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Proteomic and Diagnostic Services

Proteomics Service

In addition to translational based research our centre is also part of the UCL Proteomics Platform which collaborates and provides access to biological mass spectrometry within UCL and industry alike. The Biological Mass Spectrometry Centre provides a proteomic identification service. This is useful for identifications from Coomassie or silver stained gel bands of for identifications from IP pull down experiments where the beads can be directly digested.

  • We can provide a limited screening service using shotgun label free quantitation. This involves absolute quantitation of samples. Data is provided in an exel spreadsheet either fentomoles or nanograms of protein for you yourselves to perform comparative analysis

We do NOT provide bioinformatics for this type of limited analysis. Full biomarker screening with licensed software is only part of a collaborative agreement.

  • For the simple identification of proteins by ESI-QTOF sequencing, our group do not stipulate co-authorship on any subsequent publications but we do require acknowledgement of the Biological Mass Spectrometry Centre at ICH
  • Before any service analysis is performed an IDT number must be provided. External institutes and industry clients are subject to VAT and a purchase order must be received before any analysis is initiated
  • Whilst we endeavour to always deliver results in a timely manner. Mass spectrometers are complex machines and do break down therefore we do not commit to specific deadlines or time frames for proteomics service results

Please contact Mr Ivan Doykov for an informal discussion of the services we offer or if you wish to send samples for analyses.

Prices

Identification of proteins from gel bands:
  • Provided that the protein is in the UniProt database we will guarantee an identification for coomaasie stained proteins. If we do not get identification we will process another band at no extra cost. For silver stained proteins we are willing to attempt identification but cannot guarantee it.The laboratory accepts samples for analysis in 1D gel-bands or 2D-PAGE spots and will fully process the sample. Please contact the laboratory prior to sending any proteins for analyses. 

£100/band, if more than 10 bands: £75/band

Identification of proteins from complex mixtures:
  • (ie. Directly from beads from an IP pull down experiment) IP experiments: £150 this includes 2 digestions one of eluted proteins from the beads (proteins of interest) and another of a direct digestion of the eluted beads to confirm antibody or protein binding to beads.

Quantitation by label free: £200/sample

Diagnostic Services

The BMSC accepts samples for specialised analytical clinical analyses on a research basis for:

Urine and plasma globotriaosylceramide (Gb3) also known as ceramide trihexoside (CTH) and globotriaosylsphingosine (lyso-Gb3) in urine and plasma

We are one of the main centres for the analysis of cerebrotrixdase/Gb3/GL3 and lyso-CTH/Gb3/in urine and plasma and shortly we will be initiating the assay from bloodspots. These are the accumulative compounds that are the hallmark of Fabry disease. The BMSC was the first centre to establish the measurement of Gb3 for Fabry disease (kevs paper) in 200??by LC-MS/MS. Gb3 and lyso-Gb3 are often monitored in Fabry disease and we routinely analyse samples for clinical trials for Genzyme Sanofi.

If you wish to send samples for analysis please contact Mr Ivan Doykov if you wish to enquire about measuring Gb3 and lyso-Gb3 regarding a potential clinical trial please contact Dr Kevin Mills.

Costs are £100 per sample.

Urine bile acids by ESI-MS/MS

For a urine bile acid analysis we require > 2 ml of a random urine which can be posted to our lab without special precautions if it will arrive within 24 hours of dispatch. However, if the transfer will take longer than 24 hours, all samples should be sent on dry-ice and should not be scheduled to arrive at ICH at the weekend.

Possible cases of inborn errors of bile acid synthesis can be discussed with

· Professor Peter Clayton (tel: 020 7905 2628)

Costs are £80 per sample.

Prenatal Diagnoses of inborn errors of cholesterol synthesis (Smith Lemli Opitz Syndrome / Desmosterolosis)

All prenatal diagnoses should be discussed prior to sampling with

· Professor Peter Clayton (tel: 020 7905 2628). 

Costs are £250 per analyses.

Plasma sterol analysis for inborn errors of cholesterol synthesis or phytosterolaemia by GC-MS

We require > 200 µl of lithium heparin plasma which can be posted to our lab without special precautions if it will arrive within 24 hours of dispatch (orange top and separated from the red cells). However, if the transfer will take longer than 24 hours, all samples should be sent on dry-ice and should not be scheduled to arrive at ICH at the weekend. Possible cases of inborn errors of cholesterol synthesis can be discussed with

· Professor Peter Clayton (tel: 020 7905 2628)

Costs are £80 per sample.

Alpha Amino Adipic Semialdehyde (AASA) by ESI-MS/MS

We require > 1 ml of a random urine which can be posted to our lab without special precautions if it will arrive within 16 hours of dispatch.  However, AASA is particularly susceptible to breakdown when unfrozen, if the transfer will take longer than 16 hours, all samples should be sent on dry-ice and should not be scheduled to arrive at ICH at the weekend. Please make sure the sample is not too dilute as it makes quantitation difficult and analysis may need repeating.

Analysis of AASA as a diagnosis of Pyridoxine dependent epilepsy can be discussed with

· Professor Peter Clayton (tel: 020 7905 2628)

Costs are £150 per sample.

Postal address for metabolomics samples:

Professor Peter Clayton  G23 - 1C - Biochemistry,   UCL Institute of Child Health,  30 Guilford Street,  London, WC1N 1EH

Page last modified on 19 sep 14 21:14