Robin Ketteler Research Group

1998 Diploma, Biochemistry, Free University Berlin, Germany
2002 PhD, Max-Planck-Institute for Immunobiology, Freiburg, Germany
Robin Ketteler
Tel: 020 3108 4063
Fax: 020 7679 7805
Previous Posts: 
2002-2009 Postodoctoral Fellow Massachusetts General Hospital, Boston USA

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Welcome to the Ketteler lab website! If you are interested in our research, please stay on this site. If you are interested in the high-throughput screening services that we offer, please click here.

The Ketteler lab is interested in the regulation of cancer signaling pathways. We employ high-throughput screening and novel biomolecular tools to investigate growth factor signaling cascades and to identify therapeutic targets for anti-cancer strategies. The two main research projects are:

1) Identification of novel Grb2 mediated signaling pathways

The small adaptor molecule Grb2 links growth receptor signaling to intracellular signal transduction cascades such as the MAP kinase pathway. In order to identify novel growth factor receptors and mediators, we use whole genome high-throughput screening approaches utilizing cDNA expression vectors and siRNA knockdown libraries. Grb2 tagged with the green fluorescent protein serves as a cellular marker for functional translocation upon activation of surface receptors or direct binding to Grb2. This approach enabled us to identify several novel binding partners for Grb2 that we currently characterize in further detail. For an overview of the methods used, please click here.

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In order to better characterize image-based screening data, we are developing bioinformatic tools to identify and classify sub-cellular structures in high-throughput screening applications using 3D segmentation and visualization tools.

2) Identification of signaling cascades that regulate autophagy as therapeutic opportunity

Autophagy is an evolutionary conserved protein degradation pathway that is essential for cellular homeostasis and cell viability. Autophagy is enhanced under nutrient-limiting conditions such as amino acid starvation and can be induced by treatment with rapamycin, an inhibitor of the mammalian TOR complex. Autophagy has been implicated in tumour suppressive, as well as tumour promoting mechanisms. The molecular regulation of autophagy is not very well understood.

 

 

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We have recently developed a cell-based assay for autophagy that is amenable to high-throughput screening. The method measures proteolytic cleavage of a tripartite sensor protein by the autophagy protease ATG4B. Activation of ATG4B results in release of Gaussia luciferase from cells that can be non-invasively harvested from cellular supernatants. In order to better understand the signaling cascades involved in the activation of autophagy, we are screening whole genome siRNA libraries for factors that enhance or reduce the activity of ATG4B. In addition, we are performing small molecule library screening to identify potential therapeutic lead compounds.

 

Back to Molecular Cell Biology.

Lab Members: 
Joana Costa
Christin Luft
Niccolo Pengo
Julia Petschnigg
Krisna Prak
Publications
Jump to: 2014 | 2013 | 2012 | 2011 | 2010 | 2008 | 2005 | 2004 | 2003 | 2002 | 2001 | 2000 | 1999 | 1997 | 1996
Number of items: 42.

2014

Ferraro, F; Kriston-Vizi, J; Metcalf, DJ; Martin-Martin, B; Freeman, J; Burden, JJ; ... Cutler, DF; + view all (2014) A Two-Tier Golgi-Based Control of Organelle Size Underpins the Functional Plasticity of Endothelial Cells. Dev Cell 10.1016/j.devcel.2014.03.021.

Ferraro, F; Martin-Martin, B; Westmoreland, D; Dyer, CE; Cutler, DF; Kriston-Vizi, J; ... Burden, JJ; + view all (2014) A two-tier golgi-based control of organelle size underpins the functional plasticity of endothelial cells. Developmental Cell , 29 (3) 292 - 304. 10.1016/j.devcel.2014.03.021.

Luft, C; Freeman, J; Elliott, D; Al-Tamimi, N; Kriston-Vizi, J; Heintze, J; ... Ketteler, R; + view all (2014) Application of Gaussia luciferase in bicistronic and non-conventional secretion reporter constructs. BMC Biochem , 15 (1) 14 - ?. 10.1186/1471-2091-15-14.

Stevenson, NL; Martin-Martin, B; Freeman, J; Kriston-Vizi, J; Ketteler, R; Cutler, DF; (2014) G protein-coupled receptor kinase 2 moderates recruitment of THP-1 cells to the endothelium by limiting histamine-invoked Weibel-Palade body exocytosis. J Thromb Haemost , 12 (2) 261 - 272. 10.1111/jth.12470. Green and gold open access
file

Stevenson, NL; Martin-Martin, B; Freeman, J; Kriston-Vizi, J; Ketteler, R; Cutler, DF; (2014) G protein-coupled receptor kinase 2 moderates recruitment of THP-1 cells to the endothelium by limiting histamine-invoked Weibel-Palade body exocytosis. J Thromb Haemost , 12 (2) 261 - 272. 10.1111/jth.12470.

2013

Foldes, G; Matsa, E; Kriston-Vizi, J; Leja, T; Amisten, S; Kolker, L; ... Harding, S; + view all (2013) Key differences in hypertrophic signalling in hESC-and hIPSC-derived cardiomyocytes. In: HUMAN GENE THERAPY. (pp. A9 - A9).

