W, JM (2007). Substrate spectrum of ?-transaminase from Chromobacterium violaceum DSM30191 and its potential for biocatalysis. Enzyme and Microbial Technology, 41(5), 628 - 637. doi:10.1016/j.enzmictec.2007.05.01
    • Hibbert EG, Senussi T, Costelloe SJ, Lei W, Smith MEB, Ward JM, Hailes HC, Dalby PA (2007). . Directed evolution of transketolase activity on non-phosphorylated substrates.. Journal of Biotechnology, 131(4), 425 - 432. doi:10.1016/j.jbiotec.2007.07.949
    • Meacle FJ, Zhang H, Papantoniou I, Ward JM, Titchener-Hooker NJ, Hoare M (2007). Degradation of supercoiled plasmid DNA within a capillary device. BIOTECHNOL BIOENG, 97(5), 1148 - 1157. doi:10.1002/bit.21275
    • Mullen LM, Nair SP, Ward JM, Rycroft AN, Williams RJ, Henderson B (2007). Comparative functional genomic analysis of Pasteurellaceae adhesins using phage display. doi:10.1016/j.vetmic.2006.12.022
    • Ingram CU, Bommer M, Smith MEB, Dalby PA, Ward JM, Hailes H, Lye GJ (2007). One-pot synthesis of amino diols using a de-novo transketolase and ß-alanine:pyruvate transaminase pathway in Escherichia coli.. Biotechnology and Bioengineering, 96, 559 - 569. doi:10.1002/bit.21125
    • Hibbert EG, Senussi T, Costelloe SJ, Lei W, Smith MEB, Ward JM, Hailes HC, Dalby PA (2007). Directed evolution of transketolase activity on non-phosphorylated substrates. Journal of Biotechnology, (134), 240 - 245. doi:10.1016/j.jbiotec.2008.01.018
    • Hibbert EG, Smith MEB, Smithies K, Kaulmann U, Hailes HC, Ward JM, Dalby PA (2007). Comparison of the natural and directed evolution of transketolase..
    • Yau SY, Kay A, O'Kennedy R, Cooke J, Cooke GD, Ward J, Keshavarz-Moore E (2007). Strategy for preparation of non-viral vectors for therapy and vaccination.
    • Baganz F, Chen BH, Dalby PA, Hailes HC, Hibbert E, Kaulmann U, Lye GJ, Micheletti M, Smith MEB, Smithies K, Ward JM, and Woodley JM (2007). Accelerating biocatalytic process design: Integrating new tools from biology, chemistry and engineering..
    • Akinrinsola I, Krabben P, Baganz F, Sheridan R, Ward J (2007). Physiological characterization of Saccharopolyspora erythraea deletion strains: Global and pathway specific regulators.
    • DARTNELL LR, Ward JM, COATES AJ (2007). Martian sub-surface radiation field: biosignatures and geology. Biogeosciences, 4(iss.), 445 - 492.
    • Islam RS, Mukhopadhyay T, Levy MS, Micheletti M, Ward JMAL, G J (2007). A framework for the rapid optimization and scale-up of protein expression combining microscale experiments and statistical experimental design..
    • Meacle FJ, Zhang H, Papantoniou I, Ward JM, Titchener-Hooker NJ, Hoare M (2007). Degradation of supercoiled plasmid DNA within a capillary device.. Biotechnology and Bioengineering, 97(5), 1148 - 1157.
    • Branston SD, Stanley EC, Keshavarz-Moore E, Ward JM (2007). The large sale fermentation and bioprocessing of genetically engineered filamentous phage to underpin new therapeutic and industrial applications.
    • Branston SD, Stanley EC, Keshavarz-Moore E, Ward JM (2007). The Effect of shear forces on Filamentous Bacteriophage and the implications for their large-scale production, ,.
    • Stanley EC, Branston SD, Keshavarz-Moore E, Ward J (2007). The large-scale fermentation and bioprocessing of genetically eningeered bacteriophage for pharmaceutical applications.
    • Kaulmann U, Smithies K, Smith MEB, HaileS HC, Ward JM (2007). Substrate spectrum of omega-transaminase from Chromobacterium violaceum DSM30191 and its potential for biocatalysis. ENZYME MICROB TECH, 41(5), 628 - 637. doi:10.1016/j.enzmictec.2007.05.011
    • Stanley EC, Branston SD, Keshavarz-Moore E, Ward J (2007). The large-scale fermentation and bioprocessing of genetically engineered bacteriophage.
    • Yau SY, Ward J, Keshavarz-Moore E (2007). Factors Affecting the Efficient Design of an E.coli System for Plasmid DNA Production..
    • Bryant WA, Krabben P, Baganz F, Zhou Y, Ward JM (2007). The search for uncharacterised metabolic genes in E. coli CFT073 using a multiple genome synteny comparison..
    • DARTNELL LR, Desorgher L, Ward JM, COATES AJ (2007). Martian sub-surface ioinising radiation: biosignatures and geology. Biogeosciences, , - .

