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Sequencing Facility

The facility owes its origins to a NERC equipment grant to establish a high-throughput sequencing and genotyping facility.

Fraser Simpson

Services at the UCL Sequencing Facility

The facility offers high throughput sequencing and genotyping services using an ABI3730xl DNA Analyzer located in Room 213, Darwin Building UCL. We accept 96-well plates only. GEE staff currently benefit from a service that charges only the cost of consumables.

Potential users outside GEE should feel free to discuss possible options, specialized needs, and our charging structure.  Contact gee-sequencing@ucl.ac.uk

Technical staff
Fraser Simpson
 
Wendy Hart  
Rebecca Finlay
 
Charges

UCL Sequencing is to encourage higher throughput at knockdown prices. All prices are therefore per 96-well plate or for a half plate (48 reactions. If you want to do smaller jobs, you must pay the whole/half plate charge although these are still highly competitive prices.

Sequencing
Machine-ready. i.e. sequencing reaction already done and cleaned up

Full Plate (96 wells)
UCL: £60 per 96 wells (approx. £0.60/sequence)
External: £150 per 96 wells (approx. £1.50/sequence)
Half Plate (48 wells)
UCL: £40 per 48 wells (approx. £0.80/sequence)
External: £100 per 48 wells (approx. £2.08/sequence)

Sequencing run with sequencing reaction using BigDyev3.1

Full Plate (96 wells)
UCL: £250 per 96 wells (approx. £2.50/sequence)
External: £350 per 48 wells (approx. £3.50/sequence)
Half Plate (48 wells)
UCL: £175 per 48 wells (approx. £3.60/sequence)
External: £250 per 48 wells (approx. £5.20/sequence)

NB If you want to do your own sequencing reactions (which is more economical), but don’t know how or don’t want to buy lots of Big Dye, we can advise.

Genotyping (includes microsatellites/STRs,AFLP)

Full Plate (96 wells)
UCL: £100 per 96 wells (approx. £1.00/well)
External: £160 per 96 wells (approx. £1.66/well)
Half Plate (48 wells)
UCL: £70 per 48 wells (approx. £1.46/well)
External: £110 per 48 wells (approx. £2.29/well)



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Page last modified on 30 oct 14 14:33