The Baum Lab

Andrea Dimitracopoulos (PhD student, @andimi_): 
Using a combination of genetic and physical approaches, I am studying how cell rounding and the actin cortex affect mitotic spindle assembly and positioning in order to ensure efficient and timely cell division.

In flattened Rap1*-expressing HeLa cells, chromosomes are lost from the nascent spindle or become arrayed along the side of the forming spindle (compared to control).



Christina Dix (PhD student): Investigating polarity changes and the role of cdc42 during mitotic entry and exit in cell culture and in vivo.
This image demonstrates RPE1 cells expressing zyxin:GFP.






Pelin Candarlioglu (Postdoctoral researcher): I aim to utilize molecular biophysical and cellular biology techniques to investigate how circulating cancer stem cells (CTCs) control their cell cycle and cell fate, in order to further understand how CTCs contribute to metastasis.

HeLa cells stably expressing H2B-mCherry and alpha-tubulin-GFP were filmed at 10x magnification through time lapse microscopy every 30 minutes for 5 days to determine cell cycle in 3 consecutive cell divisions as a model for cancer cell cycle kinetics.





Ginger Hunter (Postdoctoral researcher): I take a genetic, biophysical and cell biological approach to investigate how cytoskeletal mechanics and regulation contribute to the efficiency of Notch-Delta mediated patterning of the Drosophila notum.

Via the process of lateral inhibition, these 3 cells in the Drosophila pupal notum have switched from an epithelial cell fate to a neural cell fate and will eventually contribute to the formation of bristles on the adult fly thorax. F-actin (red), cell membrane (green).





Jigna Patel (Research assistant): In a follow up to a genetic screen performed in the lab, I am studying asymmetry in cell division time and cytokinesis defects.

A typical cytokinesis failure leading to multinucleation in HeLa cells. DNA (blue), tubulin (green), actin (red), cytochrome C (gray).




Maxine Lam (PhD student): Studying the role of mechanical forces in mitosis in the developing fly notum



Helen Matthews (Postdoctoral researcher) studies mitotic rounding, the shape change that animal cells undergo when they enter mitosis. She aims to discover how this shape change is co-ordinated by the cell cycle machinery and the actin cytoskeleton and how this process changes during cancer progression.



Nunu  Mchedlishvili (Postdoctoral researcher) is investigating role of nuclear envelope breakdown in the coordination of cytoskeleton remodeling upon entry into mitosis.
A HeLa cell expressing H2B-mRFP and alpha-tubulin-GFP undergoing mitosis.