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Molecular and cellular imaging

UCL excels in all aspects of imaging of molecules, cells and tissues. Substantial investment from the JIF/Wellcome Trust programme, Platform Technologies CIF awards, and other sources have provided state-of-the-art facilities, where researchers are interested in events that change in time at the organ, tissue, cell and molecular level using advanced imaging methodologies.

Confocal and Electron Microscopy

Histology

Flow Cytometry and Cell Sorting

Confocal and Electron Microscopy

All cell and tissue imaging core facilities can be contacted by e-mailing microscopy@ucl.ac.uk

	CDB Imaging Biosciences EM Facility	MRC LMCB	WIBR/CI Confocal	Royal Free	ICH Confocal	IoO Imaging	IoN EM Imaging
Confocal	√	√	√	√	√	√
2- or multi- photon	√				√
Live cell imaging	√	√	√	√	√
FRET	√	√			√
FLIM	√
TIRF	√
Spinning disk			√
Laser capture/dissection			√			√
High-Content Screening		√
Transmission EM	√					√	√
Scanning EM	√					√	√
Other EM						Gatan 3-view
Software packages	Imaris	Acapella	Volocity ImageJ	Volocity TILLVision	ImageJ CellProfiler  Volocity Openlab	Imaris Amira
Contact	D.Becker C.Thrasivoulou M.Turmaine	Robin Ketteler	Tomas Adejumo George Chennell	Shane Minogue	Bernard Vernay	Peter Munro	Kerrie Venner

The Eastman Dental Institute has a number of specialist facilities for imaging in addition to confocal microscopy, transmission EM and scanning EM
Atomic Force Microscopy
Attenuated Total Reflectance - Fourier Transform Infrared Spectroscopy
Raman and Fourier Mapping
Goniometry

The London Centre for Nanotechnology (LCN) has a number of specialist facilities in addition to Confocal/Fluorescent Microscopy. These include Atomic Force Microscopy (AFM), Scanning Tunneling Microscopy (STM), and an Ion Beam Facility (FIB).

Training

All facilities offer one-to-one training for users.

Short courses in confocal imaging (Biosciences) are listed here.

Histology

There is a LEICA SCN400 with 384 slide autoloader at IoN Neuropathology, in Sebastian Brandner's group, which can be used by UCL staff for a fee. Slides can be stored for a fee or can be exported and made directly available to the user. In April 2012 a new Histology Facility will open at IoN, for academic research, clinical diagnostics and industry. The main expertise is mouse and human histology of the central nervous system, but facility staff will be able to cut up, embed, section and stain any other tissue.

UCL Advanced Diagnostics has one of the largest collections of antibodies and in situ probes in the country for use on formalin-fixed paraffin embedded tissue sections, and is a major centre for HER2 testing using both immunocytochemistry and fluorescent in situ hybridisation (FISH). UCLAD provides a service to UCL Departments and UCL Hospitals.

Flow Cytometry and Cell Sorting

The Flow Cytometry Core Facility at the UCL Institute of Child Health (ICH) and Great Ormond Street Hospital for Children (GOSH) provides state of the art instrumentation for cell analysis and sorting. Guidelines for the use of the facility and a description of the techniques are provided on these web pages.

The WIBR/Cancer Inst Scientific Support Service has two systems for Flow Cytometry. Both allow the user to measure simultaneously multiple physical characteristics of a single particle (cell) in a moving fluid stream. 

The Division of Infection & Immunity also has FACS facilities. Please contact the
lab manager Jamie Evans for details.

The Eastman Dental Institute has a flow cytometry facility.