Tn Designation and Database listing

This website has been set up to designate Tn numbers to researchers publishing details of new transposable elements discovered in bacteria and archaea. Transposable elements are defined as “specific DNA segments that can repeatedly insert into one or more sites in one or more genomes” (Roberts et al., 2008). This definition is modified from that stated in the original nomenclature proposal (Campbell et al., 1979a; 1979b) allowing it to include the many different types of transposable elements discovered since the original proposal was published.

New individual Tn numbers should be given to elements showing < 100% deduced nucleotide sequence similarity to their closest relatives, or to elements that contain novel resistance, virulence, catabolic or other accessory genes, or novel combinations of genes or modules. If functionality is demonstrated (excision, insertion, transposition or transfer) but the entire sequence of the element is not determined the element may still be assigned a Tn number provided the known sequence is sufficiently different from previously determined sequences, as described above. The rules governing the requesting of Tn numbers are summarised in a key below.

It is appreciated that other elements such as integrated or transposable prophage and satellite prophage, group I and II introns, IStrons, inteins, and insertion sequences fit this definition of a transposable element. However, in most cases there is already a coherent nomenclature system in place and any change would cause confusion. If a novel element is found which fails to qualify for an established nomenclature scheme a Tn number should be used.

Key for determining if a Tn number is appropriate. (not appropriate for ISs or non autonomous transposable elements such as integron cassettes or MITEs) (Roberts et al., 2008).

^ain the case of composite transposon flanked by IS elements transposition or different insertion sites need to be demonstrated, as it is possible that the majority of these have never transposed.

Roberts AP, Chandler M, Courvalin P, Guédon G, Mullany P, Pembroke T, Rood JI, Smith CJ, Summers AO, Tsuda M, Berg DE. (2008) Revised Nomenclature for Transposable Genetic Elements. Plasmid. 60; 167-173.

