Dr Andrea Townsend-Nicholson

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Personal Profile

Name: Andrea Townsend-Nicholson Email: a.townsend-nicholson@ucl.ac.uk
Title: Dr Tel: 020 7679 2237
Department: Structural & Molecular Biology Fax: 020 7679 7193
Position: Senior Lecturer Address: Gower Street, London, London, WC1E 6BT
Research Domain: Basic Life Sciences, Cardiometabolic Science, Neuroscience, Personalised Medicine Web Page: Personal Web Page

Profile

Research Description

I am interested in understanding how extracellular signals are transduced into intracellular responses. My research is strongly focused on enhancing our understanding of the molecular basis of health and disease and my work focuses primarily on elucidating the role of cell surface receptors in these processes. We use molecular biology, biochemistry, cell biology, cellular imaging, cell signalling and molecular pharmacology to study purinergic, glutamatergic, GABAergic and dopaminergic responses in the central and autonomic nervous systems and in the cardiovascular system.  I am also interested in the development of new technologies to help gain a more refined understanding of existing cellular systems and to use these methods, in the case of bioelectrosprays, to generate new tissues and biological structures from individual cells for therapeutic benefit.


Research Activities

Development of Bioelectrosprays for Tissue Engineering

FLIPR Tetra Assay Development

Molecular mechanisms of cell surface receptor function

Purinergic Control of Smooth Muscle

Education Description

 I am the Head of Teaching for Molecular Biosciences, which is responsible for UCL's undergraduate degree programmes in Biochemistry, Biotechnology and Molecular Biology. My teaching interests extend to the development and use of novel technology and assessment methodologies to enhance student learning.  I teach on BIOC1001/1008/1009, BIOC2001, BIOC2008, BIOC3008 and supervise final year undergraduate literature and laboratory-based research projects and MRes and MSc postgraduate research projects.

UCL Collaborators

Dr Suwan Jayasinghe; Dr Brian King; Prof Dimitri Michael Kullmann; Dr Leslie Dale; Dr Stephanie Schorge

External Collaborators

Publications

    2014

    • Lin EJ, Symes CW, Townsend-Nicholson A, Klugmann M, Klugmann CB, Lehnert K, Fong D, Young D, During MJ (2014). An Immunological Approach to Increase the Brain's Resilience to Insults.. ISRN Neurosci, 2014, 103213 - . doi:10.1155/2014/103213

    2010

    • Eddaoudi A, Townsend-Nicholson A, Timms JF, Schorge S, Jayasinghe SN (2010). Molecular characterisation of post-bio-electrosprayed human brain astrocytoma cells. Analyst, 135(10), 2600 - 2612. doi:10.1039/C0AN00213E

    2008

    • Cai Q, Townsend-Nicholson A (2008). Functional characterisation of A1 : P2Y1 heterodimeric receptors. PURINERG SIGNAL, 4, S77 - S78.
    • King BF, Townsend-Nicholson A (2008). Involvement of P2Y1 and P2Y11 purinoceptors in parasympathetic inhibition of colonic smooth muscle.. The Journal of Pharmacology and Experimental Therapeutics, 324(3), 1055 - 1063. doi:10.1124/jpet.107.131169
    • Townsend-Nicholson A, Drew CM, Saunders-Wage E, King BF (2008). Modulation of P2Y11 by compounds with activity at guinea-pig taenia coli.
    • King BF, Townsend-Nicholson A (2008). Benzoyl-benzoyl-ATP: a smooth muscle relaxant and P2Y11 agonist.
    • King BF, Townsend-Nicholson A (2008). α,β-methyleneATP: a P2Y11 agonist and smooth muscle relaxant.
    • King B, Townsend-Nicholson A (2008). Alpha, beta-methylene-ATP: a P2Y11 agonist and smooth muscle relaxant. PURINERG SIGNAL, 4, S131 - S131.
    • Saunders-Wage E, Townsend-Nicholson A (2008). Comparative genomics of the P2Y11 receptor. PURINERG SIGNAL, 4, S7 - S7.

    2007

    • Devader C, Drew CM, Geach TJ, Tabler J, Townsend-Nicholson A, Dale L (2007). A novel nucleotide receptor in Xenopus activates the cAMP second messenger pathway. Federation of European Biochemical Societies Letters, 581(27), 5332 - 5336. doi:10.1016/j.febslet.2007.10.024

    2006

    • Townsend-Nicholson A, Jayasinghe SN (2006). Cell Electrospinning: a unique biotechnique for encapsulating living organisms for generating active biological microthreads/scaffolds.. Biomacromolecules, 7(12), 3364 - 3369. doi:10.1021/bm060649h
    • Jayasinghe SN, Townsend-Nicholson A (2006). Stable electric-field driven cone-jetting of concentrated biosuspensions.. Lab on a Chip, 6(8), 1086 - 1090. doi:10.1039/b606508m
    • King BF, Liu M, Townsend-Nicholson A, Burnstock G (2006). Inhibitory interaction between activated TRPV1 and P2X3 receptors.
    • Jayasinghe SN, Townsend-Nicholson A (2006). Bio-electrosprays: The next generation of electrified jets.. Biotechnology Journal, 1(9), 1018 - 1022. doi:10.1002/biot.200600128

