Flow cytometry
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managers photo3

Facility Manager

Dr Ayad Eddaoudi

Contact

Tel: (+44) 020 7905 2331
Fax: (+44) 020 7905 2810

  • Opening hours (staffed):
    9.00am-6.00pm Mon-Fri
  • Facility's location:

Room P3.016, Level 3, 
Camelia Botnar Laboratories
Great Ormond Street Hospital
85 Lamb's Conduit
London WC1N 3JH

  • Postal address:

UCL Great Ormond Street Institute of Child Health
30 Guilford Street
London WC1N 1EH

Flow Cytometers Status

LSRII 

  • Quite busy: Book a week in advance for convenient time.

All Other Analysers 

  • Array, Calibur, CyAn: Can be booked on the day.

MoFlo XDP Cell Sorter: 

  • Can be booked on the day but you are advised to book 1 week in advance for a convenient time slot.

FACSAria III Cell Sorter

  • You are advised to book at least 2 weeks in advance for a convenient time slot.

Analysis Computers 

  • FlowJo, FACSDiva, Kaluza, Summit: Can be booked on the day.

Flow cytometry

UCL GOS ICH Flow Cytometry Core Facility provides state of the art instrumentation and expertise for cell analysis and sorting.

Guidelines for the use of the facility are provided on these web pages. Please ensure you have read the relevant guidelines to maximise the success and validity of your experiment.

PPMS*:

Register, book or check availability of a flow cytometer

*Platform Pilot Management System

Services Available

  • cell sorting
  • cloning: single cell sorting
  • data acquisition, analysis and interpretation
  • user training
  • advice on sample preparation
  • user assistance and machine troubleshooting
  • help with design of experiments
  • sourcing of reagents and techniques; finding alternative approaches
  • help with data preparation for presentation and publication

We are also open to collaboration.

Cell analysers

The facility has four cell analysers:   LSRII, CyAn, FACSArray and FACSCalibur.

Cell sorters

The facility offers cell sorting on the latest technology from Beckman Coulter and Becton Dickinson, the high speed cell sorters MoFlo XDP and FACSAria III.

BD LSRII
BD LSRII

 With four lasers (350, 405, 488, and 633 nm) and 19 parameters (17 colours) detection capability.

CyAn ADP

CyAn ADP

With two lasers (488 and 633 nm) and 9 parameters (7 colours) detection capability.

BD FACSArray (SORP)
BD FACSArray

With 2 lasers (488 and 633 nm) and 4 colours (FITC and PE on the blue laser and APC and APC-Cy7 on the red diode).

BD FACSCalibur
BD FACSCalibur

With 2 lasers (488 and 633 nm) and  4 colours (FITC, PE and PECy5 or PECy7 on the blue laser and APC on the red diode)

MoFlo XDP
MoFlo XDP

.

Equipped with three lasers:

  • true UV laser (350 nm) enabling 2 colours detection
  • argon blue laser (488 nm), allowing 5 colours detection
  • red diode (647 nm) enabling 2 colours detection.
FACSAria III
FACSAria III

.

Equipped with 5 lasers:

  • near UV (375 nm) and Violet (405 nm), which are co linear and can accommodate up to 6 colours.
  • argon blue laser (488 nm) allowing detection of FITC and one more fluorochrome excitable by this laser
  • yellow/green laser (561 nm) allowing 5 colours detection
  • red laser (647 nm) allowing 3 colours detection.

Page last modified on 14 jul 16 14:50 by Ayad Eddaoudi