|Stem Cell Therapy and Cell Transplantation
|Transplanted cells integrated into the adult eye
Transplanted cells integrated into degenerating retina
repair by transplantation of photoreceptor precursors
In a recent paper we showed
that photoreceptor precursor cells isolated from a neonatal (P1)
gfp transgenic mouse can integrate successfully into the outer nuclear
layer of the retina of neonatal and adult wild-type mice (MacLaren
et al, Nature 444: 203-7).
To assess whether
successfully transplanted cells were light-responsive and functionally
connected to downstream targets, we used two techniques; pupillometry
and extracellular field potential recordings from the ganglion cell
layer (GCL) in treated rhodopsin knock-out mice and peripherin deficient
and field potential recordings showed increased sensitivities in
the retinas receiving precursor cell transplants compared to untreated
and sham-injected eyes.
The results from these experiments indicate that the adult retina
canfunctionally incorporate precursors of rod photoreceptor cells. These cells will differentiate and form functional synaptic connections with downstream target cells in the recipient retina. To translate these results into a functional stem cell therapy, it is essential to identify a source of stem cells that can be cultured and differentiated to a stage similar to the P1 rod precursor cells used in these experiments. Meanwhile, we aim to improve on these results by further optimisation of the transplantation methods.
Isolation, culture and differentiation of adult stem cells
This page last modified
18 December, 2012