    2005

    • King BF, Liu M, Townsend-Nicholson A, Pfister J, Padilla F, Ford AP, Gever JR, Oglesby IB, Schorge S, Burnstock G (2005). Antagonism of ATP responses at P2X receptor subtypes by the pH indicator dye, Phenol red.. British Journal of Pharmacology, 145(3), 313 - 322. doi:10.1038/sj.bjp.0706187

    2003

    • King BF, Townsend-Nicholson A (2003). Nucleotide and nucleoside receptors. Tocris Reviews, 23, 1 - 12.
    • King BF, Liu M, Brown SG, Knight G, Townsend-Nicholson A, Dunn PM, Wildman SS, Jackson VM, Cunnane TC, Pfister J, Padilla F, Ford AP, Burnstock G (2003). P2X receptor blockade by the pH indicator dye, Phenol Red.
    • Hillman KA, Harada H, Chan CM, Townsend-Nicholson A, Moss SE, Miyamoto K, Suketa Y, Burnstock G, Unwin RJ, Dunn PM (2003). Chicken DT40 cells stably transfected with the rat P2X7 receptor ion channel: a system suitable for the study of purine receptor-mediated cell death. Biochemical Pharmacology, 66(3), 415 - 424.

    2002

    • Townsend-Nicholson A (2002). Molecular interactions of purinoceptor subtypes found on blood platelets. British Society for Cardiovascular Research Bulletin, 15(1), 4 - 11.
    • Brown SG, Townsend-Nicholson A, Jacobson KA, Burnstock G, King BF (2002). Heteromultimeric P2X1/2 receptors show a novel sensitivity to extracellular pH. The Journal of Pharmacology and Experimental Therapeutics, 300(2), 673 - 680. doi:10.1124/jpet.300.2.673
    • King B, Liu M, Brown SG, Knight GE, Townsend-Nicholson A, Dunn PM, Wildman SS, Jackson VM, Cunnana TC, Burnstock G (2002). P2X receptor blockade by the pH indicator dye Phenol Red.

    2001

    • Liu M, King BF, Dunn PM, Rong W, Townsend-Nicholson A, Burnstock G (2001). Coexpression of P2X3 and P2X2 receptor subunits in varying amounts generates heterogenous populations of P2X receptors that evoke a spectrum of agonist responses comparable to that seen in sensory neurons. The Journal of Pharmacology and Experimental Therapeutics, 296(3), 1043 - 1050.
    • King BF, Townsend-Nicholson A (2001). Properties of heteromeric P2X receptors.

    2000

    • King BF, Townsend-Nicholson A, Wildman SS, Thomas T, Spyer KM, Burnstock G (2000). Coexpression of rat P2X2 and P2X6 subunits in Xenopus oocytes. Journal of Neuroscience, 20(13), 4871 - 4877.
    • Hoebertz A, Townsend-Nicholson A, Glass R, Burnstock G, Arnett TR (2000). Expression of P2 receptors in bone and cultured bone cells. Bone, 27(4), 503 - 510.
    • Glass R, Townsend-Nicholson A, Burnstock G (2000). P2 receptors in the thymus: expression of P2X and P2Y receptors in adult rats, an immunohistochemical and in situ hybridisation study. Cell and Tissue Research, 300(2), 295 - 306.
    • Townsend-Nicholson A, Teo J, King BF (2000). Do P2X receptor splice variants play a physiological role?.
    • King BF, Townsend-Nicholson A (2000). Recombinant P2Y receptors: the UCL experience. Journal of the Autonomic Nervous System, 81(1-3), 164 - 170.

    1999

    • Hoebertz A, Townsend-Nicholson A, Burnstock G, Arnett TR (1999). Expression of P2 receptors in bone.
    • Townsend-Nicholson A, King BF, Wildman SS, Burnstock G (1999). Molecular cloning, functional characterization and possible cooperativity between the murine P2X4 and P2X4a receptors.. Brain Res Mol Brain Res, 64(2), 246 - 254.
    • Townsend-Nicholson A, King BF, Wildman SS, Burnstock G (1999). Molecular cloning, functional characterization and possible cooperativity between the murine P2X4 and P2X4a receptors. Molecular Brain Research, 64(2), 246 - 254. doi:10.1016/S0169-328X(98)00328-3
    • Townsend-Nicholson A, King BF, Burnstock G (1999). Possible co-operativity between murine P2X4 and P2X4a receptors..
    • Meyer MP, Clarke JDW, Patel K, Townsend-Nicholson A, Burnstock G (1999). Selective expression of a purinoreceptor, cP2Y1, suggests a role for ucleotide signalling in development of the chick embryo. Developmental Dynamics, 214, 152 - 158.

