UCL Lipid Signalling

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  • Carvou N, Holic R, Li M, Futter C, Skippen A, Cockcroft S. (2010) Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPβ is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum J Cell Sci. 2010 Apr 15;123(Pt 8):1262-73. Epub 2010 Mar 23.


  • Yanase Y, Carvou N, Frohman MA, Cockcroft S. (2009) Reversible bleb formation in mast cells stimulated with antigen is Ca2+-calmodulin -dependent and bleb size is regulated by ARF6. Biochem J. Oct 2009; 425(1):179�193.
  • Leber W, Skippen A, Fivelman QL, Bowyer PW, Cockcroft S, Baker DA. (2009) A unique phosphatidylinositol 4-phosphate 5-kinase is activated by ADP-ribosylation factor in Plasmodium falciparum. Int J Parasitol. May 2009; 39(6):645-53
  • Cockcroft S. (2009) Phosphatidic acid regulation of phosphatidylinositol 4-phosphate 5-kinases. Biochim Biophys Acta. 2009 Sep;1791(9):905-12
  • Kolman P, Pica A, Carvou N, Boyde A, Cockcroft S, Loesch A, Pizzey A, Simeoni M, Capasso G, Unwin RJ.(2009) Insulin uptake across the luminal membrane of the rat proximal tubule in vivo and in vitro. Am J Physiol Renal Physiol. May 2009;296: F1219-F1226
  • Cockcroft S. (2009) Measurement of phosphatidylinositol and phosphatidylcholine binding and transfer activity of the lipid transport protein PITP. Methods Mol Biol. 2009;462:363-77


  • Shadan S, Holic R, Carvou N, Ee P, Li M, Murray-Rust J, Cockcroft S. (2008) Dynamics of Lipid Transfer by Phosphatidylinositol Transfer Proteins in Cells. Traffic. Sep 9; (10):1743-56.
  • Cosker KE, Shadan S, van Diepen M, Morgan C, Li M, Allen-Baume V, Hobbs C, Doherty P, Cockcroft S, Eickholt BJ. (2008) Regulation of PI3K signalling by the phosphatidylinositol transfer protein PITPalpha during axonal extension in hippocampal neurons. J Cell Sci. Mar 15;121(Pt 6):796-803.
  • Saiardi A, Cockcroft S. (2008) Human ITPK1: a reversible inositol phosphate kinase/phosphatase that links receptor-dependent phospholipase C to Ca2+-activated chloride channels. Sci. Signal. Jan 29; 1(4):pe5.


  • Stace C, Manifava M, Delon C, Coadwell J, Cockcroft S, Ktistakis NT. (2007) PA binding of phosphatidylinositol 4-phosphate 5-kinase. Adv Enzyme Regul. Nov;48(1):55-72
  • Carvou N, Norden AG, Unwin RJ, Cockcroft S. (2007) Signalling through phospholipase C interferes with clathrin-mediated endocytosis. Cell Signal. Jan;19(1):42-51.
  • Fensome-Green A, Stannard N, Li M, Bolsover S, Cockcroft S. (2007) Bromoenol lactone, an inhibitor of Group V1A calcium-independent phospholipase A(2) inhibits antigen-stimulated mast cell exocytosis without blocking Ca(2+) influx. Cell Calcium. Feb;41(2):145-53.


  • Morgan CP, Allen-Baume V, Radulovic M, Li M, Skippen A, Cockcroft S. (2006) Differential expression of a C-terminal splice variant of phosphatidylinositol transfer protein beta lacking the constitutive-phosphorylated Ser262 that localizes to the Golgi compartment. Biochem J. Sep 15;398(3):411-21.
  • Hyvola N, Diao A, McKenzie E, Skippen A, Cockcroft S, Lowe M. (2006) Membrane targeting and activation of the Lowe syndrome protein OCRL1 by rab GTPases. EMBO J. Aug 23;25(16):3750-61.
  • Fensome-Green A, Cockcroft S. (2006) Reconstitution system based on cytosol-depleted cells to study the regulation of phospholipase D. Methods Mol Biol. 2006;332:299-310.
  • Skippen A, Swigart P, Cockcroft S. (2006) Measurement of phospholipase C by monitoring inositol phosphates using [3H]inositol-labeling protocols in permeabilized cells. Methods Mol Biol. 2006;312:183-93.


