Reciprocal signalling drives dendritic cell migration and lymph node expansion via CLEC-2/Podoplanin interactions
Thu, 13/03/2014 - 16:00
Host cell Invasion by Toxoplasma: Insights on how parasite overcome the host cell cortical constraints?
Wed, 19/03/2014 - 16:00
Stochastic or deterministic patterning in the Drosophila visual system
Mon, 24/03/2014 - 16:00
Syntax in C. elegans locomotion: A systems approach to behaviour
Mon, 31/03/2014 - 16:00
Mon, 07/04/2014 - 16:00
Thu, 24/04/2014 - 16:00
Molecular mechanims of Wnt signalling
Mon, 19/05/2014 - 16:00
Biodiversity and environment: methodological approaches in a theory-poor and data-rich area of applied research
Mon, 09/06/2014 - 16:00
Past Seminars and Events
uMentor: An introduction to UCL's new mentoring scheme
Mon, 10/03/2014 - 16:00
The role of CORVET and HOPS complex components in organizing the endo-lysosomal system
Mon, 03/03/2014 - 16:00
Stochastic signal encoding strategies in single cells
Mon, 24/02/2014 - 16:00
Sainsbury Laboratory, Cambridge University, Cambridge, UK,
Gene expression can be surprisingly dynamic and heterogeneous. This variability in gene expression, even in a clonal population of cells grown under the same condition, has been observed in diverse organisms, from mammalian stem cells to bacteria. It remains unclear how this variability is generated and what function it can serve.
We are using the B. subtilis general stress response regulator sigB as a model system to understand how heterogeneous cell states can be generated. By carrying out time-lapse microscopy of cells expressing a reporter of sigB activity, we discovered that sigB expression occurs in discrete pulses. The regulation of these pulses is input dependent. Energy stress causes sigB to be activated in continuous stochastic pulses. Environmental stress, however, results in a single pulse of sigB activation. By using a combination of mathematical modelling and synthetic biology techniques we were able to understand the mechanism of these two different dynamical regulation schemes. We are now testing whether pulsing is a common regulatory strategy for the cell.
Nanoscale characterisation of living systems by atomic force microscopy
Mon, 17/02/2014 - 16:00
Atomic force microscopy (AFM) is a unique tool to resolve molecular and submolecular structure of living systems without the need for crystallisation, freezing or drying. Its resolving power can be illustrated by our visualisation of the DNA double helix in aqueous solution. Its main advantages, however, lie in its ability to probe (bio)physical characteristics such as kinetics (by real-time imaging) and nanomechanics of functional molecules and molecular complexes. I will illustrate the former case with our experimental results on membrane perforation by peptides/proteins, and the latter by our biophysical characterisation of the transport barrier in intact nuclear pore complexes.
Myelin plasticity and motor skills learning
Mon, 10/02/2014 - 16:00
From a connectome to functional neural maps: brain-wide calcium-imaging in C. elegans
Mon, 03/02/2014 - 16:00
Sorting out protein traffic: Ubiquitin-mediated endocytosis and ESCRT-mediated endosomal sorting
Dr. Scott Emr
Thu, 30/01/2014 - 16:00
Viva talk "Cellular analysis of HIV transmission from primary macrophages"