Foldes, G; Matsa, E; Kriston-Vizi, J; Leja, T; Mioulane, M; Vauchez, K; ... Harding, SE; + view all (2013) Key differences in hypertrophic signalling between hESC- and hIPSC-derived cardiomyocytes. In: EUROPEAN HEART JOURNAL. (pp. 279 - 279).

Heintze, J; Luft, C; Ketteler, R; (2013) A CRISPR CASe for high-throughput silencing. Frontiers in Genetics , 4 , Article 193. 10.3389/fgene.2013.00193. Green and gold open access
file

Kriston-Vizi, J; Luft, C; Freeman, J; Ketteler, R; (2013) High-complexity screening in 3D cancer model systems. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE , 32 S17 - S17.

2012

Freeman, J; Kriston-Vizi, J; Lindenschmidt, I; Gewinner, C; Ketteler, R; (2012) Activation of autophagy limits ovarian cancer cell line growth. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE , 30 S16 - S16.

Freeman, J; Kriston-Vizi, J; Seed, B; Ketteler, R; (2012) A high-content imaging workflow to study Grb2 signaling complexes by expression cloning. J Vis Exp (68) 10.3791/4382.

Ketteler, R; (2012) On programmed ribosomal frameshifting: the alternative proteomes. Front Genet , 3 242 - ?. 10.3389/fgene.2012.00242.

Marine, S; Freeman, J; Riccio, A; Axenborg, ML; Pihl, J; Ketteler, R; Aspengren, S; (2012) High-throughput transfection of differentiated primary neurons from rat forebrain. J Biomol Screen , 17 (5) 692 - 696. 10.1177/1087057112439233.

McCoy, LE; Quigley, AF; Strokappe, NM; Bulmer-Thomas, B; Seaman, MS; Mortier, D; ... Weiss, RA; + view all (2012) Potent and broad neutralization of HIV-1 by a llama antibody elicited by immunization. J Exp Med , 209 (6) 1091 - 1103. 10.1084/jem.20112655. Gold open access

2011

Freeman, J; Kriston-Vizi, J; Lindenschmidt, I; Haider, M; Ketteler, R; (2011) Autophagy protease ATG4B as a target in drug discovery. INT J MOL MED , 28 S72 - S72.

Ketteler, R; (2011) Molecular drug targets in autophagy. European Pharmaceutical Review , 16 (2)

2010

Ketteler, R; (2010) The Feynman Trajectories: Determining the Path of a Protein Using Fixed-Endpoint Assays. J BIOMOL SCREEN , 15 (3) 321 - 326. 10.1177/1087057109357116.

Ketteler, R; (2010) High-Throughput screening approaches to dissect cancer signaling pathways. INT J MOL MED , 26 S56 - S56.

Ketteler, R; Tomov, V; Neunkirchner, A; Xie, QA; Pickl, WF; Seed, B; (2010) Host-encoded reporters for the detection and purification of multiple enveloped viruses. J VIROL METHODS , 167 (2) 178 - 185. 10.1016/j.jviromet.2010.04.002.

2008

Becker, V; Sengupta, D; Ketteler, R; Ullmann, GM; Smith, JC; Klingmuller, U; (2008) Packing Density of the Erythropoietin Receptor Transmembrane Domain Correlates with Amplification of Biological Responses. BIOCHEMISTRY-US , 47 (45) 11771 - 11782. 10.1021/bi801425e.

Ketteler, R; Seed, B; (2008) Quantitation of autophagy by luciferase release assay. AUTOPHAGY , 4 (6) 801 - 806.

Ketteler, R; Sun, ZR; Kovacs, KF; He, WW; Seed, B; (2008) A pathway sensor for genome-wide screens of intracellular proteolytic cleavage. GENOME BIOL , 9 (4) , Article R64. 10.1186/gb-2008-9-4-r64.

2005

Becker, V; Ketteler, R; Schilling, M; Baumann, U; Heinrich, AC; Ruan, W; ... Klingmuller, U; + view all (2005) A single residue in the transmembrane domain of the erythropoietin receptor regulates lipid raft localization and signal amplification. In: EUROPEAN JOURNAL OF CELL BIOLOGY. (pp. 45 - 45). URBAN & FISCHER VERLAG

2004

Zimmermann, S; Glaser, S; Ketteler, R; Waller, CF; Klingmuller, U; Martens, UM; (2004) Effects of telomerase modulation in human hematopoietic progenitor cells. STEM CELLS , 22 (5) 741 - 749.

2003

Ketteler, R; Moghraby, CS; Hsiao, JG; Sandra, O; Lodish, HF; Klingmuller, U; (2003) The cytokine-inducible Scr homology domain-containing protein negatively regulates signaling by promoting apoptosis in erythroid progenitor cells. J BIOL CHEM , 278 (4) 2654 - 2660. 10.1074/jbc.M211236200. Gold open access

Na, SY; Lichtenthaler, S; Xavier, R; Radoja, S; Ketteler, R; Holmes, T; Seed, B; (2003) Pathway dissection by automated expression screens. In: FASEB JOURNAL. (pp. A149 - A149). FEDERATION AMER SOC EXP BIOL

2002

Ketteler, R; Glaser, S; Sandra, O; Martens, UM; Klingmüller, U; (2002) Enhanced transgene expression in primitive hematopoietic progenitor cells and embryonic stem cells efficiently transduced by optimized retroviral hybrid vectors. Gene Ther , 9 (8) 477 - 487. 10.1038/sj.gt.3301653.

