Prof Annette Dolphin

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Personal Profile

Name: Annette Dolphin Email: a.dolphin@ucl.ac.uk
Title: Prof Tel: 020 7679 3276
Department: Neuroscience, Physiology & Pharmacology Fax: 020 7 679 0042
Position: Professor of Pharmacology Address: Andrew Huxley Building, UCL South Cloisters, Gower Street, London, WC1E 6BT
Research Domain: Basic Life Sciences, Neuroscience Web Page: Personal Web Page

Profile

Research Description

My group has expertise in the field of neuronal voltage-dependent calcium channels, and our current work has particular reference to the role of accessory subunits, beta and alpha2delta. We are examining how these subunits modulate the channels and how these interactions are affected in disease states or by drugs.
Some specific current projects include:
Regulation of alpha2delta subunit processing, expression and trafficking under normal and pathological conditions, including neuropathic pain.
Mechanism of action of gabapentin and related drugs, that bind to alpha2delta subunits. Consequences of the calcium channel mutation in the alpha2delta-2 subunit in the mouse mutant Ducky that has absence epilepsy and cerebellar ataxia.
Calcium channel trafficking in neurons and role of the beta subunit.
Mechanism of dominant negative suppression of expression by truncated calcium channel alpha1-subunits such as those resulting from episodic ataxia-2 mutations.
Mechanisms of G protein modulation of calcium channels.

Keywords:
Calcium, Ion channels, G-protein coupled receptors, Neuron, Pain, Epilepsy, Pharmacology, Protein misfolding

Conditions: Ataxias, Epilepsy , Pain - chronic, neuropathic

Methods: Electrophysiological recording techniques, fluorescence microscopy techniques, Gene expression profiling - tissue level, Image analysis, Immunohistochemistry, Intracellular recording, light microscopic techniques, Patch-clamp recording, Protein purification , Proteomics, Cell culture, Anti-sense and morpholino approaches

 

Research Activities

Voltage gated calcium channels

Education Description

My teaching responsibilities include lectures, tutorials and seminars for undergraduate Pharmacology, Biomedical Science and Neuroscience students, medical students and MSc Neuroscience students. 
Pharmacology and Neuroscience undergraduate and MSc students do practical projects in my laboratory. 
I am also Chair of Exam board for Pharmacology and for MSc Neuroscience.

UCL Collaborators

External Collaborators

Publications

    2014

    • Cassidy JS, Ferron L, Kadurin I, Pratt WS, Dolphin AC (2014). Functional exofacially tagged N-type calcium channels elucidate the interaction with auxiliary α2δ-1 subunits.. Proc Natl Acad Sci U S A, , - . doi:10.1073/pnas.1403731111
    • Cassidy JS, Dolphin AC (2014). Using Exofacially Tagged Functional Cav2.2 to Investigate the Modulation of Pore Subunit Trafficking by Auxiliary Calcium Channel Subunits.
    • Dolphin AC (2014). Regulation of Voltage-Gated Calcium Channel Trafficking and Function by Auxiliary Subunits.
    • Ferron L, Nieto-Rostro M, Cassidy JS, Dolphin AC (2014). Fragile X mental retardation protein controls synaptic vesicle exocytosis by modulating N-type calcium channel density.. Nat Commun, 5, 3628 - . doi:10.1038/ncomms4628
    • Nieto-Rostro M, Sandhu G, Bauer CS, Jiruska P, Jefferys JG, Dolphin AC (2014). Altered expression of the voltage-gated calcium channel subunit α2δ-1: a comparison between two experimental models of epilepsy and a sensory nerve ligation model of neuropathic pain.. Neuroscience, , - . doi:10.1016/j.neuroscience.2014.03.013

    2013

    • Azizan EAB, Poulsen H, Tuluc P, Zhou J, Clausen MV, Lieb A, Maniero C, Garg S, Bochukova EG, Zhao W, Shaikh LH, Brighton CA, Teo AED, Davenport AP, Dekkers T, Tops B, Kuesters B, Ceral J, Yeo GSH, Neogi SG, McFarlane I, Rosenfeld N, Marass F, Hadfield J, Margas W, Chaggar K, Solar M, Deinum J, Dolphin AC, Farooqi IS, Striessnig J, Nissen P, Brown MJ (2013). Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension. NATURE GENETICS, 45(9), 1055 - +. doi:10.1038/ng.2716
    • Dolphin AC (2013). The α2δ subunits of voltage-gated calcium channels.. Biochim Biophys Acta, 1828(7), 1541 - 1549. doi:10.1016/j.bbamem.2012.11.019
    • Lana B, Schlick B, Martin S, Pratt WS, Page KM, Goncalves L, Rahman W, Dickenson AH, Bauer CS, Dolphin AC (2013). Differential up-regulation in DRG neurons of an α2δ-1 splice variant with a lower affinity for gabapentin after peripheral sensory nerve injury.. Pain, , - . doi:10.1016/j.pain.2013.12.001
    • Patel R, Bauer CS, Nieto-Rostro M, Margas W, Ferron L, Chaggar K, Crews K, Ramirez JD, Bennett DLH, Schwartz A, Dickenson AH, Dolphin AC (2013). α2δ-1 gene deletion affects somatosensory neuron function and delays mechanical hypersensitivity in response to peripheral nerve damage. The Journal of Neuroscience, , - .
    • Alvarez-Laviada A, Kadurin I, Senatore A, Chiesa R, Dolphin AC (2013). The inhibition of functional expression of calcium channels by prion protein demonstrates competition with α2δ for GPI-anchoring pathways.. Biochem J, , - . doi:10.1042/BJ20131405

