Dr Talvinder Sihra

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Name: Talvinder Sihra Email: t.sihra@ucl.ac.uk
Title: Dr Tel: 020 7679 3296
Department: Neuro, Physiology & Pharmacology Fax:
Position: Reader in Cellular and Molecular Neuroscience Address: Medawar Building, Gower Street, London, WC1E 6BT
Research Domain: Basic Life Sciences, Neuroscience Web Page: Personal Web Page

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Research Description

The basic interests of the laboratory centre around the mechanisms by which neurotransmitter release is regulated at central nervous system (CNS) synapses.

1) Presynaptic receptors, through ionotropic and metabotropic mechanisms, represent a fundamental means for regulating neurotransmitter release. One of our interests is to identify and characterize presynaptic receptors that modulate the release of the neurotransmitters glutamate and GABA. The model system we use for these studies is the isolated nerve terminal preparation (synaptosomes). Nerve terminal depolarization leads to Ca2+-influx and exocytosis, followed by endocytosis and recycling of transmitter containing small synaptic vesicles (SSVs). To delineate the loci at which presynaptic receptor activation impinge, we use membrane potential-sensitive dyes to assay nerve terminal excitability and depolarization, fura-2 to monitor Ca2+-influx and on-line enzymatic assays or HPLC to determine the release of glutamate and GABA by the exocytosis of small synaptic vesicles (SSVs).

Post-translational modification of the proteins involved in the cascade of events leading to neurotransmitter release offers a powerful means of mediating presynaptic plasticity. Thus, one way that presynaptic receptor activation can potentially modulate the properties of proteins involved in neurotransmitter release is through the stimulation of second messenger cascades that lead to protein phosphorylation or dephosphorylation. Using synaptosomes labelled with 32P-orthophosphate, we can ascertain presynaptic receptor-mediated activation of specific protein and lipid kinases and phosphatases employing identified intraterminal substrates for these enzymes. Currently, we are characterising the nerve terminal modulatory roles of mitogen-activated protein kinases and lipid kinases leading to the production of polyphosphoinositides.

2) The second major focus of the laboratory is to determine the role of specific protein kinases or phosphotases in the cascade of events leading to SSV exocytosis and endocytosis. For these studies, we have taken the approach of altering enzyme expression in neuronal cell lines and primary cell cultures that are amenable to molecular biological procedures. Currently, we are evaluating the effects of altered expression of the major Ca2+-dependent protein phosphatase, protein phosphatase 2B (calcineurin, CN) and its associated proteins. We wish to characterize of the role of CN in: (a) controlling voltage-dependent Ca2+-influx and, (b) the exocytotic/endocytotic cycling of SSVs and control thereof by the CN substrates, synapsin I and dynamin. We examine VDCC activity using whole-cell patch-clamping and Ca2+-influx, either spectrophometrically or by Ca2+-imaging of single cells, using Ca2+-sensitive fluorophors. Effects of phosphorylation/dephosphorylation on SSV-associated proteins are elucidated using styryl SV-probes (e.g. FM1-43, FM2-10) to image endocytosis/exocytosis.


Research Activities

Modulation of neurotransmitter release: role of cannabinoid signalling and transporters

Regulation of Neurotransmitter Release by Presynaptic Receptor Signalling Mechanisms and Protein Phosphorylation/Dephosphorylation

Education Description

LECTURE (L), PRACTICAL (P) & TUTORIAL (T) TEACHING ON:

PHAR3011 - Synaptic Pharmacology (L & T) |

PHAR3001/3002 - Neuropharmacology (L & P) |

PHOL3006 - Cellular Basis of Brain Function (L) |

PHAR2002/2005 - General and Systematic Pharmacology/Introductory Pharmacology (L & T) |

PHAR2003/2006 - Experimental Pharmacology/Practical Pharmacology (P) |

PHOL1001 - Mammalian Physiology (T) |

MSc Neuroscience (L & T) |

Phase 1 Preclinical MBBS (L, T & P) |



ORGANISATION:

Head of Teaching (Department of Neuroscience, Physiology & Pharmacology)