    2006

    • Branston SD, Stanley EC, Keshavarz-Moore E, Ward JM (2006). The pharmaceutical processing of genetically engineered bacteriophage..
    • Keep NH, Ward JM, Robertson G, Cohen-Gonsaud M, Henderson B (2006). Bacterial resuscitation factors: revival of viable but non-culturable bacteria. Cellular and Molecular Life Sciences, 63(22), 2555 - 2559.
    • Smithies K, Kaulmann U, Smith MEB, Ward JM, Woodley JM, Hailes HC (2006). Combining organocatalysis and enzyme catalysis: Carbon-carbon bon formation followed by amination.
    • Kaulmann U, Smithies K, Smith MEB, Woodley JM, Ward JM (2006). Towards a two-step enzymatic system for chiral aminodiol formulation.
    • Pheasey N, Joseph J, Love C, Coffin R, Hoare M, M Ward JM, Levy MS (2006). A capillary cytometer method to quantitative viable virus particles based on early detection of viral antigens and cellular events within single cells. Journal of Virological Methods, 137(2), 213 - 218.
    • Smith ME, Kaulmann U, Ward JM, Hailes HC (2006). A colorimetric assay for screening transketolase activity. Bioorganic and Medicinal Chemistry, 14(20), 7062 - 7065. doi:10.1016/j.bmc.2006.06.008
    • Ingram CU, Bommer M, Smith MEB, Dalby PA, Ward JM, Hailes H, Lye GJ (2006). One-pot synthesis of amino diols using a de-novo transketolase and ß-alanine:pyruvate transaminase pathway in Escherichia coli.. Biotechnology and Bioengineering, 96, 559 - 569. doi:10.1002/bit.21125
    • Mullen LM, Nair SP, Ward JM, Rycroft AN, Henderson B (2006). Phage display in the study of infectious diseases. Trends in Microbiology, 14(3), 141 - 147.
    • Hailes HC, Smith MEB, Smithies K, Hibbert EG, Kaulmann U, Senussi TO, Costelloe S, Dalby PA, Ward JM, Woodley (2006). Ketodiol and aminodiol synthesis.
    • Hailes HC, Smith MEB, Smithies K, Dalby PA, Ward JM, Woodley JM (2006). Chemoenzymatic synthesis of amniodiols: Engineering and directed evolution of biocatalytic pathways.
    • Chen BH, Kaulmann U, Dalby PA, Ward JM, Woodley JM (2006). Reactor modelling and simulation to assess operation using transketolase and ω-transaminase for the synthesis of aminodiols.
    • Pheasey N, Joseph JR, Coffin R, Hoare M, Ward JM, Levy MS (2006). Quantitation of infective viral particles by capillary cytometry.
    • Kong S, Zhang H, Hoare M, Ward JM, Titchener-Hooker NJ, Levy MS (2006). Plasmid DNA processing for gene therapy and vaccination: studies on the membrane sterilisation filtration step, Vaccine Technology.
    • Micheletti M, Hibbert EG, Kaulmann U, Ingram CU, Ward JM, Dalby PA, Lye GJ (2006). Microscale process evaluation of evolved and metabolically engineered biocatalysts.
    • Smith MEB, Smithies K, Hibbert EG, Kaulmann U, Senussi T, Costelloe S, Hailes HC, Dalby PA, Ward JM, Woodley JM (2006). Biocatalytic and organocatalytic approaches to ketodiols and aminodiols.
    • Keep NH, Ward JM, Cohen-Gonsaud M, Henderson B (2006). Wake up! Peptidoglycan lysis and bacterial non-growth states. Trends in Microbiology, 14(6), 271 - 276.
    • Chen BH, Sayar A, Kaulmann U, Dalby PA, Ward JM, Woodley JM (2006). Reaction modelling and simulation to assess the integrated use of transketolase and ω-transaminase for the synthesis of an aminotriol.. Biocatalysis and Biotransformations, 24, 449 - 457.
    • Chen BH, Sayar A, Kaulmann U, Dalby PA, Ward JM, Woodley JM (2006). 'Reaction modelling and simulation to assess the integrated use of transketolase and w-transaminase for the synthesis of an aminotriol. Biocatalysis and Biotransformations, 24, 449 - 457.
    • Meacle FJ, Zhang H, Papantoniou I, Ward JM, Titchener-Hooker NJ, Hoare M (2006). Degradation of supercoiled plasmid DNA within a capillary device.. Biotechnology and Bioengineering, 97(5), 1148 - 1157. doi:10.1002/bit.21275
    • Oliveira P, Batagov A, Ward J, Baganz F, Krabben P (2006). Identification of Erythrobactin, a Hydroxamate-Type Siderophore Produced by Saccharopolyspora erythraea. Letters in Applied Microbiology, 42, 375 - 380.