    2012

    • Kadurin I, Alvarez-Laviada A, Ng SF, Walker-Gray R, D'Arco M, Fadel MG, Pratt WS, Dolphin AC (2012). Calcium currents are enhanced by α2δ-1 lacking its membrane anchor.. J Biol Chem, 287(40), 33554 - 33566. doi:10.1074/jbc.M112.378554
    • D'Arco M, Dolphin AC (2012). L-type calcium channels: on the fast track to nuclear signaling.. Sci Signal, 5(237), pe34 - . doi:10.1126/scisignal.2003355
    • Dolphin AC (2012). Calcium channel auxiliary α2δ and β subunits: trafficking and one step beyond.. Nat Rev Neurosci, 13(8), 542 - 555. doi:10.1038/nrn3311
    • Kadurin I, Bauer C, Lana B, Alvarez-Laviada A, Nieto-Rostro M, Douglas L, Troeberg L, Nagase H, Dolphin AC (2012). Voltage-Gated Calcium Channel alpha(2)delta Subunits in Lipid Rafts: The Importance of Proteolytic Cleavage Into alpha(2) and delta. BIOPHYSICAL JOURNAL, 102(3), 125A - 125A.
    • Hoppa MB, Lana B, Margas W, Dolphin AC, Ryan TA (2012). alpha 2 delta expression sets presynaptic calcium channel abundance and release probability. NATURE, 486(7401), 122 - U149. doi:10.1038/nature11033
    • Senatore A, Colleoni S, Verderio C, Restelli E, Morini R, Condliffe SB, Bertani I, Mantovani S, Canovi M, Micotti E, Forloni G, Dolphin AC, Matteoli M, Gobbi M, Chiesa R (2012). Mutant PrP Suppresses Glutamatergic Neurotransmission in Cerebellar Granule Neurons by Impairing Membrane Delivery of VGCC alpha(2)delta-1 Subunit. NEURON, 74(2), 300 - 313. doi:10.1016/j.neuron.2012.02.027
    • Hendrich J, Bauer CS, Dolphin AC (2012). Chronic pregabalin inhibits synaptic transmission between rat dorsal root ganglion and dorsal horn neurons in culture.. Channels (Austin), 6(2), 124 - 132. doi:10.4161/chan.19805

    2011

    • Waithe D, Ferron L, Page KM, Chaggar K, Dolphin AC (2011). Beta-subunits promote the expression of Ca(V)2.2 channels by reducing their proteasomal degradation.. J Biol Chem, 286(11), 9598 - 9611. doi:10.1074/jbc.M110.195909
    • Huang Z, Lujan R, Kadurin I, Uebele VN, Renger JJ, Dolphin AC, Shah MM (2011). Presynaptic HCN1 channels regulate Cav3.2 activity and neurotransmission at select cortical synapses.. Nat Neurosci, 14(4), 478 - 486. doi:10.1038/nn.2757
    • Bannister K, Sikandar S, Bauer CS, Dolphin AC, Porreca F, Dickenson AH (2011). Pregabalin suppresses spinal neuronal hyperexcitability and visceral hypersensitivity in the absence of peripheral pathophysiology.. Anesthesiology, 115(1), 144 - 152. doi:10.1097/ALN.0b013e31821f6545
    • Waithe D, Ferron L, Dolphin AC (2011). Stargazin-related protein γ₇ is associated with signalling endosomes in superior cervical ganglion neurons and modulates neurite outgrowth.. J Cell Sci, 124(Pt 12), 2049 - 2057. doi:10.1242/jcs.084988

    2010

    • Dolphin AC (2010). Calcium channel alpha(2)delta subunits in epilepsy and as targets for antiepileptic drugs. EPILEPSIA, 51, 82 - 82. doi:10.1111/j.1528-1167.2010.02868.x
    • Hendrich J, Dolphin AC (2010). Effect of Pregabalin on Synaptic Transmission in Rat Dorsal Horn Ganglion and Dorsal Horn Co-Cultures. BIOPHYSICAL JOURNAL, 98(3), 517A - 517A.
    • Page KM, Heblich F, Margas W, Pratt WS, Nieto-Rostro M, Chaggar K, Sandhu K, Davies A, Dolphin AC (2010). N terminus is key to the dominant negative suppression of Ca(V)2 calcium channels: implications for episodic ataxia type 2.. J Biol Chem, 285(2), 835 - 844. doi:10.1074/jbc.M109.065045
    • Davies A, Kadurin I, Alvarez-Laviada A, Douglas L, Nieto-Rostro M, Bauer CS, Pratt WS, Dolphin AC (2010). The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a posttranslational modification essential for function.. Proc Natl Acad Sci U S A, 107(4), 1654 - 1659. doi:10.1073/pnas.0908735107
    • Bauer CS, Rahman W, Tran-van-Minh A, Lujan R, Dickenson AH, Dolphin AC (2010). The anti-allodynic alpha(2)delta ligand pregabalin inhibits the trafficking of the calcium channel alpha(2)delta-1 subunit to presynaptic terminals in vivo.. Biochem Soc Trans, 38(2), 525 - 528. doi:10.1042/BST0380525
    • Bauer CS, Tran-Van-Minh A, Kadurin I, Dolphin AC (2010). A new look at calcium channel α2δ subunits.. Curr Opin Neurobiol, 20(5), 563 - 571. doi:10.1016/j.conb.2010.05.007
    • Dolphin AC (2010). Age of quantitative proteomics hits voltage-gated calcium channels.. Proc Natl Acad Sci U S A, 107(34), 14941 - 14942. doi:10.1073/pnas.1009102107
    • Tran-Van-Minh A, Dolphin AC (2010). The alpha2delta ligand gabapentin inhibits the Rab11-dependent recycling of the calcium channel subunit alpha2delta-2.. J Neurosci, 30(38), 12856 - 12867. doi:10.1523/JNEUROSCI.2700-10.2010
    • Kadurin I, Davies A, Laviada AA, Douglas L, Dolphin AC (2010). Association of Voltage-Gated Calcium Channel Subunit alpha(2)delta-3 with Lipid Rafts: Structural and Functional Implications. BIOPHYSICAL JOURNAL, 98(3), 693A - 693A.