BSc Pharmacology Programme Tutor |

Chair of BSc Pharmacology Programme Teaching Committee |

Chair of BSc Physiology and Pharmacology Examination Board |

Course Organiser and Tutor for 3rd Year PHAR3011 - Synaptic Pharmacology module |

Organiser & Tutor for 3rd Year Pharmacology Laboratory (PHAR3010) and Library (PHAR3009) Projects |

Course Organiser and Tutor for 2nd Year PHAR2002 - General and Systematic Pharmacology & PHAR2005 - Introductory Pharmacology modules

Co-Course Organiser PHOL1001 - Mammalian Physiology |

UCL Collaborators

Prof Stuart Cull-Candy; Prof Dmitri Rusakov

External Collaborators

Publications

    2014

    • Sihra TS, Flores G, Rodríguez-Moreno A (2014). Kainate receptors: multiple roles in neuronal plasticity.. Neuroscientist, 20(1), 29 - 43. doi:10.1177/1073858413478196

    2013

    • Sihra TS, Rodríguez-Moreno A (2013). Presynaptic kainate receptor-mediated bidirectional modulatory actions: mechanisms.. Neurochem Int, 62(7), 982 - 987. doi:10.1016/j.neuint.2013.03.012
    • Rodríguez-Moreno A, Sihra TS (2013). Presynaptic kainate receptor-mediated facilitation of glutamate release involves Ca2+-calmodulin and PKA in cerebrocortical synaptosomes.. FEBS Lett, 587(6), 788 - 792. doi:10.1016/j.febslet.2013.01.071
    • Andrade-Talavera Y, Duque-Feria P, Sihra TS, Rodríguez-Moreno A (2013). Presynaptic kainate receptor-mediated facilitation of glutamate release involves PKA and Ca(2+) -calmodulin at thalamocortical synapses.. J Neurochem, , - . doi:10.1111/jnc.12310

    2012

    • Andrade-Talavera Y, Duque-Feria P, Negrete-Díaz JV, Sihra TS, Flores G, Rodríguez-Moreno A (2012). Presynaptic kainate receptor-mediated facilitation of glutamate release involves Ca2+ -calmodulin at mossy fiber-CA3 synapses.. J Neurochem, 122(5), 891 - 899. doi:10.1111/j.1471-4159.2012.07844.x

    2011

    • Sihra TS, Rodriguez-Moreno A (2011). Metabotropic Actions of Kainate Receptors in the Control of GABA Release. ADV EXP MED BIOL, 717, 1 - 10.
    • Rodriguez-Moreno A, Sihra TS (2011). Metabotropic Actions of Kainate Receptors in the Control of Glutamate Release in the Hippocampus. ADV EXP MED BIOL, 717, 39 - 48.
    • Porcher C, Hatchett C, Longbottom RE, McAinch K, Sihra TS, Moss SJ, Thomson AM, Jovanovic JN (2011). Positive feedback regulation between gamma-aminobutyric acid type A (GABA(A)) receptor signaling and brain-derived neurotrophic factor (BDNF) release in developing neurons.. J Biol Chem, 286(24), 21667 - 21677. doi:10.1074/jbc.M110.201582
    • Rodríguez-Moreno A, Sihra TS (2011). Kainate receptors. Novel signaling insights.. Adv Exp Med Biol, 717, vii - xiii.
    • Rodriguez-Moreno A, Sihra TS (2011). CONCLUDING REMARKS. ADV EXP MED BIOL, 717, 127 - 127.

