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Biological Mass Spectrometry Centre

IMPORTANT -Collaborations and co-authorship on any grants or publications resulting from work carried out in the biological mass spectrometry centre

For the simple identification of proteins by ESI-QTOF sequencing, then our group do not stipulate co-authorship on any subsequent publications but we do require acknowledgement of the Biological Mass Spectrometry centre at ICH.

However, if the data generated requires any subsequent in-depth analyses of the data by any members of the centre e.g. looking for changes in post-translational modifications, interpretation of spectra or quantitation, then co-authorship on any publications or grant applications, by a member of the respective groups involved in the analysis will be required (proteomics, metabolomics or IR mass spectrometry division).

Services and costs

Proteomics

All enquires regarding Proteomics analysis should be directed to Dr Kevin Mills

Sequencing identification of proteins excised from gels and analysis by ESI QTOF MS (from 2D-PAGE and 1D-gels)

The in-gel tryptic digestion of proteins and their extraction from the gel pieces can be performed according to this

Provided that the protein is in the UniProt database we will guarantee an identification for coomaasie stained proteins. If we do not get an identification we will process another band at no extra cost. For silver stained proteins we are willing to attempt an identification but cannot guarantee it.

 The laboratory accepts samples for analysis in 1D gel-bands or 2D-PAGE spots and will fully process the sample.  Please contact the laboratory prior to sending any proteins for analyses.

Costs are £150 per sample or £100 per sample for more than 10 samples in 1 batch

Identification and quantitation of proteins within mixtures (MSe Label-free Quantitation)

The laboratory accepts samples for the quantitation of the major proteins in mixtures after immunoprecipitation, complexes, protein: protein interactions, subcellular organelles, tissues and cell extracts.  Please contact the laboratory prior to sending any proteins for analyses.

Costs are £200 per sample for UCL departments and £300 per sample for non-UCL customers. If you require particular peptides from a mixture to be sequenced or looking for possible post-translational modifications, then please contact us in advance.

2D-PAGE / DIGE biomarker screen

Price includes full experimental and computer analyses of the sample sets.

Costs are £150 per sample. Each gel can analyse 2-3 samples depending on study design.

Subsequent silver staining of the 2D DiGE gel is £100 per gel.

Metabolomics

Plasma bile acid analysis by GC-MS

For plasma bile acid analyses we require 1-2 ml of lithium heparin plasma which can be posted to our lab without special precautions if it will arrive within 24 hours of dispatch (orange top and separated from the red cells).  However, if the transfer will take longer than 24 hours, all samples should be sent on card-ice and should not be scheduled to arrive at ICH on the weekend. 

Possible cases of inborn errors of bile acid synthesis can be discussed with

or the sending of samples can be discussed prior to dispatch with

Costs are £100 per sample.

Urine bile acids by ESI-MS/MS

For a urine bile acid analysis we require > 2 ml of a random urine which can be posted to our lab without special precautions if it will arrive within 24 hours of dispatch. However, if the transfer will take longer than 24 hours, all samples should be sent on dry-ice and should not be scheduled to arrive at ICH on the weekend.

Possible cases of inborn errors of bile acid synthesis can be discussed with

or the sending of samples can be discussed prior to dispatch with

Costs are £80 per sample.

Prenatal Diagnoses of inborn errors of cholesterol synthesis (Smith Lemli Opitz Syndrome / Desmosterolosis)

All prenatal diagnoses should be discussed prior to sampling with

Costs are £250 per analyses.

Plasma sterol analysis for inborn errors of cholesterol synthesis or phytosterolaemia by GC-MS

We require > 200 µl of lithium heparin plasma which can be posted to our lab without special precautions if it will arrive within 24 hours of dispatch (orange top and separated from the red cells). However, if the transfer will take longer than 24 hours, all samples should be sent on dry-ice and should not be scheduled to arrive at ICH on the weekend. Possible cases of inborn errors of cholesterol synthesis can be discussed with

or the sending of samples can be discussed prior to dispatch with

Costs are £80 per sample.

Alpha Amino Adipic Semialdehyde (AASA) by ESI-MS/MS

We require > 1 ml of a random urine which can be posted to our lab without special precautions if it will arrive within 16 hours of dispatch.  However, AASA is particularly susceptible to breakdown when unfrozen, if the transfer will take longer than 16 hours, all samples should be sent on dry-ice and should not be scheduled to arrive at ICH on the weekend.  Please make sure the sample is not too dilute as it makes quantitation difficult and analysis may need repeating.

Analysis of AASA as a diagnosis of Pyridoxine dependent epilepsy can be discussed with

  • Professor Peter Clayton (tel: 020 7905 2628)

or the sending of samples can be discussed prior to dispatch with

Costs are £150 per sample.

Postal address for metabolomics samples:

Professor Peter Clayton (c/o Catherine Clubley)
Biochemistry Department
Institute of Child Health
30 Guilford Street
London. WC1N 1EH

Isotope ratio Mass Spectrometry

For further information, please contact

Sample submission form

Costings

Page last modified on 04 sep 12 11:43