    2005

    • Ingram CU, Smith MEB, Bommer M, Dalby PA, Ward JM, Lye GJ (2005). Synthesis of amino alcohols using a de novo engineered transketolase and ß-alanine: pyruvate transiminase pathway in E. coli.
    • Oliviera PH, Batagov A, Ward JM, Baganz F, Krabben P (2005). Identification of Ethrobactin, a Hydroxamate-Type Siderophore produced by Saccharopolyspora erythraea. Letters in Applied Microbiology, , - .
    • Hailes HC, Smith MEB, Smithies K, Dalby PA, Ward JM, Woodley JM (2005). Chemoenzymatic synthesis of amniodiols: Engineering and directed evolution of biocatalytic pathways.
    • Doig SD, Ortiz-Ochoa K, Ward JM, Baganz F (2005). Characterisation of oxygen transfer in miniature and lab-scale bubble column bioreactors and comparison of microbial growth performance based on constant kLa.. Biotechnology Progress, 21, 1175 - 1182. doi:10.1021/bp050064j
    • OrtizOchoa K, Doig SD, Ward JM, Baganz F (2005). A novel method for the measurement of oxygen mass transfer rates in small-scale vessels.. Biochemical Engineering Journal, 25, 63 - 68.
    • Poolman M, Krabben P, Baganz F, Fell D, Ward JM (2005). Genome scale modelling of Streptomyces metabolic networks.
    • Krabben P, Jacques C, Akinrinsola IA, Baganz F, Ward J (2005). Saccharopolyspora erythraea: Connecting genome to process. Metabolic engineering, genome analysis and product flux.
    • Costelloe SJ, Senussi T, Hibbert EG, Smith MEB, Hailes HC, Ward JM, Dalby PA (2005). Evolution and engineering of transketolase substrate specificity.
    • Smith MEB, Ingram CU, Faulkner S, Hibbert E, Micheletti M, Kaulmann K, Hailes H, Lye GJ, Dalby PA, Ward JM, Woodley JM, Baganz F (2005). Engineering and directed evolution of multi-step biocatalytic pathways in bacteria..
    • Hoare M, Levy SM, Bracewell DG, Doig SD, Kong S, Titchener-Hooker N, Ward JM, Dunnill P (2005). Bioprocess engineering issues that would be faced in producing a DNA vaccine at up to 100m (3) fermentation scale for an influenza pandemic. Biotechnology Progress, 21(6), 1577 - 1592. doi:10.1021/bp050190n
    • Micheletti M, Hibbert E, Kaulmann U, Dalby PA, Ward JM, Lye GJ, Woodley JM (2005). Automated microscale process evaluation of recombinant biocatalyst libraries.
    • Micheletti M, Gill N, Chen BH, Dalby PA, Baganz F, Hailes HC, Ward JM, Lye GJ, Woodley JM (2005). Faster development and evaluation of biocatalytic processes.
    • Pheasey N, John J, Love C, Coffin R, Hoare M, M Ward JM, Levy S (2005). A capillary cytometer method to quantitate viable virus particles based on early detection of viral antigens and cellular events within single cells.
    • Ingram CU, Lye GJ, Dalby PA, Ward JM, Bommer M, Hailes HC, Smith MEB (2005). Multistep biocatalytic pathways in bacteria for the synthesis of amniodiols.
    • Krabben P, Oliveira P, Baganz F, Ward J (2005). Exploring the substrate spectrum of the antibiotic producing bacteria Saccharopolyspora erythraea.
    • Cohen-Gonsaud M, Barthe P, Bagneris C, Henderson B, Ward J, Roumestand C, Keep NH (2005). The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozomes. Nature Structural and Molecular Biology, 12(3), 270 - 273. doi:10.1038/nsmb905
    • Hibbert EG, Baganz F, Hailes HC, Ward JM, Lye GJ, Woodley JM, Dalby PA (2005). Directed evolution of biocatalytic processes. Biomolecular Engineering, 22(1-3), 11 - 19.

