Our research is focused on the development of experimental technology to study biological processes that fall outside of the resolving power of conventional cell biology studies. We specially focus on applications to super-resolution (SR) microscopy. SR is non-trivial and requires a multidisciplinary approach for its development and application. Additionally, a novel realm of analytical and modelling tools needs to be established to resolve the new level of information retrieved from cellular structure and behaviour at the nanoscale. Together with collaborators we have tackled some of these challenges, developing fundamental technologies ranging the areas of optical microscopy, quantitative image analysis, photoswitchable fluorescence and the analysis of molecular territories within cells.
These same principles seed and guide our research group. Both training and bringing together specialised scientists in the fields of cell biology, physics, applied mathematics, optics, and computer science, unified by with the common desire to apply their expertise into the study of biological systems. The group is set in a highly cooperative environment, with a large percentage of its emphasis directed into developing super-resolution technology and applying it to biological research.
|Tarantino, N; Tinevez, J-Y; Crowell, EF; Boisson, B; Henriques, R; Mhlanga, M; ... Laplantine, E; + view all Tarantino, N; Tinevez, J-Y; Crowell, EF; Boisson, B; Henriques, R; Mhlanga, M; Agou, F; Israel, A; Laplantine, E; - view fewer (2014) TNF and IL-1 exhibit distinct ubiquitin requirements for inducing NEMO-IKK supramolecular structures. The Journal of Cell Biology , 204 (2) 231 - 245. 10.1083/jcb.201307172. |
|Soares, H; Henriques, R; Sachse, M; Ventimiglia, L; Alonso, MA; Zimmer, C; ... Alcover, A; + view all Soares, H; Henriques, R; Sachse, M; Ventimiglia, L; Alonso, MA; Zimmer, C; Thoulouze, MI; Alcover, A; - view fewer (2013) Regulated vesicle fusion generates signaling nanoterritories that control T cell activation at the immunological synapse. J Exp Med , 210 (11) 2415 - 2433. 10.1084/jem.20130150. |
|Ehsani, S; Santos, JC; Rodrigues, CD; Henriques, R; Audry, L; Zimmer, C; ... Enninga, J; + view all Ehsani, S; Santos, JC; Rodrigues, CD; Henriques, R; Audry, L; Zimmer, C; Sansonetti, P; Tran Van Nhieu, G; Enninga, J; - view fewer (2012) Hierarchies of host factor dynamics at the entry site of Shigella flexneri during host cell invasion. Infect Immun , 80 (7) 2548 - 2557. 10.1128/IAI.06391-11. |
|Gomes-Santos, CS; Itoe, MA; Afonso, C; Henriques, R; Gardner, R; Sepúlveda, N; ... Mota, MM; + view all Gomes-Santos, CS; Itoe, MA; Afonso, C; Henriques, R; Gardner, R; Sepúlveda, N; Simões, PD; Raquel, H; Almeida, AP; Moita, LF; Frischknecht, F; Mota, MM; - view fewer (2012) Highly Dynamic Host Actin Reorganization around Developing Plasmodium Inside Hepatocytes. PLoS One , 7 (1) , Article e29408. 10.1371/journal.pone.0029408. |
|Herbert, S; Henriques, R; (2012) Enhanced epifluorescence microscopy by uniform and intensity optimized illumination. Cytometry A , 81 (4) 278 - 280. 10.1002/cyto.a.21177. |
|Herbert, S; Soares, H; Zimmer, C; Henriques, R; (2012) Single-molecule localization super-resolution microscopy: deeper and faster. Microsc Microanal , 18 (6) 1419 - 1429. 10.1017/S1431927612013347. |
|Lelek, M; Di Nunzio, F; Henriques, R; Charneau, P; Arhel, N; Zimmer, C; (2012) Superresolution imaging of HIV in infected cells with FlAsH-PALM. Proc Natl Acad Sci U S A , 109 (22) 8564 - 8569. 10.1073/pnas.1013267109. |
|Henriques, R; Griffiths, C; Hesper Rego, E; Mhlanga, MM; (2011) PALM and STORM: unlocking live-cell super-resolution. Biopolymers , 95 (5) 322 - 331. 10.1002/bip.21586. |
|Henriques, R; Lelek, M; Fornasiero, EF; Valtorta, F; Zimmer, C; Mhlanga, MM; (2010) QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nat Methods , 7 (5) 339 - 340. 10.1038/nmeth0510-339. |
|Barreiro, LB; Henriques, R; Mhlanga, MM; (2009) High-throughput SNP genotyping: combining tag SNPs and molecular beacons. Methods Mol Biol , 578 255 - 276. 10.1007/978-1-60327-411-1_17. |
|Caetano-Lopes, J; Nery, AM; Henriques, R; Canhão, H; Duarte, J; Amaral, PM; ... Fonseca, JE; + view all Caetano-Lopes, J; Nery, AM; Henriques, R; Canhão, H; Duarte, J; Amaral, PM; Vale, M; Moura, RA; Pereira, PA; Weinmann, P; Abdulghani, S; Souto-Carneiro, M; Rego, P; Monteiro, J; Sakagushi, S; Queiroz, MV; Konttinen, YT; Graça, L; Vaz, MF; Fonseca, JE; - view fewer (2009) Chronic arthritis directly induces quantitative and qualitative bone disturbances leading to compromised biomechanical properties. Clin Exp Rheumatol , 27 (3) 475 - 482. |
|Henriques, R; Mhlanga, MM; (2009) PALM and STORM: what hides beyond the Rayleigh limit? Biotechnol J , 4 (6) 846 - 857. 10.1002/biot.200900024. |
|Rino, J; Braga, J; Henriques, R; Carmo-Fonseca, M; (2009) Frontiers in fluorescence microscopy. Int J Dev Biol , 53 (8-10) 1569 - 1579. 10.1387/ijdb.072351jr. |
|Henriques, RJDSD; QuickPALM. [Software]. |