    2010

    • Nizami S, Lee VW, Davies J, Long P, Jovanovic JN, Sihra TS (2010). Presynaptic roles of intracellular Ca(2+) stores in signalling and exocytosis.. Biochem Soc Trans, 38(2), 529 - 535. doi:10.1042/BST0380529

    2009

    • Long P, Mercer A, Begum R, Stephens GJ, Sihra TS, Jovanovic JN (2009). Nerve Terminal GABA(A) Receptors Activate Ca2+/Calmodulin-dependent Signaling to Inhibit Voltage-gated Ca2+ Influx and Glutamate Release. J BIOL CHEM, 284(13), 8717 - 8728. doi:10.1074/jbc.M805322200
    • Long P, Mercer A, Begum R, Stephens GJ, Sihra TS, Jovanovic JN (2009). Nerve terminal GABAA receptors activate Ca2+/calmodulin-dependent signalling to inhibit voltage-gated Ca2+ influx and glutamate release.. Journal of Biological Chemistry, 284(13), 8726 - 8737.
    • Simons JP, Al-Shawi R, Minogue S, Waugh MG, Wiedemann C, Evangelou S, Loesch S, Sihra TS, King R, Warner TT, Hsuan JJ (2009). Loss of phosphatidylinositol 4-kinase 2α activity causes late onset degeneration of spinal cord axons. Proceedings of the National Academy of Sciences of the United States of America, 106(28), 11535 - 11539. doi:10.1073/pnas.0903011106

    2008

    • Brown DA, Sihra TS (2008). Presynaptic signaling by heterotrimeric G-proteins.. In Südhof TC, Starke K (Ed.), Pharmacology of Neurotransmitter Release (pp. 207 - 260). : Springer. doi:10.1007/978-3-540-74805-2
    • Brown DA, Sihra TS (2008). Presynaptic signaling by heterotrimeric G-proteins.. Handb Exp Pharmacol, (184), 207 - 260. doi:10.1007/978-3-540-74805-2_8

    2007

    • Rodriguez-Moreno A, Sihra TS (2007). Kainate receptors with a metabotropic modus operandi.. Trends in Neuroscience, 30(12), 630-7. - 637. doi:10.1016/j.tins.2007.10.001
    • Rodriguez-Moreno A, Sihra TS (2007). Metabotropic actions of kainate receptors in the CNS. Journal of Neurochemistry, 103(6), 2121 - 2135. doi:10.1111/j.1471-4159.2007.04924.x
    • Negrete-Diaz J, Sihra TS, Delgado-García J, Rodriguez-Moreno A (2007). Kainate receptor-mediated presynaptic inhibition converges with presynaptic inhibition mediated by Group II mGluRs and long-term depression at the hippocampal mossy fiber-CA3 synapse.. Journal of Neural Transmission, 114(11), 1425 - 1431. doi:10.1007/s00702-007-0750-4
    • Xu X, Muller-Taubenberger A, Adley KE, Pawolleck N, Lee VY, Wiedemann C, Sihra TS, Maniak M, Jin T, Williams RSB (2007). Attenuation of Phospholipid Signaling Provides a Novel Mechanism for the Action of Valproic Acid.. Eukaryotic Cell, 6(6), 899 - 906. doi:10.1128/EC.00104-06
    • Negrete-Diaz JV, Sihra TS, Delgado-Garcia JM, RodriGuez-Moreno A (2007). Kainate receptor-mediated inhibition of glutamate release involves protein kinase A in the mouse hippocamplis.

    2006

    • Martin SW, Butcher AJ, Berrow NS, Richards MW, Paddon RE, Turner DJ, Dolphin AC, Sihra TS, Fitzgerald EM (2006). Phosphorylation sites on calcium channel alpha1 and beta subunits regulate ERK-dependent modulation of neuronal N-type calcium channels.. Cell Calcium, 39(3), 275 - 292. doi:10.1016/j.ceca.2005.11.002
    • Wang SJ, Wang KY, Wang WC, Sihra TS (2006). Unexpected inhibitory regulation of glutamate release from rat cerebrocortical nerve terminals by presynaptic 5-hydroxytryptamine-2A receptors. Journal of Neuroscience Research, 84(7), 1528 - 1542. doi:10.1002/jnr.21060
    • Negrete-Diaz JV, Sihra TS, Delgado-Garcia JM, Rodriguez-Moreno A (2006). Kainate receptor-mediated inhibition of glutamate release involves protein kinase A in the mouse hippocampus. Journal of Neurophysiology, 96(4), 1829 - 1837. doi:10.1152/jn.00280.2006

