Prof Buzz Baum

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Personal Profile

Name: Buzz Baum Email: b.baum@ucl.ac.uk
Title: Prof Tel: 0044 20 7679 3040
Department: MRC/UCL Lab for Molecular Cell Biology Fax:
Position: Professor of Cell Biology Address: , , ,
Research Domain: Basic Life Sciences, Cancer, Personalised Medicine Web Page: Personal Web Page

Profile

Research Description

The shape of an animal cell is an important functional property that is determined through a dynamic interplay between intrinsic information and cues from the extracellular environment. In the Baum lab we are exploring the molecular, cellular and physical processes that give rise to the shape of isolated animal cells and cells in the context of an epithelium, and are interested in the morphological processes that go awry during cancer progression. To do so, we study developing flies, human and Drosophila cell culture systems as well as cancer patient tissue using an array of diverse techniques including classical cell biological, genetic and biochemical techniques alongside the latest high-content RNA interference (RNAi) screening, imaging, computational modeling, laser ablation, biophysical tools and micropatterning methodologies.

This research falls into three general areas, all of which focus on the cytoskeleton, the cell and its environment. First, we study how cytoskeletal organisation and dynamics are regulated to control normal and cancer cell shape, and use RNAi to identify conserved, coreregulators of these processes.

Second, we investigate cell rounding in mitosis at the molecular, cellular and physical level, and how this rounding contributes to spindle assembly and faithful division of the genetic material.

Third, we use the dorsal thorax of the fly as a model system to understand the orchestrated processes by which a developing tissue becomes well-ordered and refined, through changes in cell shape, changes in cell-cell junctional dynamics and through protrusion-mediated signalling.

Overall, our aim is to build up a picture of how the dynamic behaviour of individual cells contributes to tissue form and function in normal development and cancer progression.


Research Activities

Cell and tissue morphogenesis in development

Digital Biology

Physical cell

Systems morphogenesis

Education Description

Graduate teaching:

http://www.ucl.ac.uk/systems-biology/phd
http://www.ucl.ac.uk/complex/

UCL Collaborators

Prof Bernard Buxton; Dr Peter Bentley; Dr Thomas Mrsic-Flogel; Dr Beau Lotto; Prof Adam Sillito; Prof Mark Lythgoe; Prof Steven Dakin

External Collaborators

Publications

    2014

    • Lancaster OM, Baum B (2014). Shaping up to divide: Coordinating actin and microtubule cytoskeletal remodelling during mitosis.. Semin Cell Dev Biol, , - . doi:10.1016/j.semcdb.2014.02.015
    • Picone R, Baum B, McKendry R (2014). Plasma Microcontact Patterning (PμCP): A Technique for the Precise Control of Surface Patterning at Small-Scale.. Methods Cell Biol, 119, 73 - 90. doi:10.1016/B978-0-12-416742-1.00005-6

    2013

    • Harris AR, Bellis J, Khalilgharibi N, Wyatt T, Baum B, Kabla AJ, Charras GT (2013). Generating suspended cell monolayers for mechanobiological studies.. Nat Protoc, 8(12), 2516 - 2530. doi:10.1038/nprot.2013.151
    • Csikasz-Nagy A, Escudero LM, Guillaud M, Sedwards S, Baum B, Cavaliere M (2013). Cooperation and competition in the dynamics of tissue architecture during homeostasis and tumorigenesis. SEMINARS IN CANCER BIOLOGY, 23(4), 293 - 298. doi:10.1016/j.semcancer.2013.05.009
    • Lancaster OM, Le Berre M, Dimitracopoulos A, Bonazzi D, Zlotek-Zlotkiewicz E, Picone R, Duke T, Piel M, Baum B (2013). Mitotic rounding alters cell geometry to ensure efficient bipolar spindle formation.. Dev Cell, 25(3), 270 - 283. doi:10.1016/j.devcel.2013.03.014
    • Araujo A, Baum B, Bentley P (2013). The role of chromosome missegregation in cancer development: a theoretical approach using agent-based modelling.. PLoS One, 8(8), e72206 - . doi:10.1371/journal.pone.0072206