    1998

    • King BF, Townsend-Nicholson A, Burnstock G (1998). Metabotropic receptors for ATP and UTP: exploring the correspondence between native and recombinant nucleotide receptors. Trends in Pharmacological Sciences, 19, 506 - 514.
    • Burnstock G, King BF, Townsend-Nicholson A (1998). P1 and P2 receptor. Update News-sheet, issue 8.
    • Chan CM, Unwin RJ, Bardini M, Oglesby IB, Ford APDW, Townsend-Nicholson A, Burnstock G (1998). Localization of the P2X1 purinoceptors by autoradiography and immunohistochemistry in rat kidneys. American Journal of Physiology, 274, F799 - 804.
    • Chan CM, Unwin RJ, Bardini M, Oglesby IB, Ford AP, Townsend N, Burnstock G (1998). Localization of P2X1 purinoceptors by autoradiography and immunohistochemistry in rat kidneys. AM J PHYSIOL, 274(4 Pt 2), F799 - F804.
    • de Zwart M, Link R, von Frijtag Drabbe Künzel JK, Cristalli G, Jacobson KA, Townsend-Nicholson A, IJzerman AP (1998). A functional screening of adenosine analogues at the adenosine A2B receptor: a search for potent agonists.. Nucleosides Nucleotides, 17(6), 969 - 985. doi:10.1080/07328319808004215
    • Dalpiaz A, Townsend-Nicholson A, Beukers MW, Schofield PR, IJzerman AP (1998). Thermodynamics of full agonist, partial agonist, and antagonist binding to wild-type and mutant adenosine A1 receptors.. Biochem Pharmacol, 56(11), 1437 - 1445.
    • Camp D, Green JM, Kaiser SM, Moni RW, Townsend-Nicholson A, Schofield PR, Quinn RJ (1998). Diimidazo[1,2-c:4',5'-e]pyrimidines: adenosine agonist activity demonstrated by microphysiometry.. Bioorg Med Chem Lett, 8(6), 691 - 694.
    • King BF, Wildman SS, Townsend-Nicholson A, Burnstock A (1998). Antagonism of an adenosine/ATP receptor in follicular Xenopus oocytes. The Journal of Pharmacology and Experimental Therapeutics, 285, 1005 - 1011.

    1997

    • Townsend-Nicholson A (1997). Approaches to the Stable Transfection of G Protein-Coupled Receptors. In Challiss RAJ (Ed.), Receptor signal transduction protocols (pp. 45 - 54). : Humana Pr Inc.
    • Atkinson MR, Townsend-Nicholson A, Nicholl JK, Sutherland GR, Schofield PR (1997). Cloning, characterisation and chromosomal assignment of the human adenosine A3 receptor (ADORA3) gene.. Neurosci Res, 29(1), 73 - 79.
    • Townsend-Nicholson A (1997). Approaches to the stable transfection of G protein-coupled receptors.. Methods Mol Biol, 83, 45 - 54. doi:10.1385/0-89603-495-X:45

    1996

    • Le F, Townsend-Nicholson A, Baker E, Sutherland GR, Schofield PR (1996). Characterization and chromosomal localization of the human A2a adenosine receptor gene: ADORA2A.. Biochem Biophys Res Commun, 223(2), 461 - 467. doi:10.1006/bbrc.1996.0916

    1995

    • Megson AC, Dickenson JM, Townsend-Nicholson A, Hill SJ (1995). Synergy between the inositol phosphate responses to transfected human adenosine A1-receptors and constitutive P2-purinoceptors in CHO-K1 cells.. Br J Pharmacol, 115(8), 1415 - 1424.
    • Townsend-Nicholson A, Baker E, Sutherland GR, Schofield PR (1995). Localization of the adenosine A2b receptor subtype gene (ADORA2B) to chromosome 17p11.2-p12 by FISH and PCR screening of somatic cell hybrids.. Genomics, 25(2), 605 - 607.
    • Townsend-Nicholson A, Baker E, Schofield PR, Sutherland GR (1995). Localization of the adenosine A1 receptor subtype gene (ADORA1) to chromosome 1q32.1.. Genomics, 26(2), 423 - 425.

    1994

    • Townsend-Nicholson A, Schofield PR (1994). A threonine residue in the seventh transmembrane domain of the human A1 adenosine receptor mediates specific agonist binding.. J Biol Chem, 269(4), 2373 - 2376.

    1992

    • Selbie LA, Townsend-Nicholson A, Iismaa TP, Shine J (1992). Novel G protein-coupled receptors: a gene family of putative human olfactory receptor sequences.. Brain Res Mol Brain Res, 13(1-2), 159 - 163.
    • Townsend-Nicholson A, Shine J (1992). Molecular cloning and characterisation of a human brain A1 adenosine receptor cDNA.. Brain Res Mol Brain Res, 16(3-4), 365 - 370.

    • Pierce KD, Shine J, Selbie L, Furlong T, Townsend-Nicholson (). DNA SEQUENCES ENCODING THE HUMAN A1, A2a and A2b ADENOSINE RECEPTORS.