For image analysis, we use Acapella (PerkinElmer) and IMAGEJ. The use of open software such as IMAGEJ has the advantage that the source code is available and it is FREE! The availability of multiple plugins makes this a very convenient software for our daily use. The image analysis is built on a very powerful hardware with 64 CPU for parallel processing of data (400 GB memory). For data storage and image handling, we use the Columbus Conductor.