Prof David Brown

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Name: David Brown Email: d.a.brown@ucl.ac.uk
Title: Prof Tel: 020 7679 7297
Department: Neuroscience, Physiology & Pharmacology Fax: 020 7678 4496
Position: Principal Research Fellow Address: NPP (Pharmacology), Medawar Building, UCL South Cloisters, Gower St, London, WC1E 6BT
Research Domain: Basic Life Sciences, Experimental Medicine, Neuroscience, Personalised Medicine Web Page: Personal Web Page

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Research Description

Many neurotransmitters in the brain target receptors that couple to G proteins (G protein-coupled receptors, or GPCRs) and alter nerve cell activity indirectly by modifying the function of endogenous ion channels in the nerve cell membrane. We are interested in the mechanisms whereby GPCRs regulate ion channel activity and the consequences this has for nerve cell activity. Much of our work is directed toward studying the regulation of Kv7 potassium channels. This is a small (5-member) family of potassium channel subunits that are strikingly prone to spontaneous mutations which generate human cardiac and neural disorders. Four of these subunits (Kv7.2 to 7.5) are present in nerve cells and constitute a long-established potassium channel known as the â??M-channelâ?? (see Brown Adams, 1980: Nature, 283, 673-676). These channels are closed by activating GPCRs that couple to the Gq class of G-proteins (for example, M1 and M3 muscarinic acetylcholine receptors, or groups 1 and 5 metabotropic glutamate receptors), with a consequential increase in neural excitability. We are currently looking at four aspects of M/Kv7 channel function and regulation. (1). Channel closure through GPCR-Gq activation results from membrane phosphatidyl-4,5-bisphosphate (PIP2) hydrolysis â?" either through depletion of PIP2 (since the channels require PIP2 to open) or from effects of PIP2 hydrolysis products (release of calcium by inositol trisphosphate, and activation of protein kinase C by diacylglycerol) (see Delmas Brown, 2005: Nat.Revs. Neurosci., 2005: 6, 850-862). We are now studying the interplay between these messenger systems (see Hughes et al., 2007: Pflug.Arch., 455, 115-124). (2). Many other ion channels also require PIP2 to be fully active (see Gamper Shapiro, 2007: Nat. Revs. Neurosci., 8, 1-14). We are testing whether there is any consequent interaction between two such PIP2-dependent channels, Kv7 (M) and inward-rectifier Kv2.1 channels, and how this might arise.

Research Activities

Regulation of ion channels that control neural excitability, with special emphasis on Kv7 potassium

Education Description

UCL Collaborators

External Collaborators

Publications

    2013

    • Liu H-X, Lopatina O, Higashida C, Fujimoto H, Akther S, Inzhutova A, Liang M, Zhong J, Tsuji T, Yoshihara T, Sumi K, Ishiyama M, Ma W-J, Ozaki M, Yagitani S, Yokoyama S, Mukaida N, Sakurai T, Hori O, Yoshioka K, Hirao A, Kato Y, Ishihara K, Kato I, Okamoto H, Cherepanov SM, Salmina AB, Hirai H, Asano M, Brown DA, Nagano I, Higashida H (2013). Displays of paternal mouse pup retrieval following communicative interaction with maternal mates. NATURE COMMUNICATIONS, 4, - . doi:10.1038/ncomms2336
    • Filippov A, Brown DA (2013). A Mechanism for Nerve Cell Excitation by Norepinephrine via Alpha-1 Adrenoceptors: Inhibition of Potassium M-Current. Cellular and Molecular Neurobiology, 33(1), 1 - 4. doi:10.1007/s10571-012-9870-y
    • Brown DA (2013). The Other (Muscarinic) Acetylcholine Receptors in Sympathetic Ganglia: Actions and Mechanisms. NEUROPHYSIOLOGY, 45(1), 60 - 66. doi:10.1007/s11062-013-9337-x
    • Reilly JM, Telezhkin V, Passmore GM, Marsh SJ, Brown DA (2013). Kv7/M-type potassium channels in rat skin keratinocytes.. Pflugers Arch, 465(9), 1371 - 1381. doi:10.1007/s00424-013-1276-2
    • Telezhkin V, Thomas AM, Harmer SC, Tinker A, Brown DA (2013). A basic residue in the proximal C-terminus is necessary for efficient activation of the M-channel subunit Kv7.2 by PI(4,5)P₂.. Pflugers Arch, 465(7), 945 - 953. doi:10.1007/s00424-012-1199-3
    • Fujimoto H, Liu H-X, Lopatina O, Brown DA, Higashida H (2013). Scopolamine modulates paternal parental retrieval behavior in mice induced by the maternal mate. NEUROSCIENCE LETTERS, 546, 63 - 66. doi:10.1016/j.neulet.2013.04.059

    2012

    • Telezhkin V, Gibb AJ, Brown DA (2012). Activation of M-Type Potassium Channels by Different Membrane Phospholipids and Analogs. BIOPHYSICAL JOURNAL, 102(3), 411A - 411A.
    • Telezhkin V, Brown DA, Gibb AJ (2012). Distinct subunit contributions to the activation of M-type potassium channels by PI(4,5)P2.. J Gen Physiol, 140(1), 41 - 53. doi:10.1085/jgp.201210796
    • Passmore GM, Reilly JM, Thakur M, Keasberry VN, Marsh SJ, Dickenson AH, Brown DA (2012). Functional significance of M-type potassium channels in nociceptive cutaneous sensory endings.. Front Mol Neurosci, 5, 63 - . doi:10.3389/fnmol.2012.00063
    • Telezhkin V, Reilly JM, Thomas AM, Tinker A, Brown DA (2012). Structural requirements of membrane phospholipids for M-type potassium channel activation and binding.. J Biol Chem, 287(13), 10001 - 10012. doi:10.1074/jbc.M111.322552

