Electron Microscopy Techniques

fission yeast.jpg

  • Conventional aldehyde fixation and resin embedding
  • High pressure freezing with freeze substitution
  • Tokuyasu cryo preparation
  • Room temperature sectioning: semi-thins, ultra-thins, serial sectioning
  • Cryo-sectioning
  • Whole mounts
  • Rip-offs
  • Pre-embedding immunolabelling
  • Correlative Light and Electron microscopy (CLEM): organism, cellular and organelle levels. XY and XZ orientations.
  • 3D EM: Reconstruction of tilt tomograms, TEM images of serial
    sections, Serial Block Face SEM images or Focused ion beam SEM images.









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