Institute of Child Health
- Athena SWAN
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ICH Embryonic stem cell / Chimera production facility
The ICH ES/Chimera Production Facility provides a customised knock-out and knock-in service.
ES cell manipulation
We will supply the ES cells and carry out the electroporation, selection and freezing of ES clones. Regretfully, we can neither generate targeting constructs nor carry out the screen of the ES cell clones for you.
Blastocyst injection and chimera production
We will inject between 100-120 blastocysts using 2-4 targeted clones
and perform 8-10 transfers into recipient mice. To date, using this
protocol, we have generated 26 mouse models. We cannot guarantee the
generation of chimeric mice or germline transmission, but so far we have
obtained chimeric mice in the vast majority of cases:
Chimeras will be maintained by us until they are 3 weeks old and then we will send them to you at your expense.