    2012

    • Marinari E, Mehonic A, Curran S, Gale J, Duke T, Baum B (2012). Live-cell delamination counterbalances epithelial growth to limit tissue overcrowding.. Nature, 484(7395), 542 - 545. doi:10.1038/nature10984
    • Harris AR, Peter L, Bellis J, Baum B, Kabla AJ, Charras GT (2012). Characterizing the mechanics of cultured cell monolayers.. Proc Natl Acad Sci U S A, 109(41), 16449 - 16454. doi:10.1073/pnas.1213301109
    • Block J, Breitsprecher D, Kühn S, Winterhoff M, Kage F, Geffers R, Duwe P, Rohn JL, Baum B, Brakebusch C, Geyer M, Stradal TE, Faix J, Rottner K (2012). FMNL2 drives actin-based protrusion and migration downstream of Cdc42.. Curr Biol, 22(11), 1005 - 1012. doi:10.1016/j.cub.2012.03.064
    • Davidson LA, Baum B (2012). Making waves: the rise and fall and rise of quantitative developmental biology. DEVELOPMENT, 139(17), 3065 - 3069. doi:10.1242/dev.080093
    • Conte V, Ulrich F, Baum B, Muñoz J, Veldhuis J, Brodland W, Miodownik M (2012). A biomechanical analysis of ventral furrow formation in the Drosophila melanogaster embryo.. PLoS One, 7(4), e34473 - . doi:10.1371/journal.pone.0034473
    • Kunda P, Rodrigues NT, Moeendarbary E, Liu T, Ivetic A, Charras G, Baum B (2012). PP1-mediated moesin dephosphorylation couples polar relaxation to mitotic exit.. Curr Biol, 22(3), 231 - 236. doi:10.1016/j.cub.2011.12.016
    • Matthews HK, Delabre U, Rohn JL, Kunda P, Guck J, Baum B (2012). Changes in ect2 localization couple actomyosin-dependent cell shape changes to mitotic progression. Developmental Cell, 23(2), 371 - 383. doi:10.1016/j.devcel.2012.06.003
    • Matthews HK, Baum B (2012). The metastatic cancer cell cortex: an adaptation to enhance robust cell division in novel environments?. Bioessays, 34(12), 1017 - 1020. doi:10.1002/bies.201200109

    2011

    • Baum B (2011). Finding gold in yellowing papers.. Nat Rev Mol Cell Biol, 12(4), 205 - . doi:10.1038/nrm3092
    • Cohen M, Baum B, Miodownik M (2011). The importance of structured noise in the generation of self-organizing tissue patterns through contact-mediated cell–cell signalling.. J R Soc Interface, 8(59), 787 - 798.
    • Gautier JJ, Lomakina ME, Bouslama-Oueghlani L, Derivery E, Beilinson H, Faigle W, Loew D, Louvard D, Echard A, Alexandrova AY, Baum B, Gautreau A (2011). Clathrin is required for Scar/Wave-mediated lamellipodium formation. J CELL SCI, 124(20), 3414 - 3427. doi:10.1242/jcs.081083
    • Bai SW, Herrera-Abreu MT, Rohn JL, Racine V, Tajadura V, Suryavanshi N, Bechtel S, Wiemann S, Baum B, Ridley AJ (2011). Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. BMC BIOL, 9, - . doi:10.1186/1741-7007-9-54
    • Rohn JL, Sims D, Liu T, Fedorova M, Schöck F, Dopie J, Vartiainen MK, Kiger AA, Perrimon N, Baum B (2011). Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype.. J Cell Biol, 194(5), 789 - 805. doi:10.1083/jcb.201103168
    • Lancaster OM, Baum B (2011). Might makes right: Using force to align the mitotic spindle.. Nat Cell Biol, 13(7), 736 - 738. doi:10.1038/ncb0711-736
    • Baum B, Georgiou M (2011). Dynamics of adherens junctions in epithelial establishment, maintenance, and remodeling.. J Cell Biol, 192(6), 907 - 917. doi:10.1083/jcb.201009141
    • Cohen M, Baum B, Miodownik M (2011). The importance of structured noise in the generation of self-organizing tissue patterns through contact-mediated cell-cell signalling.. J R Soc Interface, 8(59), 787 - 798. doi:10.1098/rsif.2010.0488