    2009

    • Walsh CP, Davies A, Nieto-Rostro M, Dolphin AC, Kitmitto A (2009). Labelling of the 3D structure of the cardiac L-type voltage-gated calcium channel.. Channels (Austin), 3(6), 387 - 392.
    • Walsh CP, Davies A, Butcher AJ, Dolphin AC, Kitmitto A (2009). Three-dimensional Structure of Ca(V)3.1 COMPARISON WITH THE CARDIAC L-TYPE VOLTAGE-GATED CALCIUM CHANNEL MONOMER ARCHITECTURE. J BIOL CHEM, 284(33), 22310 - 22321. doi:10.1074/jbc.M109.017152
    • Bauer CS, Nieto-Rostro M, Rahman W, Tran-Van-Minh A, Ferron L, Douglas L, Kadurin I, Sri Ranjan Y, Fernandez-Alacid L, Millar NS, Dickenson AH, Lujan R, Dolphin AC (2009). The increased trafficking of the calcium channel subunit alpha2delta-1 to presynaptic terminals in neuropathic pain is inhibited by the alpha2delta ligand pregabalin.. J Neurosci, 29(13), 4076 - 4088. doi:10.1523/JNEUROSCI.0356-09.2009
    • Bauer C, Nieto-Rostro M, Rahman W, Tran-Van-Minh A, Ferron L, Douglas L, Kadurin I, Sri Ranjan Y, Fernandez-Alacid L, Millar NS, Dickenson AH, Lujan R, Dolphin AC (2009). The increased trafficking of the calcium channel subunit α2δ-1 to presynaptic terminals in neuropathic pain is inhibited by the α2δ ligand pregabalin.. Journal of Neuroscience, 29, 4076 - 4088.
    • Rahman W, Bauer CS, Bannister K, Vonsy J-L, Dolphin AC, Dickenson AH (2009). Descending serotonergic facilitation and the antinociceptive effects of pregabalin in a rat model of osteoarthritic pain.. Molecular Pain, 5, 45 - .
    • Dolphin AC (2009). Calcium channel diversity: multiple roles of calcium channel subunits.. Current Opinion in Neurobiology, 19, 237 - 244.
    • Walsh C, Davies A, Butcher AJ, Dolphin ACAKA (2009). 3D structure of CaV3.1 - comparison with the cardiac L-type voltage-gated calcium channel monomer architecture. Journal of Biological Chemistry, 284, 22310 - 22321.
    • Dresviannikov A, Page KM, Leroy J, Pratt WS, Dolphin AC (2009). Determinants of the voltage-dependence of G protein modulation within calcium channel ß subunits. Pflügers Archiv European Journal of Physiology, 457(4), 743 - 756. doi:10.1007/s00424-008-0549-7

    2008

    • Hendrich J, Van Minh AT, Heblich F, Nieto-Rostro M, Watschinger K, Striessnig J, Wratten J, Davies A, Dolphin AC (2008). Pharmacological disruption of calcium channel trafficking by the α2δ ligand gabapentin. Proceedings of the National Academy of Sciences of the United States of America, 105(9), 3628 - 3633. doi:10.1073/pnas.0708930105
    • Ferron L, Davies A, Page KM, Cox DJ, Leroy J, Waithe D, Butcher AJ, Sellaturay P, Bolsover S, Pratt WS, Moss FJ, Dolphin AC (2008). The stargazin-related protein gamma 7 interacts with the mRNA-binding protein heterogeneous nuclear ribonucleoprotein A2 and regulates the stability of specific mRNAs, including CaV2.2.. J Neurosci, 28(42), 10604 - 10617. doi:10.1523/JNEUROSCI.2709-08.2008
    • (2008). The stargazin-related protein γ7 interacts with mRNA binding proteins and regulates the expression and stability of specific mRNAs including CaV2.2. Journal of Neuroscience, 28(42), 10604 - 10617. doi:10.1523/JNEUROSCI.2709-08.2008
    • Heblich F, Tran-Van-Minh A, HendrichJ W, K D, A C (2008). Time course and specificity of the pharmacological disruption of the trafficking of voltage-gated calcium channels by gabapentin. Channels (Austin), 2, 4 - 9.
    • Alix JP, Dolphin AC, Fern R (2008). Vesicular apparatus, including functional calcium channels, are present in developing rodent optic nerve axons and are required for normal node of Ranvier formation. The Journal of Physiology, 586, 4069 - 4089.
    • Hendrich J, Van Minh AT, Heblich F, Nieto-Rostro M, Watschinger K, Striessnig J, Wratten J, Davies A, Dolphin AC (2008). Pharmacological disruption of calcium channel trafficking by the alpha2delta ligand gabapentin.. Proc Natl Acad Sci U S A, 105(9), 3628 - 3633. doi:10.1073/pnas.0708930105

    2007

    • Davies A, Hendrich J, Tran Van Minh A, Wratten J, Douglas L, Dolphin AC (2007). Functional biology of the α2δ subunits of voltage-gated calcium channels.. Trends in Pharmacological Sciences, 28(5), 220 - 228.
    • Davies A, Hendrich J, Van Minh AT, Wratten J, Douglas L, Dolphin AC (2007). Functional biology of the alpha(2)delta subunits of voltage-gated calcium channels.. Trends Pharmacol Sci, 28(5), 220 - 228. doi:10.1016/j.tips.2007.03.005
    • Richards MW, Leroy J, Pratt WS, Dolphin AC (2007). The HOOK domain between the SH3 and the GK domains of CaVß2 subunits contains key determinants controlling calcium channel inactivation. Channels (Austin), 1, 92 - 101.