    2011

    • Shah MM, Migliore M, Brown DA (2011). Differential effects of Kv7 (M-) channels on synaptic integration in distinct subcellular compartments of rat hippocampal pyramidal neurons. JOURNAL OF PHYSIOLOGY-LONDON, 589(24), 6029 - 6038. doi:10.1113/jphysiol.2011.220913

    2010

    • Brown DA, Passmore GM (2010). Some new insights into the molecular mechanisms of pain perception.. J Clin Invest, 120(5), 1380 - 1383. doi:10.1172/JCI42143
    • Filippov AK, Simon J, Barnard EA, Brown DA (2010). The scaffold protein NHERF2 determines the coupling of P2Y1 nucleotide and mGluR5 glutamate receptor to different ion channels in neurons.. J Neurosci, 30(33), 11068 - 11072. doi:10.1523/JNEUROSCI.2597-10.2010
    • Brown DA (2010). Muscarinic acetylcholine receptors (mAChRs) in the nervous system: some functions and mechanisms.. J Mol Neurosci, 41(3), 340 - 346. doi:10.1007/s12031-010-9377-2
    • Brown DA, Passmore GM (2010). Some new insights into the molecular mechanisms of pain perception.. J Clin Invest, 120, 1380 - 1383.

    2009

    2008

    • Shah MM, Migliore M, Valencia I, Cooper EC, Brown DA (2008). Functional significance of axonal Kv7 channels in hippocampal pyramidal neurons.. Proceedings of the National Academy of Sciences of the United States of America, , - .
    • Brown DA (2008). Kv7 (KCNQ) potassium channels that are mutated in human diseases. The Journal of Physiology, 586, 1781 - 1783. doi:10.1113/jphysiol.2008.153007
    • Lang PM, Fleckenstein J, Passmore GM, Brown DA, Grafe P (2008). Retigabine reduces the excitability of unmyelinated peripheral human axons. NEUROPHARMACOLOGY, 54(8), 1271 - 1278. doi:10.1016/j.neuropharm.2008.04.006
    • Lang PM, Fleckenstein J, Passmore GM, Brown DA (2008). Retigabine reduces the excitability of unmyelinated peripheral human axons.. Neuropharmacology., 54, 1271 - 1278.
    • Shah MM, Migliore M, Valencia I, Cooper EC, Brown DA (2008). Functional significance of axonal Kv7 channels in hippocampal pyramidal neurons. P NATL ACAD SCI USA, 105(22), 7869 - 7874. doi:10.1072/pnas.0802805105
    • Brown DA, Sihra TS (2008). Presynaptic signaling by heterotrimeric G-proteins.. In Südhof TC, Starke K (Ed.), Pharmacology of Neurotransmitter Release (pp. 207 - 260). : Springer. doi:10.1007/978-3-540-74805-2
    • Brown DA, Sihra TS (2008). Presynaptic signaling by heterotrimeric G-proteins.. Handb Exp Pharmacol, (184), 207 - 260. doi:10.1007/978-3-540-74805-2_8

    2007

    • Brown DA, Hughes SA, Marsh SJ, Tinker A (2007). Regulation of M(Kv7.2/7.3) channels in neurons by PIP2 and products of PIP2 hydrolysis: significance for receptor-mediated inhibition.. The Journal of Physiology, 582, 917 - 925. doi:10.1113/jphysiol.2007.132498
    • Brown DA, Lancaster B, Shah MM (2007). Hippocalcin: a new solution to an old puzzle.. Neuron, 53, 467 - 468. doi:10.1016/j.neuron.2007.01.026
    • Hughes S, Marsh SJ, Tinker A, Brown DA (2007). ) PIP(2)-dependent inhibition of M-type (Kv7.2/7.3) potassium channels: direct on-line assessment of PIP(2) depletion by Gq-coupled receptors in single living neurons.. Pflügers Archiv European Journal of Physiology, 455, 115 - 124. doi:10.1007/s00424-007-0259-6

    2006

    • Allen TG, Abogadie FC, Brown DA (2006). Simultaneous release of glutamate and acetylcholine from single magnocellular "cholinergic" basal forebrain neurons.. Journal of Neuroscience, 26(5), 1588 - 1595. doi:10.1523/JNEUROSCI.3979-05.2006
    • Filippov AK, Choi RC, Simon J, Barnard EA, Brown DA (2006). Activation of P2Y1 nucleotide receptors induces inhibition of the M-type K+ current in rat hippocampal pyramidal neurons.. J Neurosci, 26(36), 9340 - 9348. doi:10.1523/JNEUROSCI.2635-06.2006
    • Robbins J, Marsh SJ, Brown DA (2006). Probing the regulation of M (Kv7) potassium channels in intact neurons with membrane-targeted peptides.. Journal of Neuroscience, 26(30), 7950 - 7961.
    • Brown DA (2006). Acetylcholine.. British Journal of Pharmacology, 147(1), S120 - S126.
    • Street M, Marsh SJ, Stabach PR, Morrow JS, Brown DA, Buckley NJ (2006). Stimulation of Galphaq-coupled M1 muscarinic receptor causes reversible spectrin redistribution mediated by PLC, PKC and ROCK.. Journal of Cell Science, 119(8), 1528 - 1536.

    2005

    • Winks JS, Hughes S, Filippov AK, Tatulian L, Abogadie FC, Brown DA, Marsh SJ (2005). Relationship between membrane phosphatidylinositol-4,5-bisphosphate and receptor-mediated inhibition of native neuronal M channels.. Journal of Neuroscience, 25(13), 3400 - 3413. doi:10.1523/JNEUROSCI.3231-04.2005
    • Delmas P, Brown DA (2005). Pathways modulating neural KCNQ/M (Kv7) potassium channels.. Nature Reviews Neuroscience, 6(11), 850 - 862.