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Assigned Tn numbers

Tn Number	Name	Organization/Address	Characteristics	Reference	Accession Numbers
Tn6000	Dr Adam Roberts	UCL Eastman Dental Institute 256 Gray's Inn Road London UK	Previously designated EfcTn1 this conjugative transposon confers tetracycline resistance [tet(S)]. It is based on a Tn916-like backbone however the integrase is more related to the integrase of Staphylococcal pathogenicity islands.	Roberts et al.,(2006) Characterization of the Ends and Target Site of a Novel Tetracycline Resistance-Encoding Conjugative Transposon from Enterococcus faecium 664.1H1. Journal of Bacteriology 188:4356-61.	DQ295784 [tet(S)],DQ370177 (left end), DQ370176 (right end).
Tn6001	Dr Sung-Pin Tseng	NTU CLS&MB Institute No. 1, Chang-Te Street Taipei Taiwan		Tseng SP et al.,(2007) Characterization of Tn6001, a Transposon-Like Element Containing the blaVIM-3-harboring Integron In450. Antimicrobial Agents and Chemotherapy. 51(11):4187-90.	EF138817
Tn6002	Dr Philip Warburton	UCL Eastman Dental Institute 256 Gray's Inn Road London UK	Tn916-like element with a 2.8kb erythromycin resistance encoding insert within orf20.	Warburton PJ et al.,(2007) Demonstration of in vivo transfer of doxycycline resistance mediated by a novel transposon. Journal of Antimicrobial Chemotherapy. 60(5):973-80	AY898750
Tn6003	Prof Maria Pia Montanari	Institute of Microbiology and Biomedical Polytechnic University of Marche Medical Ancona Italy	Tn6003; (4225 bp) contains an erm(B) (lacking the stop codon) with its peptide leader; an aminoglycoside-streptothricin resistance cluster (aadE-sat4-aphA-3 genes) (100% homology, save for a deletion of 511 nucletides in aadE); and an ORF identical to orf47 of plasmid pRE25 of E. faecalis. This element has inserted into Tn6002.	Cochetti et al.,(2007) New Tn916-related elements causing erm(B)-mediated erythromycin resistance in tetracycline susceptable pneumococci. Journal of Antimicrobial Chemotherapy. 60(1):127-31	AM410044
Tn6004	Dr Elisabetta Mantengoli	Università degli studi di siena Policlinico le scotte, viale bracci 16 Siena Italy	Tn3-like transposon containing an IS26 insertion in tnpR.	Endimiani A et al., (2007). Spread in an Italian Hospital of a clonal Acinetobacter baumannii Strain Producing the TEM-92 Extended-Spectrum Beta-Lactamase. Antimicrobial Agents and Chemotherapy. 51(6):2211-2214.
Tn6005	Dr Maurizio Labbate	Macquaarie University Balaclava Rd North Ryde Sydney NSW Australia		Labbate et al., (2008). A class 1 integron present in a human commensal has a hybrid transposition module compared to Tn402: evidence of interaction with mobile DNA from natural environments. Journal of Bacteriology. Epub ahead of print
Tn6006	Dr Maurizio Labbate	Macquaarie University Balaclava Rd North Ryde Sydney NSW Australia		As for Tn6005
Tn6007	Dr Maurizio Labbate	Macquaarie University Balaclava Rd North Ryde Sydney NSW Australia		As for Tn6005
Tn6008	Dr Maurizio Labbate	Macquaarie University Balaclava Rd North Ryde Sydney NSW Australia		As for Tn6005
Tn6009	Dr Olusegun Soge	University of Washington Department of Pathobiology Box 357238 Seattle WA USA	Tn916 element linked with a Staphylococcus aureus mer operon	Soge et al., (2008). A novel transposon, Tn6009, composed of a Tn916 element linked with a Staphylococcus aureus mer operon. Journal of Antimicrobial Chemotherapy. Epub ahead of print.	EU239355, EU399632
Tn6010	Mr Anders Norman	University of Copenhagen Sølvgade 83H Copenhagen Denmark	Composite transposon from E. coli	Norman et al., (2008) Nucleotide sequence of pOLA52: A conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid 60:59-74.	EU370913
Tn6011	Mr Anders Norman	University of Copenhagen Sølvgade 83H Copenhagen Denmark	Composite transposon from E. coli	Norman et al., (2008) Nucleotide sequence of pOLA52: A conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid 60:59-74.	EU370913
Tn6012	Dr Teruyo Ito	Juntendo University Hongo 2-1-1 Bunkyyo-ku Tokyo Japan	Putative conjugative transposon from Staphylococcus aureus.	Han et al., (2009)Identification of a novel variant of staphylococcal cassette chromosome mec, type II.5, and Its truncated form by insertion of putative conjugative transposon Tn6012. Antimicrobial Aents and Chemotherapy. 53, 2616-9	AB435014
Tn6013	Dr D. Ashley Robinson	New York Medical College Microbiology and Immunology Valhalla NY USA	Integrative conjugative element from Staphylococcus aureus. Designated ICE6013	Smyth and Robinson, 2009, Integrative and sequence characteristics of a novel genetic element, ICE6013, in Staphylococcus aureus. Journal of Bacteriology. 191: 5964-75	FJ231270
Tn6014	Miss Lisbeth de Vries	National Food Institute DTU Bülowsvej 27 Copenhagen V Denmark	Tn5801-like transposon shown to transfer horizontally from one S. aureus into the S. aureus recipient RN4220F	J Antimicrob Chemother. 2009 Sep; 64 (3): 490-500	EU918655
Tn6015	Dr Richard Eaton	Agricultural Research Service, USDA 1100 Robert E. Lee Blvd. Southern Regional Research Center New Orleans Louisiana USA
Tn6016	Prof Paul Roy	Université Laval Infectious Diseases Res. Ctr. 2705, boul. Laurier, Suite RC-709 Québec QC Canada
Tn6017	Prof Paul Roy	Université Laval Infectious Diseases Res. Ctr. 2705, boul. Laurier, Suite RC-709 Québec QC Canada
Tn6018	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia		Post and Hall. (2009) AbaR5, a large multiple-antibiotic resistance region found in Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy. 53:2667-2671	FJ172370
