XClose

Lungs for Living Research Centre

Home
Menu

Techniques

The Lungs for Living centre covers a range of molecular and cell biology techniques including 2D and 3D culture of epithelial cells, and models of cancer and other lung diseases, both in vitro and in vivo. The image below shows an in vitro culture of human stem cell organoids.    

Human stem cell organoids

We have worked hard on improving imaging of the airways, and in 2013 published the first paper using a whole mount technique on lungs tissue (images shown below).

Wholemount

Wholemount image of lung tissue

Wholemount - zoomed

Zoomed in area of the wholemount

We also use the CRISPR/Cas9 method of genetic manipulation to study the effects of specifically changing the expression of genes thought to be critical in lung cancer development. The images below show normal lung epithelial cells (below left, p53 stained in white) compared to cells with a CRISPR/Cas9 targeted deletion of tumour suppressor protein p53 (below right).

Control cells

p53-negative cells