  • Hunt, A. N., Skippen, A. J., Koster, G., Postle, A. D. and Cockcroft, S. (2004) Acyl chain-based molecular selectivity for HL60 cellular phosphatidylinositol and of phosphatidylcholine by phosphatidylinositol transfer protein α. Biochim Biophys Acta - Mol. Cell Biol. of Lipids 1686: 50-60
  • Morgan, C. P., Skippen, A. J. , Segui, B., Ball, A., Allen-Baume, A., Larijani, B., Murray-Rust, J., McDonald, N., Sapkota, G., Morrice, N., and Cockcroft, S. (2004) Phosphorylation of a distinct structural form of PITPα at Ser166 by protein kinase C disrupts receptor-mediated phospholipase C signalling by inhibiting delivery of phosphatidylinositol to membranes. J. Biol. Chem 279: 47159-47171
  • Morgan, C. P., Insall, R., Haynes, L and Cockcroft, S. (2004) Identification of phospholipase B from Dictyostelium discoideum reveals a new lipase family present in mammals, flies and nematodes, but not yeast. Biochem. J. 382: 441-449
  • Tilley, S. J., Skippen, A. J., Murray-Rust, J., Swigart, P. M., Stewart, A., Morgan, C. P., Cockcroft, S., and McDonald, N. Q. (2004) Structure-Function Analysis of Phosphatidylinositol Transfer Protein Alpha Bound to Human Phosphatidylinositol. Structure 12: 317-326.
  • Pandey, V., Mihara, S., Fensome-Green, A., Bolsover, S., and Cockcroft, S. (2004) Monomeric IgE Stimulates NFAT Translocation Into the Nucleus, a Rise in Cytosol Ca2+, Degranulation, and Membrane Ruffling in the Cultured Rat Basophilic Leukemia-2H3 Mast Cell Line. J. Immunology 172: 4048-4058


  • Sarri, E., R. Pardo, A. Fensome-Green, and S. Cockcroft. (2003) Endogenous PLD2 localizes to the plasma membrane of RBL 2H3 cells and can be distinguished from ARF-stimulated PLD activity by its specific sensitivity to oleic acid. Biochem. J. 369: 319-329
  • Larijani, B., V. Allen-Baume, C. P. Morgan, M. Li, and S. Cockcroft. (2003) EGF regulation of PITPα and PITPβ dynamics at the plasma membrane monitored by fluorescence lifetime imaging.Curr.Biol. 73: 78-84


  • O'Luanaigh, N., R. Pardo, D. Jones, A. Fensome, V. Allen-Baume, D. Jones, M. R. Holt, and S. Cockcroft. (2002)Continual production of PA by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells. Mol.Biol.cell 13: 3730-3746
  • Segui, B., V. Allen-Baume, and S. Cockcroft. (2002) Phosphatidylinositol transfer protein-beta displays minimal sphingomyelin transfer activity and is not required for biosynthesis and traffficking of sphingomyelin. Biochem. J. 366: 23-34
  • Skippen. A, D. H. Jones, C. P. Morgan, M. Li, and S. Cockcroft. (2002) Mechanism of ADP-ribosylation factor-stimulated phosphatidylinositol 4,5-bisphosphate synthesis in HL60 cells. J.Biol.Chem. 277: 5823-5831
  • Coburn, R. F., D. H. Jones, C. P. Morgan, C. B. Baron, and S. Cockcroft. (2002) Spermine increase phosphatidylinositol 4,5-bisphosphate content in permeabilized and non-permeabilized HL60 cells. Biochim Biophys Acta 1584: 20-30
  • Kular, A. Chaudhary, G. Prestwich, P. Swigart, R. Wetzker, and S. Cockcroft. (2002) Co-operation of phosphatidylinositol transfer protein with phosphoinositide 3-kinase (in vitro). Adv.Enz.Reg. 42: 53-61