    2004

    • Cooke JR, McKie EA, Ward JM, Keshavarz-Moore E (2004). Impact of intrinsic DNA structure on processing of plasmids for gene therapy and DNA vaccines. Journal of Biotechnology, 114(3), 239 - 254. doi:10.1016/j.jbiotec.2004.06.011
    • Cooke JR, McKie EA, Ward JM, Keshavarz-Moore E (2004). Impact of intrinsic DNA structure on processing of plasmids for gene therapy and DNA vaccines. Journal of Biotechnology, 114(3), 239 - 254. doi:10.1016/j.jbiotec.2004.06.011
    • Cohen-Gonsaud M, Keep NH, Davies AP, Ward J, Henderson B, Labesse G (2004). Resuscitation promoting factors possess a lysozyme-like domain.. Trends in Biochemical Sciences, 29(1), 7 - 10. doi:10.1016/j.tibs.2003.10.009
    • Cooke JR, McKie EA, Ward JM, Keshavarz-Moore E (2004). Impact of intrinsic DNA structure on processing of plasmids for gene therapy and DNA vaccines. J BIOTECHNOL, 114(3), 239 - 254. doi:10.1016/j.jbiotech.2004.06.011
    • Krabben P, Jaques C, Akinrinsola IA, Ushio M, Baganz FAW, J (2004). Connecting genome to process. Metabolic engineering, genome analysis and product flux..
    • Baganz F, Jacques C, Ushio M, Zhou Y, Ward JM, Leadley P (2004). Examining the effect of genetic modifications in primary metabolism on polyketide production by saccharopolyspora erythraea.