    2004

    • Delmas P, Nauli SM, Li X, Coste B, Osorio N, Crest M, Brown DA, Zhou J (2004). Gating of the polycystin ion channel signaling complex in neurons and kidney cells. The FASEB Journal, 18, 740 - 743.
    • Delmas P, Crest M, Brown DA (2004). Functional organization of PLC signaling microdomains in neurons. TRENDS NEUROSCI, 27(1), 41 - 47. doi:10.1016/j.tins.2003.10.1013
    • Allen TGJ, Brown DA (2004). Modulation of the excitability of cholinergic basal forebrain neurones by KATP channels. The Journal of Physiology, 554(2), 353 - 370. doi:10.1113/jphysiol.2003.055889
    • Delmas P, Crest M, Brown DA (2004). Functional organization of PLC signaling microdomains in neurons. Trends in Neurosciences, 27(1), 41 - 47. doi:10.1016/j.tins.2003.10.013
    • Filippov AK, Fernandez-Fernandez JM, Marsh SJ, Simon J, Barnard EA, Brown DA (2004). Activation and inhibition of neuronal G protein-gated inwardly rectifying K(+) channels by P2Y nucleotide receptors. Molecular Pharmacology, 66, 468 - 477.

    2003

    • Barnard EA, Simon J, Tsim KWK, Filippov AK, Brown DA (2003). Signalling pathways and ion channel regulations of P2Y receptors. doi:10.1002/ddr.10200
    • Brown DA (2003). Functional characterization of KCNQ channels and diversity of M channels.
    • Wanaverbecq N, Marsh SJ, Al-Qatari M, Brown DA (2003). The plasma membrane calcium-ATPase as a major mechanism for intracalcium regulation in neurones from the rat superior cervical ganglion. The Journal of Physiology, 550, 83 - 101.
    • Tatulian L, Brown DA (2003). Effect of the KCNQ potassium channel opener retigabine on single KCNQ channels expressed in CHO Cells. The Journal of Physiology, 549, 57 - 63.
    • Filippov AK, Simon J, Barnard EA, Brown DA (2003). Coupling of the nucleotide P2Y4 receptor to neuronal ion channels. British Journal of Pharmacology, 138(2), 400 - 406.
    • Hoshi N, Zhang J-S, Omaki M, Takeuchi T, Yokoyama S, Wanaverbecq N, Langeberg LK, Yoneda Y, Scott JD, Brown DA, Higashida H (2003). AKAP150 signaling promotes suppression of the M-current by muscarinic agonists.. Nature Neuroscience, 6(6), 564 - 571.
    • Hadley JK, Passmore GM, Tatulian L, Al-Qatari M, Ye F, Wickenden AD, Brown DA (2003). Stoichiometry of expressed KCNQ2/KCNQ3 potassium channels and subcomposition of native ganglionic M channels deduced from block by tetraethylammonium. Journal of Neuroscience, 23(12), 5012 - 5019.
    • Passmore GM, Selyanko AA, Mistry M, Al Qatari M, Marsh SJ, Matthews EA, Dickenson AH, Brown TA, Burbidge SA, Main M, Brown DA (2003). KCNQ/M currents in sensory neurons: significance for pain therapy. Journal of Neuroscience, 23(18), 7227 - 7236.

    2002

    • Abogadie FC, Bron R, Marsh SJ, Drew LJ, Haley JE, Buckley NJ, Brown DA, Delmas P (2002). Adenovirus-mediated G?q-protein antisense transfer in neuronsreplicates G?q gene knockout strategies. Neuropharmacology, 42(7), 950 - 957.
    • Brown DA (2002). G-proteins. In Foreman JC, Johansen T (Ed.), Textbook of Receptor Pharmacology (pp. 213 - 236). : CRC Press.
    • Delmas P, Brown DA (2002). Junctional signaling microdomains: bridging the gap between the neuronal cell surface and Ca2+ stores. Neuron, 36(5), 787 - 790.
    • Simon J, Filippov AK, Goransson S, Wong YH, Frelin C, Michel AD, Brown DA, Barnard EA (2002). Characterization and channel coupling of the P2Y12 nucleotide receptor of brain capillary endothelial cells. Journal of Biological Chemistry, 277(35), 31390 - 31400.
    • Brown DA, Selyanko AA, Hadley JK, Tatulian L (2002). Some pharmacological properties of neural KCNQchannels. Neurophysiology, 34(2/3), 91 - 94.
    • Abogadie FC, Bron R, Marsh SJ, Drew LJ, Haley JE, Buckley NJ, Brown DA, Delmas P (2002). Adenovirus-mediated G(alpha)(q)-protein antisense transfer in neurons replicates G(alpha)(q) gene knockout strategies.. Neuropharmacology, 42(7), 950 - 957.
    • Selyanko AA, Delmas P, Hadley JK, Tatulian L, Wood IC, Mistry M, London B, Brown DA (2002). Dominant-negative subunits reveal potassium channel families that contribute to M-like potassium currents.. J Neurosci, 22(5), RC212 - .
    • Shah MM, Mistry M, Marsh SJ, Brown DA, Delmas P (2002). Molecular correlates of the M-current in cultured rat hippocampal neurons. Journal of Physiology, 544(1), 29 - 37. doi:10.1113/jphysiol.2002.028571
    • Delmas P, Wanaverbecq N, Abogadie FC, Mistry M, Brown DA (2002). Signaling microdomains define the specificity of receptor-medicated InsP3 pathways in neurons. Neuron, 34, 209 - 220. doi:10.1016/S0896-6273(02)01097-8
    • Selyanko AA, Delmas P, Hadley JK, Tatulian L, Wood IC, Mistry M, London B, Brown DA (2002). Dominant-negative subunits reveal potassium channel families that contribute to M-like potassium currents. Journal of Neuroscience, 22(0), 1 - 5.
    • Delmas P, Nomura H, Li X, Lakkis M, Luo Y, Segal Y, Fernández-Fernández JM, Harris P, Frischauf A, Brown DA, Zhou J (2002). Constitutive Activation of G-proteins by Polycystin-1 Is Antagonized by Polycystin-2. Journal of Biological Chemistry, 277(13), 11276 - 11283. doi:10.1074/jbc.M110483200

