Centre for Molecular Cell Biology

Key Publications

1 Minogue S, Chu KME, Westover EJ, Covey DF, Hsuan JJ and Waugh MG. (2010) Relationship between phosphatidylinositol 4-phosphate synthesis, membrane organisation and lateral diffusion of PI4KIIα at the trans-Golgi network. J. Lipid Res. 51 2314-2324
2 Srirajaskanthan S, Caplin ME, Waugh MG, Watkins J, Meyer T, Hsuan JJ and Beaumont NJ. (2010) Identification of Mac-2BP as a putative marker of neuroendocrine tumors from the analysis of cell line secretomes. Mol. Cell. Proteomics 9 656-666 
3 Simons JP, Al-Shawi R, Minogue S, Waugh MG, Wiedemann C, Evangelou S, Loesch A, Sihra TS, King R, Warner TT and Hsuan JJ. (2009) Loss of phosphatidylinositol 4-kinase 2α activity causes late onset degeneration of spinal cord axons. Proc. Natl. Acad. Sci. U.S.A. 106 11535-11539
4 Waugh MG and Hsuan JJ. (2009) Preparation of Membrane Rafts. Meth. Mol. Biol. 462 403-414
5 Waugh MG, Minogue S and Hsuan JJ. (2009) Quantification of Multiple Phosphatidylinositol 4-Kinase Isozyme Activities in Cell Extracts. Meth. Mol. Biol. 462 279-289
6 Hsuan JJ, Waugh MG and Minogue S. (2008) Phosphatidylinositol 4-kinase type II-alpha. UCSD-Nature Molecule Pages doi:10.1038/mp.a002426.01
7 Minogue S and Hsuan JJ. (2008) Phosphatidylinositol 4-kinase type II-beta. UCSD-Nature Molecule Pages doi:10.1038/mp.a003141.01
8 Hsuan J and Waugh MG. (2007) RdgB beta. UCSD-Nature Molecule Pages doi:10.1038/ mp.a003867.01
9 Minogue S, Waugh MG, De Matteis MA, Stephens DJ, Berditchevski F and Hsuan JJ. (2006) Phosphatidylinositol 4-kinase is required for endosomal trafficking and degradation of the EGF receptor. J. Cell Sci. 119 571-581
10 Waugh MG, Minogue S, Chotai D, Berditchevski F and Hsuan JJ. (2006) Lipid and peptide control of phosphatidylinositol 4-kinase IIα in Golgi-endosomal rafts. J. Biol. Chem. 281 3757–3763
11 Waugh MG, Minogue S, Blumenkrantz D, Anderson JS and Hsuan JJ. (2003) Identification and characterisation of differentially-active pools of type II phosphatidylinositol 4-kinase in unstimulated A431 cells. Biochem. J. 376 497-503
12 Waugh MG, Minogue S, Anderson JS, Balinger A, Blumenkrantz D, Calnan DP, Cramer R and Hsuan JJ. (2003) Localization of a highly active pool of type II phosphatidylinositol 4-kinase in a p97/VCP-rich fraction of the endoplasmic reticulum. Biochem. J. 373 57-63
13 Minogue S, Anderson JS, Waugh MG, dos Santos M, Corless S, Cramer R and Hsuan JJ. (2001) Cloning of a human type II phosphatidylinositol 4-kinase reveals a novel lipid kinase family. J. Biol. Chem. 276 16635-16640
14 Hsuan J and Cockcroft S. (2001) The PITP family of phosphatidylinositol transfer proteins. Genome Biol. 2 3011.1-3011.8
15 Fullwood Y, dos Santos M and Hsuan JJ. (1999) Cloning and characterization of a novel human phosphatidylinositol transfer protein, rdgBß. J. Biol. Chem. 274 31553-31558
16 Waugh MG, Lawson D and Hsuan JJ. (1999) Epidermal growth factor receptor activation is localized within low buoyant density, non-caveolar, membrane domains. Biochem. J. 337 591-597
17 Waugh MG, Lawson D, Tan SK and Hsuan JJ. (1998) Phosphatidylinositol 4-phosphate synthesis in immunoisolated caveolae-like vesicles and low buoyant density non-caveolar membranes. J. Biol. Chem. 273 17115-17121
18 Hsuan JJ, Minogue S. and dos Santos M. (1998) Phosphoinositide 4- and 5-kinases and the cellular roles of phosphatidylinositol 4,5-bisphosphate. Adv. Cancer Res. 74 167-216
19 Woscholski R, Finan P, Radley E, Totty NF, Sterling AE, Hsuan JJ, Waterfield MD and Parker PJ. (1997) Synaptojanin is the major constitutively active phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase in rodent brain. J. Biol. Chem. 272 9625-9628