  • Bolsover, S.R., O. Ibrahim, N. O'Luanaigh, H. Williams, and S. Cockcroft. (2001) Use of fluorescent dyes with green fluorescent protein and its variants: problems and solutions. Biochem. J.. 356: 345-352


  • Jones, D. H., Morris, J. B., Morgan, C. P., Kondo, H., Irvine, R. F., Cockcroft, S. (2000) Type I Phosphatidylinositol 4-phosphate 5-kinase directly interacts with ADP-ribosylation factor 1 and is responsible for phosphatidylinositol 4,5-bisphosphate synthesis in the Golgi compartment. J.Biol.Chem. 275, 13962-13966
  • Swigart, P., Insall, R. H., Wilkins, A., Cockcroft, S. (2000) Purification and cloning of phosphatidylinositol transfer proteins from Dictyostelium discoideum: Homologues of both mammalian PITPs and S. cerevisiae Sec14p are found in the same cell. Biochem. J. 347, 837-843
  • Way, G., O'Luanaigh, N., Cockcroft, S. (2000) Activation of exocytosis by cross-linking of the IgE receptor is dependent on ARF-regulated phospholipase D in RBL-2H3 mast cells: Evidence that the mechanism of activation is via regulation of PIP2 synthesis. Biochem. J. 346, 63-70


  • Jones, D. H., Bax, B., Fensome, A., Cockcroft, S. (1999) ADP ribosylation factor 1 mutants identify a phospholipase D effector region and reveal that phospholipase D participates in lysosomal secretion but is not sufficient for recruitment of coatomer I. Biochem. J. 341, 185-192
  • Leino, L., Forbes, L., Segal, A., Cockcroft, S. (1999) Reconstitution of GTPγS-induced NADPH oxidase activity in streptolysin-O permeabilisedneutrophils by specific cytosol fractions. Biochem.Biophys.Res.Commun. 265, 29-37
  • Phillips, S. E., Sha, B., Topalof, L., Xie, Z., Alb, J. G., Klenchin, V. A., Swigart, P., Cockcroft, S., Martin, T. F. J., Luo, M., Bankaitis, V. A. (1999) Yeast Sec14p deficient in phosphatidylinositol transfer activity is functional in vivo. Mol.Cell Biol. 4, 187-197
  • Whatmore, J., Wiedemann, C., Somerharju, P., Swigart, P., Cockcroft, S. (1999) Resynthesis of phosphatidylinositol in permeabilised neutrophils following phospholipase Cβ activation: Transport of the intermediate, phosphatidic acid from the plasma membrane to the endoplasmic reticulum for phosphatidylinositol resynthesis is not dependent on soluble lipid carriers or vesicular transport. Biochem. J. 341, 435-444


  • Fensome, A., Whatmore, J., Morgan, C. P., Jones, D., Cockcroft, S. (1998) ADP-ribosylation factor and Rho proteins mediate fMLP-dependent activation of phospholipase D in human neutrophils. J.Biol.Chem. 273, 13157-13164
  • Norman, J. C., Jones, D., Holt, M. R., Barry, S. T., Cockcroft, S., Critchley, D. R. (1998) ARF1 mediates paxillin recruitment to focal adhesions and potentiates rho-stimulated stress fiber formation in intact and permeabilised Swiss 3T3 fibroblasts. J.Cell Biol. 143, 1981-1995