    2010

    • Araujo A, Bentley PJ, Baum B (2010). Modelling the Role of Chromosome Missegregation in Cancer Therapies..
    • Sims D, Bursteinas B, Jain E, Gao QO, Baum B, Zvelebil M (2010). The FLIGHT Drosophila RNAi database 2010 update. FLY, 4(4), 344 - 348. doi:10.4161/fly.4.4.13303
    • Cully M, Genevet A, Warne P, Treins C, Liu T, Bastien J, Baum B, Tapon N, Leevers SJ, Downward J (2010). A Role for p38 Stress-Activated Protein Kinase in Regulation of Cell Growth via TORC1. MOL CELL BIOL, 30(2), 481 - 495. doi:10.1128/MCB.00688-09
    • Rohn JL, Baum B (2010). Actin and cellular architecture at a glance.. J Cell Sci, 123(Pt 2), 155 - 158. doi:10.1242/jcs.049759
    • Liu T, Rohn JL, Picone R, Kunda P, Baum B (2010). Tao-1 is a negative regulator of microtubule plus-end growth.. J Cell Sci, 123(Pt 16), 2708 - 2716. doi:10.1242/jcs.068726
    • Cohen M, Georgiou M, Stevenson NL, Miodownik M, Baum B (2010). Dynamic filopodia transmit intermittent Delta-Notch signaling to drive pattern refinement during lateral inhibition.. Dev Cell, 19(1), 78 - 89. doi:10.1016/j.devcel.2010.06.006
    • Picone R, Ren X, Ivanovitch KD, Clarke JD, McKendry RA, Baum B (2010). A polarised population of dynamic microtubules mediates homeostatic length control in animal cells.. PLoS Biol, 8(11), e1000542 - . doi:10.1371/journal.pbio.1000542
    • Brodland GW, Conte V, Cranston PG, Veldhuis J, Narasimhan S, Hutson MS, Jacinto A, Ulrich F, Baum B, Miodownik M (2010). Video force microscopy reveals the mechanics of ventral furrow invagination in Drosophila. P NATL ACAD SCI USA, 107(51), 22111 - 22116. doi:10.1073/pnas.1006591107
    • Georgiou M, Baum B (2010). Polarity proteins and Rho GTPases cooperate to spatially organise epithelial actin-based protrusions.. J Cell Sci, 123(Pt 7), 1089 - 1098. doi:10.1242/jcs.060772
    • King JS, Veltman DM, Georgiou M, Baum B, Insall RH (2010). SCAR/WAVE is activated at mitosis and drives myosin-independent cytokinesis.. J Cell Sci, 123(Pt 13), 2246 - 2255. doi:10.1242/jcs.063735

    2009

    • Kunda P, Baum B (2009). The actin cytoskeleton in spindle assembly and positioning.. Trends Cell Biol, 19(4), 174 - 179. doi:10.1016/j.tcb.2009.01.006
    • Sims D, Duchek P, Baum B (2009). PDGF/VEGF signaling controls cell size in Drosophila.. Genome Biol, 10(2), R20 - . doi:10.1186/gb-2009-10-2-r20
    • Liu T, Sims D, Baum B (2009). Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.. Genome Biol, 10(3), R26 - . doi:10.1186/gb-2009-10-3-r26
    • Asano Y, Jimenez-Dalmaroni A, Liverpool TB, Marchetti MC, Giomi L, Kiger A, Duke T, Baum B (2009). Pak3 inhibits local actin filament formation to regulate global cell polarity. HFSP J, 3(3), 194 - 203. doi:10.2976/1.3100548
    • Conte V, Munoz JJ, Baum B, Miodownik M (2009). Robust mechanisms of ventral furrow invagination require the combination of cellular shape changes. PHYS BIOL, 6(1), - . doi:10.1088/1478-3975/6/1/016010
    • Aghajanian J, Warrell J, Prince SJ, Li P, Rohn J, Baum B (2009). Patch-based Within-Object Classification.