    2004

    • Wang SJ, Sihra TS (2004). Noncompetitive metabotropic glutamate5 receptor antagonist (E)-2-methyl-6-styryl-pyridine (SIB1893) depresses glutamate release through inhibition of voltage-dependent Ca2+ entry in rat cerebrocortical nerve terminals (synaptosomes). The Journal of Pharmacology and Experimental Therapeutics, 309(3), 951 - 958.
    • Rodriguez-Moreno A, Sihra TS (2004). Presynaptic kainate receptor facilitation of glutamate release involves protein kinase A in the rat hippocampus. The Journal of Physiology, 557(Pt 3), 733 - 745.

    2003

    • Wang SJ, Sihra TS (2003). Opposing facilitatory and inhibitory modulation of glutamate release elicited by cAMP production in cerebrocortical nerve terminals (synaptosomes). Neuropharmacology, 44(5), 686 - 697. doi:10.1016/S0028-3908(03)00045-5

    2002

    • Wang SJ, Coutinho V, Sihra TS (2002). Presynaptic cross-talk of beta-adrenoreceptor and 5-hydroxytryptamine receptor signalling in the modulation of glutamate release from cerebrocortical nerve terminals. British Journal of Pharmacology, 137(8), 1371 - 1379.

    2001

    • Wang SJ, Sihra TS, Gean PW (2001). Lamotrigine inhibition of glutamate release from isolated cerebrocortical nerve terminals (synaptosomes) by suppression of voltage-activated calcium channel activity. NeuroReport, 12(10), 2255 - 2258.
    • Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Greengard P, Czernik AJ (2001). Opposing Changes in Phosphorylation of Specific Sites in Synapsin I During Ca2-Dependent Glutamate Release in Isolated Nerve Terminals. Journal of Neuroscience, 21(20), 7944 - 7963.

    2000

    • Jovanovic JN, Czernik AJ, Fienberg AA, Greengard P, Sihra TS (2000). Synapsins as mediators of BDNF-enhanced neurotransmitter release. Nature Neuroscience, 3(4), 323 - 329. doi:10.1038/73888
    • Burley JR, Sihra TS (2000). A modulatory role for protein phosphatase 2B (calcineurin) in the regulation of Ca2+ entry. European Journal of Neuroscience, 12(8), 2881 - 2891.
    • Burley JR, Jeromin A, Robertson B, Roder JC, Sihra TS (2000). Overexpression of frequenin in NG108-15 cells increases low voltage-activated calcium channel activity. BIOPHYS J, 78(1), 104A - 104A.

    1999

    • Perkinton MS, Sihra TS (1999). A high-affinity presynaptic kainate-type glutamate receptor facilitates glutamate exocytosis from cerebral cortex nerve terminals (synaptosomes). Neuroscience, 90(4), 1281 - 1292.
    • Perkinton MS, Sihra TS, Williams RJ (1999). Ca2+-permeable AMPA receptors induce phosphorylation of cAMP response element-binding protein through a phosphatidylinositol 3-kinase-dependent stimulation of the mitogen-activated protein kinase signaling cascade in neurons. Journal of Neuroscience, 19(14), 5861 - 5874.

    1998

    • Wiedemann C, Schafer T, Burger MM, Sihra TS (1998). An essential role for a small synaptic vesicle-associated phosphatidylinositol 4-kinase in neurotransmitter release. Journal of Neuroscience, 18(15), 5594 - 5602.
    • Lukyanetz EA, Piper TP, Sihra TS (1998). Calcineurin involvement in the regulation of high threshold Ca channels in NG108-15 (rodent neuroblastoma x glioma hybrid) cells. The Journal of Physiology, 510(2), 371 - 385.
    • Perkinton MS, Sihra TS (1998). Presynaptic GABAB receptor modulation of glutamate exocytosis from rat cerebrocortical nerve terminals: receptor decoupling by protein kinase C. Journal of Neurochemistry, 70(4), 1513 - 1522.

