Opera Phenix Plus
The Phenix Plus shares all the features of the Opera Phenix but has a higher imaging frame rate and is equipped with an injector module for the addition of reagents and compounds while capturing images. As a result, it is well-suited for observing rapid-response assays. Specifications:
| Perkin Elmer Opera Phenix
The Opera Phenix is a confocal spinning disc microscope that has the capability to automatically acquire images in multiwell plates and slides. The automated acquisition advances the imaging process and allows the researcher to study their project in an impartial way. Our users image a wide variety of samples on the Phenix, e.g. single layer cell lines, multilayer neuronal cultures, tissue sections and organoids. The Phenix is equipped with CO2 and temperature control features, enabling the capture of live cell images and time courses. A plate handler and incubator allow the automated imaging of multiple plates. Specifications:
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Tecan Freedom Evo 200
The Tecan Freedom Evo is a tip based liquid handler we use for the automation of many of our screens. The system is adaptable and easy to set up. Specifications:
| Beckman Biomek platform
For complex experiments and screens, we use our Biomek liquid handling platform which is equipped with two NXp liquid handlers, one using a 96/384 channel head and one using an 8-channel head. Both use disposable tips for aspiration, dispensing and dilution. The platform also contains tilting and shaker units, a vacuum unit, cooling and heating blocks, stackers, an incubator and a tubing based plate washer and dispenser. This set up allows for the standardized and automated conduction of longer experimental, cell culturing and immunostaining protocols. Specifications:
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Labcyte Echo 550 with CellAccess system
The Labcyte Echo is an acoustic liquid dispenser, which transfers reagents from a source plate or library plate into assay plates via sound waves. This technology allows the transfer of very small volumes (as small as 2.5nl). This method of dispensing is faster and more accurate than traditional liquid handling. We use it in screens to transfer compounds, siRNA/crRNA and other small volume reagents. For larger screens, source and assay plates can be stored in plate stackers and fed into the Echo automatically via the cell access system. | PerkinElmer Envision II
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Agilent Seahorse XFE96
The Seahorse measures extracellular O2 and pH levels in cells plated in a 96 well plate format. During the measurement process several modulators of respiration and metabolism are injected into the culture solution. In this way researchers can investigate metabolic processes including mitochondrial respiration, glycolytic function and ATP production. | Other liquid handlers
We have several other tip based and tubing based liquid handlers which support smaller and larger scale experiments and screens. They include the Agilent Bravo, The Formulatrix Tempest and other portable tubing based dispensers. |
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