Centre for Nephrology
Nephrology is part of the Division of Medicine

Medicine News

Graduate Careers and Research Day 1st December 2014

Published: Oct 31, 2014 11:32:22 AM

Myelin vital for learning new practical skills

Published: Oct 20, 2014 9:38:58 AM

The Challenge of Chronic Pain 11-13 March 2015

Published: Oct 1, 2014 4:15:14 PM

Techniques

PHYSIOLOGY

Principal Investigator: Professor Robert Unwin, Dr Joanne Marks, Dr Anselm Zdebik

Renal micropuncture techniques
In vivo and isolated gut loop (transport) techniques
Brush border membrane vesicle uptake technique
Electrophysiology: twin electrode voltage clamp (TEVC), ion-selective microelectrodes, TEVC combined with pH in oocytes, calcium and pH imaging in mammalian cells
Extracellular recording of neuronal activity
2-photon (calcium) imaging
Expression profiling, surface expression, proteomics

LIPID GROUP

Principal Investigator: Dr Xiong-Zhong Ruan

Lipid biochemistry, cellular cholesterol and fatty acid assay, LDL purification
Molecular biology
Cell biology: renal and vascular cell culture
Molecular imaging: confocal microscopy
Animal models of dyslipidaemia and inflammation

PROTEOMICS & MOLECULAR CELL DYNAMICS GROUP

Principal Investigator: Professor Jasminka Godovac-Zimmermann

Molecular and cell biology
Protein chemistry, HPLC fractionation and 2 D gel electrophoresis
Immunofluorescence and confocal microscopy analysis
MALDI and ESI Mass Spectrometry, mass fingerprinting
Quantitative shotgun global proteomics LC-MS/MS proteomics profiling
Organelle separation and subcellular proteomics
SILAC and label-free proteomics profiling
Proteomics bioinformatics and protein association analysis

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