    2001

    • Brown DA (2001). Control of Neuronal Activity. In Webster RA (Ed.), Neurotransmitters, Drugs and Brain Function (pp. 33 - 56). : Wiley.
    • Tatulian L, Delmas P, Abogadie FC, Brown DA (2001). Activation of expressed KCNQ potassium currents and native neuronal M-type currents by the anti-convulsant drug retigabine. Journal of Neuroscience, 21, 5535 - 5545.
    • Pan Z, Selyanko AA, Hadley JK, Brown DA, Dixon JE, McKinnon D (2001). Alternative splicing of KCNQ2 potassium channel transcripts contributes to the functional diversity of M-currents. The Journal of Physiology, 531, 347 - 358.
    • Korolkova YV, Kozlov SA, Lipkin AV, Pluzhnikov KA, Hadley JK, Filippov AK, Brown DA, Angelo K, Strobaek D, Jespersen T, Olesen SP, Jensen BS, Grishin EV (2001). An ERG channel inhibitor from the scorpion Buthus eupeus. Journal of Biological Chemistry, 276, 9868 - 9876.
    • Robbins MJ, Calver AR, Filippov AK, Hirst W, Russell RB, Wood M, Nasir S, Couve A, Brown DA, Moss SJ, Pangalos M (2001). GABAB2 is essential for G-protein coupling of the GABAB receptor heterodimer. Journal of Neuroscience, 21, 8043 - 8052.
    • Fernandez-Fernandez JM, Abogadie FC, Milligan G, Delmas P, Brown DA (2001). Multiple pertussis toxin-sensitive G proteins can couple receptors to GIRK channels in rat sympathetic neurones when heterologously-expressed, but only native G1 proteins do so in situ. European Journal of Neuroscience, 14, 283 - 292.
    • Selyanko AA, Hadley JK, Brown DA (2001). Properties of single M-type KCNQ2/KCNQ3 potassium channels expressed in mammalian cells. The Journal of Physiology, 534, 15 - 24.

    2000

    • Haley JE, Delmas P, Offermans S, Abogadie FC, Simon MI, Buckley NJ, Brown DA (2000). Muscarinic inhibition of calcium current and M current in G?q-difficient mice. Journal of Neuroscience, 20, 3973 - 3979.
    • Kittler JT, Delmas P, Jovanovic JN, Brown DA, Smart TG, Moss SJ (2000). Constitutive endocytosis of GABA(A) receptors by an association with the adaptin AP2 complex modulates inhibitory synaptic currents in hippocampal neurons. Journal of Neuroscience, 20(21), 7972 - 7977.
    • Delmas P, Abogadie FC, Buckley NJ, Brown DA (2000). Calcium channel gating and modulation by neurotransmitters depend on cellular compartmentalization. Nature Neuroscience, 3, 670 - 678.
    • Brown DA (2000). Neurobiology: The acid text for resting potassium channels. Current Biology, 10, - .
    • Brown DA (2000). M-Currents: From Discovery to Single Channel Currents. In Kuba K, Higashida H, Brown DA, Yoshioka T (Ed.), Slow Synaptic Responses and Modulation (pp. 15 - 26). : Springer.
    • Brandon N, Delmas P, Kittler JT, McDonald BJ, Sieghart W, Brown DA, Smart TG, Moss SJ (2000). GABAA receptor phosphorylation and functional modulation in cortical neurons by a protein kinase C-dependent pathway. Journal of Biological Chemistry, 275, 38856 - 38862.
    • Marsh SJ, Wanaverbecq N, Selyanko AA, Brown DA (2000). Calcium Signalling in Neurons Exemplified by Rat Sympathetic Ganglion Cells. In Pochet R, Donato R, Haiech J, Heizmann C, Gerke V (Ed.), Calcium: The Molecular Basis of Calcium Action in Biology and Medicine (pp. 27 - 44). : Kluwer Academic Publishers.
    • Brown DA, Filippov AK, Barnard EA (2000). Inhibition of potassium and calcium currents in neurones bymolecularly-defined P2Y receptors. Journal of the Autonomic Nervous System, 81, 31 - 36.
    • Selyanko AA, Hadley JK, Wood IC, Abogadie FC, Jentsch TJ, Brown DA (2000). Inhibition of KCNQ1-4 channels expressed in mammalian cells via M1 muscarinic acetylcholine receptors. The Journal of Physiology, 522, 349 - 355.
    • Hadley JK, Noda M, Selyanko AA, Wood IC, Abogadie FC, Brown DA (2000). Differential tetraethylammonium sensitivity of KCNQ1-4 potassium channels. British Journal of Pharmacology, 129, 413 - 415.
    • Brown DA (2000). The History of the Neuronal Nicotinic Receptors. In Clementi F, Fornasari D, Gotti C (Ed.), Handbook of Experimental Pharmacology, vol. 144 (pp. 3 - 11). : Springer-Verlag.
    • Higashida H, Brown DA, Robbins J (2000). Both linopirdine- and WAY123,398-sensitive components of I-K(M,I-ng) are modulated by cyclic ADP ribose in NG108-15 cells. PFLUG ARCH EUR J PHY, 441(2-3), 228 - 234.
    • Buckley NJ, Abogadie FC, Brown DA, Dayrell M, Caulfield MP, Delmas P, Haley JE (2000). Use of antisense expression plasmids to attenuate G-protein expression in primary neurons. METHOD ENZYMOL, 314, 136 - 148.
    • Haley JE, Delmas P, Offermanns S, Abogadie FC, Simon MI, Buckley NJ, Brown DA (2000). Muscarinic inhibition of calcium current and M current in Galpha q-deficient mice.. J Neurosci, 20(11), 3973 - 3979.
    • Haley JE, Abogadie FC, Fernandez-Fernandez JM, Dayrell M, Vallis Y, Buckley NJ, Brown DA (2000). Bradykinin, but not muscarinic, inhibition of M-current in rat sympathetic ganglion neurons involves phospholipase C-á4. Journal of Neuroscience, 20, - .
    • Brown DA (2000). Introductory Review: K Currents and Modulation. In Kuba K, Higashida H, Brown DA, Yoshioka T (Ed.), Slow Synaptic Responses and Modulation (pp. 52 - 58). : Springer.
    • Filippov AK, Brown DA, Barnard EA (2000). The P2Y(1) receptor closes the N-type Ca(2+) channel in neurones, with both adenosine triphosphates and diphosphates as potent agonists.. Br J Pharmacol, 129(6), 1063 - 1066. doi:10.1038/sj.bjp.0703185
    • Filippov AK, Brown DA, Barnard EA (2000). The P2Y1 receptor closes the N-type Ca2+ channel in neurones, with both adenosine triphosphates and diphosphates as potent agonists. British Journal of Pharmacology, 129, 1063 - 1066.
    • Filippov AK, Couve A, Pangalos MN, Walsh FS, Brown DA, Moss SJ (2000). Heteromeric assembly of GABABR1 and GABABR2 receptor subunits inhibits Ca2+ current in sympathetic neurons. Journal of Neuroscience, 20, 2867 - 2874.
    • Filippov AK, Couve A, Pangalos MN, Walsh FS, Brown DA, Moss SJ (2000). Heteromeric assembly of GABA(B)R1 and GABA(B)R2 receptor subunits inhibits Ca(2+) current in sympathetic neurons.. J Neurosci, 20(8), 2867 - 2874.
    • Selyanko AA, Hadley JK, Wood IC, Abogadie FC, Jentsch TJ, Brown DA (2000). Inhibition of KCNQ1-4 potassium channels expressed in mammalian cells via M1 muscarinic acetylcholine receptors.. J Physiol, 522 Pt 3, 349 - 355.