    2008

    • Georgiou M, Marinari E, Burden J, Baum B (2008). Cdc42, Par6, and aPKC regulate Arp2/3-mediated endocytosis to control local adherens junction stability.. Curr Biol, 18(21), 1631 - 1638. doi:10.1016/j.cub.2008.09.029
    • Dobbelaere J, Josue F, Suijkerbuijk S, Baum B, Tapon N, Raff J (2008). A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila. PLOS BIOL, 6(9), 1975 - 1990. doi:10.1371/journal.pbio.0060224
    • Kunda P, Rohn JL, Baum B (2008). Cell shape: taking the heat. Current Biology, 18(11), R470 - R472. doi:10.1016/j.cub.2008.04.032
    • Settleman J, Baum B (2008). Cell shape and tissue morphogenesis. Seminars in Cell and Developmental Biology, 19(3), 213 - 214. doi:10.1016/j.semcdb.2008.02.002
    • Kunda P, Pelling AE, Liu T, Baum B (2008). Moesin controls cortical rigidity, cell rounding and spindle morphogenesis during mitosis. Current Biology, 18(2), 91 - 101. doi:10.1016/j.cub.2007.12.051
    • Basanta D, Miodownik M, Baum B (2008). The evolution of robust development and homeostasis in artificial organisms. PLoS Computational Biology, 4(3), e1000030 - . doi:10.1371/journal.pcbi.1000030
    • Baum B, Cherbas L (2008). Drosophila cell lines as model systems and as an experimental tool.. Methods Mol Biol, 420, 391 - 424. doi:10.1007/978-1-59745-583-1_25
    • Baum B, Settleman J, Quinlan MP (2008). Transitions between epithelial and mesenchymal states in development and disease. Seminars in Cell and Developmental Biology, 19(3), 294 - 308. doi:10.1016/j.semcdb.2008.02.001

    2007

    • Jovceva E, Larsen MR, Waterfield MD, Baum B, Timms JF (2007). Dynamic cofilin phosphorylation in the control of lamellipodial actin homeostasis. Journal of Cell Science, 120(11), 1888 - 1897. doi:10.1242/jcs.004366

    2006

    • Zihni C, Mitsopoulos C, Tavares IA, Baum B, Ridley AJ, Morris JDH (2006). Prostate-derived sterile 20-like kinase 1-alpha induces apoptosis: JNK- and caspase-dependent nuclear localization is a requirement for membrane blebbing. Journal of Biological Chemistry, 282(9), 6484 - 6493. doi:10.1074/jbc.M608336200
    • Sims D, Bursteinas B, Gao Q, Zvelebil M, Baum B (2006). FLIGHT: database and tools for the integration and cross-correlation of large-scale RNAi phenotypic datasets.. Nucleic Acids Res, 34(Database issue), D479 - D483. doi:10.1093/nar/gkj038
    • Echeverri CJ, Beachy PA, Baum B, Boutros M, Buchholz F, Chanda SK, Downward J, Ellenberg J, Fraser AG, Hacohen N, Hahn WC, Jackson AL, Kiger A, Linsley PS, Lum L, Ma Y, Mathey-Prevot B, Root DE, Sabatini DM, Taipale J, Perrimon N, Bernards R (2006). Minimizing the risk of reporting false positives in large-scale RNAi screens. Nature Methods, 3(10), 777 - 779. doi:10.1038/nmeth1006-777
    • Baum B (2006). Left-right asymmetry: actin-myosin through the looking glass.. Curr Biol, 16(13), R502 - R504. doi:10.1016/j.cub.2006.06.011

