Dr. Remigio Picone


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My research focused on the investigation of the extrinsic and intrinsic control of shape and polarity in cells spreading and growing on micropatterned linear surfaces. More specifically, I used a combination of molecular genetics, RNAi, and micropatterning techniques to study the regulation of cell length in this system. Using micro-contact printing (µCP) to create protein adhesive areas, I defined two-dimensional shapes that could be reproducibly imposed on a cell, allowing the intrinsic (genetic) and extrinsic (chemical and mechanical) factors involved in the regulation of cell shape to be experimentally separated. It was then possible to generate precise quantitative comparisons of cytoskeletal architecture in populations of identically shaped cells, to test for example how microtubules in different RNAi treated cells probe the same cell space. Remi Picone's research image

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