Prof J. Godovac-Zimmermann's Molecular Cell Dynamics Group uses quantitative changes in cellular proteomes to study parallel dynamic changes in cellular function, primarily in the area of cell signal transduction. We monitor and analyse the spatial and temporal properties of the molecular networks and fluxes involved in living cells by following the linked proteome changes.
New technologies developed in our laboratories and elsewhere, including gel and non-gel protein separations, quantitative shotgun proteomics, high-throughput automatic protein identification, soft ionisation mass spectrometry and bioinformatics enable us to follow imprinting of the cellular proteome by functional processes. We are working on new technologies to increase sensitivity and proteome coverage. We identify proteins and characterize their phenotypic changes using quantitative shotgun LC-ESI Ion Trap Orbitrap MS/MS and MALDI MS. The proteomics and bioinformatics workflows for protein identification, quantitation, localization, validation, functional and pathway analysis available in our group are shown below.