Our research is focused on the development of experimental technology to study biological processes that fall outside of the resolving power of conventional cell biology studies.
We specially focus on applications to super-resolution (SR) microscopy. SR is non-trivial and requires a multidisciplinary approach for its development and application. Additionally, a novel realm of analytical and modelling tools needs to be established to resolve the new level of information retrieved from cellular structure and behaviour at the nanoscale. Together with collaborators we have tackled some of these challenges, developing fundamental technologies ranging the areas of optical microscopy, quantitative image analysis, photoswitchable fluorescence and the analysis of molecular territories within cells.
These same principles seed and guide our research group. Both training and bringing together specialised scientists in the fields of cell biology, physics, applied mathematics, optics, and computer science, unified by with the common desire to apply their expertise into the study of biological systems. The group is set in a highly cooperative environment, with a large percentage of its emphasis directed into developing super-resolution technology and applying it to biological research – for example the nanoscale study of the spatio-temporal associations between NF-κB and endocytic signalling regulators.
Some examples of our work:
Gaussian spot detection example through QuickPALM - an ultra-fast algorithm for photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) applied to the PSFs of a common digital camera filming the Eiffel Tower stochastic blinking.
For more info visit http://code.google.com/p/quickpalm/.
QuickPALM: 3D dSTORM of microtubules ~40nm resolution
High-content super-resolution of T-cells. A collaboration with Dr. Helena Soares at Institut Pasteur, Paris.