    2003

    • Rock C, Kohl S, Ward J, Levy MS (2003). Plasmid processing for gene therapy and DNA vaccination: effect of centrifugation and resuspension of bacterial cells. Biotechnology Progress (Nucleic Acids Technology edition), , - .
    • Irvine H, Zhou Y, Baganz F, Ward J (2003). Antisense RNA: A metabolic engineering tool used to study overflow metabolism in Escherichia coli.
    • Jaques C, Baganz F, Krabben P, Ward J, Zhou Y (2003). Investigation of the causes of organic acid production during stationary phase in Saccharopolyspora erythraea using an uncoupler of oxidative phosphorylation.
    • Ingram CU, Dalby PA, Hailes HC, Lye GJ, Ward J (2003). Engineering and directed evolution of multi-step biocatalytic pathways in bacteria.
    • Ward J, Baganz F, Krabben P, Fell D, Poolman M, Leadley P (2003). Connecting Genome to Process: the linkage of Saccharopolyspora erythraea metaolic engineering to product flux..
    • O'Kennedy RD, Ward JM, Keshavarz-Moore E (2003). Effects of fermentation strategy on the characteristics of plasmid DNA production. Biotechnology and Applied Biochemistry, 37, 83 - 90.
    • Lotfian P, Levy MS, Coffin RS, Ward J, Shamlou PA (2003). Shear-induced release of disabled herpes simplex virus from baby hamster kidney cells. Biotechnology of Applied Biochemistry, 38(3), 271 - 281.
    • Loftian P, Levy MS, Coffin RS, Ward JM, Shamlou PA (2003). Shear- induced release of disabled herpes simplex virus from BHK cells. Biotechnology and Applied Biochemistry, 38, 271 - 281.
    • Kay A, O'Kennedy RD, Ward JM, Keshavarz-Moore E (2003). Impact of plasmid size on cellular oxygen demand in Escherichia coli. Biotechnology and Applied Biochemistry, 38, 1 - 7.
    • Ingram CU, Dalby PA, Hailes HC, Lye GJ, Ward J (2003). Engineering and directed evolution of multi-step biocatalytic pathways in bacteria.
    • Henderson B, Nair SP, Ward JM, Wilson M (2003). Molecular pathogenicity of the oral opportunistic pathogen Actinobacillus actinomycetemcomitans. Annual Review of Microbiology, 57, 29 - 55.
    • Hussain H, Ward JM (2003). Ferredoxin reductase enhances heterologously expressed cytochrome CYP105D1 in Escherichia coli and Streptomyces lividans. Enzyme and Microbial Technology, 32, 790 - 800.
    • Hussain H, Ward JM (2003). Enhanced Heterologous expression of two Streptomyces griseolus cytochrome P450s and Streptomyces coelicolor Ferredoxin Reductase as potentially efficient hydroxylation catalysts. Applied and Environmental Microbiology, 69, 373 - 382. doi:10.1128/AEM.69.1.373-382.2003
    • Cooke GD, Cranenburgh RM, Hanak JAJ, Ward JM (2003). A modified Eschericia coli protein production strain expressing staphylococcal nuclease, capable of auto-hydrolysing host nucleic acid. Journal of Biotechnology, 101(3), 229 - 239.
    • Peacock LM, Ward J, Ratledge C, Dickinson M, Ison A (2003). How streptomyces lividans uses oils and sugars as mixed substrates. Enzyme and Microbial Technology, 32(1), 157 - 166.

    2002

    • Jaques C, Baganz F, Ward J, Zhou Y (2002). Flux balance analysis of Saccharopolyspora erythraea incorporating changing biomass composition.
    • Irvine H, Ward JM, Zhou Y, Baganz F (2002). Antisense RNA: A metabolic engineering tool, used to regulate overflow metabolism in Escherichia coli.
    • Jaques C, Baganz F, Ward J, Zhou Y (2002). Flux balance analysis of Saccharopolyspora erythraea.
    • Henderson B, Wilson M, Sharp L, Ward JM (2002). Actinobacillus actinomycetemcomitans. Journal of Medical Microbiology, 51(12), 1013 - 1020.
    • Pierce JJ, Robinson SC, Ward JM, Keshavarz-Moore E, Dunnill P (2002). A comparison of the process issues in expressing the same recombinant enzyme periplasmically in Escherichia coli and extracellularly in Streptomyces lividans. Journal of Biotechnology, 92(3), 205 - 216.
    • Williams RJ, Nair SP, Henderson B, Holland KT, Ward JM (2002). Expression of the S. aureus hysA gene in S. carnosus from a modified E. coli-staphylococcal shuttle vector. Plasmid, 47(3), 241 - 245.
    • Ushio M, Ward JM, Baganz F (2002). Physiological control of novel pigmented antibodies produced by Saccharopolyspora erythraea.
    • Keshavarz-Moore E (2002). Fermentation strategy for high yield supercoiled plasmid DNA synthesis.
    • Peacock LM, Ratledge C, Dickinson M, Ward J, Ison A (2002). How streptomyces lividans uses oils and sugars as mixed. Enzyme and Microbial Technology, 32(1), 157 - 166.
    • Pierce JJ, Robinson SC, Ward JM, Keshavarz-Moore E, Dunnill P (2002). A comparison of the process issues in expressing the same recombinant enzyme periplasmically in E.coli and extracellularly in S.lividans.. Journal of Biotechnology, 92, 205 - 215.