Ketteler, R; Glaser, S; Sandra, O; Martens, UM; Klingmuller, U; (2002) Enhanced transgene expression in primitive hematopoietic progenitor cells and embryonic stem cells efficiently transduced by optimized retroviral hybrid vectors. GENE THER , 9 (8) 477 - 487. 10.1038/sj/gt/3301653.

Ketteler, R; Heinrich, AC; Offe, JK; Becker, V; Cohen, J; Neumann, D; Klingmuller, U; (2002) A functional green fluorescent protein-tagged erythropoietin receptor despite physical separation of JAK2 binding site and tyrosine residues. J BIOL CHEM , 277 (29) 26547 - 26552. 10.1074/jbc.M202287200. Gold open access

Martens, UM; Glaser, S; Ketteler, R; Waller, CF; Klingmuller, U; Zimmermann, S; (2002) Telomerase transduction in cord blood CD34+cells interferes with cellular differentiation and does not increase their proliferative capacity. In: BLOOD. (pp. 716A - 716A). AMER SOC HEMATOLOGY

2001

Ketteler, R; Sandra, O; Klingmuller, U; (2001) Improved purification and ex vivo expansion of hematopoietic progenitor cells promotes high transduction rates and efficient transgene expression by novel hybrid retroviral vectors. BLOOD , 98 (11) 117B - 118B.

Kubatzky, KF; Ruan, WM; Gurezka, R; Cohen, J; Ketteler, R; Watowich, SS; ... Klingmuller, U; + view all (2001) Self assembly of the transmembrane domain promotes signal transduction through the erythropoietin receptor. CURR BIOL , 11 (2) 110 - 115.

Zimmermann, S; Glaser, S; Ketteler, R; Waller, CF; Klingmuller, U; Martens, UM; (2001) Telomere elongation in hematopoietic progenitor cells. BLOOD , 98 (11) 276A - 276A.

2000

Ketteler, R; Glaser, S; Martens, UM; Klingmueller, U; (2000) Transgene expression by retroviral vectors harboring the MoMLV-primer binding site is restricted in embryonic stem cells, but not in hematopoietic stem cells. BLOOD , 96 (11) 431A - 431A.

Zimmermann, S; Glaser, S; Ketteler, R; Lange, W; Klingmuller, U; Martens, UM; (2000) Telomere length homeostasis in hTERT transduced cord blood CD34+cells. BLOOD , 96 (11) 69A - 69A.

Zouboulis, CC; Katsantonis, J; Ketteler, R; Treudler, R; Kaklamani, E; Hornemann, S; ... Orfanos, CE; + view all (2000) Adamantiades-Behcet's disease: interleukin-8 is increased in serum of patients with active oral and neurological manifestations and is secreted by small vessel endothelial cells. ARCH DERMATOL RES , 292 (6) 279 - 284.

1999

Ketteler, R; Sandra, O; Esser, S; Klingmueller, U; (1999) Introducing the small sh2-domain containing protein CIS by a novel retroviril vector impairs proliferation but not differentiation of erythroid progenitors. BLOOD , 94 (10) 268A - 268A.

Zouboulis, CC; Katsantonis, J; Treudler, R; Ketteler, R; Kaklamani, E; Hornemann, S; ... Orfanos, CE; + view all (1999) Interleukin-8 is increased in serum of patients with active adamantiades-Behcet's disease and is released by small vessel endothelial cells. J INVEST DERMATOL , 113 (3) 487 - 487.

1997

Hornemann, S; Seltmann, H; Kodelja, V; Ketteler, R; Orfanos, CE; Zouboulis, CC; (1997) Interleukin-1 alpha in cultured human sebocytes: mRNA and protein expression and influence of lipopolysaccharides. J INVEST DERMATOL , 109 (3) 453 - 453.

Seiffert, K; Fritsch, M; Ketteler, R; Orfanos, CE; Zouboulis, CC; (1997) A novel method for separation of neutral lipids and phospholipids from cultured cells and their supernatants. J INVEST DERMATOL , 108 (3) 23 - 23.

1996

Ketteler, R; Treudler, R; Orfanos, CE; Zouboulis, CC; (1996) Cultured endothelial cells secrete interleukin-8 after stimulation with serum of patients with Adamantiades-Behcet's disease. J INVEST DERMATOL , 107 (3) 166 - 166.

Treudler, R; Ketteler, R; Orfanos, CE; Zouboulis, CC; (1996) Increased serum levels of interleukin-1 beta and basic fibroblast growth factor in severe Adamantiades-Behcet's disease. J INVEST DERMATOL , 107 (3) 165 - 165.

This list was generated on Fri Aug 22 07:06:55 2014 BST.