    2006

    • Donato R, Page KM, Koch D, Nieto-Rostro M, Foucault I, Davies A, Wilkinson T, Rees M, Edwards FA, Dolphin AC (2006). The ducky(2J) mutation in Cacna2d2 results in reduced spontaneous Purkinje cell activity and altered gene expression.. J Neurosci, 26(48), 12576 - 12586. doi:10.1523/JNEUROSCI.3080-06.2006
    • Field MJ, Cox PJ, Stott E, Melrose H, Offord J, Su TZ, Bramwell S, Corradini L, England S, Winks J, Kinloch RA, Hendrich J, Dolphin AC, Webb T, Williams D (2006). Identification of the alpha(2)-delta-1 subunit of voltage-dependent calcium channels as a molecular target for pain mediating the analgesic actions of pregabalin. P NATL ACAD SCI USA, 103(46), 17537 - 17542. doi:10.1073/pnas.0409066103
    • Donato R, Page KM, Koch D, Nieto-Rostro M, Foucault I, Davies A, Wilkinson T, Rees M, Edwards FA, Dolphin AC (2006). The ducky2J mutation in Cacna2d2 results in reduced spontaneous Purkinje cell activity and altered gene expression. Journal of Neuroscience, 26(48), 12576 - 12586. doi:10.1523/JNEUROSCI.3080-06.2006
    • Douglas L, Davies A, Wratten J, Dolphin AC (2006). Do voltage-gated calcium channel alpha2delta subunits require proteolytic processing into alpha2 and delta to be functional?. Biochem Soc Trans, 34(Pt 5), 894 - 898. doi:10.1042/BST0340894
    • Dolphin AC (2006). Gender: missing the prizes that can inspire a career. NATURE, 442(7105), 868 - 868. doi:10.1038/442868a
    • Davies A, Douglas L, Hendrich J, Wratten J, Tran Van Minh A, Foucault I, Koch D, Pratt WS, Saibil HR, Dolphin AC (2006). The calcium channel alpha2delta-2 subunit partitions with CaV2.1 into lipid rafts in cerebellum: implications for localization and function.. J Neurosci, 26(34), 8748 - 8757. doi:10.1523/JNEUROSCI.2764-06.2006
    • Butcher AJ, Leroy J, Richards MW, Pratt WS, Dolphin AC (2006). The importance of occupancy rather than affinity of CaV(beta) subunits for the calcium channel I-II linker in relation to calcium channel function.. J Physiol, 574(Pt 2), 387 - 398. doi:10.1113/jphysiol.2006.109744
    • Martin SW, Butcher AJ, Berrow NS, Richards MW, Paddon RE, Turner DJ, Dolphin AC, Sihra TS, Fitzgerald EM (2006). Phosphorylation sites on calcium channel alpha1 and beta subunits regulate ERK-dependent modulation of neuronal N-type calcium channels.. Cell Calcium, 39(3), 275 - 292. doi:10.1016/j.ceca.2005.11.002
    • Dolphin AC (2006). A short history of voltage-gated calcium channels.. British Journal of Pharmacology, 147(1), S56 - S62.
    • Field MJ, Cox PJ, Stott E, Melrose H, Offord J, Su , TZ , Bramwell , S , Corradini , L , England , S , Winks , J , Kinloch , RA , Hendrich , J , Dolphin , AC , Webb , T , Williams D (2006). Identification of the α2δ-1 subunit of voltage dependent calcium channels as a novel molecular target for pain mediating the analgesic actions of pregabalin. Proceedings of the National Academy of Sciences of the United States of America, 103, 17537 - 17542.

    2005

    • Leroy J, Richards MW, Butcher AJ, Nieto-Rostro M, Pratt WS, Davies A, Dolphin AC (2005). Interaction via a key tryptophan in the I-II linker of N-type calcium channels is required for beta1 but not for palmitoylated beta2, implicating an additional binding site in the regulation of channel voltage-dependent properties.. Journal of Neuroscience, 25(30), 6984 - 6996.
    • Canti C, Nieto-Rostro M, Foucault I, Heblich F, Wratten J, Richards MW, Hendrich J, Douglas L, Page KM, Davies A, Dolphin A, C (2005). The metal-ion-dependent adhesion site in the Von Willebrand factor-A domain of α2δ subunits is key to trafficking voltage-gated Ca2+ channels.. Proceedings of the National Academy of Sciences of the United States of America, 102., 11230 - 11235. doi:10.1073/pnas.0504183102
    • Cantí C, Nieto-Rostro M, Foucault I, Heblich F, Wratten J, Richards MW, Hendrich J, Douglas L, Page KM, Davies A, Dolphin AC (2005). The metal-ion-dependent adhesion site in the Von Willebrand factor-A domain of alpha2delta subunits is key to trafficking voltage-gated Ca2+ channels.. Proc Natl Acad Sci U S A, 102(32), 11230 - 11235. doi:10.1073/pnas.0504183102
    • Leroy J, Richards MW, Butcher AJ, Nieto-Rostro M, Pratt WS, Davies A, Dolphin AC (2005). Interaction via a key tryptophan in the I-II linker of N-type calcium channels is required for beta1 but not for palmitoylated beta2, implicating an additional binding site in the regulation of channel voltage-dependent properties.. J Neurosci, 25(30), 6984 - 6996. doi:10.1523/JNEUROSCI.1137-05.2005
    • Leroy J, Richards M, Butcher AJ, Nieto-Rostro M, Pratt WS, Davies T, Dolphin AC (2005). Mutational analysis of the I-II loop of alpha(1B) reveals the importance of beta subunits for the G protein modulation of N-type calcium channels.
    • Canti C, Wratten J, Heblich F, Foucault I, Nietro-Rostro M, Douglas L, Davies T, Dolphin AC (2005). The MIDAS site in the VWF domain of alpha 2 delta-2 is essential for its stimulation of calcium channel function.