    2001

    • Ward J, Fletcher J, Nair SP, Wilson M, Williams RJ, Poole S, Henderson B (2001). Identification of the exported proteins of the oral opportunistic pathogen Actinobacillus actinomycetemcomitans by using alkaline phosphatase fusions. Infection and Immunity, 69(4), 2748 - 2752. doi:10.1128/IAI.69.4.2748-2752.2001
    • Jaques C, Baganz F, Ward J, Zhou Y (2001). Flux balance analysis of Saccharopolyspora erythraea.
    • Ushio M, Ward J, Baganz F (2001). Physiological control of novel pigmented antibiotic produced by Saccharopolyspora Erythraea. Poster presentation.
    • Fletcher JM, Nair SP, Ward JM, Henderson B, Wilson M (2001). Analysis of the effect of changing environmental conditions on the expression patterns of exported surface-associated proteins of the oral pathogen Actinobacillus actinomycetemcomitans. Microbial Pathogenesis, 30(6), 359 - 368.
    • Cooke GD, Cranenburgh RM, Hanak JAJ, Dunnill P, Thatcher DR, Ward JM (2001). Purification of essentially RNA free plasmid DNA using a modified Escherichia Coli host strain expressing ribonuclease A.. Journal of Biotechnology, 85, 297 - 304. doi:10.1016/S0168-1656(00)00378-3
    • Fletcher JM, Nair SP, Ward JM, Henderson B, Wilson M (2001). Analysis of the effect of changing environmental conditions on the expression patterns of exported surface-associated proteins of the oral pathogen Actinobacillus actinomycetemcomitans. MICROB PATHOGENESIS, 30(6), 359 - 368. doi:10.1006/mpat.2001.0439
    • Ward JM (2001). Protein expression in Escherichia coli. In Brown TA (Ed.), Essential Molecular Biology (pp. 209 - 221). : Oxford University Press.
    • Doig SD, O'Sullivan LM, Patel S, Ward JM, Woodley JM (2001). Large scale production of cyclohexanone monooxygenase from Escherichia coli TOP10 pQR239. Enzyme and Microbial Technology, 28, 265 - 274.

    2000

    • Zambianchi F, Pasta P, Ottolina G, Carrea G, Colonna S, Gaggero N, Ward JM (2000). Effect of substrate concentration on the enantioselectivity of cyclohexanone monooxygenase from Acinetobacter calcoaceticus and its rationalization (vol 11, pg 3653, 2000). TETRAHEDRON-ASYMMETR, 11(23), 4817 - 4817.
    • Levy MS, Collins IJ, Tsai JT, Shamlou PA, Ward JM, Dunnill PA (2000). Removal of contaminant nucleic acids by nitrocellulose filtration during pharmaceutical-grade plasmid DNA processing. Journal of Biotechnology, 76(2), 197 - 205.
    • Ward JM, Hanak J, Thatcher D (2000). Preparation of cellular components that are substantially free of chromosomal DNA’.
    • Zambianchi F, Pasta P, Ottolina G, Carrea G, Colonna S, Gaggero N, Ward JM (2000). Effect of substrate concentration on the enantioselectivity of cyclohexanone monooxygenase from Acinetobacter calcoaceticus and its rationalization. TETRAHEDRON-ASYMMETR, 11(18), 3653 - 3657.
    • Williams RJ, Ward JM, Henderson B, Wilson M, Nair SP (2000). Rapid screening for putative exported proteins from Staphylococcus aureus using alkaline phosphatase as a reporter molecule. Molecular Biotechnology, 15(1), 11 - 20.
    • Williams RJ, Ward JM, Henderson B, Poole S, O 'Hara BP, Wilson M, Nair SP (2000). Identification of a novel gene cluster encoding staphylococcal exotoxin- like proteins: characterization of the prototypic gene and its protein product, SET1. Infection and Immunity, 68(8), 4407 - 4415.