    1999

    • Selyanko AA, Hadley JK, Wood IC, Abogadie FC, Delmas P, Buckley NJ, London B, Brown DA (1999). Two types of K+ channel subunit, Erg1 and KCNQ2/3, contribute to the M-like current in a mammalian neuronal cell. Journal of Neuroscience, 19, 7742 - 7756.
    • Delmas P, Abogadie FC, Milligan G, Buckley NJ, Brown DA (1999). ?? dimers derived from Go and Gi proteins contribute differentcomponents of adrenergic inhibition of Ca2+ channels in ratsympathetic neurones. The Journal of Physiology, 518(1), 23 - 36. doi:10.1111/j.1469-7793.1999.0023r.x
    • Delmas P, Abogadie FC, Milligan G, Buckley NJ, Brown DA (1999). áágg dimers derived from both G0 and G1G protein heterotrimers contribute different components of à2-adrenoceptor mediated inhibition of Ca2+ channels in rat sympathetic neurones. The Journal of Physiology, 518, 23 - 36.
    • Filippov AK, Webb TE, Barnard EA, Brown DA (1999). Dual coupling of heterologously-expressed rat P2Y6 nucleotide receptors to N-type Ca2+ and M-type K+ currents in rat sympathetic neurones. British Journal of Pharmacology, 126, 1009 - 1017.
    • Selyanko AA, Brown DA (1999). M-channel gating and simulation. Biophysical Journal, 77, 701 - 713.
    • Fernandez-Fernandez JM, Wanaverbecq N, Halley P, Caulfield MP, Brown DA (1999). A role for M2 receptors in the muscarinic activation of G protein-gated K+ (GIRK) channels expressed in isolated rat sympathetic neurones. The Journal of Physiology, 515, 631 - 637.
    • Fernandez-Fernandez JM, Wanaverbecq N, Halley P, Caulfield MP, Brown DA (1999). Selective activation of heterologously expressed G protein-gated K+ channels by M2 muscarinic receptors in rat sympathetic neurones.. J Physiol, 515 ( Pt 3), 631 - 637.
    • Filippov AK, Webb TE, Barnard EA, Brown DA (1999). Dual coupling of heterologously-expressed rat P2Y6 nucleotide receptors to N-type Ca2+ and M-type K+ currents in rat sympathetic neurones.. Br J Pharmacol, 126(4), 1009 - 1017. doi:10.1038/sj.bjp.0702356
    • Selyanko AA, Hadley JK, Wood IC, Abogadie FC, Delmas P, Buckley NJ, London B, Brown DA (1999). Two types of K(+) channel subunit, Erg1 and KCNQ2/3, contribute to the M-like current in a mammalian neuronal cell.. J Neurosci, 19(18), 7742 - 7756.