    2004

    • Wang MC, Collins RF, Ford RC, Berrow NS, Dolphin AC, Kitmitto A (2004). The three-dimensional structure of the cardiac L-type voltage-gated calcium channel: comparison with the skeletal muscle form reveals a common architectural motif. Journal of Biological Chemistry, 279, 7159 - 7168.
    • Page KM, Heblich F, Davies A, Butcher AJ, Leroy J, Bertaso F, Pratt WS, Dolphin AC (2004). Dominant-negative calcium channel suppression by truncated constructs involves a kinase implicated in the unfolded protein response.. J Neurosci, 24(23), 5400 - 5409. doi:10.1523/JNEUROSCI.0553-04.2004
    • Page KM, Heblich F, Davies A, Butcher AJ, Leroy J, Bertaso F, Pratt WS, Dolphin AC (2004). Dominant-negative calcium channel suppression by truncated constructs involves a kinase implicated in the unfolded protein response. Journal of Neuroscience, 24, 5400 - 5409.
    • Richards MW, Butcher AJ, Dolphin AC (2004). Ca2+ channel beta-subunits: structural insights AID our understanding. Trends in Pharmacological Sciences, 25, 626 - 623.
    • Viard P, Butcher AJ, Halet G, Davies A, Nürnberg B, Heblich F, Dolphin AC (2004). PI3K promotes voltage-dependent calcium channel trafficking to the plasma membrane.. Nat Neurosci, 7(9), 939 - 946. doi:10.1038/nn1300
    • Viard PV, Butcher AJ, Halet G, Davies A, Nurnberg B, Heblich F, Dolphin AC (2004). PI3K promotes voltage-dependent calcium channel trafficking to the plasma membrane. Nature Neuroscience, 7, 939 - 946. doi:10.1038/nn1300

    2003

    • Bertaso F, Ward RJ, Viard P, Milligan G, Dolphin AC (2003). Mechanism of action of Gq to inhibit G beta gamma modulation of CaV2.2 calcium channels: probed by the use of receptor-G alpha tandems. Molecular Pharmacology, 63(4), 832 - 843.
    • Moss FJ, Dolphin AC, Clare JJ (2003). Human neuronal stargazin-like proteins, gamma2, gamma3 and gamma4: an investigation of their specific localization in human brain and their influence on CaV2.1 voltage-dependent calcium channels expressed in Xenopus oocytes. BMC Neuroscience, 4(1), 23 - 40.
    • Canti C, Dolphin AC (2003). CaVbeta subunit-mediated up-regulation of CaV2.2 currents triggered by D2 dopamine receptor activation. Neuropharmacology, 45(6), 814 - 827.
    • Dolphin AC (2003). G protein modulation of voltage-gated calcium channels. Pharmacological Reviews, 55(4), 607 - 627.
    • Dolphin AC (2003). Beta subunits of voltage-gated calcium channels. Journal of Bioenergetics and Biomembranes, 35(6), 599 - 620.
    • Richards MW, Eaton JT, Butcher AJ, Berrow NS, Dolphin AC, Stephen SJ, Wallace BA (2003). Solution structure of a calcium channel beta subunit by molecular hydrodynamic methods.
    • Cox DJ, Moss FJ, Page KM, Manuela NR, Davies A, Hunt SP, Bolsover S, Dolphin AC (2003). Suppression of expression of N type calcium channels by the gamma 7 and gamma 5 stargazin-like proteins.
    • Butcher A, Heblich F, Page K, Davies A, Bertaso F, Canti C, Wratten J, Pratt WS, Dolphin AC (2003). Selectivity of inhibition of voltage-gated calcium channel expression induced by co-expression with truncated constructs.
    • Moss FJ, Dolphin AC, Clare JJ (2003). Human neuronal stargazin-like proteins, ?2, ?3 and ?4; an investigation of their specific localization in human brain and their influence on CaV2.1 voltage-dependent calcium channels expressed in Xenopus oocytes. BMC Neuroscience, 4(art.23), - . doi:10.1186/1471-2202-4-23