    1999

    • Levy MS, Collins IJ, Yim SS, Ward JM, Titchener-Hooker NJ, Ayazi Shamlou P, Dunnill P (1999). Effect of shear on plasmid DNA in solution. Bioprocess and Biosystems Engineering, 20, 7 - 13.
    • Tsai JT, Keshavarz-Moore NE, Ward JM, Hoare M, Ayazi Shamlou P, Dunnill P (1999). Characterisation of plasmid DNA conjugates as a base for their processing. Bioprocess Engineering, 21, 279 - 286.
    • Crompton M, Virji S, Johnson N, Doyle V, Ward JM (1999). The mitochondrial permeability transition pore. Biochemical Society Symposium, 66, 167 - 179.
    • Johnson N, Khan A, Virji S, Ward JM, Crompton M (1999). Import and processing of heart mitochondrial cyclophilin D. European Journal of Biochemistry, 263(2), 353 - 359.
    • Noble M, Agutter PA, Bradley MI, Ward JM, Titchener-Hooker NJ, Buss AD, Turner MK (1999). Computational fluid dynamics and a quantitative polymerase chain reaction as tools for measuring bioprocess containment. Transactions of the Institute of Chemical Engineers, 77, 13 - 21.
    • Ciccolini LAS, Ayazi Shamlou P, Titchener-Hooker NJ, Ward JM, Dunnill P (1999). Rheological properties of chromosomal and plasmid DNA during alkaline lysis reaction. Bioprocess and Biosystems Engineering, 21, 231 - 237.

    1998

    • Sheridan R, Jackson GA, LR , Ward J, Dunnill P (1998). Rational engineering of the TOL meta-cleavage pathway. Biotechnol Bioeng, 58(2-3), 240 - 249.
    • Crompton M, Virji S, Ward JM (1998). Cyclophilin-D binding proteins. Biochemical Society Transactions, 26, - .
    • Crompton M, Virji S, Ward JM (1998). Cyclophilin-D binds strongly to complexes of the voltage dependent anion channel and the adenine nucleotide translocase to form the permeability transition pore. European Journal of Biochemistry, 258, 729 - 735.
    • Ciccolini LAS, Ayazi Shamlou P, Titchener-Hooker NJ, Ward JM, Dunnill P (1998). Time course of SDS-alkaline lysis of recombinant bacterial cells for plasmid release. Biotechnology and Bioengineering, 60, 768 - 770.
    • Sheridan R, Jackson GA, Regan L, Ward JM, Dunnill P (1998). Rational engineering of the TOL meta-cleavage pathway. Biotechnology and Bioengineering, 58, 240 - 249.
    • Levy MS, Collins IJ, Yim SS, Ward JM, Titchener-Hooker NJ, Ayazi Shamlou P, Dunhill P (1998). Effect of shear on plasmid DNA in solution. Bioprocess and Biosystems Engineering, 20, 7 - 13.
    • Ciccolini LAS, Ayazi Shamlou P, Titchener-Hooker NJ, Ward JM, Dunnill P (1998). Time course of the SDS-Alkaline lysis of recombinant bacterial cells for plasmid release. Biotechnology and Bioengineering, 60, 768 - 770. doi:10.1002/(SICI)1097-0290(19981220)60:6<768::AID-BIT13>3.0.CO;2-D
    • Johnson N, Virji S, Ward JM, Crompton M (1998). Mitochondrial import of cyclophilin-D. Biochemical Society Transactions, 26, - .

    1997

    • Theodossiou I, Collins IJ, Ward JM, Thomas ORT, Dunnill P (1997). The processing of a plasmid-based gene from E.coli. Primary recovery by filtration. Bioprocess Engineering, 16, 175 - 183.
    • Fletcher JM, Henderson B, Nair SP, Ward J, Williams R, Wilson M (1997). Identification of Actinobacillus actinomycetemcomitans exported proteins.
    • Byrne H, Norbury J, Ward J (1997). Travelling combustion waves in porous media. MATHEMATICAL ENGINEERING IN INDUSTRY, 6(1), 39 - 62.

