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Laboratory services

Biological mass spectrometry centre

Proteomics

Matrix-Assisted Laser Desorption Ionisation Quadrupole-Time of Flight Mass Spectrometry (MALDI QTOF MS) and Electrospray Quadrupole - Time of Flight Mass Spectrometry (ESI-QTOF)

The Unit benefited from a SRIF award that involved the procurement of a dual-source (MALDI and ESI) time-of-flight mass spectrometer. MALDI QTOF MS is a very sensitive ionisation technique that is used primarily within the unit for identification of proteins excised from gels and the analysis of carbohydrate structures.

ESI QTOF MS is a dynamic technique that allows the coupling of the mass spectrometer to a liquid chromatography interface (nanoLC). ESI QTOF MS is used for the sequencing / identification of proteins and / or locating post-translational modifications such as phosphorylation / glycosylation / alkylation etc. The ESI QTOF MS is also used in conjunction with 2-dimensional liquid chromatography to study changes in protein expression as a complement to existing 2D-PAGE analyses.
Read more on Electrospray Quadrupole - Time of Flight Mass Spectrometry...

Typhoon Trio Scanner (2D-PAGE / 2D-DIGE)

The department also has a Typhoon fluorescent scanner which is used for 2D-DIGE analysis and for detecting quantative changes in protein expression.

2D-PAGE / 2D-DIGE

The Centre has several IPG-PHOR isoelectric focusing, 1D and 2D-PAGE electrophoresis units including a Protean II Mulitcell and Ettan Dalt-12 gel systems. There is also a Safe Imager (blue light transilluminator) for viewing ultra-violet sensitive stains (Sypro Ruby, Deep Purple etc.).
Read more about electrophoresis...

Nano-High Performance Liquid Chromatography Unit

Solvent and sample delivery to the ESI-Q-TOF is performed using a NanoAcquity HPLC

Tissue Imaging / MALDI Imaging

The Centre has recently acquired tissue imaging capability

Click here to learn more about the various proteomic technologies.

Page last modified on 08 mar 12 10:17