Dr Guy Moss
My laboratory is interested in the biophysical analysis of
physiology. Our work can be divided into several areas. First, we aim to
understand the molecular composition, pharmacology and physiological role of
potassium channels. We are currently focusing our efforts on calcium-activated
potassium channels (channels that open in response to an increase in
intracellular calcium). Mammalian genes have been identified for several of
these proteins including those producing large, small and intermediate
conductance channels. A major challenge in this area is to be able to understand
the properties of native channels by studying their cloned counterparts. A
second and related theme for my laboratory is in trying to understand the control of neuronal signalling. Our work here includes a collaboration with Matthew Whim's group at Louisiana State University in studying peptide secretion. We have also been developing the use of scanning ion conductance microscopy for studying synaptic transmission. We collaborate with groups both at Imperial (Professor Yuri Korchev) and Cambridge (Professor David Klenerman) in the application of this technique. Finally, using biophysical and
electrophysiological techniques we have recently begun to use systems
modelling to examine disease and health, in collaboration with Paola Vergani, Emma Baker, Nick Simmonds and Vivek Dua.
Quantitative approaches to investigate Cystic Fibrosis
The pharmacology and physiological roles of potassium channels The control of neuropeptide secretion and the application of new biophysical techniques
I am one of the Co-Directors of the CoMPLEX MRes/PhD programme, UCLs physical sciences/lifes sciences interface center. I am also the 1st year tutor in Pharmacology and I run the the 1st year pharmacology course PHAR1001. I also teach on a range of 2nd and 3rd year courses, including the preclinical training of medical students.
Prof Stephen Hart; Dr Vivek Dua; Dr Paola Vergani; Prof David Selwood
- Caldwell M, Del Linz SJL, Smart TG, Moss GWJ (2013). Estimating the Geometry of Scanning Ion Conductance Microscope Pipettes from Resistance Variation with Breakage.
- O'Donoghue DL, Dua V, Moss GW, Vergani P (2013). Increased apical Na+ permeability in cystic fibrosis is supported by a quantitative model of epithelial ion transport.. J Physiol, 591(Pt 15), 3681 - 3692. doi:10.1113/jphysiol.2013.253955
- Caldwell M, Del Linz SJL, Smart TG, Moss GWJ (2012). Method for Estimating the Tip Geometry of Scanning Ion Conductance Microscope Pipets. Analytical Chemistry, 84, 8980 - 8984.
- Novak P, Li C, Shevchuk AI, Stepanyan R, Caldwell M, Hughes S, Smart TG, Gorelik J, Ostanin VP, Lab MJ, Moss GWJ, Frolenkov GI, Klenerman D, Korchev YE (2009). Nanoscale live-cell imaging using hopping probe ion conductance microscopy. NAT METHODS, 6(4), 279 - 281. doi:10.1038/NMETH.1306
- Duguid IC, Pankratov Y, Moss GWJ, Smart TG (2007). Somatodendritic Release of Glutamate Regulates Synaptic Inhibition in Cerebellar Purkinje Cells via Autocrine mGluR1 Activation. Journal of Neuroscience, 27(46), 12464 - 12474. doi:10.1523/JNEUROSCI.0178-07.2007
- Siu SC, Boushaba R, Topoyassakul V, Graham A, Choudhury S, Moss G, Titchener-Hooker NJ (2006). Visualising fouling of a chromatographic matrix using confocal scanning laser microscopy.. Biotechnology and Bioengineering, 95(4), 714 - 720. doi:10.1002/bit.21028
- Gorelik JV, Benton DCH, Monaghan A, Lab MJ, Vodyanoy I, Klenerman D, Moss GWJ, Korchev YE (2005). Studying of neuronal synapses by scanning ion conductance microscopy.
- Bahia PK, Suzuki R, Benton DCH, Jowett AJ, Chen M, Trezise DJ, Dickenson AH, Moss GWJ (2005). A Functional Role for Small-Conductance Calcium-Activated Potassium Channels in Sensory Pathways Including Nociceptive Processes.. Journal of Neuroscience, 25, 3489 - 3498. doi:10.1523/JNEUROSCI.0597-05.2005
- Whim MD, Moss GWJ (2004). Measurement of neuropeptide release and dense core granule fusion. Methods, 33(4), 265 - 266.
- Whim MD, Moss GWJ (2004). FMRFamide tagging--how an ionotropic receptor can be used to measure peptide secretion. Methods, 33(4), 295 - 301.
- Monaghan AS, Benton DC, Bahia PK, Hosseini R, Shah YA, Haylett DG, Moss GWJ (2004). The SK3 subunit of small conductance Ca2+-activated K+ channels interacts with both SK1 and SK2 subunits in a heterologous expression system. Journal of Biological Chemistry, 279, 1003 - 1009. doi:10.1074/jbc.M308070200
- Benton DCH, Monaghan AS, Hosseini R, Bahia PK, Haylett DG, Moss GWJ (2003). Small conductance Ca2+-activated K+ channels formed by the expression of rat SK1 and SK2 genes in HEK 293 cells.. The Journal of Physiology, 553, 13 - 19. doi:10.1113/jphysiol.2003.054551
- Gorelik J, Gu Y, Spohr HA, Shevchuk AI, Lab MJ, Harding SE, Edwards CRW, Whitaker M, Moss GWJ, Benton DCH, Sanchez D, Darszon A, Vodyanoy I, Klenerman D, Korchev YE (2002). Ion channels in small cells and subcellular structures can be studied with a smart patch-clamp system. Biophysical Journal, 83, 3296 - 3303.
- Hosseini R, Benton DCH, Dunn PM, Jenkinson DH, Moss GWJ (2001). SK3 is an important component of K+ channels mediating the afterhyperpolarizationin cultured rat SCG neurones. The Journal of Physiology, 535, 323 - 334.
- Whim MD, Moss GWJ (2001). A novel technique that measures peptide secretion on a millisecond timescale reveals rapid changes in release. Neuron, 30, 37 - 50. doi:10.1016/S0896-6273(01)00261-6
- Favre I, Moss GWJ, Goldenberg DP, Otlewski J, Moczydlowski E (2000). Structure-activity relationships for the interaction of bovine pancreatic trypsin inhibitor with an intracellular site on a large conductance Ca2+-activated K+ channel. Biochemistry, 39, 2001 - 2012.
- Favre I, Moss GWJ, Otlewski J, Moczydlowski E (1999). Defining the site of interaction between BPTI and a large conductance Ca-activated K channel. BIOPHYS J, 76(1), A149 - A149.
- Hosseini R, Benton DCH, Haylett DG, Moss GWJ (1999). Cloning of an SK channel from rat sympathetic neurones.