Tn6019	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6020	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia		Post and Hall. (2009) AbaR5, a large multiple-antibiotic resistance region found in Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy. 53:2667-2671	FJ172370
Tn6021	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6022	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6023	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6024	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6025	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6026	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6027	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6028	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6029	Ms Virginia Post	The University of Sydney Biochemistry Building G08 Sydney NSW Australia
Tn6030	Dr Hiroyuki Arai	Dept. of Biotechnology, Univ. of Tokyo Yayoi 1-1-1 Bunkyo-ku Tokyo Japan
Tn6031	Dr Timothy LaPara	University of Minnesota 500 Pillsbury Drive SE Minneapolis MN USA	A mobilizable transposon-like element containing tet(X) sharing organization features and genes with CTnDOT from Bacteroides spp.	Ghosh et al. (2009) Sphingobacterium sp. strain PM2-P1-29 harbours a functional tet(X) gene encoding for the degradation of tetracycline. Journal of Applied Microbiology 106:1336-1342.	EU864422
Tn6032	Dr Hikaru Suenaga	AIST Central 6, 1-1-1 Higashi, Tsukuba Japan
Tn6033	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6034	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6035	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6036	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6037	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6038	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6039	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6040	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6041	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6042	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6043	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6044	Mr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6045	Ms Sae Tsubakishita	Juntendo university 2-1-1 Hongo Bunkyo-ku Tokyo Japan
Tn6046	Ms Sae Tsubakishita	Juntendo university 2-1-1 Hongo Bunkyo-ku Tokyo Japan
Tn6047	Ms Sae Tsubakishita	Juntendo university 2-1-1 Hongo Bunkyo-ku Tokyo Japan
Tn6048	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6049	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6050	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6051	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6052	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6053	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6054	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6055	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6056	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6057	Dr Rob Van Houdt	Unit for Microbiology, SCK-CEN Boeretang 200 Mol Belgium
Tn6058	Dr Marina Mingoia	Department of Biomedical Sciences via Tronto 10/A Ancona Ancona Italy
Tn6059	Dr Emily Tili	Department of Biomedical Sciences via Tronto 10/A Ancona Ancona Italy
Tn6060	Dr Piklu Roy Chowdhury	University of Technology, Sydney Institute for the Biotechnology of Infectious Dis Faculty of Science, City Campus, Broadway Sydney NSW Australia
Tn6061	Mr Sebastien Coyne	Institut Pasteur Unité des Agents Antibactériens 25-28 rue du Docteur Roux Paris France
Tn6062	Mr Minh-Duy Phan	The Wellcome Trust Sanger Institute Wellcome Genome Campus Hinxton Cambridge United Kingdom
Tn6063	Dr Jaroslaw Krol	University of Idaho Department of Biological Sciences Life Science Building 252 Moscow Idaho USA
Tn6064	Dr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6065	Dr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6066	Dr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6067	Dr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6068	Dr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6069	Dr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6070	Dr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6071	Dr Michael Ryan	University of Limerick Department of Chemical and Environmental Sciences Castletroy Limerick Ireland
Tn6072	Dr Liang Chen	PHRI-NJMS, UMDNJ 225 Warren St W210M Newark NJ Unite States
Tn6073	Mr Michael Brouwer	UCL Eastman Dental Institute 256 Gray's Inn Road London UK
Tn6074	Dr Helena Seth-Smith	Wellcome Trust Sanger Insitute Hinxton Cambridge UK
Tn6075	Dr Helena Seth-Smith	Wellcome Trust Sanger Insitute Hinxton Cambridge UK
Tn6076	Dr Helena Seth-Smith	Wellcome Trust Sanger Insitute Hinxton Cambridge UK
Tn6077	Dr Helena Seth-Smith	Wellcome Trust Sanger Insitute Hinxton Cambridge UK

Credits

This site was designed by Sarah Jancich (UCL) and is maintained by Karen Widdowson (EDI, UCL). The database is administered and updated by Adam Roberts (EDI, UCL).

Disclaimer

The Eastman Dental Institute has taken all reasonable care to ensure this page was accurate on the stated date of publication or last modification. The Eastman Dental Institute takes no responsibility for the consequences of error or for any loss or damage suffered by users of any of the information published on any of these pages, and such information does not form any basis of a contract with readers or users of it.

It is in the nature of Web sites, many of which are experimental or constantly changing, that information published may be for test purposes only, may be out of date, or may be the personal opinion of the author. Readers should verify information gained from the Web with the appropriate Eastman Dental Institute authorities before relying on it.

Tn Number Registry

Assigned Tn numbers

Credits

Disclaimer