    1997

    • Sihra TS, Hemmings HC (1997). Posttranslational Modifications: Techniques and Protocols. In Boulton AA, Baker GB (Ed.), Neuromethods (pp. 67 - 119). : Humana Press.
    • Sihra TS (1997). Protein phosphorylation and dephosphorylation in isolated nerve terminals (synaptosomes). In Hemmings Jr HC (Ed.), Regulatory Protein Modifications: Techniques and Protocols (pp. 67 - 119). : Humana Press.

    1996

    • Lukyanetz EA, Piper TP, Dolphin AC, Sihra TS (1996). Interaction between calcium channels and calcineurin in NG108-15 cells.
    • Perkinton MS, Sihra TS (1996). omega-Conotoxin MVIIC reversibly inhibits high-K+- and 4-aminopyridine-evoked glutamate release from isolated nerve terminals (synaptosomes). BRIT J PHARMACOL, 117, P63 - P63.
    • Jovanovic JN, Benfenati F, Siow YL, Sihra TS, Sanghera JS, Pelech SL, Greengard P CAJ (1996). Neurotrophins stimulate phosphorylation of synapsin I by MAP kinase and regulate synapsin I-actin interactions. Proceedings of the National Academy of Sciences of the United States of America, 16(93(8)), 3679 - 3683.
    • Jovanovic JN, Benfenati F, Siow YL, Sihra TS, Sanghera JS, Pelech SL, Greengard P, Czernik AJ (1996). Neurotrophins stimulate phosphorylation of synapsin I by MAP kinase and regulate synapsin I-actin interactions.. Proc Natl Acad Sci U S A, 93(8), 3679 - 3683.
    • Jovanovic JN, Sihra TS, Nairn AC, Greengard P, Czernik AJ (1996). Regulation of MAP kinase activity and phosphorylation of synapsin I in presynaptic nerve terminals.. MOL BIOL CELL, 7, 3805 - 3805.

    1995

    • Nairn AC, Sihra TS, Andjus P, Craig A-M, Miyawaki A, Kloppenburg P, Lin Z, Pouzat C (1995). Rapid purification of protein phosphatase-2B (calcineurin) from rat forebrain. Neuroprotocols, 6, 105 - 107.
    • Nairn AC, Sihra TS, Ebens A, Galagan J, Greene K, Hettinger-Smith B (1995). Phosphorylation of phosphatase inhibitor-1 by ATP--S and dephosphorylation of thiophosphorylated inhibitor by protein phosphatases. Neuroprotocols, 6, 108 - 111.
    • Li L, Chin LS, Shupliakov O, Brodin L, Sihra TS, Hvalby O, Jensen V, Zheng D, McNamara JO, Greengard P (1995). Impairment of synaptic vesicle clustering and of synaptic transmission, and increased seizure propensity, in synapsin I-deficient mice.. Proc Natl Acad Sci U S A, 92(20), 9235 - 9239.
    • Li L, Chin , L S, Shupliakov , O , Brodin , L , Sihra , T S, Hvalby , O , Jensen , V , Zheng , D , McNamara , J O, Greengard , P , Andersen , P (1995). Impairment of synaptic vesicle clustering and of synaptic transmission, and increased seizure propensity, in synapsin I-deficient mice.. Proceedings of the National Academy of Sciences of the United States of America, 26(92(20)), 9235 - 9239.
    • Pearson HA, Sihra TS (1995). Ca/calmodulin-dependent kinase II inhibitor KN62 attenuates glutamate release by inhibiting voltage-dependent Ca(2+)-channels. Neuropharmacology, 34(7), 731 - 741.
    • Sihra TS, Nairn AC, Kloppenburg P, Lin Z, Pouzat C (1995). A role for calcineurin (protein phosphatase-2B) in the regulation of glutamate release. Biochemical and Biophysical Research Communications, 212(2), 609 - 616.

    1994

    • Coffey ET, Herrero I, Sihra TS, Sanchez-Prieto J, Nicholls DG (1994). Glutamate exocytosis and MARCKS phosphorylation are enhanced by a metabotropic glutamate receptor coupled to a protein kinase C synergistically activated by diacylglycerol and arachidonic acid.. Journal of Neurochemistry, 63(4), 1303 - 1310.

