Flow Cytometry Core Facility
Data files Transfer:
The facility now has a shared drive for data transfer purpose therefore all USB memory sticks and external hard drives are banned and shouldn't be brought and used in the facility.
You can mount the shared drive on your computer following instruction on this word document or if you are logging onto a UCL computer on the UCL network i.e at EDI, IoN etc, you can download a program called ShareMounter from this link.
When you run the program you can choose to map the s:\ drive and login with you UCL username and password.
If you are logging into a computer which has been Sync'd and Shared on another SLMS site, you should automatically be able to see the S drive.
If you are working from home and would like to access the shared drive, you have 2 options: you can use the Desktop@UCLAnywhere service or install the VPN client on you home computer and then download sharemounter to connect to the S drive.
If you have difficulty connecting to flow shared drive let us know or get in touch with ICH IT Support.
New Charges Effective From 1st October 2013:
The ICH/GOS Flow Cytometry Facility is run on a fully cost-recovered basis. We have been operating since 2008 and since then have not raised charges to users. However, we are not immune to the economic circumstances and in order to preserve the service for the next three years, we need to raise our charges. This isn’t something we are doing lightly and we hope our user community will understand the need.Please visit section Charges and Conditions of Use to get the new charges.
The ICH/GOS Flow Cytometry Facility is run on a fully cost-recovered basis. We have been operating since 2008 and since then have not raised charges to users. However, we are not immune to the economic circumstances and in order to preserve the service for the next three years, we need to raise our charges. This isn’t something we are doing lightly and we hope our user community will understand the need.
Please visit section Charges and Conditions of Use to get the new charges.
Quite busy. Book a week in advance for convenient time.
All Other Analysers (CyAn, Array, Calibur):
Can be booked on the day.
Cell Sorter MoFlo XDP:
You are advised to book at least 1 to 2 weeks in advance for a convenient time slot.
Mac and PC Analysis Computers (FlowJo, FACSDiva, Kaluza, ModFit):
Can be booked on the day.
FACSAria III Cell Sorter
The facility has extended its cell sorting capability. We can now perform sorts on our new 5-lasers, 18-colours, 4-way FACSAria III. Most fluorochromes can be used on this instrument.
You are advised to book a week in advance for a convenient time slot
Flow Cytometry Core Facility at University College London Institute of Child Health and Great Ormond Street Hospital, London, UK
UCL ICH/GOS Flow Cytometry Core Facility provides state of the art instrumentation and expertise for cell analysis and sorting.
- Book or check availability of a flow cytometer (all users)
The facility has four cell analysers:
- BD LSRII with four lasers (350, 405, 488, and 633 nm) and 19 parameters (17 colours) detection capability
- CyAn ADP with two lasers (488 and 633 nm) and 9 parameters (7 colours) detection capability
- BD FACSArray (SORP) with 2 lasers (488 and 633 nm) and 4 colours (FITC and PE on the blue laser and APC and APC-Cy7 on the red diode).
- BD FACSCalibur with 2 lasers (488 and 633 nm) and 4 colours (FITC, PE and PECy5 or PECy7 on the blue laser and APC on the red diode)
The facility offers cell sorting on the latest technology from Beckman Coulter and Becton Dickinson, the
high speed cell sorters MoFlo XDP and FACSAria III.
MoFlo XDP sorter is equipped with three lasers:
- true UV laser (350 nm) enabling 2 colours detection
- argon blue laser (488 nm), allowing 5 colours detection
- red diode (647 nm) enabling 2 colours detection.
FACSAria III is equipped with 5 lasers:
- near UV (375 nm) and Violet (405 nm), which are co linear and can accommodate up to 6 colours.
- argon blue laser (488 nm) allowing detection of FITC and one more fluorochrome excitable by this laser
- yellow/green laser (561 nm) allowing 5 colours detection
- red laser (647 nm) allowing 3 colours detection.
Services available include:
- cell sorting
- cloning: single cell sorting
- data acquisition, analysis and interpretation
- user training
- advice on sample preparation
- user assistance and machine troubleshooting
- help with design of experiments
- sourcing of reagents and techniques; finding alternative approaches
- help with data preparation for presentation and publication
We are also open to collaboration.
The facility is located on Level 3 of the Camelia Botnar
It is open 8.00am to 11.00pm, 7 days a week.
Facility staff are available Monday to Friday, 9.00am to 6.00pm.
Please note that sorts cannot be booked before 11.00am because the cell sorter requires a calibration period before use. However, early morning (10am) and evening sorts are available by prior arrangement.
Guidelines for the use of the facility are provided on these web pages. Please ensure you have read the relevant guidelines to maximise the success and validity of your experiment.
Page last modified on 03 jul 15 08:35 by Ayad Eddaoudi