    2002

    • Wijetunge VS, Dolphin AC, Hughes AD (2002). Tyrosine kinases modulate voltage-operated calcium channels expressed in COS-7 cells via the alpha(1) pore-forming subunit.
    • Meir A, Dolphin AC (2002). Kinetics and Gbetagamma modulation of Ca(v)2.2 channels with different auxiliary beta subunits.. Pflugers Arch, 444(1-2), 263 - 275. doi:10.1007/s00424-002-0803-3
    • Richards MW, Hanlon MR, Berrow NS, Butcher A, Dolphin AC, Wallace BA (2002). Synchrotron radiation circular dichroism (SRCD) and CD spectroscopic studies of the voltage-dependent calcium channel beta subunit and its domains. BIOPHYS J, 82(1), 456A - 456A.
    • Brodbeck JH, Davies A, Balaguero N, Courtney JM, Canti C, Meir A, Moss FJ, Page KM, Barclay J, Hunt SPH, Gardiner MR, Rees M, Dolphin AC (2002). The ducky mouse mutation in Cacna2d2 results in altered Purkinje cell morphology and is associated with the expression of a truncated alpha(2)delta-2 protein with abnormal function. BIOPHYS J, 82(1), 572A - 572A.
    • Wang MC, Velarde G, Ford RC, Berrow NS, Dolphin AC, Kitmitto A (2002). 3D structure of the skeletal muscle dihydropyridine receptor. Journal of Molecular Biology, 232, 85 - 98.
    • Meir A, Dolphin AC (2002). Kinetics and Gáγ modulation of Cav2.2 channels with different auxiliary á subunits. Pflügers Archiv European Journal of Physiology, 21, 1514 - 1523.
    • Wijetunge S, Dolphin AC, Hughes AD (2002). Tyrosine kinases act directly on the alpha(1) subunit to modulate Ca(v)2.2 calcium channels. BIOCHEM BIOPH RES CO, 290(4), 1246 - 1249. doi:10.1006/bbrc.2001.6248
    • Brodbeck J, Davies A, Courtney JM, Meir A, Balaguero N, Canti C, Moss FJ, Page KM, Pratt WS, Hunt SP, Barclay J, Rees M, Dolphin AC (2002). The ducky mutation in Cacna2d2 results in altered Purkinje cell morphology and is associated with the expression of a truncated alpha 2 delta-2 protein with abnormal function.. J Biol Chem, 277(10), 7684 - 7693. doi:10.1074/jbc.M109404200
    • Brodbeck J, Davies A, Courtney JM, Meir A, Balaguero N, Canti C, Moss FJ, Page KM, Pratt WS, Hunt SP, Barclay J, Rees M, Dolphin AC (2002). The ducky mutation in Cacna2d2 results in altered Purkinje cell morphology and is associated with the expression of a truncated alpha 2 delta-2 protein with abnormal function. Journal of Biological Chemistry, 277(10), 7684 - 7693.
    • Moss FJ, Viard P, Davies A, Bertaso F, Page KM, Graham A, Cantí C, Plumpton M, Plumpton C, Clare JJ, Dolphin AC (2002). The novel product of a five-exon stargazin-related gene abolishes Ca(V)2.2 calcium channel expression.. EMBO J, 21(7), 1514 - 1523. doi:10.1093/emboj/21.7.1514
    • Moss FJ, Viard P, Davies A, Bertaso F, Page KM, Graham A, Canti C, Plumpton M, Plumpton C, Clare JJ, Dolphin AC (2002). The novel product of a five-exon stargazin-related gene abolishes Cav2.2 calcium channel expression. The EMBO Journal, 21(7), 1514 - 1523. doi:10.1093/emboj/21.7.1514

    2001

    • Canti C, Davies A, Berrow NS, Butcher AJ, Page KM, Dolphin AC (2001). Evidence for two concentration-dependent processes for beta subunit effects on alpha1B calcium channels. Biophysical Journal, 81, 1438 - 1451.
    • Bell DC, Butcher AJ, Berrow NS, Page KM, Brust PF, Nesterova A, Stauderman KA, Seabrook GR, Nurnberg B, Dolphin AC (2001). Biophysical properties, pharmacology and modulation of human, neuronal L-type (alpha1D, CaV1.3) voltage-dependent calcium currents. Journal of Neurophysiology, 85, 816 - 828.
    • Scholze A, Plant TD, Dolphin AC, Nurnberg B (2001). Functional expression and characterization of a voltage-gated Ca-v 1.3 (alpha(1D)) L-type Ca2+ channel subunit from an insulin-secreting cell line. N-S ARCH PHARMACOL, 363(4), R58 - R58.
    • Barclay J, Balaguero N, Mione M, Ackerman SL, Letts VA, Brodbeck J, Canti C, Meir A, Page KM, Kusumi K, Perez-Reyes E, Lander ES, Frankel WN, Gardiner RM, Dolphin AC, Rees M (2001). Ducky mouse phenotype of epilepsy and ataxia is associated with mutations in the Cacna2d2 gene and decreased calcium channel current in cerebellar Purkinje cells. Journal of Neuroscience, 21(16), 6095 - 6104.
    • Canti C, Davies A, Dolphin AC (2001). Differing concentration-dependence of calcium channel B subunit effects on expression and modulation of a1B voltage dependent calcium channels. BIOPHYS J, 80(1), 123A - 123A.
    • Raghib A, Bertaso F, Davies A, Page KM, Meir A, Bogdanov Y, Dolphin AC (2001). Dominant-negative synthesis suppression of voltage-gated calcium channel Cav2.2 induced by truncated constructs.. J Neurosci, 21(21), 8495 - 8504.
    • Raghib A, Bertaso F, Davies A, Page KM, Meir A, Bogdanov Y, Dolphin AC (2001). Dominant negative synthesis suppression of voltage-gated calcium channel CAv2.2 induced by truncated constructs. Journal of Neuroscience, 21, 8495 - 8504.
    • Scholze A, Plant TD, Dolphin AC, Nurnberg B (2001). Functional expression and characterization of a voltage-gated CAv1.3 (alpha1D) calcium channel subunit from an insulin-secreting cell line. Molecular Endocrinology, 15, 1211 - 1221.
    • Cantí C, Davies A, Berrow NS, Butcher AJ, Page KM, Dolphin AC (2001). Evidence for two concentration-dependent processes for beta-subunit effects on alpha1B calcium channels.. Biophys J, 81(3), 1439 - 1451. doi:10.1016/S0006-3495(01)75799-2