    2005

    • Baum B, Kunda P (2005). Actin nucleation: spire - actin nucleator in a class of its own.. Curr Biol, 15(8), R305 - R308. doi:10.1016/j.cub.2005.04.004

    2004

    • Baum B (2004). Tissue morphogenesis: Crowd control.. Current Biology, 14(17), R716 - R718.
    • Rosenblatt J, Cramer LP, Baum B, McGee KM (2004). Myosin II-dependent cortical movement is required for centrosome separation and positioning during mitotic spindle assembly.. Cell, 117(3), 361 - 372. doi:10.1016/S0092-8674(04)00341-1
    • Rosenblatt J, Cramer LP, Baum B, McGee K (2004). Myosin-II dependent cortical movement is required for centrosome separation and positioning during mitotic spindle assembly.
    • Biyasheva A, Svitkina T, Kunda P, Baum B, Borisy G (2004). Cascade pathway of filopodia formation downstream of SCAR. Journal of Cell Science, 117(6), 837 - 848. doi:10.1242/jcs.00921
    • Craig G, Baum B (2004). Rnai in a postmodern post-genomic era.. Oncogene Reviews, Nov, - .
    • Baum B, Kunda P, Kiger A, Perrimon N (2004). A genetic dissection of cell morphogenesis in Drosophila. CELL STRUCT FUNCT, 29, 25 - 25.
    • Baum B (2004). Animal development: crowd control.. Curr Biol, 14(17), R716 - R718. doi:10.1016/j.cub.2004.08.047

    2003

    • Jacinto A, Baum B (2003). Actin in development. Mechanisms of Development, 120(11), 1337 - 1349.
    • Kiger AA, Baum B, Jones S, Jones MR, Coulson A, Echeverri C, Perrimon N (2003). A functional genomic analysis of cell morphology using RNA interference.. Journal of Biology, 2(4), 27 - . doi:10.1186/1475-4924-2-27
    • Kunda P, Craig G, Dominguez V, Baum B (2003). Abi, Sra1, and Kette control the stability and localization of SCAR/WAVE to regulate the formation of actin-based protrusions.. Current Biology, 13(21), 1867 - 1875. doi:10.1016/j.cub.2003.10.005

    2002

    • Baum B (2002). Winging It: Actin on the Fly. Developmental Cell, 2, 125 - 133.
    • Wills Z, Emerson M, Rusch J, Bikoff J, Baum B, Perrimon N, Van Vactor D (2002). A Drosophila homolog of cyclase associated proteins collaborates with the Abl tyrosine kinase to control midline axon pathfinding. Neuron, 36(4), 611 - 622.
    • Baum B (2002). Drosophila oogenesis: generating an axis of polarity.. Curr Biol, 12(24), R835 - R837.
    • Baum B (2002). Drosophila oogenesis: generating an axis of polarity. Current Biology, 12(24), 0 - 0.

    2001

    • Baum B, Perrimon N (2001). Spatial control of the actin cytoskeleton in Drosophila epithelial cells. Nature Cell Biology, 3(10), 883 - 890. doi:10.1038/ncb1001-883

    1998

    • BAUM B, Nishitani H, Yanow S, Nurse P (1998). Cdc18 transcription and proteolysis couple S phase to passage through mitosis. EMBO J., 19, 5689 - 5698.

    1997

    • Ink B, Zörnig M, Baum B, Hajibagheri N, James C, Chittenden T, Evan G (1997). Human Bak induces cell death in Schizosaccharomyces pombe with morphological changes similar to those with apoptosis in mammalian cells. Molecular and Cellular Biology, 17(5), 2468 - 2474.
    • BAUM B, Wuarin J, Nurse P (1997). Control of S-phase periodic transcription in the fission yeast mitotic cycle. EMBO J., 15, 4676 - 4688.