    1998

    • Hadley JK, Selyanko AA, Brown DA (1998). Activation profiles and modulation of rodent Kv1.2 channels expressed in mammalian cell lines.
    • Filippov AK, Webb TE, Barnard EA, Brown DA (1998). P2Y2 nucleotide receptors expressed heterologously in sympathetic neurons inhibit both N-type Ca2+ and M-type K+ currents. Journal of Neuroscience, 18, 5170 - 5179.
    • Selyanko AA, Menendez de la Prida L, Marsh SJ, Brown DA (1998). Control of resting submembrane Ca2+ in rat sympathetic neurones studied using K+ channels as Ca2+ detectors.
    • Selyanko AA, Sim JA, Brown DA (1998). Small Ca2+-activated K+ (SKCa) channels in cultured rat hippocampal pyramidal neurones. Pflügers Archiv European Journal of Physiology, 437, 161 - 163.
    • Delmas P, Brown DA, Dayrell M, Abogadie FC, Caulfield MP, Buckley NJ (1998). On the role of endogenous G-protein áágg subunits in ICa inhibition by neurotransmitters in rat sympathetic neurones. The Journal of Physiology, 506, 319 - 329.
    • Delmas P, Abogadie FC, Dayrell M, Haley JE, Milligan G, Caulfield MP, Brown DA, Buckley NJ (1998). G-proteins and G-protein subunits mediating cholinergic inhibition of N-type calcium currents in sympathetic neurons. European Journal of Neuroscience, 10(5), 1654 - 1666.
    • Haley JE, Abogadie FC, Delmas P, Dayrell M, Vallis Y, Milligan G, Caulfield MP, Brown DA, Buckley NJ (1998). The à subunit of Gq contributes to muscarinic inhibition of the M-type potassium current in sympathetic neurons. Journal of Neuroscience, 18, 4521 - 4531.
    • Couve A, Filippov AK, Connolly CN, Bettler B, Brown DA, Moss SJ (1998). Intracellular retention of recombinant GABAB receptors.. J Biol Chem, 273(41), 26361 - 26367.
    • Selyanko AA, Sim JA, Brown DA (1998). Small (SKCa) Ca2+-activated K+ channels in cultured rat hippocampal pyramidal neurones.. Pflugers Arch, 437(1), 161 - 163.
    • Brown DA, Abogadie FC, Allen TGJ, Buckley NJ, Delmas P, Dayrell M, Haley JE (1998). Some functional effects of postsynaptic and presynaptic muscarinic receptors in the nervous system.
    • Delmas P, Brown DA, Dayrell M, Abogadie FC, Caulfield MP, Buckley NJ (1998). On the role of endogenous G-protein ?? subunits in N-typeCa2+ current inhibition by neurotransmitters in ratsympathetic neurones. The Journal of Physiology, 506(2), 319 - 329. doi:10.1111/j.1469-7793.1998.319bw.x
    • Couve A, Filippov AK, Bettler B, Brown DA, Moss SJ (1998). Intracellular retention of recombinant GABAB receptors. Journal of Biological Chemistry, 273, 26361 - 26367.
    • Filippov AK, Webb TE, Barnard EA, Brown DA (1998). P2Y2 nucleotide receptors expressed heterologously in sympathetic neurons inhibit both N-type Ca2+ and M-type K+ currents.. J Neurosci, 18(14), 5170 - 5179.

    1997

    • Filippov AK, Webb TE, Barnard EA, Brown DA (1997). Inhibition of neuronal calcium currents by heterologously-expressed P2Y2 purnioceptors.
    • Brown DA, Abogadie FC, Allan TGJ, Buckley NJ, Caulfield MP, Delmas P, Haley JE, Lamas JA, Selyanko AA (1997). Muscarinic mechanisms in nerve cells. Life Sciences, 60, 1137 - 1144.
    • Brown DA, Selyanko AA, Haley JE, Delmas P, Abogadie FC, Buckley NJ (1997). Regulation of potassium channels: M-type channels.
    • Delmas P, Abogadie FC, Caulfield MP, Buckley NJ, Brown DA (1997). Differential G protein subunit basis for biophysically distinct inhibitions of ICa by muscarinic receptors in sympathetic neurones.
    • Leaney JL, Marsh SJ, Brown DA (1997). A neuronal swelling-activated chloride current and its role in volume regulation.
    • Stansfeld CE, Ludwig J, Roeper J, Weseloh R, Pongs O, Brown DA (1997). Does r-EAG contribute to the M-current?. Trends in Neurosciences, 20, 243 - 244.
    • Filippov AK, Webb TE, Barnard EA, Brown DA (1997). Inhibition by heterologously-expressed P2Y2 nucleotide receptors on N-type calcium currents in rat sympathetic neurones. British Journal of Pharmacology, 121, 849 - 852.
    • Haley JE, Delmas P, Abogadie FC, Dayrell M, Caulfield MP, Buckley NJ, Brown DA (1997). Muscarinic inhibition of the M-current is mediated by the à subunit of Gq.
    • Stansfeld CE, Ludwig J, Roeper J, Weseloh R, Brown DA, Pongs O (1997). A Physiological role for the ether-a-go-go K+ channels?. Trends in Neurosciences, 20, 13 - 14.
    • Lamas JA, Selyanko AA, Brown DA (1997). Effects of a cognition-enhancer, linopirdine (DuP 996), on M-type potassium currents (KK(M) and some other voltage- and ligand-gated membrane currents in rat sympathetic neurons. European Journal of Neuroscience, 9, 605 - 616.
    • Brown DA (1997). The regulation of some ion channels involved in controlling neuronal excitability.
    • Brown DA, Marsh SJ, Selyanko AA (1997). Regulation of ion channels in sympathetic neurons by intracellular calcium.
    • Selyanko AA, Brown DA (1997). Gating kinetics and simulation of potassium M-channels in rat sympathetic neurons.
    • Leaney JL, Marsh SJ, Brown DA (1997). A swelliing-activated chloride current in rat sympathetic neurones. The Journal of Physiology, 501, 555 - 564.