    1996

    • Madden T, Ward JM, Ison AP (1996). Organic acid excretion by Streptomyces lividans TK24 during growth on defined carbon and nitrogen sources.. Microbiology, 142 ( Pt 11), 3181 - 3185.
    • French C, Ward JM (1996). Production and modification of E. coli transketolase for large-scale biocatalysis.. Ann N Y Acad Sci, 799, 11 - 18.
    • Tanveer A, Virji S, Andreeva L, Totty NF, Hsuan JJ, Ward JM, Crompton M (1996). Involvement of cyclophilin D in the activation of a mitochondrial pore by Ca2+ and oxidant stress. European Journal of Biochemistry, 238(1), 166 - 172. doi:10.1111/j.1432-1033.1996.0166q.x
    • Wilkinson D, Ward JM, Woodley JM (1996). Choice of microbial host for the naphthalene dioxygenase bioconversion.. J Ind Microbiol, 16(5), 274 - 279.
    • Sloane RP, Ward JM, O'Brien SM, Thomas OR, Dunnill P (1996). Expression and purification of a recombinant metal-binding T4 lysozyme fusion protein.. J Biotechnol, 49(1-3), 231 - 238.

    1993

    • Bahri SM, Ward JM (1993). Sequence of the Streptomyces thermoviolaceus CUB74 alpha-amylase-encoding gene and its transcription analysis in Streptomyces lividans.. Gene, 127(1), 133 - 137.
    • Tarragona-Fiol A, Eggelte HJ, Harbron S, Sanchez E, Taylorson CJ, Ward JM, Rabin BR (1993). Identification by site-directed mutagenesis of amino acids in the B2 subsite of bovine pancreatic ribonuclease A.. Protein Eng, 6(8), 901 - 906.
    • Zaman S, Richards H, Ward J (1993). Identification of the minimal replicon of the streptomycete plasmid pIJ101.. Plasmid, 29(1), 57 - 62. doi:10.1006/plas.1993.1007
    • Wrigley-Jones CA, Richards H, Thomas CR, Ward J (1993). Stability of plasmid vector plJ303 in Streptomyces lividans TK24 during laboratory-scale fermentations.. Biotechnol Bioeng, 41(1), 148 - 155. doi:10.1002/bit.260410119

    1992

    • Tarragona-Fiol A, Taylorson CJ, Ward JM, Rabin BR (1992). Production of mature bovine pancreatic ribonuclease in Escherichia coli.. Gene, 118(2), 239 - 245.

    1990

    • Smith DJ, Burnham MK, Bull JH, Hodgson JE, Ward JM, Browne P, Brown J, Barton B, Earl AJ, Turner G (1990). Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes.. EMBO J, 9(3), 741 - 747.
    • Bahri SM, Ward JM (1990). Cloning and expression of an alpha-amylase gene from Streptomyces thermoviolaceus CUB74 in Escherichia coli JM107 and S. lividans TK24.. J Gen Microbiol, 136(5), 811 - 818.

    1985

    • Oliver SG, Ward JM (1985). A dictionary of genetic engineering.

    1982

    • Ward JM (1982). Analysis of the Inc P-1 group plasmids R906 and R751 and their relationship to RP1.. Plasmid, 8(3), 244 - 252.
    • Ward JM (1982). Physical and genetic analysis of the Inc-W group plasmids R388, Sa, and R7K.. Plasmid, 7(3), 239 - 250.

    1981

    • Ward JM, Wallace LJ, Bennett PM, Robinson MK, Richmond MH (1981). Transposition immunity.. Cold Spring Harb Symp Quant Biol, 45, 183 - 188.
    • CHOPRA I, SHALES SW, WARD JM, WALLACE LJ (1981). Reduced Expression of Tn10-mediated Tetracycline Resistance in Escherichia coli Containing More Than One Copy of the Transposon. Microbiology, 126(1), 45 - 54. doi:10.1099/00221287-126-1-45

    1978

    • Ward JM, Grinsted J (1978). Mapping of functions in the R-plasmid R388 by examination of deletion mutants generated in vitro. Gene, 3(2), 87 - 95. doi:10.1016/0378-1119(78)90053-7

    • Kong S, Rock CF, Booth A, Willoughby N, O'Kennedy RD, Relton J, Ward JM, Hoare M, Levy MS (). Large-scale plasmid DNA processing: evidence that cell harvesting and storage methods affect yield of supercoiled plasmid DNA.. Bioprocess and Biosystems Engineering, 51(1), 43 - 51. doi:10.1042/BA20070174
    • Mullen LM, Nair SP, Ward JM, Rycroft AN, Williams RJ, Henderson B (). Comparative functional genomic analysis of Pasteurellaceae adhesins using phage display.. Veterinary Microbiology, , - .