20 Hsuan JJ and Tan SK. (1997) Growth factor dependent phosphoinositide signalling. Int. J. Biochem. Cell Biol. 29 415-435
21 Carvajal JJ, Pook MA, dos Santos M, Doudney K, Hillerman R, Minogue S, Williamson R, Hsuan JJ and Chamberlain S. (1996) The Friedreich's ataxia gene encodes a novel phosphatidylinositol-4-phosphate 5-kinase. Nature Genet. 14 157-162
22 Fensome A, Cunningham E, Tan SK, Swigart P, Thomas G, Hsuan J and Cockcroft S. (1996) ARF and PITP restore GTPgS-stimulated protein secretion from granulocytic HL60 cells depleted of cytosol. Curr. Biol. 6 730-738
23 Cunningham E, Tan SK, Swigart P, Hsuan J and Cockcroft S. (1996) The yeast and mammalian isoforms of phosphatidylinositol transfer protein can all restore phospholipase C-mediated inositol lipid signalling in cytosol-depleted RBL-2H3 and HL60 cells. Proc. Natl. Acad. Sci. USA 93 6589-6593
24 Stoyanov B, Volinia S, Hanck T, Rubio I, Loubtchenkov M, Malek D, Stoyanova S, Vanhaesebroeck B, Dhand R, Nürnberg B, Gierschik P, Seedorf K, Hsuan JJ, Waterfield MD and Wetzker R. (1995) Cloning and characterisation of a G protein-activated human phosphatidylinositide-3 kinase. Science 269 690-693
25 Cullen PJ, Hsuan JJ, Truong O, Letcher AJ, Jackson TR, Dawson AP and Irvine RF. (1995) A high affinity Ins(1,3,4,5)P4-specific binding protein in platelets is a member of the GAP1 family. Nature 376 527-530
26 Divecha N, Truong O, Hsuan JJ, Hinchliffe KA and Irvine RF. (1995) The cloning and sequence of the C isoform of PtdIns4P 5-kinase. Biochem. J. 309 715-719
27 Kauffmann-Zeh A, Thomas GMH, Ball A, Prosser S, Cunningham E, Cockcroft S and Hsuan JJ. (1995) Requirement for phosphatidylinositol transfer protein in epidermal growth factor signaling. Science 268 1188-1190
28 Kauffmann-Zeh A, Klinger R, Endemann G, Waterfield MD, Wetzker R and Hsuan JJ. (1994) Regulation of human type II phosphatidylinositol kinase activity by epidermal growth factor-dependent phosphorylation and receptor association. J. Biol. Chem. 269 31243-31251
29 Hsuan JJ, Totty NF, Truong O and Waterfield MD. (1994) Purification of phosphatidylinositol 3-kinase by SDS-agarose gel electrophoresis and HPEC. Prot. Pept. Lett. 1 25-32
30 Cockcroft S, Thomas GMH, Fensome A, Geny B, Cunningham E, Totty NF, Truong O and Hsuan JJ. (1994) Phospholipase D: a downstream effector of ARF in granulocytes. Science 263 523-526
31 Thomas GMH, Cunningham E, Fensome A, Totty NF, Truong O, Hsuan JJ and Cockcroft S. (1993) An essential role for PI-transfer protein in phospholipase C-mediated inositol lipid signalling. Cell 74 919-928
32 Hiles ID, Otsu M, Volina S, Fry MJ, Gout I, Dhand R, Panayotou G, Ruiz-Larrea F, Thompson A, Totty NF, Hsuan JJ, Courtneidge SA, Parker PJ and Waterfield MD. (1992) Phosphatidylinositol 3-kinase: structure and expression of the catalytic subunit. Cell 70 419-429
33 Wetzker R, Klinger R, Hsuan JJ, Fry M, Kauffmann-Zeh A, Muller E, Frunder H and Waterfield M. (1991) Purification and characterization of phosphatidylinositol 4-kinase from human erythrocyte membranes. Eur. J. Biochem. 200 179-185
34 Otsu M, Hiles I, Goot I, Fry MJ, Ruiz- Larrea F, Panayotou G, Thompson A, Dhand R, Hsuan JJ, Totty N, Smith AD, Morgan SJ, Courtneidge S, Parker PJ and Waterfield MD. (1991) Characterization of two related 85kd proteins containing SH2 and SH3 domains that bind to receptor tyrosine kinases and polyoma virus middle T antigen/pp60c-src complexes, and associates with phosphatidylinositol 3-kinase. Cell 65 91-104