    1996

    • Selyanko AA, Brown DA (1996). Regulation of M-type potassium channels in mammalian sympathetic neurons: action of intracellular calcium on single channel currents.. Neuropharmacology, 35(7), 933 - 947.
    • Stansfeld CE, Roper J, Ludwig J, Weseloh RM, Marsh SJ, Brown DA, Pongs O (1996). Elevation of intracellular calcium by muscarinic receptor activation induces a block of voltage-activated rat ether-a-go-go channels in a stably transfected cell line. P NATL ACAD SCI USA, 93(18), 9910 - 9914.
    • Selyanko AA, Brown DA (1996). Intracellular calcium directly inhibits potassium M channels in excised membrane patches from rat sympathetic neurons.. Neuron, 16(1), 151 - 162.
    • Filippov AK, Kozlov SA, Pluzhnikov KA, Grishin EV, Brown DA (1996). M-type K+ current inhibition by a toxin fron the scorpion Buthus eupeus.. FEBS Lett, 384(3), 277 - 280.
    • Filippov AK, Brown DA (1996). Activation of nucleotide receptors inhibits high-threshold calcium currents in NG108-15 neuronal hybrid cells.. Eur J Neurosci, 8(6), 1149 - 1155.
    • Allen TG, Brown DA (1996). Detection and modulation of acetylcholine release from neurites of rat basal forebrain cells in culture.. J Physiol, 492 ( Pt 2), 453 - 466.

    1995

    • ISHIZAKA N, NODA M, KIMURA Y, HASHII M, FUKUDA K, KATAYAMA M, BROWN DA, HIGASHIDA H (1995). INOSITOL 1,4,5-TRISPHOSPHATE FORMATION AND RYANODINE-SENSITIVE OSCILLATIONS OF CYTOSOLIC-FREE CA2+ CONCENTRATIONS IN NEUROBLASTOMA X FIBROBLAST HYBRID NL308 CELLS EXPRESSING M2 AND M4 MUSCARINIC ACETYLCHOLINE-RECEPTOR SUBTYPES. PFLUG ARCH EUR J PHY, 429(3), 426 - 433.
    • Selyanko AA, Robbins J, Brown DA (1995). Putative M-type potassium channels in neuroblastoma-glioma hybrid cells: inhibition by muscarine and bradykinin.. Receptors Channels, 3(2), 147 - 159.
    • Jones S, Brown DA, Milligan G, Willer E, Buckley NJ, Caulfield MP (1995). Bradykinin excites rat sympathetic neurons by inhibition of M current through a mechanism involving B2 receptors and G alpha q/11.. Neuron, 14(2), 399 - 405.
    • Marsh SJ, Trouslard J, Leaney JL, Brown DA (1995). Synergistic regulation of a neuronal chloride current by intracellular calcium and muscarinic receptor activation: a role for protein kinase C.. Neuron, 15(3), 729 - 737.

    1994

    • Alger BE, Sim JA, Brown DA (1994). Single-channel activity correlated with medium-duration, Ca-dependent K current in cultured rat hippocampal neurones.. Neurosci Lett, 168(1-2), 23 - 28.
    • Caulfield MP, Jones S, Vallis Y, Buckley NJ, Kim GD, Milligan G, Brown DA (1994). Muscarinic M-current inhibition via G alpha q/11 and alpha-adrenoceptor inhibition of Ca2+ current via G alpha o in rat sympathetic neurones.. J Physiol, 477 ( Pt 3), 415 - 422.
    • Filippov AK, Selyanko AA, Robbins J, Brown DA (1994). Activation of nucleotide receptors inhibits M-type K current [IK(M)] in neuroblastoma x glioma hybrid cells.. Pflugers Arch, 429(2), 223 - 230.

    1993

    • Selyanko AA, Brown DA (1993). Effects of membrane potential and muscarine on potassium M-channel kinetics in rat sympathetic neurones.. J Physiol, 472, 711 - 724.
    • Trouslard J, Marsh SJ, Brown DA (1993). Calcium entry through nicotinic receptor channels and calcium channels in cultured rat superior cervical ganglion cells.. J Physiol, 468, 53 - 71.
    • Robbins J, Marsh SJ, Brown DA (1993). On the mechanism of M-current inhibition by muscarinic m1 receptors in DNA-transfected rodent neuroblastoma x glioma cells.. J Physiol, 469, 153 - 178.
    • Allen TG, Brown DA (1993). M2 muscarinic receptor-mediated inhibition of the Ca2+ current in rat magnocellular cholinergic basal forebrain neurones.. J Physiol, 466, 173 - 189.
    • Brown DA, Higashida H, Noda M, Ishizaka N, Hashii M, Hoshi N, Yokoyama S, Fukuda K, Katayama M, Nukada T (1993). Coupling of muscarinic receptor subtypes to ion channels: experiments on neuroblastoma hybrid cells.. Ann N Y Acad Sci, 707, 237 - 258.
    • Stansfeld CE, Marsh SJ, Gibb AJ, Brown DA (1993). Identification of M-channels in outside-out patches excised from sympathetic ganglion cells.. Neuron, 10(4), 639 - 654.
    • Caulfield MP, Robbins J, Higashida H, Brown DA (1993). Postsynaptic actions of acetylcholine: the coupling of muscarinic receptor subtypes to neuronal ion channels.. Prog Brain Res, 98, 293 - 301.
    • Allen TG, Sim JA, Brown DA (1993). The whole-cell calcium current in acutely dissociated magnocellular cholinergic basal forebrain neurones of the rat.. J Physiol, 460, 91 - 116.
    • Cloues R, Jones S, Brown DA (1993). Zn2+ potentiates ATP-activated currents in rat sympathetic neurons.. Pflugers Arch, 424(2), 152 - 158.