    2000

    • Meir A, Bell D, Stephens GJ, Page KM, Dolphin AC (2000). Calcium channel á subunit promotes voltage-dependent modulation of à1B by Gáγ. Biophysical Journal, 79, 731 - 746.
    • Bogdanov Y, Brice NL, Canti C, Page KM, Li M, Volsen SG, Dolphin AC (2000). Acidic motif responsible for plasma membrane association of the voltage-dependent calcium channel á1b subunit. European Journal of Neuroscience, 12, 894 - 902.
    • Burley R, Dolphin AC (2000). Overlapping selectivity of neurotoxin and dihydropyridine calcium channel blockers in cerebellar granule neurones. Neuropharmacology, 39, 1740 - 1755.
    • Canti C, Bogdanov Y, Dolphin AC (2000). Interaction between G proteins and accessory subunits in the regulation of ?1B calcium channels in Xenopus oocytes. The Journal of Physiology, 527, 419 - 432.
    • Meir A, Bell D, Stephens GJ, Page KM, Dolphin AC (2000). Calcium channel subunit promotes voltage-dependent modulation of ?1B by G. Biophysical Journal, 79, 731 - 746.
    • Meir A, Wyatt CN, Stephens GJ, Dolphin AC (2000). Neuronal T-Type Calcium Channels: Pharmacology and Investigation of Subunit Composition. In Kuba K, Higashida H, Brown DA, Yoshioka T (Ed.), Slow Synaptic Responses and Modulation (pp. 121 - 129). : Springer.
    • Stephens GJ, Page KM, Bogdanov Y, Dolphin AC (2000). The alpha1B Ca2+ channel amino terminus contributes determinants for beta subunit-mediated voltage-dependent inactivation properties. The Journal of Physiology, 525, 377 - 390.
    • Moss FJ, Plumpton M, Sanseau P, Dolphin AC, Clare JJ (2000). The identification of a family of voltage-dependent calcium channel gamma subunits: Distribution and functional effects. EUR J NEUROSCI, 12, 22 - 22.
    • Canti C, Bogdanov Y, Dolphin AC (2000). Calcium channel beta subunits bind reversibly to alpha 1b calcium channels: Concentration-dependent effect on activation and G-protein modulations.. BIOPHYS J, 78(1), 200A - 200A.
    • Balaguero N, Barclay J, Mione M, Canti C, Brodbeck J, Gardiner RM, Rees M, Dolphin AC (2000). Reduction in voltage-dependent calcium channel function in cerebellar Purkinje cells of the mouse mutant ducky, which has a null mutation for the calcium channel accessory subunit alpha 2 delta 2. J PHYSIOL-LONDON, 527, 112P - 112P.
    • Meir A, Bell DC, Stephens GJ, Page KM, Dolphin AC (2000). Calcium channel beta subunit promotes voltage-dependent modulation of alpha 1 B by G beta gamma.. Biophys J, 79(2), 731 - 746. doi:10.1016/S0006-3495(00)76331-4
    • Wijetunge S, Dolphin AC, Hughes AD (2000). Effect of tyrosine kinase inhibitors on voltage operated calcium channels expressed in COS-7 cells. J PHYSIOL-LONDON, 525, 70P - 71P.
    • Dolphin AC, Meir A, Canti C, Page KM, Bell D, Stephens GJ (2000). Mechanism of modulation of the alpha 1B voltage-gated calcium channel by G proteins. J PHYSIOL-LONDON, 523, 2S - 2S.
    • Dolphin AC, Meir A, Canti C, Page KM, Bell DC, Stephens GJ (2000). Calcium channel modulation by accessory beta subunits and G protein activation. J PHYSIOL-LONDON, 523, 90S - 91S.

    1999

    • Hanlon MR, Berrow NS, Dolphin AC, Wallace BA (1999). Modelling of a voltage-dependent Ca2+ channel á subunit as a basis for understanding its functional properties. Federation of European Biochemical Societies Letters, 445, 366 - 370.
    • Fitzgerald EM, Okuse K, Wood JN, Dolphin AC, Moss SJ (1999). cAMP-dependent phosphorylation of the tetrodotoxin-resistant voltage-dependent sodium channel SNS. The Journal of Physiology, 516(2), 433 - 446.
    • Dolphin AC, Wyatt CN, Richards J, Beattie RE, Craig P, Lee J-H, Cribbs LL, Volsen SG (1999). The effect of à2-ë and other accessory subunits on expression and properties of the calcium channel à1G. The Journal of Physiology, 519, 35 - 45.
    • Dolphin AC, Page KM, Berrow NS, Stephens GJ, Canti C (1999). Dissection of the calcium channel domains responsible for modulation of neuronal voltage-dependent calcium channels by G proteins. Annals of the New York Academy of Sciences, 868, 160 - 174.
    • Dolphin AC (1999). L-type calcium channel modulation. Advances in Second Messenger and Phosphoprotein Research, 33, 153 - 177.
    • Canti C, Page KM, Stephens GJ, Dolphin AC (1999). Identification of residues in the N-terminus of a1B critical for inhibition of the voltage-dependent calcium channel by Gáágg. Journal of Neuroscience, 19, 6855 - 6864.
    • Brice NL, Dolphin AC (1999). Differential plasma membrane targeting of voltage-dependent calcium channel subunits expressed in a polarised cell line. The Journal of Physiology, 515, 685 - 694.
    • Fitzgerald E, Okuse K, Dolphin A, Wood JN, Moss S (1999). Cyclic AMP-dependent phosphorylation of the Tetrodotoxin-resistant voltage-dependent sodium channel, SNS. The Journal of Physiology, 516, 433 - 446.

