Centre for Nephrology
Nephrology is part of the Division of Medicine

Medicine News

Lilly Scientific Achievement Award 2014 to Rachel Batterham

Published: Nov 20, 2014 9:40:05 AM

Sheila Sherlock Updates 4-6 December 2014

Published: Nov 17, 2014 11:14:07 AM

The good, the bad and the ugly: Bugs in the gut

Published: Nov 17, 2014 8:18:03 AM

Techniques

PHYSIOLOGY

Principal Investigator: Professor Robert Unwin, Dr Joanne Marks, Dr Anselm Zdebik

Renal micropuncture techniques
In vivo and isolated gut loop (transport) techniques
Brush border membrane vesicle uptake technique
Electrophysiology: twin electrode voltage clamp (TEVC), ion-selective microelectrodes, TEVC combined with pH in oocytes, calcium and pH imaging in mammalian cells
Extracellular recording of neuronal activity
2-photon (calcium) imaging
Expression profiling, surface expression, proteomics

LIPID GROUP

Principal Investigator: Dr Xiong-Zhong Ruan

Lipid biochemistry, cellular cholesterol and fatty acid assay, LDL purification
Molecular biology
Cell biology: renal and vascular cell culture
Molecular imaging: confocal microscopy
Animal models of dyslipidaemia and inflammation

PROTEOMICS & MOLECULAR CELL DYNAMICS GROUP

Principal Investigator: Professor Jasminka Godovac-Zimmermann

Molecular and cell biology
Protein chemistry, HPLC fractionation and 2 D gel electrophoresis
Immunofluorescence and confocal microscopy analysis
MALDI and ESI Mass Spectrometry, mass fingerprinting
Quantitative shotgun global proteomics LC-MS/MS proteomics profiling
Organelle separation and subcellular proteomics
SILAC and label-free proteomics profiling
Proteomics bioinformatics and protein association analysis

Page last modified on 18 oct 13 10:44