    1992

    • Sim JA, Gerber U, Knöpfel T, Brown DA (1992). Evidence Against a Role for Protein Kinase C in the Inhibition of the Calcium-activated Potassium Current IAHP by Muscarinic Stimulants in Rat Hippocampal Neurons.. Eur J Neurosci, 4(9), 785 - 791.
    • Robbins J, McFadzean I, Brown DA (1992). Effects of bradykinin on ion conductances in NG108-15 neuroblastoma x glioma hybrid cells recorded with patch-clamp electrodes.. Agents Actions Suppl, 38 ( Pt 2), 98 - 107.
    • Caulfield MP, Brown DA (1992). Cannabinoid receptor agonists inhibit Ca current in NG108-15 neuroblastoma cells via a pertussis toxin-sensitive mechanism.. Br J Pharmacol, 106(2), 231 - 232.
    • Jones S, Robbins J, Brown DA (1992). Neurotransmitter modulation of calcium channels is dependent on the charge carrier used in the recording of currents.. Neurosci Lett, 145(2), 153 - 156.
    • Selyanko AA, Stansfeld CE, Brown DA (1992). Closure of potassium M-channels by muscarinic acetylcholine-receptor stimulants requires a diffusible messenger.. Proc Biol Sci, 250(1328), 119 - 125. doi:10.1098/rspb.1992.0139
    • Robbins J, Trouslard J, Marsh SJ, Brown DA (1992). Kinetic and pharmacological properties of the M-current in rodent neuroblastoma x glioma hybrid cells.. J Physiol, 451, 159 - 185.
    • Robbins J, Cloues R, Brown DA (1992). Intracellular Mg2+ inhibits the IP3-activated IK(Ca) in NG108-15 cells. [Why intracellular citrate can be useful for recording IK(Ca)].. Pflugers Arch, 420(3-4), 347 - 353.
    • Caulfield MP, Robbins J, Brown DA (1992). Neurotransmitters inhibit the omega-conotoxin-sensitive component of Ca current in neuroblastoma x glioma hybrid (NG 108-15) cells, not the nifedipine-sensitive component.. Pflugers Arch, 420(5-6), 486 - 492.

    1991

    • Caulfield MP, Brown DA (1991). Pharmacology of the putative M4 muscarinic receptor mediating Ca-current inhibition in neuroblastoma x glioma hybrid (NG 108-15) cells.. Br J Pharmacol, 104(1), 39 - 44.
    • Marsh SJ, Brown DA (1991). Potassium currents contributing to action potential repolarization in dissociated cultured rat superior cervical sympathetic neurones.. Neurosci Lett, 133(2), 298 - 302.
    • Robbins J, Caulfield MP, Higashida H, Brown DA (1991). Genotypic m3-Muscarinic Receptors Preferentially Inhibit M-currents in DNA-transfected NG108-15 Neuroblastoma x Glioma Hybrid Cells.. Eur J Neurosci, 3(8), 820 - 824.
    • Caulfield MP, Robbins J, Sim JA, Brown DA, Mac Neil S, Blackburn GM (1991). The naphthalenesulphonamide calmodulin antagonist W7 and its 5-iodo-1-C8 analogue inhibit potassium and calcium currents in NG108-15 neuroblastoma x glioma cells in a manner possibly unrelated to their antagonism of calmodulin.. Neurosci Lett, 125(1), 57 - 61.

    1990

    • Marsh SJ, Hubbard A, Brown DA (1990). Some Actions of 9-Amino-1,2,3,4-Tetrahydroacridine (THA) on Cholinergic Transmission and Membrane Currents in Rat Sympathetic Ganglia.. Eur J Neurosci, 2(12), 1127 - 1134.
    • Brown DA, Gähwiler BH, Griffith WH, Halliwell JV (1990). Membrane currents in hippocampal neurons.. Prog Brain Res, 83, 141 - 160.
    • Brown DA (1990). Regulation of cell excitability.. Curr Opin Cell Biol, 2(2), 221 - 230.
    • Owen DG, Marsh SJ, Brown DA (1990). M-current noise and putative M-channels in cultured rat sympathetic ganglion cells.. J Physiol, 431, 269 - 290.
    • Brown DA (1990). G-proteins and potassium currents in neurons.. Annu Rev Physiol, 52, 215 - 242. doi:10.1146/annurev.ph.52.030190.001243

    1989

    • Brown DA, Docherty RJ, McFadzean I (1989). Calcium channels in vertebrate neurons. Experiments on a neuroblastoma hybrid model.. Ann N Y Acad Sci, 560, 358 - 372.
    • McFadzean I, Mullaney I, Brown DA, Milligan G (1989). Antibodies to the GTP binding protein, Go, antagonize noradrenaline-induced calcium current inhibition in NG108-15 hybrid cells.. Neuron, 3(2), 177 - 182.

    1988

    • Brown DA, Higashida H, Adams PR, Marrion NV, Smart TG (1988). Role of G-protein-coupled phosphatidylinositide system in signal transduction in vertebrate neurons: experiments on neuroblastoma hybrid cells and ganglion cells.. Cold Spring Harb Symp Quant Biol, 53 Pt 1, 375 - 384.

    1985

    • BROWN DA, ADAMS PR, JONES SW, GRIFFITH WH, HILLS J, CONSTANTI A (1985). SOME IONIC MECHANISMS OF SLOW PEPTIDERGIC NEURONAL EXCITATION. REGUL PEPTIDES, , 157 - 164.

    • BROWN D (). Muscarinic acetylcholine receptors (mAChRs) in the nervous system: some functions and mechanisms.. J Mol Neurosci, 41, 330 - 336.