    1993

    • COFFEY ET, HERRERO I, SIHRA TS, NICHOLLS DG (1993). METABOTROPIC RECEPTOR ACTIVATION OF GLUTAMATE RELEASE IS PKC MEDIATED. J NEUROCHEM, 61, S253 - S253.
    • Benfenati F, Valtorta F, Rossi MC, Onofri F, Sihra T, Greengard P (1993). Interactions of synapsin I with phospholipids: possible role in synaptic vesicle clustering and in the maintenance of bilayer structures. The Journal of Cell Biology, 123(6 Pt 2), 1845 - 1855.
    • Sihra TS, Piomelli D, Nichols RA (1993). Barium evokes glutamate release from rat brain synaptosomes by membrane depolarization: involvement of K+, Na+, and Ca2+ channels. Journal of Neurochemistry, 61(4), 1220 - 1230.
    • Sihra T (1993). Glutamate release from isolated nerve terminals: modulatory role of protein phosphorylation and dephosphorylation. Biochemical Society Transactions, 21(2), 410 - 416.
    • Coffey ET, Sihra TS, Nicholls DG (1993). Protein kinase C and the regulation of glutamate exocytosis from cerebrocortical synaptosomes. Journal of Biological Chemistry, 268(28), 21060 - 21065.
    • Sihra T, Nichols RA (1993). Mechanisms in the regulation of neurotransmitter release from brain nerve terminals: current hypotheses. Neurochemical Research, 18(1), 47 - 58.
    • COFFEY ET, HERRERO I, SANCHEZPRIETO J, SIHRA TS, NICHOLLS DG (1993). METABOTROPIC RECEPTOR ACTIVATION OF GLUTAMATE RELEASE IS PKC MEDIATED. J NEUROCHEM, 61, S17 - S17.

    1992

    • Sihra TS, Bogonez E, Nicholls DG (1992). Localized Ca2+ entry preferentially effects protein dephosphorylation, phosphorylation, and glutamate release. Journal of Biological Chemistry, 267(3), 1983 - 1989.

    1991

    • Barrie AP, Nicholls DG, Sanchez-Prieto J, Sihra TS (1991). An ion channel locus for the protein kinase C potentiation of transmitter glutamate release from guinea pig cerebrocortical synaptosomes. Journal of Neurochemistry, 57(4), 1398 - 1404.

    1990

    • Nichols RA, Sihra TS, Czernik AJ, Nairn AC, Greengard P (1990). Calcium/calmodulin-dependent protein kinase II increases glutamate and noradrenaline release from synaptosomes. Nature, 343(6259), 647 - 651.

    1989

    • Piomelli D, Wang JK, Sihra TS, Nairn AC, Czernik AJ, Greengard P (1989). Inhibition of Ca2+/calmodulin-dependent protein kinase II by arachidonic acid and its metabolites. Proceedings of the National Academy of Sciences of the United States of America, 86(21), 8550 - 8554.
    • Wang JK, Walaas SI, Sihra TS, Aderem A, Greengard P (1989). Phosphorylation and associated translocation of the 87-kDa protein, a major protein kinase C substrate, in isolated nerve terminals. Proceedings of the National Academy of Sciences of the United States of America, 86(7), 2253 - 2256.
    • Sihra TS, Wang JK, Gorelick FS, Greengard P (1989). Translocation of synapsin I in response to depolarization of isolated nerve terminals. Proceedings of the National Academy of Sciences of the United States of America, 86(20), 8108 - 8112.
    • Cidon S, Sihra TS (1989). Characterization of a H+-ATPase in rat brain synaptic vesicles. Coupling to L-glutamate transport. Journal of Biological Chemistry, 264(14), 8281 - 8288.