  • Allen, V., Swigart, P., Cheung, R., Cockcroft, S., Katan, M. (1997) Regulation of inositol lipid-specific phospholipase Cδ by changes in Ca2+ ion concentrations. Biochem. J. 327, 545-552
  • Hara, S., Swigart, P., Jones, D., Cockcroft, S. (1997) The first 5 amino acids of the carboxy terminus of phosphatidylinositol transfer protein a (PITPα) play a critical role in inositol lipid signaling: transfer activity of PITP is essential but not sufficient for restoration of phospholipase C signaling. J.Biol.Chem. 272, 14909-14913
  • Kular, G., Loubtchenkov, M., Swigart, P., Whatmore, J., Ball, A., Cockcroft, S., Wetzker, R. (1997) Co-operation of phosphatidylinositol transfer protein with phosphoinositide 3-kinase (gamma) in the formylmethionyl-leucylphenylalanine-dependent production of phosphatidylinositol 3,4,5 trisphosphate in human neutrophils. Biochem. J. 325, 299-301
  • Morgan, C. P., Sengelov, H., Whatmore, J., Borregaard, N., Cockcroft, S. (1997) ARF-regulated phospholipase D activity localizes to secretory vesicles and mobilizes to the plasma membrane following fMetLeuPhe stimulation of human neutrophils. Biochem. J. 325, 581-585
  • Panaretou, C., Domin, J., Cockcroft, S., Waterfield, M. D. (1997) Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150-PtdIns 3-kinase complex. J.Biol.Chem. 272, 2477-2485
  • Prosser, S., Sarra, R., Swigart, P., Ball, A., Cockcroft, S. (1997) Deletion of 24 amino acids from the C-terminus of phosphatidylinositol transfer protein causes loss of phospholipase C-mediated inositol lipid signalling. Biochem. J. 324, 19-23


  • Cunningham, E., Tan, S. W., Swigart, P., Hsuan, J., Bankaitis, V., Cockcroft, S. (1996) The yeast and mammalian isoforms of phosphatidylinositol transfer protein can all restore phospholipase C-mediated inositol lipid signalling in cytosol-depleted RBL-2H3 and HL60 cells. Proc.Natl.Acad.Sci.U.S.A. 93, 6589-6593
  • Fensome, A., Cunningham, E., Prosser, S., Tan, S. K., Swigart, P., Thomas, G., Hsuan, J., Cockcroft, S. (1996) ARF and PITP restore GTPγS-stimulated protein secretion from cytosol-depleted HL60 cells by promoting PIP2 synthesis. Current Biol. 6, 730-738
  • Vinggaard, A. M., Jensen, T., Morgan, C. P., Cockcroft, S., Hansen, H. S. (1996) Didecanoylphosphatidylcholine is a superior substrate for assaying mammalian phospholipase D. Biochem. J. 319, 861-864
  • Whatmore, J., Morgan, C. P., Cunningham, E., Collison, K. S., Willison, K. R., Cockcroft, S. (1996) ADP-ribosylation factor1-regulated phospholipase D is localized at the plasma membrane and intracellular organelles in HL60 cells. Biochem. J. 320, 785-794


  • Cunningham, E., Thomas, G. M. H., Ball, A., Hiles, I., Cockcroft, S. (1995) Phosphatidylinositol transfer protein dictates the rate of inositol trisphosphate production by promoting the synthesis of PIP2. Current Biol. 5, 775-783
  • De Vries, K. J., Heinrichs, A. A. J., Cunningham, E., Brunink, F., Westerman, J., Somerharju, P. J., Cockcroft, S., Wirtz, K. W. A., Snoek, G. T. (1995) An isoform of the phosphatidylinositol transfer protein transfers sphingomyelin and is associated with the golgi system. Biochem. J. 310, 643-649
  • Greasley, S. E., Jhoti, H., Teahan, C., Solari, R., Fensome, A., Thomas, G. M. H., Cockcroft, S., Bax, B. (1995) The structure of rat ADP-ribosylation factor-1 (ARF-1) complexed to GDP determined from two different crystal forms. Nature Structural Biol. 2, 797-806
  • Kauffmann-Zeh, A., Thomas, G. M. H., Ball, A., Prosser, S., Cunningham, E., Cockcroft, S., Hsuan, J. J. (1995) Requirement for phosphatidylinositol transfer protein in Epidermal Growth Factor signalling. Science 268, 1188-1190


  • Cockcroft, S., Thomas, G. M. H., Fensome, A., Geny, B., Cunningham, E., Gout, I., Hiles, I., Totty, N. F., Troung, O., Hsuan, J. J. (1994)Phospholipase D: A downstream effector of ARF in granulocytes. Science 263, 523-526


  • Cockcroft, S., Taylor, A. J., Judah, J. D. (1985) Subcellular localisation of inositol lipid kinases in rat liver. Biochem Biophys Acta 845, 163-170. Download PDF (582KB)

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