Dr Ayad Eddaoudi
Tel: (+44) 020 7905 2331
Fax: (+44) 020 7905 2810
- Opening hours (staffed):
- Facility's location:
Room P3.016, Level 3,
Camelia Botnar Laboratories
Great Ormond Street Hospital
85 Lamb's Conduit
London WC1N 3JH
- Postal address:
UCL Institute of Child Health
30 Guilford Street
London WC1N 1EH
UCL ICH/GOS Flow Cytometry Core Facility provides state of the art instrumentation and expertise for cell analysis and sorting.
The facility has four cell analysers:
With four lasers (350, 405, 488, and 633 nm) and 19 parameters (17 colours) detection capability
With two lasers (488 and 633 nm) and 9 parameters (7 colours) detection capability
With 2 lasers (488 and 633 nm) and 4 colours (FITC and PE on the blue laser and APC and APC-Cy7 on the red diode).
With 2 lasers (488 and 633 nm) and 4 colours (FITC, PE and PECy5 or PECy7 on the blue laser and APC on the red diode)
The facility offers cell sorting on the latest technology from Beckman Coulter and Becton Dickinson, the high speed cell sorters MoFlo XDP and FACSAria III:
MoFlo XDP sorter is equipped with three lasers:
FACSAria III is equipped with 5 lasers:
Services available include:
- cell sorting
- cloning: single cell sorting
- data acquisition, analysis and interpretation
- user training
- advice on sample preparation
- user assistance and machine troubleshooting
- help with design of experiments
- sourcing of reagents and techniques; finding alternative approaches
- help with data preparation for presentation and publication
We are also open to collaboration.
The facility is located on Level 3 of the Camelia Botnar
It is open 8.00am to 11.00pm, 7 days a week.
Facility staff are available Monday to Friday, 9.00am to 6.00pm.
Please note that sorts cannot be booked before 11.00am because the cell sorter requires a calibration period before use. However, early morning (10am) and evening sorts are available by prior arrangement.
Guidelines for the use of the facility are provided on these web pages. Please ensure you have read the relevant guidelines to maximise the success and validity of your experiment.
Page last modified on 04 aug 15 12:22