    1998

    • Dolphin AC (1998). L type calcium channel modulation by G proteins. The Journal of Physiology, , - .
    • Stephens GJ, Brice NL, Berrow NS, Dolphin AC (1998). Facilitation of alpha 1B calcium channels: Involvement of endogenous G beta gamma subunits. BIOPHYS J, 74(2), A105 - A105.
    • Stephens GJ, Cantí C, Page KM, Dolphin AC (1998). Role of domain I of neuronal Ca2+ channel alpha1 subunits in G protein modulation.. J Physiol, 509 ( Pt 1), 163 - 169.
    • Meir A, Wyatt CN, Page KM, Berrow NS, Brice NL, Dolphin AC (1998). Molecular studies on T-type voltage-dependent calcium channels. J PHYSIOL-LONDON, 509P, 5S - 6S.
    • Brice NL, Li M, Dolphin AC (1998). Subcellular targeting of voltage-dependent calcium channels in a polarized canine cell line. J PHYSIOL-LONDON, 509P, 187P - 188P.
    • Meir A, Dolphin AC (1998). Single channel properties and influence of auxiliary subunits on calcium channel alpha 1 subunits expressed in COS7 cells. J PHYSIOL-LONDON, 509P, 38P - 39P.
    • Page KM, Cantí C, Stephens GJ, Berrow NS, Dolphin AC (1998). Identification of the amino terminus of neuronal Ca2+ channel alpha1 subunits alpha1B and alpha1E as an essential determinant of G-protein modulation.. J Neurosci, 18(13), 4815 - 4824.
    • Wyatt CN, Page KM, Berrow NS, Brice NL, Dolphin AC (1998). The effect of overexpression of auxiliary Ca2+ channel subunits on native Ca2+ channel currents in undifferentiated mammalian NG108-15 cells.. J Physiol, 510 ( Pt 2), 347 - 360.
    • Dolphin AC (1998). Properties and modulation of T type currents in dorsal root ganglia and ND7-23 cells: comparison with a1E currents expressed in COS-7 cells. In Tsien RW, Clozel J-P, Nargeot J (Ed.), Low-Voltage-Activated T-type Calcium Channels (pp. 269 - 278). : Adis International.
    • Allen TJA, Mikala G, Wu X-P, Dolphin AC (1998). Effects of 2,3-butanedione monoxime (BDM) on calcium channels expressed in Xenopus oocytes. The Journal of Physiology, 508, 1 - 14.
    • Wyatt CN, Page KM, Berrow NS, Brice NL, Dolphin AC (1998). The effect of overexpression of auxiliary calcium channel subunits on native Ca2+ channel currents in undifferentiated NG108-15 cells. The Journal of Physiology, 510, 347 - 360.
    • Stephens GJ, Canti C, Page KM, Dolphin AC (1998). Role of domain I of neuronal Ca2+ channel à1 subunits in G protein modulation. The Journal of Physiology, 509, 163 - 169.
    • Stephens GJ, Brice NL, Berrow NS, Dolphin AC (1998). Facilitation of rabbit à1B calcium channels: involvement of endogenous Gáágg subunits. The Journal of Physiology, 509, 15 - 27.
    • Page KM, Canti C, Stephens GJ, Berrow NS, Dolphin AC (1998). Identification of the amino terminus of neuronal Ca2+ channel à1 subunits à1B and à1E as an essential determinant of G protein modulation. Journal of Neuroscience, 18, 4815 - 4824.
    • Meir A, Dolphin AC (1998). Known calcium channel à1 subunits can form low threshold, small conductance channels, with similarities to native T type channels. Neuron, 20, 341 - 351.
    • Dolphin AC (1998). Dissection of the calcium channel domains responsible for modulation of neuronal voltage-dependent calcium channels by G proteins. Annals of the New York Academy of Sciences, , - .
    • Dolphin AC (1998). Mechanisms of modulation of voltage-dependent calcium channels by G proteins. The Journal of Physiology, 506, 3 - 11.

    1997

    • Wyatt CN, Campbell V, Brodbeck P, Brice NL, Page KM, Berrow NS, Brickley K, Terracciano R, Naqvi RV, MacLeod KT, Dolphin AC (1997). Voltage-dependent binding and calcium current inhibition by an anti-à1D subunit antibody in rat dorsal root ganglion neurones and guinea pig myocytes. The Journal of Physiology, 502, 307 - 319.
    • Stephens GJ, Page KM, Burley JR, Berrow NS, Dolphin AC (1997). Functional expression of rat brain cloned à1E calcium channels in COS-7 cells. Pflügers Archiv European Journal of Physiology, 433, 523 - 532.
    • Page KM, Stephens GJ, Berrow NS, Dolphin AC (1997). The intracellular loop between domains I and II of the B type calcium channel confers aspects of G protein sensitivity to the E type calcium channel. Journal of Neuroscience, 17, 1330 - 1338.
    • Fitzgerald EM, Dolphin AC (1997). Regulation of rat neuronal voltage-dependent calcium channels by endogenous p21-ras. European Journal of Neuroscience, 9, 1252 - 1261.
    • Okuse K, Akopian AN, Souslova V, Sivilotti L, Dolphin AC, Wood JN (1997). Sensory neuron voltage-gated sodium channels and nociception. In Borsook D (Ed.), Molecular Neurobiology of Pain (pp. 239 - 257). : IASP Press.
    • Berrow NS, Brice NL, Tedder I, Page KM, Dolphin AC (1997). Properties of cloned rat à1A calcium channels transiently expressed in the COS-7 cell line.
    • Allen TJA, Mikala G, Dolphin AC (1997). Effects of temperature of human L-type cardiac Ca2+ channels expressed in Xenopus oocytes. Biophysical Journal, 72, - .
    • Stephens GJ, Berrow NS, Page KM, Dolphin AC (1997). The involvement of the I-II loop of the voltage-dependent calcium channel alpha(1B) in G protein modulation by G beta gamma: studies using the calcium channel beta(2) subunit that slows calcium channel inactivation.. J PHYSIOL-LONDON, 504P, P157 - P157.
    • Brice NL, Berrow NS, Campbell V, Page KM, Brickley K, Tedder I, Dolphin AC (1997). Importance of the different á subunits in the membrane expression of the à1A and à2 calcium channel subunits: studies using a depolarisation-sensitive à1A antibody.

    • Dresviannikov A, Page KM, Leroy J, Pratt WS, Dolphin AC (). Determinants of the voltage-dependence of G protein modulation within calcium channel ß subunits. Pflügers Archiv European Journal of Physiology, , - .