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UCL Great Ormond Street Institute of Child Health

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Flow Cytometry Core Facility

Flow Cytometry Core Facility at UCL GOS Institute of Child Health provides state of the art instrumentation and expertise for cell analysis and sorting.

Guidelines for the use of the facility are provided on these web pages. Please ensure you have read the relevant guidelines to maximise the success and validity of your experiment.


Book a CELL ANALYSER

(must register or login via PPMS)

Check availability of a CELL SORTER

(must login with flowcytometryich@googlemail.com account (requires PW that is available at the facility. You can only see availability.)

Build your FLOW PANEL

Services Available


Instruments:

Cell analysers

The facility has four cell analysers: LSRII, CyAn, FACSArray and FACSCalibur.

Cell sorters

The facility offers cell sorting on the latest technology from Beckman Coulter and Becton Dickinson, the high speed cell sorters MoFlo XDP and FACSAria III.

BD LSRII

BD LSRII

With four lasers (350, 405, 488, and 633 nm) and 19 parameters (17 colours) detection capability.

CyAn ADP

CyAn ADP

With two lasers (488 and 633 nm) and 9 parameters (7 colours) detection capability.

BD FACSArray (SORP)

BD FACSArray

With 2 lasers (488 and 633 nm) and 4 colours (FITC and PE on the blue laser and APC and APC-Cy7 on the red diode).

BD FACSCalibur

BD FACSCalibur

With 2 lasers (488 and 633 nm) and  4 colours (FITC, PE and PECy5 or PECy7 on the blue laser and APC on the red diode)

MoFlo XDP

MoFlo XDP

Equipped with three lasers:

  • true UV laser (350 nm) enabling 2 colours detection
  • argon blue laser (488 nm), allowing 5 colours detection
  • red diode (647 nm) enabling 2 colours detection.

FACSAria III

FACSAria III

Equipped with 5 lasers:

  • near UV (375 nm) and Violet (405 nm), which are co linear and can accommodate up to 6 colours.
  • argon blue laser (488 nm) allowing detection of FITC and one more fluorochrome excitable by this laser
  • yellow/green laser (561 nm) allowing 5 colours detection
  • red laser (647 nm) allowing 3 colours detection.