    1987

    • Nicholls DG, Sihra TS, Sanchez-Prieto J (1987). Calcium-dependent and -independent release of glutamate from synaptosomes monitored by continuous fluorometry. Journal of Neurochemistry, 49(1), 50 - 57.
    • Sanchez-Prieto J, Sihra TS, Evans D, Ashton A, Dolly JO, Nicholls DG (1987). Botulinum toxin A blocks glutamate exocytosis from guinea-pig cerebral cortical synaptosomes. European Journal of Biochemistry, 165(3), 675 - 681.
    • Sanchez-Prieto J, Sihra TS, Nicholls DG (1987). Characterization of the exocytotic release of glutamate from guinea-pig cerebral cortical synaptosomes. Journal of Neurochemistry, 49(1), 58 - 64.
    • Nicholls DG, Sihra TS, Sanchez-Prieto J (1987). The role of the plasma membrane and intracellular organelles in synaptosomal calcium regulation. Society General Physiogists Series, 42, 31 - 43.
    • Kauppinen RA, Sihra TS, Nicholls DG (1987). Aminooxyacetic acid inhibits the malate-aspartate shuttle in isolated nerve terminals and prevents the mitochondria from utilizing glycolytic substrates. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 930(2), 173 - 178.
    • Sihra TS, Nicholls DG (1987). 4-Aminobutyrate can be released exocytotically from guinea-pig cerebral cortical synaptosomes. Journal of Neurochemistry, 49(1), 261 - 267.

    1986

    • KAUPPINEN RA, SIHRA TS, NICHOLLS DG (1986). THE ROLE OF EXTERNAL CA2+ IN SYNAPTOSOMAL MEMBRANE BIOENERGETICS. BIOCHEM SOC T, 14(5), 889 - 890.
    • Kauppinen RA, Sihra TS, Nicholls DG (1986). Divalent cation modulation of the ionic permeability of the synaptosomal plasma membrane. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 860(2), 178 - 184.
    • SIHRA TS, NICHOLLS DG (1986). CA2+-INDEPENDENT AND CA2+-DEPENDENT GAMMA-AMINOBUTYRATE RELEASE OCCUR FROM DISTINCT COMPARTMENTS WITHIN GUINEA-PIG CEREBRAL CORTICAL SYNAPTOSOMES. BIOCHEM SOC T, 14(5), 909 - 910.
    • Nicholls DG, Sihra TS (1986). Synaptosomes possess an exocytotic pool of glutamate. Nature, 321(6072), 772 - 773.

    1985

    • SIHRA TS, NICHOLLS DG (1985). CA-2+-DEPENDENT RELEASE OF ENDOGENOUS GAMMA-AMINOBUTYRIC ACID FROM ISOLATED NERVE-ENDINGS. BIOCHEM SOC T, 13(5), 919 - 920.

    1984

    • Sihra TS, Scott IG, Nicholls DG (1984). Ionophore A23187, verapamil, protonophores, and veratridine influence the release of gamma-aminobutyric acid from synaptosomes by modulation of the plasma membrane potential rather than the cytosolic calcium. Journal of Neurochemistry, 43(6), 1624 - 1630.

    1981

    • BJORNSSON OG, MURPHY R, SIHRA TS, CHADWICK VS (1981). MEASUREMENT OF POLYETHYLENE-GLYCOL (PEG) IN BIOLOGICAL-FLUIDS - EFFECT OF STORAGE AND FREEZE-THAWING. CLIN RES, 29(2), A304 - A304.

    1980

    • BJORNSSON OG, SIHRA TS, FLETCHER D, CHADWICK VS (1980). DUODENAL PERFUSION WITH SODIUM TAUROCHOLATE SELECTIVELY INHIBITS BILIARY BUT NOT PANCREATIC-SECRETION IN MAN. GASTROENTEROLOGY, 78(5), 1142 - 1142.

    1979

    • BJORNSSON OG, FLETCHER D, SIHRA TS, CAMILLERI M, CHADWICK VS (1979). DUODENAL PERFUSION OF SODIUM TAUROCHOLATE (STC) IN MAN, SELECTIVITY INHIBITS BILIARY BUT NOT PANCREATIC-SECRETION. GUT, 20(10), A925 - A926.